@article{MTMT:34567532,
	title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches},
	url = {https://m2.mtmt.hu/api/publication/34567532},
	author = {Welsh, Joshua A. and Goberdhan, Deborah C. I. and O'Driscoll, Lorraine and Buzás, Edit Irén and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Di Vizio, Dolores and Driedonks, Tom A. P. and Erdbrügger, Uta and Falcon‐Perez, Juan M. and Fu, Qing‐Ling and Hill, Andrew F. and Lenassi, Metka and Lim, Sai Kiang and Mahoney, Mỹ G. and Mohanty, Sujata and Möller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C. and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M. and Brucale, Marco and Burger, Dylan and Carney, Randy P. and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L. and Haughey, Norman J. and Hendrix, An and Ivanov, Alexander R. and Jovanovic‐Talisman, Tijana and Kruh‐Garcia, Nicole A. and Ku'ulei‐Lyn Faustino, Vroniqa and Kyburz, Diego and Lässer, Cecilia and Lennon, Kathleen M. and Lötvall, Jan and Maddox, Adam L. and Martens‐Uzunova, Elena S. and Mizenko, Rachel R. and Newman, Lauren A. and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S. and Saugstad, Julie A. and Shekari, Faezeh and Swift, Simon and Ter‐Ovanesyan, Dmitry and Tosar, Juan P. and Useckaite, Zivile and Valle, Francesco and Varga, Zoltán and van der Pol, Edwin and van Herwijnen, Martijn J. C. and Wauben, Marca H. M. and Wehman, Ann M. and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J. and Théry, Clotilde and Witwer, Kenneth W. and Haseeb, Zubair},
	doi = {10.1002/jev2.12404},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {13},
	unique-id = {34567532},
	abstract = {Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.},
	year = {2024},
	eissn = {2001-3078},
	orcid-numbers = {Welsh, Joshua A./0000-0002-1097-9756; Goberdhan, Deborah C. I./0000-0003-0645-6714; Buzás, Edit Irén/0000-0002-3744-206X; Bussolati, Benedetta/0000-0002-3663-5134; Cai, Houjian/0000-0003-4887-2652; Falcon‐Perez, Juan M./0000-0003-3133-0670; Hill, Andrew F./0000-0001-5581-2354; Lenassi, Metka/0000-0002-9488-6855; Mohanty, Sujata/0000-0002-0047-4914; Nieuwland, Rienk/0000-0002-5671-3400; Ochiya, Takahiro/0000-0002-0776-9918; Sahoo, Susmita/0000-0002-7279-1564; Torrecilhas, Ana C./0000-0001-5724-2199; Zheng, Lei/0000-0003-2576-8780; Zijlstra, Andries/0000-0001-8460-8803; Brucale, Marco/0000-0001-7244-4389; Carney, Randy P./0000-0001-8193-1664; Crescitelli, Rossella/0000-0002-1714-3169; Haughey, Norman J./0000-0001-5194-4122; Martens‐Uzunova, Elena S./0000-0002-5363-2525; Newman, Lauren A./0000-0003-3303-1666; Rohde, Eva/0000-0001-8692-886X; Sandau, Ursula S./0000-0002-3646-7089; Saugstad, Julie A./0000-0002-2996-9611; Shekari, Faezeh/0000-0001-6026-5412; Tosar, Juan P./0000-0002-2021-2479; Varga, Zoltán/0000-0002-5741-2669; Wauben, Marca H. M./0000-0003-0360-0311; Wehman, Ann M./0000-0001-9826-4132; Zimmerman, Alan J./0000-0001-6280-4790; Théry, Clotilde/0000-0001-8294-6884; Witwer, Kenneth W./0000-0003-1664-4233; Bodnár, Bernadett Réka/0000-0003-3347-9225; Bukva, Mátyás/0000-0002-5225-0285; Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Dobra, Gabriella/0000-0002-2814-7720; Försönits, András/0000-0002-9298-8890; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Márton Ákos/0000-0002-2819-5116; Németh, Krisztina/0000-0002-3825-2137; Oláh, Attila/0000-0003-4122-5639; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Stepanova, Ganna/0000-0002-8285-2762; Visnovitz, Tamás/0000-0002-7962-5083; Wiener, Zoltán/0000-0001-7056-4926; Harmati, Mária/0000-0002-4875-5723; Hegyesi, Hargita/0000-0002-8800-5169}
}

@article{MTMT:34523817,
	title = {Tumour regression predicts better response to interferon therapy in melanoma patients. a retrospective single centre study.},
	url = {https://m2.mtmt.hu/api/publication/34523817},
	author = {Mezőlaki, Noémi and Baltás, Eszter and Ócsai, Henriette and Varga, Anita and Korom, Irma and Varga, Erika and Németh, István Balázs and Kis, Erika and Varga, János and Kocsis, Ádám László and Gyulai, Rolland Péter and Bukva, Mátyás and Kemény, Lajos and Oláh, Judit Magdolna},
	doi = {10.1097/CMR.0000000000000935},
	journal-iso = {MELANOMA RES},
	journal = {MELANOMA RESEARCH},
	volume = {34},
	unique-id = {34523817},
	issn = {0960-8931},
	abstract = {We hypothesise that regression may have an impact on the effectiveness of adjuvant IFN therapy, based on its role in the host immune response. Our purpose is to investigate regression and ulceration as prognostic factors in case of interferon-alpha (IFN)-treated melanoma patients. We followed 357 IFN-treated melanoma patients retrospectively, investigating progression-free survival (PFS) and overall survival (OS) depending on the presence of ulceration and regression. A Kaplan-Meier analysis was performed, and we used a Cox regression analysis to relate risk factors. The survival function of the Cox regression was used to measure the effect of regression and ulceration on PFS and OS depending on the Breslow thickness (T1-T4) of the primary tumour. Regression was significantly positively related to PFS ( P  = 0.0018, HR = 0.352) and OS ( P  = 0.0112, HR = 0.380), while ulceration showed a negative effect (PFS: P  = 0.0001, HR = 2.629; OS: P  = 0.0003, HR = 2.388). They influence survival independently. The most favourable outcome was measured in the regressed/non-ulcerated group, whereas the worse was in the non-regressed/ulcerated one. Of risk factors, Breslow thickness is the most significant predictor. The efficacy of regression is regardless of Breslow thickness, though the more favourable the impact of regression was in the thicker primary lesions. Our results indicate that regression is associated with a more favourable outcome for IFN-treated melanoma patients, whereas ulceration shows an inverse relation. Further studies are needed to analyse the survival benefit of regression in relation to innovative immune checkpoint inhibitors.},
	year = {2024},
	eissn = {1473-5636},
	pages = {54-62},
	orcid-numbers = {Baltás, Eszter/0000-0003-0357-7393; Gyulai, Rolland Péter/0000-0002-3286-8846; Bukva, Mátyás/0000-0002-5225-0285; Kemény, Lajos/0000-0002-2119-9501}
}

@article{MTMT:34417027,
	title = {Machine learning-based analysis of cancer cell-derived vesicular proteins revealed significant tumor-specificity and predictive potential of extracellular vesicles for cell invasion and proliferation - A meta-analysis.},
	url = {https://m2.mtmt.hu/api/publication/34417027},
	author = {Bukva, Mátyás and Dobra, Gabriella and Gyukity-Sebestyén, Edina and Böröczky, Timea and Korsós, Marietta Margaréta and David G, Meckes and Horváth, Péter and Buzás, Krisztina and Harmati, Mária},
	doi = {10.1186/s12964-023-01344-5},
	journal-iso = {CELL COMM SIGN},
	journal = {CELL COMMUNICATION AND SIGNALING},
	volume = {21},
	unique-id = {34417027},
	issn = {1478-811X},
	abstract = {Although interest in the role of extracellular vesicles (EV) in oncology is growing, not all potential aspects have been investigated. In this meta-analysis, data regarding (i) the EV proteome and (ii) the invasion and proliferation capacity of the NCI-60 tumor cell lines (60 cell lines from nine different tumor types) were analyzed using machine learning methods.On the basis of the entire proteome or the proteins shared by all EV samples, 60 cell lines were classified into the nine tumor types using multiple logistic regression. Then, utilizing the Least Absolute Shrinkage and Selection Operator, we constructed a discriminative protein panel, upon which the samples were reclassified and pathway analyses were performed. These panels were validated using clinical data (n = 4,665) from Human Protein Atlas.Classification models based on the entire proteome, shared proteins, and discriminative protein panel were able to distinguish the nine tumor types with 49.15%, 69.10%, and 91.68% accuracy, respectively. Invasion and proliferation capacity of the 60 cell lines were predicted with R2 = 0.68 and R2 = 0.62 (p < 0.0001). The results of the Reactome pathway analysis of the discriminative protein panel suggest that the molecular content of EVs might be indicative of tumor-specific biological processes.Integrating in vitro EV proteomic data, cell physiological characteristics, and clinical data of various tumor types illuminates the diagnostic, prognostic, and therapeutic potential of EVs. Video Abstract.},
	keywords = {CLASSIFICATION; PROLIFERATION; INVASION; PREDICTION; machine learning; Extracellular vesicles; NCI-60},
	year = {2023},
	eissn = {1478-811X},
	orcid-numbers = {Bukva, Mátyás/0000-0002-5225-0285; Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Böröczky, Timea/0009-0009-3390-7809; Buzás, Krisztina/0000-0001-8933-2033; Harmati, Mária/0000-0002-4875-5723}
}

@article{MTMT:34316032,
	title = {Effect of sodium-glucose co-transporter inhibitors on arterial stiffness in patients with type 2 diabetes mellitus},
	url = {https://m2.mtmt.hu/api/publication/34316032},
	author = {Szigeti, J and Bukva, Mátyás and Gaszner, Balázs},
	doi = {10.1093/eurheartj/ehad655.2567},
	journal-iso = {EUR HEART J},
	journal = {EUROPEAN HEART JOURNAL},
	volume = {44},
	unique-id = {34316032},
	issn = {0195-668X},
	year = {2023},
	eissn = {1522-9645},
	orcid-numbers = {Bukva, Mátyás/0000-0002-5225-0285}
}

@article{MTMT:34231255,
	title = {Extracellular vesicle-mediated intercellular communication in cancer},
	url = {https://m2.mtmt.hu/api/publication/34231255},
	author = {Harmati, Mária and Bukva, Mátyás and Dobra, Gabriella and Gyukity-Sebestyén, Edina and Böröczky, Timea and Szabó, Zoltán and Kónya, Zoltán and Horvath, Peter and Klekner, Almos and Buzás, Krisztina},
	journal-iso = {EUR J IMMUNOL},
	journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
	volume = {53},
	unique-id = {34231255},
	issn = {0014-2980},
	year = {2023},
	eissn = {1521-4141},
	pages = {39-40},
	orcid-numbers = {Harmati, Mária/0000-0002-4875-5723; Bukva, Mátyás/0000-0002-5225-0285; Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Böröczky, Timea/0009-0009-3390-7809; Szabó, Zoltán/0000-0001-8278-8038; Kónya, Zoltán/0000-0002-9406-8596; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:34028861,
	title = {Diagnosis of Epstein-Barr and cytomegalovirus infections using decision trees: an effective way to avoid antibiotic overuse in paediatric tonsillopharyngitis},
	url = {https://m2.mtmt.hu/api/publication/34028861},
	author = {Takács, Andrea Tímea and Bukva, Mátyás and Bereczki, Csaba and Burián, Katalin and Terhes, Gabriella},
	doi = {10.1186/s12887-023-04103-0},
	journal-iso = {BMC PEDIATR},
	journal = {BMC PEDIATRICS},
	volume = {23},
	unique-id = {34028861},
	issn = {1471-2431},
	year = {2023},
	eissn = {1471-2431},
	orcid-numbers = {Takács, Andrea Tímea/0000-0001-9211-6593; Bukva, Mátyás/0000-0002-5225-0285; Bereczki, Csaba/0000-0003-0091-3558; Burián, Katalin/0000-0003-1300-2374; Terhes, Gabriella/0000-0002-7301-9672}
}

@CONFERENCE{MTMT:33871443,
	title = {Low birth weight associated with severe COVID-19 in children older than one year},
	url = {https://m2.mtmt.hu/api/publication/33871443},
	author = {Csizek, Zsófia and Bukva, Mátyás and Cintia, Petes and Szabó, Attila and Kiss, Tamás},
	booktitle = {31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023)},
	unique-id = {33871443},
	year = {2023},
	pages = {42-42},
	orcid-numbers = {Szabó, Attila/0000-0001-7321-9861; Kiss, Tamás/0000-0001-5339-5227}
}

@article{MTMT:33592853,
	title = {MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles},
	url = {https://m2.mtmt.hu/api/publication/33592853},
	author = {Dobra, Gabriella and Gyukity-Sebestyén, Edina and Bukva, Mátyás and Harmati, Mária and Nagy, Valentina and Szabó, Zoltán and Pankotai, Tibor and Klekner, Álmos and Buzás, Krisztina},
	doi = {10.3390/cancers15030712},
	journal-iso = {CANCERS},
	journal = {CANCERS},
	volume = {15},
	unique-id = {33592853},
	abstract = {Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood–brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)—which can cross the BBB and are stable in body fluids—to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch’s test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours.},
	year = {2023},
	eissn = {2072-6694},
	orcid-numbers = {Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Bukva, Mátyás/0000-0002-5225-0285; Harmati, Mária/0000-0002-4875-5723; Szabó, Zoltán/0000-0001-8278-8038; Pankotai, Tibor/0000-0001-9810-5465; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:33554742,
	title = {Impact of Experimental Conditions on Extracellular Vesicles’ Proteome: A Comparative Study},
	url = {https://m2.mtmt.hu/api/publication/33554742},
	author = {Böröczky, Timea and Dobra, Gabriella and Bukva, Mátyás and Gyukity-Sebestyén, Edina and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Horváth, Péter and Buzás, Krisztina and Harmati, Mária},
	doi = {10.3390/life13010206},
	journal-iso = {LIFE-BASEL},
	journal = {LIFE-BASEL},
	volume = {13},
	unique-id = {33554742},
	abstract = {Extracellular vesicle (EV) research is a rapidly developing field, mainly due to the key role of EVs in intercellular communication and pathophysiological processes. However, the heterogeneity of EVs challenges their exploration and the establishment of gold-standard methods. Here, we aimed to reveal the influence of technical changes on EV biology and the reliability of experimental data. We used B16F1 melanoma cells as a model and applied nanoparticle tracking analysis, mass spectrometry (LC-MS/MS) and pathway enrichment analysis to analyze the quantity, size distribution, proteome and function of their small EVs (sEVs) produced in sEV-depleted fetal bovine serum (FBS)-containing medium or serum-free medium. Additionally, we investigated the effects of minor technical variances on the quality of sEV preparations. We found that storage of the isolates at −80 °C has no adverse effect on LC-MS/MS analysis, and an additional washing step after differential ultracentrifugation has a minor influence on the sEV proteome. In contrast, FBS starvation affects the production and proteome of sEVs; moreover, these vesicles may have a greater impact on protein metabolism, but a smaller impact on cell adhesion and membrane raft assembly, than the control sEVs. As we demonstrated that FBS starvation has a strong influence on sEV biology, applying serum-free conditions might be considered in in vitro sEV studies.},
	year = {2023},
	eissn = {2075-1729},
	orcid-numbers = {Böröczky, Timea/0009-0009-3390-7809; Dobra, Gabriella/0000-0002-2814-7720; Bukva, Mátyás/0000-0002-5225-0285; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Buzás, Krisztina/0000-0001-8933-2033; Harmati, Mária/0000-0002-4875-5723}
}

@article{MTMT:33314398,
	title = {Epidemiology and clinical features of SARS-CoV-2 infection in hospitalized children across four waves in Hungary: A retrospective, comparative study from March 2020 to December 2021},
	url = {https://m2.mtmt.hu/api/publication/33314398},
	author = {Takács, Andrea Tímea and Bukva, Mátyás and Gavaller, Gabriella and Kapus, Katalin and Rozsa, Maria and Ban-Gagyi, Boglarka and Sinko, Maria and Szűcs, Dániel and Terhes, Gabriella and Bereczki, Csaba},
	doi = {10.1002/hsr2.937},
	journal-iso = {HEAL SCI REP},
	journal = {HEALTH SCIENCE REPORTS},
	volume = {5},
	unique-id = {33314398},
	abstract = {Background and AimsFrom 2019 till the present, infections induced by the novel coronavirus and its mutations have posed a new challenge for healthcare. However, comparative studies on pediatric infections throughout waves are few. During four different pandemic waves, we intended to investigate the clinical and epidemiological characteristic of the pediatric population hospitalized for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus infection. MethodsBetween March 2020 and December 2021, we performed our retrospective research on children infected with the SARS-CoV-2 virus at the University of Szeged. We analyzed the data of all patients who required hospitalization due to positive results of SARS-CoV-2 tests (Nucleic Acid Amplification Test or rapid antigen test). Data analysis included demographic data, medical history, clinical findings, length of hospitalization, and complications, using medical records. ResultsIn this study, data from 358 coronavirus-infected children were analyzed. The most affected age group was children over 1 month and under 1 year (30.2%). The highest number of cases was recorded in the fourth wave (53.6%). Fever (65.6%), cough (51.4%), nasal discharge (35.3%), nausea and vomiting (31.3%), and decreased oral intake (28.9%) were the most common symptoms. The most common complications were dehydration (50.5%), pneumonia (14.9%), and bronchitis/bronchiolitis (14.5%). Based on RR values, there are considerable differences in the prevalence of the symptoms and complications between the different age groups and waves. Cox proportional hazard model analyzes showed that fever and tachypnoea had a relevant effect on days to recovery. ConclusionsWe found trends similar to those previously published, overall statistics. The proportion of children requiring hospitalization varied from wave to wave, with the fourth wave affecting the Hungarian child population the most. Our findings suggest that hospitalization time is unrelated to age, but that certain symptoms (fever and tachypnoea) are associated with longer hospitalization. The onset of certain symptoms may differ by age group.},
	keywords = {CHILDREN; Public, Environmental & Occupational Health; COVID-19; hospitalization wave},
	year = {2022},
	eissn = {2398-8835},
	orcid-numbers = {Takács, Andrea Tímea/0000-0001-9211-6593; Bukva, Mátyás/0000-0002-5225-0285; Terhes, Gabriella/0000-0002-7301-9672; Bereczki, Csaba/0000-0003-0091-3558}
}