TY - JOUR AU - Balázs, Márta AU - Péter, Izabella AU - Bartos, Hunor AU - Bodor, Zsolt AU - Antal, Emőke AU - Albert, Csilla AU - Miklóssy, Ildikó TI - Organic Acid Production by Basfia succiniciproducens from Agro-Industrial By-Products. JF - BIOTECH J2 - BIOTECH VL - 14 PY - 2025 IS - 3 PG - 18 SN - 2673-6284 DO - 10.3390/biotech14030068 UR - https://m2.mtmt.hu/api/publication/36366345 ID - 36366345 N1 - Funding Agency and Grant Number: Department of Bioengineering, Sapientia Hungarian University of Transylvania; Doctoral School of Chemistry, University of Pecs Funding text: The authors declare that funding was provided by the Department of Bioengineering, Sapientia Hungarian University of Transylvania, and the work of Marta Balazs was supported by the Doctoral School of Chemistry, University of Pecs. AB - A continuous effort is needed to develop sustainable production methods for industrial platform chemicals. B. succiniciproducens, a natural succinic acid-producer, can metabolize five and six carbon atoms containing sugars in pure form as well as from agro-industrial wastes. In our work, we investigate the conversion of industrial by-products, apple pomace from apple juice production, and whey waste from milk processing to succinic acid and other organic acids (lactic, formic, and acetic acid). We obtained a succinic acid yield of 0.224 g/g total consumed fermentable sugars, lactic acid yield was 0.087 g/g, in turn, formic acid was produced at a 0.034 g/g yield, and acetic acid was obtained at 0.010 g/g total consumed fermentable sugars, using a thermal pretreated apple pomace-based medium. In the case of pretreated whey-based medium formulation, we obtained a succinic acid yield of 0.236 g/g consumed lactose, while formic acid and acetic acid were produced as well (0.09 g/g and 0.101 g/g, respectively). We demonstrate that lactose is a promising carbon source for organic acid production by B. succiniciproducens, while our study is the first to propose the use of a similarly available agro-industrial by-product, apple pomace, for the fermentative production of succinic acid by B. succiniciproducens. LA - English DB - MTMT ER - TY - JOUR AU - Sinkler, Réka AU - Balázs, Márta AU - Antal, Emőke AU - Bartos, Hunor AU - Lányi, Szabolcs AU - Miklóssy, Ildikó TI - METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS JF - STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA J2 - STUD UNIV BABES-BOLYAI CHEM VL - 64 PY - 2019 IS - 2 SP - 159 EP - 174 PG - 16 SN - 1224-7154 DO - 10.24193/subbchem.2019.2.13 UR - https://m2.mtmt.hu/api/publication/30765679 ID - 30765679 N1 - Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Gheorghe Polizu str, Bucharest, RO-011061, Romania University of Pécs, Faculty of Natural Sciences, Doctoral School of Chemistry, 6 Ifjúság str, Pécs, Hu-7624, Hungary Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Miercurea Ciuc, RO-530104, Romania Export Date: 4 November 2019 Correspondence Address: Miklóssy, I.; Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Romania; email: miklossyildiko@uni.sapientia.ro AB - 1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enzymes in a heterologous pathway leading to BDO production, which were introduced into a genome-engineered E. coli MG1655(DE3) Delta ldhA, Delta pflB strain. Knowing that the expression of recombinant proteins and gene deletions can significantly influence cellular viability, the present study was carried out to investigate the impact of the two key enzyme expression on deletion strains, helping us to analyze the physiological changes of E. coli strains and providing directions for further optimizations in order to achieve satisfying target product yields. LA - English DB - MTMT ER - TY - CONF AU - Balázs, Márta AU - Antal, Emőke AU - Salamon, Pál Attila AU - Miklóssy, Ildikó AU - Bodor, Zsolt ED - Majdik, Cornelia TI - Analysis of Basfia Succiniciproducens Gene Expression Profile by RT-qPCR Method T2 - 25th International Conference on Chemistry = XXV. Nemzetközi Vegyészkonferencia PB - Erdélyi Magyar Műszaki Tudományos Társaság (EMT) C1 - Kolozsvár T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 PY - 2019 SP - 31384216 UR - https://m2.mtmt.hu/api/publication/31365300 ID - 31365300 LA - English DB - MTMT ER - TY - CHAP AU - Antal, Emőke AU - Salamon, Pál Attila AU - Miklóssy, Ildikó TI - In silico examination of a malonyl-CoA-reductase enzyme for metabolic engineering purposes T2 - ABSTRACTS BOOK, 19th International Symposium and Summer School on Bioanalysis, 2019 PB - Babes-Bolyai University CY - Cluj SN - 9789730298987 PY - 2019 SP - 31365350 UR - https://m2.mtmt.hu/api/publication/31371984 ID - 31371984 LA - English DB - MTMT ER - TY - CHAP AU - Antal, Emőke AU - Miklóssy, Ildikó ED - Garay, Tamás ED - Gáspári, Zoltán TI - Anyagcseremérnökségben alkalmazható malonil-KoA-reduktáz expressziója és jellemzése T2 - XXXIV. Országos Tudományos Diákköri Konferencia: Biológia Szekció PB - Pázmány Péter Katolikus Egyetem Információs Technológiai és Bionikai Kar CY - Budapest SN - 9789633083550 PY - 2019 SP - 87 UR - https://m2.mtmt.hu/api/publication/31372114 ID - 31372114 LA - Hungarian DB - MTMT ER - TY - CONF AU - Antal, Emőke AU - Balázs, Márta AU - Bodor, Zsolt AU - Miklóssy, Ildikó ED - Majdik, Cornelia TI - In silico characterization of a malonyl-CoA-reductase enzyme T2 - 25th International Conference on Chemistry = XXV. Nemzetközi Vegyészkonferencia PB - Erdélyi Magyar Műszaki Tudományos Társaság (EMT) C1 - Kolozsvár T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 PY - 2019 SP - 31372079 UR - https://m2.mtmt.hu/api/publication/31386219 ID - 31386219 LA - English DB - MTMT ER - TY - CONF AU - Antal, Emőke AU - Balázs, Márta AU - Sinkler, Réka AU - Bodor, Zsolt AU - Miklóssy, Ildikó ED - Majdik, Cornelia TI - Studies of dehydrogenases applicable in metabolic engineering T2 - XXIV. Nemzetközi Vegyészkonferencia PB - Erdélyi Magyar Műszaki Tudományos Társaság (EMT) T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 PY - 2018 SP - 30373698 UR - https://m2.mtmt.hu/api/publication/31372092 ID - 31372092 LA - Hungarian DB - MTMT ER - TY - THES AU - Antal, Emőke TI - CRISPR/Cas9 génszerkesztési technológia PY - 2018 UR - https://m2.mtmt.hu/api/publication/31372160 ID - 31372160 LA - Hungarian DB - MTMT ER - TY - THES AU - Antal, Emőke TI - Anyagcseremérnökségben alkalmazható malonil-KoA-reduktáz expressziója és jellemzése PY - 2018 UR - https://m2.mtmt.hu/api/publication/31372343 ID - 31372343 LA - Hungarian DB - MTMT ER - TY - CONF AU - Balázs, Márta AU - Bartos, Hunor AU - Antal, Emőke AU - Miklóssy, Ildikó AU - Bodor, Zsolt ED - Majdik, Cornelia TI - A Basfia succiniciproducens bioszintetikus potenciáljának tanulmányozása T2 - XXIV. Nemzetközi Vegyészkonferencia PB - Erdélyi Magyar Műszaki Tudományos Társaság (EMT) T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 PY - 2018 SP - 3284931 UR - https://m2.mtmt.hu/api/publication/31386269 ID - 31386269 LA - Hungarian DB - MTMT ER -