TY - JOUR AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Kenéz, Balázs AU - Balogh Weiser, Diána TI - Nanoformulation of Therapeutic Enzymes: A Short Review JF - PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING J2 - PERIOD POLYTECH CHEM ENG VL - 67 PY - 2023 IS - 4 SP - 624 EP - 635 PG - 12 SN - 0324-5853 DO - 10.3311/PPch.22826 UR - https://m2.mtmt.hu/api/publication/34155138 ID - 34155138 N1 - Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Organic Chemistry Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 30 November 2023 Correspondence Address: Balogh-Weiser, D.; Department of Physical Chemistry and Materials Science, Műegyetem rkp. 3, Hungary; email: balogh.weiser.diana@vbk.bme.hu AB - Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too. LA - English DB - MTMT ER - TY - JOUR AU - Balogh Weiser, Diána AU - Molnár, Alexandra AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Szemes, József AU - Decsi, Balázs AU - Katona, Gábor AU - Salamah, Maryana AU - Ender, Ferenc AU - Kovács, Anita AU - Berkó, Szilvia AU - Budai-Szűcs, Mária AU - Balogh, György Tibor TI - Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 4 PG - 19 SN - 1999-4923 DO - 10.3390/pharmaceutics15041174 UR - https://m2.mtmt.hu/api/publication/33740967 ID - 33740967 N1 - Funding Agency and Grant Number: National Research Development and Innovation (NRDI) Fund [PD-131467]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00175/21]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [UNKP-22-3-1-BME-173]; Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund [TKP-9-8/PALY-2021]; European Union [RRF-2.3.1-21-2022-00015] Funding text: This research has been supported by the National Research Development and Innovation (NRDI) Fund via grant PD-131467 (D.B.W.). B.W.D. acknowledges the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21). The study was supported by the UNKP-22-3-1-BME-173 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. The research was performed in the frame of Project no. TKP-9-8/PALY-2021 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme and Project no. RRF-2.3.1-21-2022-00015 has been implemented with the support provided by the European Union. AB - The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications. LA - English DB - MTMT ER - TY - JOUR AU - Koplányi, Gábor AU - Bell, Evelin AU - Molnár, Zsófia Klára AU - Tóth, Gergő Dániel AU - Józó, Muriel AU - Szilágyi, András Ferenc AU - Ender, Ferenc AU - Pukánszky, Béla AU - Vértessy, Beáta (Grolmuszné) AU - Poppe, László AU - Balogh Weiser, Diána TI - Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning JF - CATALYSTS J2 - CATALYSTS VL - 11 PY - 2021 IS - 10 PG - 14 SN - 2073-4344 DO - 10.3390/catal11101149 UR - https://m2.mtmt.hu/api/publication/32242806 ID - 32242806 N1 - Export Date: 26 October 2021 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő Dániel AU - Nochta-Kazsoki, Adrienn Katalin AU - Gyarmati, Benjámin Sándor AU - Szilágyi, András Ferenc AU - Vasvári, Gábor AU - Katona, Gábor AU - Szente, Lajos AU - Zelkó, Romána AU - Poppe, László AU - Balogh Weiser, Diána AU - Balogh, György Tibor TI - Nanofibrous Formulation of Cyclodextrin Stabilized Lipases for Efficient Pancreatin Replacement Therapies JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 13 PY - 2021 IS - 7 PG - 18 SN - 1999-4923 DO - 10.3390/pharmaceutics13070972 UR - https://m2.mtmt.hu/api/publication/32082348 ID - 32082348 N1 - Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hőgyes Endre utca 7-9, Budapest, H-1092, Hungary Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei u. 98, Debrecen, H-4032, Hungary Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Cyclolab Cyclodextrin Research & Development Laboratory Ltd., Illatos út 7, Budapest, H-1097, Hungary Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Export Date: 6 September 2021 Correspondence Address: Balogh-Weiser, D.; Department of Physical Chemistry and Materials Science, Műegyetem rkp. 3, Hungary; email: balogh.weiser.diana@vbk.bme.hu Correspondence Address: Balogh, G.T.; Department of Chemical and Environmental Process Engineering, Műegyetem rkp. 3, Hungary; email: balogh.gyorgy@vbk.bme.hu Funding details: 2020-4.1.1-TKP2020 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding details: Innovációs és Technológiai Minisztérium, TKP2020-IKA-04 Funding details: National Research, Development and Innovation Office, PD-131467 Funding text 1: Funding: This research has been supported by National Research, Development and Innovation Office (Budapest, Hungary), which we acknowledge for the research’s funding (D.B.-W: PD-131467), and also supported by the NRDI Fund (TKP2020 IES, Grant No. BME-IE-BIO) based on the charter of bolster issued by the NRDI Office under the auspices of the Ministry for Innovation and Technology (Budapest, Hungary). Project no. TKP2020-IKA-04 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the 2020-4.1.1-TKP2020 funding scheme. LA - English DB - MTMT ER - TY - JOUR AU - Nagy-Győr, László AU - Farkas, Emese AU - Lăcătuș, Mihai AU - Tóth, Gergő Dániel AU - Incze, Dániel János AU - Hornyánszky, Gábor AU - Erdélyiné Bódai, Viktória AU - Paizs, Csaba AU - Poppe, László AU - Balogh Weiser, Diána TI - Conservation of the biocatalytic activity of whole yeast cells by supported sol-gel entrapment for efficient acyloin condensation JF - PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING J2 - PERIOD POLYTECH CHEM ENG VL - 64 PY - 2020 IS - 2 SP - 153 EP - 161 PG - 9 SN - 0324-5853 DO - 10.3311/PPch.14645 UR - https://m2.mtmt.hu/api/publication/30819582 ID - 30819582 N1 - Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, P. O. B. 91, Budapest, H-1521, Hungary Biocatalysis and Biotransformation Research Center, Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Cluj-Napoca, Ro-400028, Romania Fermentia Ltd, Berlini str. 45-47, Budapest, H-1045, Hungary Witaria Ltd, Luther str. 4-6, Budapest, H-1087, Hungary SynBiocat LLC, Szilasliget str. 3, Budapest, H-1172, Hungary Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, P. O. B. 91, Budapest, H-1521, Hungary Cited By :2 Export Date: 5 April 2022 Correspondence Address: Balogh-Weiser, D.; Department of Organic Chemistry and Technology, P. O. B. 91, Hungary; email: dweiser@mail.bme.hu Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: National Authority for Scientific Research and Innovation, ANCSI Funding details: European Cooperation in Science and Technology, COST, 103413, CM 1303, P37_273 Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding details: European Regional Development Fund, ERDF Funding text 1: Authors BWD and LP thanks the financial support for Excellence Program of the Ministry of Human Capacities in the frame of Biotechnology research area of Budapest University of Technology and Economics (BME FIKP-BIO). CP and LP thank the COST Action Funding text 2: Authors BWD and LP thanks the financial support for Excellence Program of the Ministry of Human Capacities in the frame of Biotechnology research area of Budapest University of Technology and Economics (BME FIKP-BIO). CP and LP thank the COST Action SysBiocat (CM 1303) and project NEMSyB, ID P37_273, Cod MySMIS 103413 [funded by National Authority for Scientific Research and Innovation (ANCSI, Bucharest, Romania) and European Regional Development Fund, Competitiveness Operational Program 2014-2020 (POC), Priority axis 1, Action 1.1] for support. AB - In this study, an efficient and generally applicable 2nd generation sol-gel entrapment method was developed for immobilization of yeast cells. Cells of Lodderomyces elongisporus, Candida norvegica, Debaryomyces fabryi, Pichia carsonii strains in admixture with hollow silica microspheres support were immobilized in sol-gel matrix obtained from polycondensation of tetraethoxysilane. As biocatalysts in the selective acyloin condensation of benzaldehyde catalyzed by pyruvate decarboxylase of the yeast, the novel immobilized whole-cell preparations were compared to other states of the cells such as freshly harvested wet cell paste, lyophilized cells and sol-gel entrapped preparations without hollow silica microspheres support. Reusability and storability studies designated this novel 2nd generation sol-gel method as a promising alternative for solid formulation of whole-cells bypassing expensive and difficult downstream steps while providing easy-to-handle and stable biocatalysts with long-term preservation of the biocatalytic activity. LA - English DB - MTMT ER -