TY - JOUR AU - Mohammad Aslam, Sabit AU - Vass, Imre AU - Szabó, Milán TI - Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 10 PG - 14 SN - 1661-6596 DO - 10.3390/ijms24108712 UR - https://m2.mtmt.hu/api/publication/34043896 ID - 34043896 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office (NKFIH) [FK128977]; Biological Research Centre, Szeged, Hungary Funding text: This research was funded by The National Research, Development and Innovation Office (NKFIH), grant number FK128977, and the APC was funded by Biological Research Centre, Szeged, Hungary. AB - The dinoflagellate algae, Symbiodiniaceae, are significant symbiotic partners of corals due to their photosynthetic capacity. The photosynthetic processes of the microalgae consist of linear electron transport, which provides the energetic balance of ATP and NADPH production for CO2 fixation, and alternative electron transport pathways, including cyclic electron flow, which ensures the elevated ATP requirements under stress conditions. Flash-induced chlorophyll fluorescence relaxation is a non-invasive tool to assess the various electron transport pathways. A special case of fluorescence relaxation, the so-called wave phenomenon, was found to be associated with the activity of NAD(P)H dehydrogenase (NDH) in microalgae. We showed previously that the wave phenomenon existed in Symbiodiniaceae under acute heat stress and microaerobic conditions, however, the electron transport processes related to the wave phenomenon remained unknown. In this work, using various inhibitors, we show that (i) the linear electron transport has a crucial role in the formation of the wave, (ii) the inhibition of the donor side of Photosystem II did not induce the wave, whereas inhibition of the Calvin-Benson cycle accelerated it, (iii) the wave phenomenon was related to the operation of type II NDH (NDH-2). We therefore propose that the wave phenomenon is an important marker of the regulation of electron transport in Symbiodiniaceae. LA - English DB - MTMT ER - TY - JOUR AU - Mohammad Aslam, Sabit AU - Patil, Priyanka Pradeep AU - Vass, Imre AU - Szabó, Milán TI - Heat-Induced Photosynthetic Responses of Symbiodiniaceae Revealed by Flash-Induced Fluorescence Relaxation Kinetics JF - FRONTIERS IN MARINE SCIENCE J2 - FRONT MAR SCI VL - 9 PY - 2022 SN - 2296-7745 DO - 10.3389/fmars.2022.932355 UR - https://m2.mtmt.hu/api/publication/33025641 ID - 33025641 N1 - Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Climate Change Cluster, University of Technology Sydney, Ultimo, NSW, Australia Export Date: 15 August 2022 Correspondence Address: Vass, I.; Institute of Plant Biology, Hungary; email: vass.imre@brc.hu Correspondence Address: Szabó, M.; Institute of Plant Biology, Hungary; email: szabo.milan@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Patil, Priyanka Pradeep AU - Mohammad Aslam, Sabit AU - Vass, Imre AU - Szabó, Milán TI - Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii JF - PHOTOSYNTHESIS RESEARCH J2 - PHOTOSYNTH RES VL - 152 PY - 2022 SP - 235 EP - 244 PG - 10 SN - 0166-8595 DO - 10.1007/s11120-022-00900-3 UR - https://m2.mtmt.hu/api/publication/32677799 ID - 32677799 N1 - Funding Agency and Grant Number: ELKH Biological Research Center; National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [NKFIH FK128977]; Hungarian Academy of Sciences, MTA Premium Postdoctoral Research Program [PREMIUM-2017-38] Funding text: Open access funding provided by ELKH Biological Research Center. The work was supported by the National Research, Development and Innovation Office (NKFIH FK128977) and the Hungarian Academy of Sciences, MTA Premium Postdoctoral Research Program (Grant ID: PREMIUM-2017-38). LA - English DB - MTMT ER - TY - JOUR AU - Yadav, Ranay Mohan AU - Mohammad Aslam, Sabit AU - Madireddi, Sai Kiran AU - Chouhan, Nisha AU - Subramanyam, Rajagopal TI - Role of cyclic electron transport mutations pgrl1 and pgr5 in acclimation process to high light in Chlamydomonas reinhardtii JF - PHOTOSYNTHESIS RESEARCH J2 - PHOTOSYNTH RES VL - 146 PY - 2020 IS - 1-3 SP - 247 EP - 258 PG - 12 SN - 0166-8595 DO - 10.1007/s11120-020-00751-w UR - https://m2.mtmt.hu/api/publication/31469338 ID - 31469338 N1 - Cited By :1 Export Date: 25 May 2021 CODEN: PHRSD Correspondence Address: Subramanyam, R.; Department of Plant Sciences, India; email: srgsl@uohyd.ernet.in AB - Light is crucial for photosynthesis, but the amount of light that exceeds an organism's assimilation efficacy can lead to photo-oxidative damage and even cell death. In Chlamydomonas (C). reinhardtii cyclic electron flow (CEF) is very important for the elicitation of non-photochemical quenching (NPQ) by controlling the acidification of thylakoid lumen. This process requires the cooperation of proton gradient regulation (PGR) proteins, PGRL1 and PGR5. Here, we compared the growth pattern and photosynthetic activity between wild type (137c, t222+) and mutants impaired in CEF (pgrl1 and pgr5) under photoautotrophic and photoheterotrophic conditions. We have observed the discriminative expression of NPQ in the mutants impaired in CEF of pgrl1 and pgr5. The results obtained from the mutants showed reduced cell growth and density, Chl a/b ratio, fluorescence, electron transport rate, and yield of photosystem (PS)II. These mutants have reduced capability to develop a strong NPQ indicating that the role of CEF is very crucial for photoprotection. Moreover, the CEF mutant exhibits increased photosensitivity compared with the wild type. Therefore, we suggest that besides NPQ, the fraction of non-regulated non-photochemical energy loss (NO) also plays a crucial role during high light acclimation despite a low growth rate. This low NPQ rate may be due to less influx of protons coming from the CEF in cases of pgrl1 and pgr5 mutants. These results are discussed in terms of the relative photoprotective benefit, related to the thermal dissipation of excess light in photoautotrophic and photoheterotrophic conditions. LA - English DB - MTMT ER -