@article{MTMT:34720930,
	title = {A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)},
	url = {https://m2.mtmt.hu/api/publication/34720930},
	author = {Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter M and Bárkai, Tünde and Visnovitzné Dr Vukman, Krisztina and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey and Komlósi, Zsolt and Lőrincz, Péter and Valcz, Gábor and Buzás, Edit Irén},
	doi = {10.7554/eLife.95828.1},
	journal-iso = {ELIFE},
	journal = {ELIFE},
	volume = {13},
	unique-id = {34720930},
	issn = {2050-084X},
	year = {2024},
	eissn = {2050-084X},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Komlósi, Zsolt/0000-0002-4149-1497; Lőrincz, Péter/0000-0001-7374-667X; Valcz, Gábor/0000-0002-7109-3529; Buzás, Edit Irén/0000-0002-3744-206X}
}

@misc{MTMT:34692287,
	title = {A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)},
	url = {https://m2.mtmt.hu/api/publication/34692287},
	author = {Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter M and Bárkai, Tünde and Vukman, Krisztina V and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey and Komlósi, Zsolt I},
	unique-id = {34692287},
	year = {2024},
	pages = {2024},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394}
}

@article{MTMT:34567532,
	title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches},
	url = {https://m2.mtmt.hu/api/publication/34567532},
	author = {Welsh, Joshua A. and Goberdhan, Deborah C. I. and O'Driscoll, Lorraine and Buzás, Edit Irén and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Di Vizio, Dolores and Driedonks, Tom A. P. and Erdbrügger, Uta and Falcon‐Perez, Juan M. and Fu, Qing‐Ling and Hill, Andrew F. and Lenassi, Metka and Lim, Sai Kiang and Mahoney, Mỹ G. and Mohanty, Sujata and Möller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C. and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M. and Brucale, Marco and Burger, Dylan and Carney, Randy P. and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L. and Haughey, Norman J. and Hendrix, An and Ivanov, Alexander R. and Jovanovic‐Talisman, Tijana and Kruh‐Garcia, Nicole A. and Ku'ulei‐Lyn Faustino, Vroniqa and Kyburz, Diego and Lässer, Cecilia and Lennon, Kathleen M. and Lötvall, Jan and Maddox, Adam L. and Martens‐Uzunova, Elena S. and Mizenko, Rachel R. and Newman, Lauren A. and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S. and Saugstad, Julie A. and Shekari, Faezeh and Swift, Simon and Ter‐Ovanesyan, Dmitry and Tosar, Juan P. and Useckaite, Zivile and Valle, Francesco and Varga, Zoltán and van der Pol, Edwin and van Herwijnen, Martijn J. C. and Wauben, Marca H. M. and Wehman, Ann M. and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J. and Théry, Clotilde and Witwer, Kenneth W. and Beke-Somfai, Tamás and Szigyártó, Imola Csilla and Haseeb, Zubair},
	doi = {10.1002/jev2.12404},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {13},
	unique-id = {34567532},
	abstract = {Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.},
	year = {2024},
	eissn = {2001-3078},
	orcid-numbers = {Welsh, Joshua A./0000-0002-1097-9756; Goberdhan, Deborah C. I./0000-0003-0645-6714; Buzás, Edit Irén/0000-0002-3744-206X; Bussolati, Benedetta/0000-0002-3663-5134; Cai, Houjian/0000-0003-4887-2652; Falcon‐Perez, Juan M./0000-0003-3133-0670; Hill, Andrew F./0000-0001-5581-2354; Lenassi, Metka/0000-0002-9488-6855; Mohanty, Sujata/0000-0002-0047-4914; Nieuwland, Rienk/0000-0002-5671-3400; Ochiya, Takahiro/0000-0002-0776-9918; Sahoo, Susmita/0000-0002-7279-1564; Torrecilhas, Ana C./0000-0001-5724-2199; Zheng, Lei/0000-0003-2576-8780; Zijlstra, Andries/0000-0001-8460-8803; Brucale, Marco/0000-0001-7244-4389; Carney, Randy P./0000-0001-8193-1664; Crescitelli, Rossella/0000-0002-1714-3169; Haughey, Norman J./0000-0001-5194-4122; Martens‐Uzunova, Elena S./0000-0002-5363-2525; Newman, Lauren A./0000-0003-3303-1666; Rohde, Eva/0000-0001-8692-886X; Sandau, Ursula S./0000-0002-3646-7089; Saugstad, Julie A./0000-0002-2996-9611; Shekari, Faezeh/0000-0001-6026-5412; Tosar, Juan P./0000-0002-2021-2479; Varga, Zoltán/0000-0002-5741-2669; Wauben, Marca H. M./0000-0003-0360-0311; Wehman, Ann M./0000-0001-9826-4132; Zimmerman, Alan J./0000-0001-6280-4790; Théry, Clotilde/0000-0001-8294-6884; Witwer, Kenneth W./0000-0003-1664-4233; Bodnár, Bernadett Réka/0000-0003-3347-9225; Bukva, Mátyás/0000-0002-5225-0285; Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Dobra, Gabriella/0000-0002-2814-7720; Försönits, András/0000-0002-9298-8890; Ghosal, Sayam/0000-0001-6618-930X; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Márton Ákos/0000-0002-2819-5116; Németh, Krisztina/0000-0002-3825-2137; Oláh, Attila/0000-0003-4122-5639; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Stepanova, Ganna/0000-0002-8285-2762; Visnovitz, Tamás/0000-0002-7962-5083; Wiener, Zoltán/0000-0001-7056-4926; Harmati, Mária/0000-0002-4875-5723; Hegyesi, Hargita/0000-0002-8800-5169}
}

@article{MTMT:34425078,
	title = {Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/34425078},
	author = {Kovács, Kinga Dóra and Visnovitz, Tamás and Gerecsei, Tamás and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and Visnovitzné Dr Vukman, Krisztina and Balogh, Anna and Rajmon, Imola and Lőrincz, Péter and Székács, Inna and Buzás, Edit Irén and Horváth, Róbert},
	doi = {10.1002/jev2.12388},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {12},
	unique-id = {34425078},
	abstract = {In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level.},
	year = {2023},
	eissn = {2001-3078},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Lőrincz, Péter/0000-0001-7374-667X; Buzás, Edit Irén/0000-0002-3744-206X; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:33750753,
	title = {Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines},
	url = {https://m2.mtmt.hu/api/publication/33750753},
	author = {Koncz, Anna and Turiák, Lilla and Németh, Krisztina and Lenzinger, Dorina and Bárkai, Tünde and Lőrincz, Péter and Zelenyánszki, Helga and Visnovitzné Dr Vukman, Krisztina and Buzás, Edit Irén and Visnovitz, Tamás},
	doi = {10.3390/membranes13040431},
	journal-iso = {MEMBRANES-BASEL},
	journal = {MEMBRANES (BASEL)},
	volume = {13},
	unique-id = {33750753},
	abstract = {Cardiomyopathies are leading causes of human mortality. Recent data indicate that the cardiomyocyte-derived extracellular vesicles (EVs) released upon cardiac injury are present in circulation. This paper aimed to analyze EVs released under normal and hypoxic conditions by H9c2 (rat), AC16 (human) and HL1 (mouse) cardiac cell lines. Small (sEVs), medium (mEVs) and large EVs (lEVs) were separated from a conditioned medium by a combination of gravity filtration, differential centrifugation and tangential flow filtration. The EVs were characterized by microBCA, SPV lipid assay, nanoparticle tracking analysis, transmission and immunogold electron microscopy, flow cytometry and Western blotting. Proteomic profiles of the EVs were determined. Surprisingly, an endoplasmic reticulum chaperone, endoplasmin (ENPL, grp94 or gp96), was identified in the EV samples, and its association with EVs was validated. The secretion and uptake of ENPL was followed by confocal microscopy using GFP-ENPL fusion protein expressing HL1 cells. We identified ENPL as an internal cargo of cardiomyocyte-derived mEVs and sEVs. Based on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective role by reducing cardiomyocyte ER stress.},
	year = {2023},
	eissn = {2077-0375},
	orcid-numbers = {Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Lőrincz, Péter/0000-0001-7374-667X; Zelenyánszki, Helga/0000-0001-6768-3748; Buzás, Edit Irén/0000-0002-3744-206X; Visnovitz, Tamás/0000-0002-7962-5083}
}

@article{MTMT:33726492,
	title = {High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes},
	url = {https://m2.mtmt.hu/api/publication/33726492},
	author = {Németh, Krisztina and Tóth, Blanka and Sarnyai, Farkas and Koncz, Anna and Lenzinger, Dorina and Kereszturi, Éva and Visnovitz, Tamás and Kestecher, Brachyahu Meir and Osteikoetxea, Xabier and Csala, Miklós and Buzás, Edit Irén and Tamási, Viola},
	doi = {10.1186/s12986-023-00738-z},
	journal-iso = {NUTR METAB},
	journal = {NUTRITION & METABOLISM},
	volume = {20},
	unique-id = {33726492},
	issn = {1743-7075},
	year = {2023},
	eissn = {1743-7075},
	orcid-numbers = {Németh, Krisztina/0000-0002-3825-2137; Sarnyai, Farkas/0000-0002-5525-5508; Koncz, Anna/0000-0003-2511-2394; Visnovitz, Tamás/0000-0002-7962-5083; Osteikoetxea, Xabier/0000-0003-3628-0174; Csala, Miklós/0000-0002-3829-4361; Buzás, Edit Irén/0000-0002-3744-206X; Tamási, Viola/0000-0001-7419-5603}
}

@misc{MTMT:33589125,
	title = {Nanoinjection of fluorescent nanoparticles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/33589125},
	author = {Gerecsei, Tamás and Visnovitz, Tamás and Kovács, Kinga Dóra and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and Visnovitzné Dr Vukman, Krisztina and Lőrincz, Péter and Székács, Inna and Buzás, Edit Irén and Horváth, Róbert},
	unique-id = {33589125},
	year = {2022},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Péter/0000-0001-7374-667X; Buzás, Edit Irén/0000-0002-3744-206X; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:32735221,
	title = {Palmitoilált fluorszcens fehérjék sejten belüli és EV-kon történõ kifejezõdésének vizsgálata},
	url = {https://m2.mtmt.hu/api/publication/32735221},
	author = {Vizi, Péter Márk and Koncz, Anna and Visnovitz, Tamás},
	journal-iso = {ORVOSKÉPZÉS},
	journal = {ORVOSKÉPZÉS},
	volume = {97},
	unique-id = {32735221},
	issn = {0030-6037},
	year = {2022},
	pages = {302},
	orcid-numbers = {Koncz, Anna/0000-0003-2511-2394; Visnovitz, Tamás/0000-0002-7962-5083}
}

@article{MTMT:32550963,
	title = {Systematic transcriptomic and phenotypic characterization of human and murine cardiac myocyte cell lines and primary cardiomyocytes reveals serious limitations and low resemblances to adult cardiac phenotype},
	url = {https://m2.mtmt.hu/api/publication/32550963},
	author = {Onódi, Zsófia and Visnovitz, Tamás and Kiss, Bernadett and Hambalkó, Szabolcs and Koncz, Anna and Ágg, Bence and Váradi, Barnabás and Tóth, Viktória and Nagy, Regina Norma and Gergely, Tamás G and Gergő, Dorottya and Makkos, András and Pelyhe, Csilla and Varga, Nóra and Reé, Dóra and Apáti, Ágota and Leszek, Przemyslaw and Kovács, Tamás and Nagy, Nándor and Ferdinandy, Péter and Buzás, Edit Irén and Görbe, Anikó and Giricz, Zoltán and Varga, Zoltán},
	doi = {10.1016/j.yjmcc.2021.12.007},
	journal-iso = {J MOL CELL CARDIOL},
	journal = {JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY},
	volume = {165},
	unique-id = {32550963},
	issn = {0022-2828},
	year = {2022},
	eissn = {1095-8584},
	pages = {19-30},
	orcid-numbers = {Onódi, Zsófia/0000-0002-3746-8016; Visnovitz, Tamás/0000-0002-7962-5083; Kiss, Bernadett/0000-0001-7631-4418; Koncz, Anna/0000-0003-2511-2394; Ágg, Bence/0000-0002-6492-0426; Tóth, Viktória/0009-0003-9776-724X; Nagy, Regina Norma/0000-0002-3253-5398; Gergő, Dorottya/0000-0002-2726-3463; Makkos, András/0000-0002-0309-4909; Pelyhe, Csilla/0000-0001-6267-4527; Reé, Dóra/0000-0003-1967-1288; Kovács, Tamás/0000-0003-4127-4545; Nagy, Nándor/0000-0002-6223-5214; Ferdinandy, Péter/0000-0002-6424-6806; Buzás, Edit Irén/0000-0002-3744-206X; Görbe, Anikó/0000-0003-4908-1094; Giricz, Zoltán/0000-0003-2036-8665; Varga, Zoltán/0000-0002-2758-0784}
}

@misc{MTMT:32293810,
	title = {Hepatic extracellular vesicle release and uptake under normolipemia and hyperlipidemia},
	url = {https://m2.mtmt.hu/api/publication/32293810},
	author = {Németh, Krisztina and Zoltán, Varga and Lenzinger, Dorina and Visnovitz, Tamás and Koncz, Anna and Hegedüs, Nikolett and Ágnes, Kittel and Domokos, Máthé and Szigeti, Krisztián and Lőrincz, Péter and Edit, I. Buzás and Tamási, Viola},
	unique-id = {32293810},
	abstract = {Hepatic extracellular vesicle release and uptake under normolipemia and hyperlipidemia
		
		Krisztina Németh1, Zoltán Varga2,3, Dorina Lenzinger1, Tamás Visnovitz1, Anna Koncz1, Nikolett Hegedűs3, Ágnes Kittel4, Domokos Máthé3,5, Krisztián Szigeti3, Péter Lőrincz6, Edit I. Buzás1,7,8*, Viola Tamási1*
		
		1 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest
		2 Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest
		3 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
		4 Institute of Experimental Medicine, Eötvös Loránd Research Network, Budapest
		5 Hungarian Centre of Excellence for Molecular Medicine, In Vivo Imaging ACF, Budapest
		6 Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest
		7 Hungarian Centre of Excellence for Molecular Medicine – Semmelweis University Extracellular Vesicle Research Group, Budapest
		8 ELKH-SE Immune-Proteogenomics Research Group, Budapest
		
		Introduction Liver has a key role in removal of extracellular vesicles (EVs) from the blood circulation, and it also significantly contributes to EV secretion. However, the knowledge regarding to the involvement of the different liver cell types is scarce. 
		Aims Here we examined the different contribution of liver cell types in the dynamics of EV uptake and release both in normo- and hyperlipidemia.
		Methods Plasma EV profiles of C57BL/6 mice were analyzed after 20-30 weeks on high fat diet. Furthermore, control mice were injected intravenously with 99mTc-duramycin labelled medium (~ 330 nm) and small EVs (~ 130 nm), and an hour later, biodistribution of EVs were determined by SPECT/CT. In vitro, different liver cell types were tested for EV production and uptake with/without prior oleic acid/palmitic acid treatment. 
		Results Based on the EV marker expression, we found a significantly higher plasma small EV concentration after 30 weeks on high fat diet. To elucidate the involvement of primary hepatocytes, we carried out in vitro experiments. We report, that hyperlipidemic conditions significantly increased the release of medium EVs and small EVs. When investigating EV biodistribution, upon injection of 99mTc-duramycin labelled medium EVs and small EVs intravenously to mice, we detected their accumulation primarily in the liver. In vitro, we found that medium EVs were primarily taken up by Kupffer cells, while small EV uptake was the highest in liver sinusoidal endothelial cells. Finally, we demonstrated that in hyperlipidemia, there was a decreased EV uptake both by Kupffer cells and liver sinusoidal endothelial cells. 
		Conclusions Our data suggest that hyperlipidema increases the release and reduces the uptake of EVs by liver cells. We also provide evidence for size-dependent differential EV uptake by the different cell types of the liver.
		Funding
		NVKP16-1-2016-0017, ÚNKP19-4-SE-09, János Bolyai Research Scholarship, Hungarian Scientific Research Fund (K120237), VEKOP2.3.2-16-2017-000002, VEKOP2.3.3-15-2017-00016, H2020-MSCA-ITN-2017-722148 TRAIN EV, FIKP-Therapeutic Thematic Programme, Horizon 2020 Research and Innovation Programme (739593)},
	year = {2021},
	orcid-numbers = {Németh, Krisztina/0000-0002-3825-2137; Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Péter/0000-0001-7374-667X; Tamási, Viola/0000-0001-7419-5603}
}