TY - GEN AU - Kurilla, Anita AU - László, Loretta AU - Takács, Tamás AU - Tilajka, Álmos AU - Laura, Lukacs AU - Julianna, Novak AU - Pancsa, Rita AU - Buday, László AU - Vas, Virág TI - The regulatory role of the CD2AP/TKS4 interaction in EMT and its potential as a biomarker for colon cancer PY - 2023 UR - https://m2.mtmt.hu/api/publication/34768197 ID - 34768197 LA - English DB - MTMT ER - TY - JOUR AU - Kurilla, Anita AU - László, Loretta AU - Takács, Tamás AU - Tilajka, Álmos AU - Lukács, L. AU - Novák, J. AU - Pancsa, Rita AU - Buday, László AU - Vas, Virág TI - Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 20 PG - 17 SN - 1661-6596 DO - 10.3390/ijms242015136 UR - https://m2.mtmt.hu/api/publication/34266900 ID - 34266900 LA - English DB - MTMT ER - TY - JOUR AU - László, Loretta AU - Maczelka, Hédi AU - Takács, Tamás AU - Kurilla, Anita AU - Tilajka, Álmos AU - Buday, László AU - Vas, Virág AU - Apáti, Ágota TI - A Novel Cell-Based Model for a Rare Disease: The Tks4-KO Human Embryonic Stem Cell Line as a Frank-Ter Haar Syndrome Model System JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms23158803 UR - https://m2.mtmt.hu/api/publication/33063952 ID - 33063952 N1 - Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary National Laboratory for Drug Research and Development, Budapest, 1117, Hungary Doctoral School of Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, 1117, Hungary Basic and Translational Medicine Doctoral School, Semmelweis University, Budapest, 1085, Hungary Export Date: 9 December 2022 Correspondence Address: Vas, V.; Institute of Enzymology, Hungary; email: vas.virag@ttk.hu Correspondence Address: Apáti, Á.; Institute of Enzymology, Hungary; email: apati.agota@ttk.hu AB - Tyrosine kinase substrate with four SH3 domains (Tks4) scaffold protein plays roles in cell migration and podosome formation and regulates systemic mechanisms such as adult bone homeostasis and adipogenesis. Mutations in the Tks4 gene (SH3PXD2b) cause a rare developmental disorder called Frank-Ter Haar syndrome (FTHS), which leads to heart abnormalities, bone tissue defects, and reduced adiposity. We aimed to produce a human stem cell-based in vitro FTHS model system to study the effects of the loss of the Tks4 protein in different cell lineages and the accompanying effects on the cell signalome. To this end, we used CRISPR/Cas9 (clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated (Cas9)) to knock out the SH3PXD2b gene in the HUES9 human embryonic stem cell line (hESC), and we obtained stable homo- and heterozygous knock out clones for use in studying the potential regulatory roles of Tks4 protein in embryonic stem cell biology. Based on pluripotency marker measurements and spontaneous differentiation capacity assays, we concluded that the newly generated Tks4-KO HUES9 cells retained their embryonic stem cell characteristics. We propose that the Tks4-KO HUES9 cells could serve as a tool for further cell differentiation studies to investigate the involvement of Tks4 in the complex disorder FTHS. Moreover, we successfully differentiated all of the clones into mesenchymal stem cells (MSCs). The derived MSC cultures showed mesenchymal morphology and expressed MSC markers, although the expression levels of mesodermal and osteogenic marker genes were reduced, and several EMT (epithelial mesenchymal transition)-related features were altered in the Tks4-KO MSCs. Our results suggest that the loss of Tks4 leads to FTHS by altering cell lineage differentiation and cell maturation processes, rather than by regulating embryonic stem cell potential. LA - English DB - MTMT ER - TY - JOUR AU - Sipeki, Szabolcs AU - Koprivanacz, Kitti AU - Takács, Tamás AU - Kurilla, Anita AU - László, Loretta AU - Vas, Virag AU - Buday, László TI - Novel Roles of SH2 and SH3 Domains in Lipid Binding JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 5 PG - 16 SN - 2073-4409 DO - 10.3390/cells10051191 UR - https://m2.mtmt.hu/api/publication/32020389 ID - 32020389 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund of Hungary [K124045, FIEK_16-1-2016-0005, HunProtEx 2018-1.2.1-NKP-2018-00005]; MedinProt Program of the Hungarian Academy of Sciences [K 139100]; Janos Bolyai Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences Funding text: This research was funded by grants from the National Research, Development and Innovation Fund of Hungary (K124045, FIEK_16-1-2016-0005, HunProtEx 2018-1.2.1-NKP-2018-00005, L.B.), the MedinProt Program of the Hungarian Academy of Sciences (L.B.), (K 139100) and the Janos Bolyai Scholarship of the Hungarian Academy of Sciences (V.V.). LA - English DB - MTMT ER - TY - JOUR AU - László, Loretta AU - Kurilla, Anita AU - Takács, Tamás AU - Kudlik, Gyöngyi AU - Koprivanacz, Kitti AU - Buday, László AU - Vas, Virág TI - Recent updates on the significance of KRAS mutations in colorectal cancer biology JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 3 PG - 20 SN - 2073-4409 DO - 10.3390/cells10030667 UR - https://m2.mtmt.hu/api/publication/31909245 ID - 31909245 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund of Hungary [K 124045, FIEK_16-1-2016-0005, HunProtEx 2018-1.2.1-NKP-2018-00005]; MedinProt Program of the Hungarian Academy of Sciences Funding text: This work was supported by grants from the National Research, Development and Innovation Fund of Hungary (K 124045, FIEK_16-1-2016-0005, HunProtEx 2018-1.2.1-NKP-2018-00005, L.B.) and the MedinProt Program of the Hungarian Academy of Sciences (L.B.). LA - English DB - MTMT ER - TY - CHAP AU - Uray, Iván AU - László, Loretta ED - Ray, Swapan K. TI - Translation of Effects of Retinoids and Rexinoids: Extraction and Quality Assessment of RNA from Formalin-Fixed Tissues T2 - Retinoid and Rexinoid Signaling PB - Springer New York SN - 9781493995844 T3 - Methods in Molecular Biology, ISSN 1064-3745 ; 2019. PY - 2019 SP - 225 EP - 236 PG - 12 DO - 10.1007/978-1-4939-9585-1_16 UR - https://m2.mtmt.hu/api/publication/31240686 ID - 31240686 AB - Retinoids and rexinoids directly and selectively activate their nuclear receptors, resulting in changes in the transcript levels of their target genes. Consequently, quantitating mRNA levels transcribed from cognate target genes is the most accurate measure of retinoid action. These changes can serve as relevant endpoints in biomarker trials, as well as in vivo preclinical studies. In gene expression analyses of archival material such as formalin-fixed paraffin-embedded (FFPE) tissues, assessing the quality of the extracted RNA is essential for the validation of the studies. With next generation sequencing (NGS) becoming the method of choice for gene expression profiling, RNA quality has become a critical aspect of study feasibility. In this chapter, we describe a method to extract RNA and to assess the intactness of RNA samples extracted from paraffin-embedded tissues. LA - English DB - MTMT ER -