@article{MTMT:34155138,
	title = {Nanoformulation of Therapeutic Enzymes: A Short Review},
	url = {https://m2.mtmt.hu/api/publication/34155138},
	author = {Tóth, Gergő Dániel and Koplányi, Gábor and Kenéz, Balázs and Balogh Weiser, Diána},
	doi = {10.3311/PPch.22826},
	journal-iso = {PERIOD POLYTECH CHEM ENG},
	journal = {PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING},
	volume = {67},
	unique-id = {34155138},
	issn = {0324-5853},
	abstract = {Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too.},
	year = {2023},
	eissn = {1587-3765},
	pages = {624-635},
	orcid-numbers = {Koplányi, Gábor/0000-0002-3791-1057; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:33740967,
	title = {Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique},
	url = {https://m2.mtmt.hu/api/publication/33740967},
	author = {Balogh Weiser, Diána and Molnár, Alexandra and Tóth, Gergő Dániel and Koplányi, Gábor and Szemes, József and Decsi, Balázs and Katona, Gábor and Salamah, Maryana and Ender, Ferenc and Kovács, Anita and Berkó, Szilvia and Budai-Szűcs, Mária and Balogh, György Tibor},
	doi = {10.3390/pharmaceutics15041174},
	journal-iso = {PHARMACEUTICS},
	journal = {PHARMACEUTICS},
	volume = {15},
	unique-id = {33740967},
	issn = {1999-4923},
	abstract = {The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications.},
	keywords = {PROTEIN; Therapy; EFFICACY; ENZYMATIC RESOLUTION; LIPASE; acne vulgaris; Electrospinning; nanoformulation; Skin treatment; Immobilized lipase; nano mask},
	year = {2023},
	eissn = {1999-4923},
	orcid-numbers = {Balogh Weiser, Diána/0000-0002-9957-1203; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Kovács, Anita/0000-0001-5593-1329; Berkó, Szilvia/0000-0002-3842-8876; Budai-Szűcs, Mária/0000-0001-5187-5702; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:33692858,
	title = {Natural Lipid Extracts as an Artificial Membrane for Drug Permeability Assay: In Vitro and In Silico Characterization},
	url = {https://m2.mtmt.hu/api/publication/33692858},
	author = {Vincze, Anna and Dékány, Gergely and Bicsak, Richárd and Formanek, András and Moreau, Yves and Koplányi, Gábor and Takács, Gergely and Katona, Gábor and Balogh Weiser, Diána and Arany, Ádám and Balogh, György Tibor},
	doi = {10.3390/pharmaceutics15030899},
	journal-iso = {PHARMACEUTICS},
	journal = {PHARMACEUTICS},
	volume = {15},
	unique-id = {33692858},
	issn = {1999-4923},
	abstract = {In vitro non-cellular permeability models such as the parallel artificial membrane permeability assay (PAMPA) are widely applied tools for early-phase drug candidate screening. In addition to the commonly used porcine brain polar lipid extract for modeling the blood–brain barrier’s permeability, the total and polar fractions of bovine heart and liver lipid extracts were investigated in the PAMPA model by measuring the permeability of 32 diverse drugs. The zeta potential of the lipid extracts and the net charge of their glycerophospholipid components were also determined. Physicochemical parameters of the 32 compounds were calculated using three independent forms of software (Marvin Sketch, RDKit, and ACD/Percepta). The relationship between the lipid-specific permeabilities and the physicochemical descriptors of the compounds was investigated using linear correlation, Spearman correlation, and PCA analysis. While the results showed only subtle differences between total and polar lipids, permeability through liver lipids highly differed from that of the heart or brain lipid-based models. Correlations between the in silico descriptors (e.g., number of amide bonds, heteroatoms, and aromatic heterocycles, accessible surface area, and H-bond acceptor–donor balance) of drug molecules and permeability values were also found, which provides support for understanding tissue-specific permeability.},
	year = {2023},
	eissn = {1999-4923},
	orcid-numbers = {Formanek, András/0000-0003-0734-1417; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Balogh Weiser, Diána/0000-0002-9957-1203; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:33574611,
	title = {Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase. Applying an Advanced Nanocomposite System},
	url = {https://m2.mtmt.hu/api/publication/33574611},
	author = {Koplányi, Gábor and Bell, Evelin and Molnár, Zsófia Klára and Katona, Gábor and Neumann, Péter Lajos and Ender, Ferenc and Balogh, György Tibor and Žnidaršič-Plazl, Polona and Poppe, László and Balogh Weiser, Diána},
	doi = {10.1002/cbic.202200713},
	journal-iso = {CHEMBIOCHEM},
	journal = {CHEMBIOCHEM},
	volume = {24},
	unique-id = {33574611},
	issn = {1439-4227},
	year = {2023},
	eissn = {1439-7633},
	orcid-numbers = {Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Neumann, Péter Lajos/0000-0002-2881-5733; Ender, Ferenc/0000-0003-3800-5707; Balogh, György Tibor/0000-0003-3347-1880; Poppe, László/0000-0002-8358-1378; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:33229099,
	title = {Novel biomimetic nanocomposite for investigation of drug metabolism},
	url = {https://m2.mtmt.hu/api/publication/33229099},
	author = {Balogh Weiser, Diána and Poppe, László and Kenéz, Balázs and Decsi, Balázs and Koplányi, Gábor and Katona, Gábor and Gyarmati, Benjámin Sándor and Ender, Ferenc and Balogh, György Tibor},
	doi = {10.1016/j.molliq.2022.120781},
	journal-iso = {J MOL LIQ},
	journal = {JOURNAL OF MOLECULAR LIQUIDS},
	volume = {368},
	unique-id = {33229099},
	issn = {0167-7322},
	abstract = {In vitro mimicking of hepatic drug metabolism is a key issue in early-stage drug discovery. Synthetic metalloporphyrins show structural similarity with the heme type prosthetic group of cytochrome P450 as primary hepatic enzyme in oxidative drug biotransformation. Therefore, they can catalyze these oxidations. Concerning economical aspects and the poor stability of metalloporphyrin, their immobilization onto or into solid carriers can be promising solution. This study presents a novel immobilized metalloporphyrin nanocomposite system and its potential use as biomimetic catalysts. The developed two-step immobilization procedure consists of two main steps. First, the ionic binding of meso-tetra (parasulphonatophenyl) iron porphyrin onto functionalized magnetic nanoparticles is established, followed by embedding the nanoparticles into polylactic acid nanofibers by electrospinning technique. Due to the synergistic morphological and chemo-structural advantages of binding onto nanoparticles and embedding in polymeric matrices the biomimetic efficiency of metalloporphyrin can be remarkably enhanced, while substrate conversion value was tenfold larger than which could be achieved with classic human liver microsomal system.},
	keywords = {IMMOBILIZATION; COMPOSITES; OXIDATION; NANOFIBERS; Biotransformation; Chemistry, Physical; FABRICATION; magnetic nanoparticles; Metalloporphyrins; CATALYTIC-ACTIVITY},
	year = {2022},
	eissn = {1873-3166},
	orcid-numbers = {Balogh Weiser, Diána/0000-0002-9957-1203; Poppe, László/0000-0002-8358-1378; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Ender, Ferenc/0000-0003-3800-5707; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:32242806,
	title = {Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning},
	url = {https://m2.mtmt.hu/api/publication/32242806},
	author = {Koplányi, Gábor and Bell, Evelin and Molnár, Zsófia Klára and Tóth, Gergő Dániel and Józó, Muriel and Szilágyi, András Ferenc and Ender, Ferenc and Pukánszky, Béla and Vértessy, Beáta (Grolmuszné) and Poppe, László and Balogh Weiser, Diána},
	doi = {10.3390/catal11101149},
	journal-iso = {CATALYSTS},
	journal = {CATALYSTS},
	volume = {11},
	unique-id = {32242806},
	year = {2021},
	eissn = {2073-4344},
	orcid-numbers = {Koplányi, Gábor/0000-0002-3791-1057; Szilágyi, András Ferenc/0000-0001-6898-1755; Ender, Ferenc/0000-0003-3800-5707; Poppe, László/0000-0002-8358-1378; Balogh Weiser, Diána/0000-0002-9957-1203}
}