TY - JOUR AU - Lakatos, Renáta Krisztina AU - Dobolyi, Árpád AU - Kovács, Zsolt TI - Uric acid and allopurinol aggravate absence epileptic activity in Wistar Albino Glaxo Rijswijk rats JF - BRAIN RESEARCH J2 - BRAIN RES VL - 1686 PY - 2018 SP - 1 EP - 9 PG - 9 SN - 0006-8993 DO - 10.1016/j.brainres.2018.02.012 UR - https://m2.mtmt.hu/api/publication/3339535 ID - 3339535 N1 - Institute of Biology, University of Pécs, Pécs, Hungary Savaria Department of Biology, Savaria University Centre, ELTE Eötvös Loránd University, Szombathely, Hungary Laboratory of Neuromorphology and Human Brain Tissue Bank, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Hungarian Academy of Sciences and Eötvös Loránd University, Budapest, Hungary Cited By :3 Export Date: 22 June 2023 CODEN: BRREA Correspondence Address: Kovács, Z.; Savaria Department of Biology, Hungary; email: kovacs.zsolt@sek.elte.hu LA - English DB - MTMT ER - TY - CONF AU - Lakatos, Renáta Krisztina AU - Dobolyi, A AU - Kekesi, KA AU - Aleksza, M AU - Kovács, Zsolt TI - Guanosine may increase absence epileptic activity in Wistar Albino Glaxo Rijswijk rats T2 - 5th FENS Regional Meeting 2017 PB - Federation of European Neuroscience Societies C1 - Pécs PY - 2017 SP - 266 UR - https://m2.mtmt.hu/api/publication/3314738 ID - 3314738 N1 - Poszter LA - English DB - MTMT ER - TY - CONF AU - Lakatos, Renáta Krisztina AU - Kovács, Zsolt ED - Puskás, János TI - A guanozin hatása az abszensz epilepsziás aktivitásra WAG/Rij patkányban T2 - XII. Regionális Természettudományi Konferencia C1 - Szombathely PY - 2017 SP - 12 EP - 12 PG - 1 UR - https://m2.mtmt.hu/api/publication/3180977 ID - 3180977 N1 - Előadás AB - XII. Regionális Természettudományi Konferencia 2017. január 25.; Az előadások összefoglalói; Szerkesztő: Dr. Puskás János LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Lakatos, Renáta Krisztina AU - Barna, János AU - Dobolyi, Árpád TI - Absence epileptic activity in Wistar Albino Glaxo Rijswijk rat mothers JF - BRAIN RESEARCH J2 - BRAIN RES VL - 1657 PY - 2017 SP - 368 EP - 376 PG - 9 SN - 0006-8993 DO - 10.1016/j.brainres.2017.01.005 UR - https://m2.mtmt.hu/api/publication/3163629 ID - 3163629 N1 - Funding Agency and Grant Number: National Development Agency of Hungary [TIOP-1.3.1.-07/2-2F-2009-2008]; KTIA NAP-B Research Program [KTIA NAP_B_13-2-2014-0004]; NKFIHNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA_K116538, NVKP_16, VEKOP-2.3-15] Funding text: This work was supported by the National Development Agency of Hungary [TIOP-1.3.1.-07/2-2F-2009-2008; Zsolt Kovacs]; the KTIA NAP-B Research Program [KTIA NAP_B_13-2-2014-0004; Arpad Dobolyi]; the NKFIH [OTKA_K116538 Research Grant; Arpad Dobolyi]; the NKFIH [NVKP_16 Grant; Arpad Dobolyi], and the NKFIH [VEKOP-2.3-15 Grant; Arpad Dobolyi]. We wish to thank Tamas Torok (NYME SEK) for the technical assistance. AB - Abstract Absence epileptic activity was analyzed during pregnancy, the postpartum period and after weaning to establish alterations of seizures throughout the reproductive cycle. Wistar Albino Glaxo Rijswijk (WAG/Rij) rats were used in the study as a model of absence epilepsy and because their seizures do not interfere with rearing offspring. The number of spike-wave discharges (SWDs) was gradually elevated from the 19th pregnancy day to delivery. Meanwhile, the characteristics of individual SWDs did not change suggesting that SWD generation remained the same. In the postpartum and postweaning periods, the number of SWDs was not increased in the absence of pups. However, returning the pups to mothers resulted in a markedly elevated number of SWDs for 1 h. If pups were taken away after 30 min, the number of SWDs dropped immediately suggesting that the presence of pups increased the SWD number. The time mothers spent with the litter and in kyphosis suckling posture were in correlation with their SWD number further suggesting the importance of interaction with pups in SWD induction. Suckling elevates prolactin levels but surprisingly, its intracerebroventricular injection markedly reduced SWD number in suckled WAG/Rij mothers suggesting that the SWD-inducing effect of suckling is not mediated by prolactin. Rather, the elevated prolactin level may provide some protection against pro-epileptic effects of suckling. In conclusion, we first identified periods within the reproductive cycle with increased absence epileptic activity, implying that more attention should be devoted to epileptic activity changes in mothers. LA - English DB - MTMT ER - TY - CONF AU - Lakatos, Renáta Krisztina AU - Dobolyi, Árpád AU - Kékesi, Adrienna Katalin AU - Juhász, Gábor Dénes AU - Kovács, Zsolt TI - Inosine, guanosine and uridine modulate the lipopolysaccharide-evoked changes in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats T2 - IBRO Workshop 2016 PY - 2016 SP - & UR - https://m2.mtmt.hu/api/publication/3064202 ID - 3064202 N1 - IBRO Workshop 2016, Budapest, 20-21 January 2016; P2/84; http://www.ibro2016.hu/images/downloads/IBRO_poster_sessions. pdf f LA - English DB - MTMT ER - TY - JOUR AU - Lakatos, Renáta Krisztina AU - Dobolyi, Árpád AU - Todorov, Mihail AU - Kékesi, Adrienna Katalin AU - Juhász, Gábor Dénes AU - Aleksza, Magdolna AU - Kovács, Zsolt TI - Guanosine may increase absence epileptic activity by means of A2A adenosine receptors in Wistar Albino Glaxo Rijswijk rats. JF - BRAIN RESEARCH BULLETIN J2 - BRAIN RES BULL VL - 124 PY - 2016 SP - 172 EP - 181 PG - 10 SN - 0361-9230 DO - 10.1016/j.brainresbull.2016.05.001 UR - https://m2.mtmt.hu/api/publication/3064172 ID - 3064172 N1 - Institute of Biology, University of Pécs, Ifjúság útja 6., Pécs, 7624, Hungary Department of Zoology, University of West Hungary Savaria Campus, Károlyi Gáspár tér 4., Szombathely, 9700, Hungary MTA-ELTE NAP B Laboratory of Molecular and Systems Neurobiology, Institute of Biology, Hungarian Academy of Sciences and Eötvös Loránd Univ., Pázmány Péter sétány 1C, Budapest, 1117, Hungary Laboratory of Neuromorphology and Human Brain Tissue Bank, Department of Anatomy, Histology and Embryology, Semmelweis University, Tuzoltó u. 58., Budapest, 1094, Hungary Laboratory of Proteomics, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary MTA-TTK NAP MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Magyar tudósok körútja 2., Budapest, 1117, Hungary Department of Botany, University of West Hungary Savaria Campus, Károlyi Gáspár tér 4., Szombathely, 9700, Hungary Cited By :10 Export Date: 25 October 2022 CODEN: BRBUD Correspondence Address: Kovács, Z.; Department of Zoology, Károlyi Gáspár tér 4., Hungary; email: zskovacs@ttk.nyme.hu AB - The non-adenosine nucleoside guanosine (Guo) was demonstrated to decrease quinolinic acid(QA)-induced seizures, spontaneously emerged absence epileptic seizures and lipopolysaccharide(LPS)-evoked induction of absence epileptic seizures suggesting its antiepileptic potential. It was also described previously that intraperitoneal (i.p.) injection of 20 and 50mg/kg Guo decreased the number of spike-wave discharges (SWDs) in a well investigated model of human absence epilepsy, the Wistar Albino Glaxo Rijswijk (WAG/Rij) rats during 4th (20mg/kg Guo) and 3rd as well as 4th (50mg/kg Guo) measuring hours. Guanosine can potentially decrease SWD number by means of its putative receptors but absence epileptic activity changing effects of Guo by means of increased extracellular adenosine (Ado) cannot be excluded. An increase in the dose of i.p. injected Guo is limited by its low solubility in saline, therefore, we addressed in the present study whether higher doses of Guo, diluted in sodium hydroxide (NaOH) solution, have more potent antiepileptic effect in WAG/Rij rats. We confirmed that i.p. 50mg/kg Guo decreased but, surprisingly, i.p. 100mg/kg Guo enhanced the number of SWDs in WAG/Rij rats. Combined i.p. injection of a non-selective Ado receptor antagonist theophylline (5mg/kg) or a selective Ado A2A receptor (A2AR) antagonist SCH 58261 (7-(2-phenylethyl)-5-amino-2-(2-furyl)-pyrazolo-[4,3-e]-1,2,4-triazolo[1,5-c]pyri midine) (1mg/kg) and a cyclooxygenase 1 and 2/COX-1 and COX-2 inhibitor indomethacin (10mg/kg) with 100mg/kg Guo decreased the SWD number compared to i.p. 100mg/kg Guo alone. The results suggest that i.p. 100mg/kg Guo can increase SWD number by means of the adenosinergic system. LA - English DB - MTMT ER - TY - CONF AU - Lakatos, Renáta Krisztina AU - Kovács, Zsolt ED - Puskás, János TI - A nem-adenozin nukleozidok hatása az LPS indukálta abszensz epilepsziás aktivitásra WAG/Rij patkányban T2 - XI. Regionális Természettudományi Konferencia PB - Nyugat-magyarországi Egyetem Savaria Egyetemi Központ C1 - Szombathely PY - 2016 SP - 11 PG - 1 UR - https://m2.mtmt.hu/api/publication/3006421 ID - 3006421 N1 - Előadás LA - Hungarian DB - MTMT ER - TY - CONF AU - Kovács, Zsolt AU - Katalin, A Kékesi AU - Árpád, Dobolyi AU - Lakatos, Renáta Krisztina AU - Gábor, Juhász TI - Inosine, guanosine and uridine change the absence epileptic activity in Wistar Albino Glaxo/Rijswijk rats T2 - XV. Biannual Conference of the Hungarian Neuroscience Society PY - 2015 SP - 61 EP - 61 PG - 1 UR - https://m2.mtmt.hu/api/publication/2920394 ID - 2920394 N1 - Poszter LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Kardos, Julianna AU - Kékesi, Adrienna Katalin AU - Juhász, Gábor Dénes AU - Lakatos, Renáta Krisztina AU - Héja, László TI - Effects of nucleosides on glia - Neuron interactions open up new vistas in the development of more effective antiepileptic drugs JF - CURRENT MEDICINAL CHEMISTRY J2 - CURR MED CHEM VL - 22 PY - 2015 IS - 12 SP - 1500 EP - 1514 PG - 15 SN - 0929-8673 DO - 10.2174/0929867322666150212153210 UR - https://m2.mtmt.hu/api/publication/2915179 ID - 2915179 N1 - Funding Agency and Grant Number: National Development Agency of Hungary [TIOP-1.3.1.-07/2-2F-2009-2008, TAMOP 4.2.1./B-09/1/KMR-2010-0003]; ERA-Chemistry [OTKA 102166]; [KMR_12-1-2012-0112 TRANSRAT] Funding text: This work was supported by the National Development Agency of Hungary (under Grant No. TIOP-1.3.1.-07/2-2F-2009-2008) (Zsolt Kovacs), National Development Agency of Hungary TAMOP 4.2.1./B-09/1/KMR-2010-0003 (Gabor Juhasz and Katalin Adrienna Kekesi), ERA-Chemistry OTKA 102166 and KMR_12-1-2012-0112 TRANSRAT. Department of Zoology, University of West Hungary, Savaria Campus, Károlyi Gáspár tér 4, Szombathely, 9700, Hungary Group of Functional Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Proteomics, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Cited By :2 Export Date: 6 April 2021 CODEN: CMCHE Correspondence Address: Kovács, Z.; Department of Zoology, Károlyi Gáspár tér 4, Hungary Department of Zoology, University of West Hungary, Savaria Campus, Károlyi Gáspár tér 4, Szombathely, 9700, Hungary Group of Functional Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Proteomics, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Cited By :2 Export Date: 7 April 2021 CODEN: CMCHE Correspondence Address: Kovács, Z.; Department of Zoology, Károlyi Gáspár tér 4, Hungary LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Kékesi, Adrienna Katalin AU - Dobolyi, Árpád AU - Lakatos, Renáta Krisztina AU - Juhász, Gábor Dénes TI - Absence epileptic activity changing effects of non-adenosine nucleoside inosine, guanosine and uridine in Wistar Albino Glaxo Rijswijk rats. JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 300 PY - 2015 SP - 593 EP - 608 PG - 16 SN - 0306-4522 DO - 10.1016/j.neuroscience.2015.05.054 UR - https://m2.mtmt.hu/api/publication/2904124 ID - 2904124 N1 - Department of Zoology, University of West Hungary, Savaria Campus, Karolyi Gaspár tér 4., Szombathely, 9700, Hungary Laboratory of Proteomics, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Department of Physiology and Neurobiology, Eötvös Loránd University, Pázmány Péter sétány 1C, Budapest, 1117, Hungary MTA-ELTE NAP Laboratory of Molecular and Systems Neurobiology, Institute of Biology, Hungarian Academy of Sciences and Eötvös Loránd Univ, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Laboratory of Neuromorphology and Human Brain Tissue Bank, Department of Anatomy, Histology and Embryology, Semmelweis University, Tuzoltó u. 58., Budapest, 1094, Hungary MTA-TTK NAP MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Cited By :29 Export Date: 22 June 2023 CODEN: NRSCD Correspondence Address: Kovács, Z.; Department of Zoology, Savaria Campus, Karolyi Gaspár tér 4., Hungary AB - Adenosine (Ado) and non-adenosine (non-Ado) nucleosides such as inosine (Ino), guanosine (Guo) and uridine (Urd) may have regionally different roles in the regulation of physiological and pathophysiological processes in the central nervous system (CNS) such as epilepsy. It was demonstrated previously that Ino and Guo decreased quinolinic acid (QA)-induced seizures and Urd reduced penicillin-, bicuculline- and pentylenetetrazole (PTZ)-induced seizures. It has also been demonstrated that Ino and Urd may exert their effects through GABAergic system by altering the function of GABAA type of gamma-aminobutyric acid receptors (GABAA receptors) whereas Guo decreases glutamate-induced excitability through glutamatergic system, which systems (GABAergic and glutamatergic) are involved in pathomechanisms of absence epilepsy. Thus, we hypothesized that Ino and Guo, similarly to the previously described effect of Urd, might also decrease absence epileptic activity. We investigated in the present study whether intraperitoneal (i.p.) application of Ino (500 and 1000mg/kg), Guo (20 and 50mg/kg), Urd (500 and 1000mg/kg), GABAA receptor agonist muscimol (1 and 3mg/kg), GABAA receptor antagonist bicuculline (2 and 4mg/kg), non-selective Ado receptor antagonist theophylline (5 and 10mg/kg) and non-competitive N-methyl-d-aspartate (NMDA) receptor antagonist (+)-5-methyl-10,11-dihydro-5H-dibenzo (a,d) cyclohepten-5,10-imine maleate (MK-801, 0.0625 and 0.1250mg/kg) alone and in combination have modulatory effects on absence epileptic activity in Wistar Albino Glaxo Rijswijk (WAG/Rij) rats. We found that Guo decreased the number of spike-wave discharges (SWDs) whereas Ino increased it dose-dependently. We strengthened that Urd can decrease absence epileptic activity. Our results suggest that Guo, Urd and their analogs could be potentially effective drugs for treatment of human absence epilepsy. LA - English DB - MTMT ER -