@mastersthesis{MTMT:36447000,
	title = {The role and significance of alternative electron transport in microalgae},
	url = {https://m2.mtmt.hu/api/publication/36447000},
	author = {Mohammad Aslam, Sabit},
	doi = {10.14232/phd.12466},
	publisher = {Universití of Szeged},
	unique-id = {36447000},
	year = {2025}
}

@article{MTMT:34043896,
	title = {Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae},
	url = {https://m2.mtmt.hu/api/publication/34043896},
	author = {Mohammad Aslam, Sabit and Vass, Imre and Szabó, Milán},
	doi = {10.3390/ijms24108712},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34043896},
	issn = {1661-6596},
	abstract = {The dinoflagellate algae, Symbiodiniaceae, are significant symbiotic partners of corals due to their photosynthetic capacity. The photosynthetic processes of the microalgae consist of linear electron transport, which provides the energetic balance of ATP and NADPH production for CO2 fixation, and alternative electron transport pathways, including cyclic electron flow, which ensures the elevated ATP requirements under stress conditions. Flash-induced chlorophyll fluorescence relaxation is a non-invasive tool to assess the various electron transport pathways. A special case of fluorescence relaxation, the so-called wave phenomenon, was found to be associated with the activity of NAD(P)H dehydrogenase (NDH) in microalgae. We showed previously that the wave phenomenon existed in Symbiodiniaceae under acute heat stress and microaerobic conditions, however, the electron transport processes related to the wave phenomenon remained unknown. In this work, using various inhibitors, we show that (i) the linear electron transport has a crucial role in the formation of the wave, (ii) the inhibition of the donor side of Photosystem II did not induce the wave, whereas inhibition of the Calvin-Benson cycle accelerated it, (iii) the wave phenomenon was related to the operation of type II NDH (NDH-2). We therefore propose that the wave phenomenon is an important marker of the regulation of electron transport in Symbiodiniaceae.},
	keywords = {INHIBITION; PHOTOSYSTEM-II; PHOTOSYNTHESIS; CHLOROPHYLL FLUORESCENCE; CHLOROPHYLL FLUORESCENCE; Biochemistry & Molecular Biology; symbiotic microalgae; linear electron flow},
	year = {2023},
	eissn = {1422-0067}
}

@article{MTMT:32677799,
	title = {Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii},
	url = {https://m2.mtmt.hu/api/publication/32677799},
	author = {Patil, Priyanka Pradeep and Mohammad Aslam, Sabit and Vass, Imre and Szabó, Milán},
	doi = {10.1007/s11120-022-00900-3},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	volume = {152},
	unique-id = {32677799},
	issn = {0166-8595},
	abstract = {Flash-induced chlorophyll fluorescence relaxation is a powerful tool to monitor the reoxidation reactions of the reduced primary quinone acceptor, Q<jats:sub>A</jats:sub><jats:sup>−</jats:sup> by Q<jats:sub>B</jats:sub> and the plastoquinone (PQ) pool, as well as the charge recombination reactions between the donor and acceptor side components of Photosystem II (PSII). Under certain conditions, when the PQ pool is highly reduced (e.g. in microaerobic conditions), a wave phenomenon appears in the fluorescence relaxation kinetics, which reflects the transient reoxidation and re-reduction of Q<jats:sub>A</jats:sub><jats:sup>−</jats:sup> by various electron transfer processes, which in cyanobacteria is mediated by NAD(P)H dehydrogenase (NDH-1). The wave phenomenon was also observed and assigned to the operation of type 2 NAD(P)H dehydrogenase (NDH-2) in the green alga <jats:italic>Chlamydomonas reinhardtii</jats:italic> under hydrogen-producing conditions, which required a long incubation of algae under sulphur deprivation (Krishna et al. J Exp Bot 70 (21):6321–6336, 2019). However, the conditions that induce the wave remained largely uncharacterized so far in microalgae. In this work, we investigated the wave phenomenon in <jats:italic>Chlamydomonas reinhardtii</jats:italic> under conditions that lead to a decrease of PSII activity by applying hydroxylamine treatment, which impacts the donor side of PSII in combination with a strongly reducing environment of the PQ pool (microaerobic conditions). A similar wave phenomenon could be induced by photoinhibitory conditions (illumination with strong light in the presence of the protein synthesis inhibitor lincomycin). These results indicate that the fluorescence wave phenomenon is activated in green algae when the PSII activity decreases relative to Photosystem I (PS I) activity and the PQ pool is strongly reduced. Therefore, the fluorescence wave could be used as a sensitive indicator of altered intersystem electron transfer processes, e.g. under stress conditions.},
	year = {2022},
	eissn = {1573-5079},
	pages = {235-244}
}

@article{MTMT:33025641,
	title = {Heat-Induced Photosynthetic Responses of Symbiodiniaceae Revealed by Flash-Induced Fluorescence Relaxation Kinetics},
	url = {https://m2.mtmt.hu/api/publication/33025641},
	author = {Mohammad Aslam, Sabit and Patil, Priyanka Pradeep and Vass, Imre and Szabó, Milán},
	doi = {10.3389/fmars.2022.932355},
	journal-iso = {FRONT MAR SCI},
	journal = {FRONTIERS IN MARINE SCIENCE},
	volume = {9},
	unique-id = {33025641},
	abstract = {Symbiodiniaceae live in endosymbiosis with corals. In the last few decades, mass bleaching events have occurred in the coral reefs, causing damage in the ecosystem and the associated species. Global temperature increase is affecting the algae, disturbing the whole symbiosis and leads to coral bleaching. However, the heat tolerance is strongly determined by the species (formerly genetic clades) harbored by the coral host. We assessed three different strains of Symbiodiniaceae family, i.e., <jats:italic>Fugacium kawagutii</jats:italic> (CS156), <jats:italic>Symbiodinium tridacnidorum</jats:italic> (2465), and <jats:italic>Symbiodinium microadriaticum</jats:italic> (2467), which display different heat tolerance under heat stress conditions. Flash-induced chlorophyll fluorescence relaxation is a useful tool to monitor various components of the photosynthetic electron transport chain and the redox reactions of plastoquinone pool. We observed the appearance of a wave phenomenon in the fluorescence relaxation by heating the strains in combination with microaerobic conditions. The characteristics of this fluorescence wave were found to be strain-specific and possibly related to the transient oxidation and re-reduction of the plastoquinone pool. The appearance of the wave phenomenon appears to be related to cyclic electron flow as well because it is accompanied with enhanced post-illumination chlorophyll fluorescence rise. These results will potentially reveal further details of the role of cyclic electron transport in Symbiodiniaceae and its relevance in heat stress tolerance.},
	year = {2022},
	eissn = {2296-7745}
}

@article{MTMT:31469338,
	title = {Role of cyclic electron transport mutations pgrl1 and pgr5 in acclimation process to high light in Chlamydomonas reinhardtii},
	url = {https://m2.mtmt.hu/api/publication/31469338},
	author = {Yadav, Ranay Mohan and Mohammad Aslam, Sabit and Madireddi, Sai Kiran and Chouhan, Nisha and Subramanyam, Rajagopal},
	doi = {10.1007/s11120-020-00751-w},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	volume = {146},
	unique-id = {31469338},
	issn = {0166-8595},
	abstract = {Light is crucial for photosynthesis, but the amount of light that exceeds an organism's assimilation efficacy can lead to photo-oxidative damage and even cell death. In Chlamydomonas (C). reinhardtii cyclic electron flow (CEF) is very important for the elicitation of non-photochemical quenching (NPQ) by controlling the acidification of thylakoid lumen. This process requires the cooperation of proton gradient regulation (PGR) proteins, PGRL1 and PGR5. Here, we compared the growth pattern and photosynthetic activity between wild type (137c, t222+) and mutants impaired in CEF (pgrl1 and pgr5) under photoautotrophic and photoheterotrophic conditions. We have observed the discriminative expression of NPQ in the mutants impaired in CEF of pgrl1 and pgr5. The results obtained from the mutants showed reduced cell growth and density, Chl a/b ratio, fluorescence, electron transport rate, and yield of photosystem (PS)II. These mutants have reduced capability to develop a strong NPQ indicating that the role of CEF is very crucial for photoprotection. Moreover, the CEF mutant exhibits increased photosensitivity compared with the wild type. Therefore, we suggest that besides NPQ, the fraction of non-regulated non-photochemical energy loss (NO) also plays a crucial role during high light acclimation despite a low growth rate. This low NPQ rate may be due to less influx of protons coming from the CEF in cases of pgrl1 and pgr5 mutants. These results are discussed in terms of the relative photoprotective benefit, related to the thermal dissipation of excess light in photoautotrophic and photoheterotrophic conditions.},
	keywords = {CHLOROPHYLL FLUORESCENCE; non-photochemical quenching; Chlamydomonas reinhardtii; High light; photosystems; Cyclic electron transport},
	year = {2020},
	eissn = {1573-5079},
	pages = {247-258},
	orcid-numbers = {Subramanyam, Rajagopal/0000-0003-3872-8390}
}