TY - JOUR AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Kenéz, Balázs AU - Balogh Weiser, Diána TI - Nanoformulation of Therapeutic Enzymes: A Short Review JF - PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING J2 - PERIOD POLYTECH CHEM ENG VL - 67 PY - 2023 IS - 4 SP - 624 EP - 635 PG - 12 SN - 0324-5853 DO - 10.3311/PPch.22826 UR - https://m2.mtmt.hu/api/publication/34155138 ID - 34155138 N1 - Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Organic Chemistry Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 30 November 2023 Correspondence Address: Balogh-Weiser, D.; Department of Physical Chemistry and Materials Science, Műegyetem rkp. 3, Hungary; email: balogh.weiser.diana@vbk.bme.hu AB - Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too. LA - English DB - MTMT ER - TY - JOUR AU - Balogh Weiser, Diána AU - Molnár, Alexandra AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Szemes, József AU - Decsi, Balázs AU - Katona, Gábor AU - Salamah, Maryana AU - Ender, Ferenc AU - Kovács, Anita AU - Berkó, Szilvia AU - Budai-Szűcs, Mária AU - Balogh, György Tibor TI - Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 4 PG - 19 SN - 1999-4923 DO - 10.3390/pharmaceutics15041174 UR - https://m2.mtmt.hu/api/publication/33740967 ID - 33740967 N1 - Funding Agency and Grant Number: National Research Development and Innovation (NRDI) Fund [PD-131467]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00175/21]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [UNKP-22-3-1-BME-173]; Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund [TKP-9-8/PALY-2021]; European Union [RRF-2.3.1-21-2022-00015] Funding text: This research has been supported by the National Research Development and Innovation (NRDI) Fund via grant PD-131467 (D.B.W.). B.W.D. acknowledges the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21). The study was supported by the UNKP-22-3-1-BME-173 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. The research was performed in the frame of Project no. TKP-9-8/PALY-2021 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme and Project no. RRF-2.3.1-21-2022-00015 has been implemented with the support provided by the European Union. AB - The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications. LA - English DB - MTMT ER - TY - JOUR AU - Vincze, Anna AU - Dékány, Gergely AU - Bicsak, Richárd AU - Formanek, András AU - Moreau, Yves AU - Koplányi, Gábor AU - Takács, Gergely AU - Katona, Gábor AU - Balogh Weiser, Diána AU - Arany, Ádám AU - Balogh, György Tibor TI - Natural Lipid Extracts as an Artificial Membrane for Drug Permeability Assay: In Vitro and In Silico Characterization JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 3 PG - 19 SN - 1999-4923 DO - 10.3390/pharmaceutics15030899 UR - https://m2.mtmt.hu/api/publication/33692858 ID - 33692858 N1 - Funding Agency and Grant Number: Richter Gedeon Excellence PhD Scholarship from the Richter Gedeon Foundation [BO/00175/21]; Hungarian Academy of Sciences Funding text: This project was supported by the Central Europe Leuven Strategic Alliance (CELSA-2021/019) and by an FK-137582 grant from the National Research and Innovation Office, Hungary (NRDI). AB - In vitro non-cellular permeability models such as the parallel artificial membrane permeability assay (PAMPA) are widely applied tools for early-phase drug candidate screening. In addition to the commonly used porcine brain polar lipid extract for modeling the blood–brain barrier’s permeability, the total and polar fractions of bovine heart and liver lipid extracts were investigated in the PAMPA model by measuring the permeability of 32 diverse drugs. The zeta potential of the lipid extracts and the net charge of their glycerophospholipid components were also determined. Physicochemical parameters of the 32 compounds were calculated using three independent forms of software (Marvin Sketch, RDKit, and ACD/Percepta). The relationship between the lipid-specific permeabilities and the physicochemical descriptors of the compounds was investigated using linear correlation, Spearman correlation, and PCA analysis. While the results showed only subtle differences between total and polar lipids, permeability through liver lipids highly differed from that of the heart or brain lipid-based models. Correlations between the in silico descriptors (e.g., number of amide bonds, heteroatoms, and aromatic heterocycles, accessible surface area, and H-bond acceptor–donor balance) of drug molecules and permeability values were also found, which provides support for understanding tissue-specific permeability. LA - English DB - MTMT ER - TY - JOUR AU - Koplányi, Gábor AU - Bell, Evelin AU - Molnár, Zsófia Klára AU - Katona, Gábor AU - Neumann, Péter Lajos AU - Ender, Ferenc AU - Balogh, György Tibor AU - Žnidaršič-Plazl, Polona AU - Poppe, László AU - Balogh Weiser, Diána TI - Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase. Applying an Advanced Nanocomposite System TS - Applying an Advanced Nanocomposite System JF - CHEMBIOCHEM J2 - CHEMBIOCHEM VL - 24 PY - 2023 IS - 7 PG - 11 SN - 1439-4227 DO - 10.1002/cbic.202200713 UR - https://m2.mtmt.hu/api/publication/33574611 ID - 33574611 LA - English DB - MTMT ER - TY - JOUR AU - Balogh Weiser, Diána AU - Poppe, László AU - Kenéz, Balázs AU - Decsi, Balázs AU - Koplányi, Gábor AU - Katona, Gábor AU - Gyarmati, Benjámin Sándor AU - Ender, Ferenc AU - Balogh, György Tibor TI - Novel biomimetic nanocomposite for investigation of drug metabolism JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 368 PY - 2022 IS - Part B PG - 10 SN - 0167-7322 DO - 10.1016/j.molliq.2022.120781 UR - https://m2.mtmt.hu/api/publication/33229099 ID - 33229099 N1 - Funding Agency and Grant Number: NRDI Fund (TKP2020 NC); Ministry for Innovation and Technology; New National Excellence Program of the Ministry of Human Capacities and OTKA PD grant [131467]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; New National Excellence Program of the Ministry of Human Capacities; OTKA PD grant by the NRDI Office; Servier-Beregi Foundation; [BME-NC]; [BO/00175/21]; [UNKP-21-5 (UNKP-21-5-BME-386)] Funding text: The research reported in this paper and carried out at BME has been supported by the NRDI Fund (TKP2020 NC, Grant No. BME-NC) based on the charter of bolster issued by the NRDI Office under the auspices of the Ministry for Innovation and Technology. D.B.W. acknowledges the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21) , the UNKP-21-5 (UNKP-21-5-BME-386) New National Excellence Program of the Ministry of Human Capacities and OTKA PD grant (131467) by the NRDI Office. B.D. thanks Servier-Beregi Foundation for the support of this research. Part number: B AB - In vitro mimicking of hepatic drug metabolism is a key issue in early-stage drug discovery. Synthetic metalloporphyrins show structural similarity with the heme type prosthetic group of cytochrome P450 as primary hepatic enzyme in oxidative drug biotransformation. Therefore, they can catalyze these oxidations. Concerning economical aspects and the poor stability of metalloporphyrin, their immobilization onto or into solid carriers can be promising solution. This study presents a novel immobilized metalloporphyrin nanocomposite system and its potential use as biomimetic catalysts. The developed two-step immobilization procedure consists of two main steps. First, the ionic binding of meso-tetra (parasulphonatophenyl) iron porphyrin onto functionalized magnetic nanoparticles is established, followed by embedding the nanoparticles into polylactic acid nanofibers by electrospinning technique. Due to the synergistic morphological and chemo-structural advantages of binding onto nanoparticles and embedding in polymeric matrices the biomimetic efficiency of metalloporphyrin can be remarkably enhanced, while substrate conversion value was tenfold larger than which could be achieved with classic human liver microsomal system. LA - English DB - MTMT ER - TY - JOUR AU - Koplányi, Gábor AU - Bell, Evelin AU - Molnár, Zsófia Klára AU - Tóth, Gergő Dániel AU - Józó, Muriel AU - Szilágyi, András Ferenc AU - Ender, Ferenc AU - Pukánszky, Béla AU - Vértessy, Beáta (Grolmuszné) AU - Poppe, László AU - Balogh Weiser, Diána TI - Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning JF - CATALYSTS J2 - CATALYSTS VL - 11 PY - 2021 IS - 10 PG - 14 SN - 2073-4344 DO - 10.3390/catal11101149 UR - https://m2.mtmt.hu/api/publication/32242806 ID - 32242806 N1 - Export Date: 26 October 2021 LA - English DB - MTMT ER -