TY - THES AU - Manteghi, Reihaneh TI - Strategies for development of antimicrobial peptides and proteins PB - Szegedi Tudományegyetem PY - 2022 SP - 51 DO - 10.14232/phd.11284 UR - https://m2.mtmt.hu/api/publication/34117484 ID - 34117484 AB - Many researches have been done with the aim of overcoming AMPs challenges. Different modification strategies as well as different delivery development ideas were offered by these researches. However, peptide modifications and formulation of peptide delivery systems are challenging tasks and hide several risks. Understanding and evaluating the cause - effect relations within the initial Risk Assessment (RA) step in case of all attributes is novelty since it gives the basis for the experimental design as the next step, and aids the formulation development in order to get the final product in the targeted quality range. It also helps to focus on the resources (human, financial, time) related to the final product quality aimed at. By means of RA method within QbD approach of early pharmaceutical development we monitored the factors with highly risk potential in the PEGylation process and risks such as loosing antimicrobial activity of peptide are prevented. The selection of CQAs, CQAs, QTPPs, CQAs and CPPs/CMAs of a PEGylated PGLa formula was performed and interdependence rating among the QTPP elements and CQAs, as well as among the CPPs and CQAs was performed. This careful theoretical study led to the selection of the right methodologies and materials in the synthesis of PEGylated AMPs and their formulation and consequently resulted in obtaining optimized formulation. In our second work, LYZ encapsulated in a novel polyelectrolyte core-shell nanoparticles through the LBL technique utilized as a carrier system to control the release of protein. The preparation of LYZ NPs was made according to 23 full factorial design with QbD approach. Our aim was to understand the effect of process parameters through the determination of mathematical equations, based on which the optimization parameters can be predicted under different process parameters. The optimization parameters were encapsulation efficiency, particle size, enzyme activity, and the amount of α-helix structure. The nanoparticles were analyzed with transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and circular dichroism (CD) spectroscopy. Based on our results, we found that pH was the most important factor and pH 10 was recommended during the formulation. Enzyme activity and α-helix content correlated with each other very well, and particle size and encapsulation efficiency also showed a very good correlation with each other. The results of the α-helix content of FTIR and CD measurements were very similar for the precipitated lysozyme due to the solid-state of lysozyme. LA - English DB - MTMT ER - TY - JOUR AU - Manteghi, Reihaneh AU - Kristó, Katalin AU - Szakonyi, Gerda AU - Pannonhalminé Csóka, Ildikó TI - Recent insight into strategies for the design of antimicrobial peptides (AMPs) JF - ACTA PHARMACEUTICA HUNGARICA J2 - ACTA PHARM HUNG VL - 92 PY - 2022 IS - 1 SP - 20 EP - 37 PG - 18 SN - 0001-6659 DO - 10.33892/aph.2022.92.20-37 UR - https://m2.mtmt.hu/api/publication/32793624 ID - 32793624 LA - English DB - MTMT ER - TY - CHAP AU - Manteghi, Reihaneh AU - Kristó, Katalin AU - Pannonhalminé Csóka, Ildikó ED - Uhljar, Luca Éva ED - Sipos, Bence TI - Optimization of layering technique and the secondary structure analysis during formulation of nanoparticles containing lysozyme by Quality by Design approach T2 - IV. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science : book of abstracts PB - University of Szeged CY - Szeged PY - 2022 SP - 34 EP - 34 PG - 1 DO - 10.14232/syrptbrs.2022.34 UR - https://m2.mtmt.hu/api/publication/32639674 ID - 32639674 LA - English DB - MTMT ER - TY - CHAP AU - Manteghi, Reihaneh AU - Kristó, Katalin AU - Szakonyi, Gerda AU - Csóka, Ildikó ED - Uhljar, Luca Éva TI - Strategies for development of antimicrobial peptides and proteins T2 - III. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science PB - Szegedi Tudományegyetem (SZTE) CY - Szeged PY - 2021 SP - 20 EP - 20 PG - 1 DO - 10.14232/syrptbrs.2021.op8 UR - https://m2.mtmt.hu/api/publication/32640325 ID - 32640325 LA - English DB - MTMT ER - TY - JOUR AU - Kristó, Katalin AU - Manteghi, Reihaneh AU - Ibrahim, Yousif AU - Ungor, Ditta Anita AU - Csapó, Edit AU - Berkesi, Dániel Simon AU - Kónya, Zoltán AU - Pannonhalminé Csóka, Ildikó TI - Optimization of layering technique and secondary structure analysis during the formulation of nanoparticles containing lysozyme by quality by design approach JF - PLOS ONE J2 - PLOS ONE VL - 16 PY - 2021 IS - 12 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0260603 UR - https://m2.mtmt.hu/api/publication/32531578 ID - 32531578 N1 - Cited By :1 Export Date: 15 August 2023 CODEN: POLNC AB - In our study, core-shell nanoparticles containing lysozyme were formulated with precipitation and layering self-assembly. Factorial design (DoE) was applied by setting the process parameters during the preparation with Quality by Design (QbD) approach. The factors were the concentration of lysozyme and sodium alginate, and pH. Our aim was to understand the effect of process parameters through the determination of mathematical equations, based on which the optimization parameters can be predicted under different process parameters. The optimization parameters were encapsulation efficiency, particle size, enzyme activity and the amount of α-helix structure. The nanoparticles were analysed with transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR) and circular dichroism (CD) spectroscopy. Based on our results, we found that pH was the most important factor and pH 10 was recommended during the formulation. Enzyme activity and α-helix content correlated with each other very well, and particle size and encapsulation efficiency also showed very good correlation with each other. The results of the α-helix content of FTIR and CD measurements were very similar for the precipitated lysozyme due to the solid state of lysozyme. The mixing time had the best influence on the encapsulation efficiency and the particle size, which leads to the conclusion that a mixing time of 1 h is recommended. The novelty in our study is the presentation of a mathematical model with which the secondary structure of the protein and other optimization parameters can be controlled in the future during development of nanoparticle based on the process parameters. LA - English DB - MTMT ER - TY - CONF AU - Manteghi, Reihaneh AU - Kristó, Katalin AU - Szakonyi, Gerda AU - Pannonhalminé Csóka, Ildikó ED - Benkő, Ernő ED - Regdon, Géza (ifj.) TI - PEGylation and formulation strategies of antimicrobial peptides and proteins development T2 - III. Fiatal Technológusok Fóruma PB - Szegedi Tudományegyetem (SZTE) C1 - Szeged PY - 2020 SP - 15 EP - 15 PG - 1 DO - 10.14232/ftf.2020.op10 UR - https://m2.mtmt.hu/api/publication/31781555 ID - 31781555 LA - English DB - MTMT ER - TY - CONF AU - Manteghi, Reihaneh AU - Szakonyi, Gerda AU - Pannonhalminé Csóka, Ildikó ED - Bíró, Tivadar TI - PEGylation and formulation strategies of antimicrobial peptides and proteins development T2 - II. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science PB - University of Szeged C1 - Szeged PY - 2020 SP - 42 EP - 42 PG - 1 UR - https://m2.mtmt.hu/api/publication/31361067 ID - 31361067 LA - English DB - MTMT ER - TY - JOUR AU - Manteghi, Reihaneh AU - Pallagi, Edina AU - Olajos, Gábor AU - Pannonhalminé Csóka, Ildikó TI - Pegylation and formulation strategy of Anti-Microbial Peptide (AMP) according to the quality by design approach JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 144 PY - 2020 PG - 9 SN - 0928-0987 DO - 10.1016/j.ejps.2019.105197 UR - https://m2.mtmt.hu/api/publication/31042655 ID - 31042655 N1 - DOI: 10.1016/j.ejps.2019.105197 LA - English DB - MTMT ER - TY - CONF AU - Pannonhalminé Csóka, Ildikó AU - Katona, Gábor AU - Dobó, Dorina Gabriella AU - Ismail, Ruba AU - Manteghi, Reihaneh AU - Pallagi, Edina TI - Potentials and threats of the colloidal systems as carriers for peptide drugs T2 - Eufeps Annual Meeting 2019 : Personalized medicines, targeted therapies, precision drug delivery PY - 2019 SP - 5 UR - https://m2.mtmt.hu/api/publication/30656699 ID - 30656699 LA - English DB - MTMT ER - TY - JOUR AU - Manteghi, Reihaneh AU - Szakonyi, Gerda AU - Pallagi, Edina AU - Pannonhalminé Csóka, Ildikó TI - Formulation strategy of anti-microbial peptide (AMP) delivery systems JF - ACTA PHARMACEUTICA HUNGARICA J2 - ACTA PHARM HUNG VL - 88 PY - 2018 IS - 3 SP - 192 EP - 192 PG - 1 SN - 0001-6659 UR - https://m2.mtmt.hu/api/publication/30656319 ID - 30656319 LA - English DB - MTMT ER -