TY - JOUR AU - Gál, Eleonóra AU - Veréb, Zoltán AU - Rakk, Dávid AU - Szekeres, András AU - Becskeházi, Eszter AU - Tiszlavicz, László AU - Czakó, László AU - Takács, Tamás AU - Hegyi, P AU - Venglovecz, Viktória TI - The effect of bile on pancreatic cancer, the importance of mucins JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 8 PY - 2022 IS - Suppl. 1 SP - 77 EP - 77 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32843481 ID - 32843481 LA - English DB - MTMT ER - TY - JOUR AU - Korsós, Marietta Margaréta AU - Bellák, Tamás AU - Becskeházi, Eszter AU - Gál, Eleonóra AU - Veréb, Zoltán AU - Venglovecz, Viktória TI - Mouse esophaegal, organoid cultures are the novel model for studying the epithelial ion transport mechanisms JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 8 PY - 2022 IS - Suppl. 1 SP - 85 EP - 85 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32843357 ID - 32843357 LA - English DB - MTMT ER - TY - JOUR AU - Becskeházi, Eszter AU - Korsós, Marietta Margaréta AU - Gál, Eleonóra AU - Tiszlavicz, László AU - Hoyk, Z AU - Deli, M AU - Köhler, Zoltán Márton AU - Keller-Pintér, Anikó AU - Horváth, Attila AU - Csekő, K AU - Helyes, Z AU - Hegyi, Péter AU - Venglovecz, Viktória TI - NHE-1 plays a central role in the smoke-induced proliferation in barrett's esophageal cell line JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 8 PY - 2022 IS - Suppl 1 SP - 65 EP - 65 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32843331 ID - 32843331 LA - English DB - MTMT ER - TY - JOUR AU - Becskeházi, Eszter AU - Korsós, Marietta Margaréta AU - Gál, Eleonóra AU - Tiszlavicz, László AU - Hoyk, Zsófia AU - Deli, Mária Anna AU - Köhler, Zoltán Márton AU - Keller-Pintér, Anikó AU - Horváth, Attila AU - Csekő, Kata AU - Helyes, Zsuzsanna AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 19 PG - 16 SN - 1661-6596 DO - 10.3390/ijms221910581 UR - https://m2.mtmt.hu/api/publication/32258572 ID - 32258572 LA - English DB - MTMT ER - TY - JOUR AU - Korsós, Marietta Margaréta AU - Bellák, Tamás AU - Becskeházi, Eszter AU - Gál, Eleonóra AU - Veréb, Zoltán AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Mouse organoid culture is a suitable model to study esophageal ion transport mechanisms JF - AMERICAN JOURNAL OF PHYSIOLOGY: CELL PHYSIOLOGY J2 - AM J PHYSIOL CELL PH VL - 321 PY - 2021 IS - 5 SP - C798 EP - C811 PG - 14 SN - 0363-6143 DO - 10.1152/ajpcell.00295.2021 UR - https://m2.mtmt.hu/api/publication/32204160 ID - 32204160 N1 - Export Date: 26 January 2024 CODEN: AJPCD Correspondence Address: Venglovecz, V.; Department of Pharmacology and Pharmacotherapy, Hungary; email: venglovecz.viktoria@med.u-szeged.hu AB - Altered esophageal ion transport mechanisms play a key role in inflammatory and cancerous diseases of the esophagus, but epithelial ion processes have been less studied in the esophagus because of the lack of a suitable experimental model. In this study, we generated 3D esophageal organoids (EOs) from two different mouse strains and characterized the ion transport processes of the EOs. EOs form a cell-filled structure with a diameter of 250-300 µm and generated from epithelial stem cells as shown by FACS analysis. Using conventional PCR and immunostaining, the presence of Slc26a6 Cl-/HCO3- anion exchanger (AE), Na+/H+ exchanger (NHE), Na+/HCO3- cotransporter (NBC), cystic fibrosis transmembrane conductance regulator (CFTR) and anoctamin 1 Cl- channels were detected in EOs. Microfluorimetric techniques revealed high NHE, AE, and NBC activities, whereas that of CFTR was relatively low. In addition, inhibition of CFTR led to functional interactions between the major acid-base transporters and CFTR. We conclude that EOs provide a relevant and suitable model system for studying the ion transport mechanisms of esophageal epithelial cells, and they can be also used as preclinical tools to assess the effectiveness of novel therapeutic compounds in esophageal diseases associated with altered ion transport processes. LA - English DB - MTMT ER - TY - JOUR AU - Gál, Eleonóra AU - Veréb, Z. AU - Rakk, Dávid AU - Szekeres, András AU - Becskeházi, Eszter AU - Kemény, L. AU - Tiszlavicz, L. AU - Czakó, László AU - Takács, Tamás AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Effects of bile acids on pancreatic ductal adenocarcinoma cells JF - PANCREATOLOGY J2 - PANCREATOLOGY VL - 21 PY - 2021 SP - S73 EP - S74 PG - 2 SN - 1424-3903 DO - 10.1016/j.pan.2021.05.197 UR - https://m2.mtmt.hu/api/publication/32167463 ID - 32167463 LA - English DB - MTMT ER - TY - JOUR AU - Korsós, Marietta Margaréta AU - Bellák, Tamás AU - Becskeházi, Eszter AU - Venglovecz, Viktória TI - Esophageal organoid culture is a novel model to study epithelial ion transport mechanisms JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 6 PY - 2020 IS - Suppl 2 SP - 62 EP - 62 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32104411 ID - 32104411 LA - English DB - MTMT ER - TY - JOUR AU - Becskeházi, Eszter AU - Gál, Eleonóra AU - Korsós, Marietta Margaréta AU - Venglovecz, Viktória TI - Smoking changes iontransport mechanisms of guinea pig esophageal epithelial cells and human esophageal cell lines JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 6 PY - 2020 IS - Suppl 2 SP - 35 EP - 35 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/32104401 ID - 32104401 LA - English DB - MTMT ER - TY - JOUR AU - Gál, Eleonóra AU - Veréb, Zoltán AU - Rakk, Dávid AU - Szekeres, András AU - Becskeházi, Eszter AU - Kemény, Lajos AU - Tiszlavicz, László AU - Czakó, László AU - Takács, Tamás AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Importance of bile acids in the progression of pancreatic cancer JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 6 PY - 2020 IS - Suppl 2 SP - 51 EP - 51 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/31784517 ID - 31784517 LA - English DB - MTMT ER - TY - JOUR AU - Gál, Eleonóra AU - Veréb, Zoltán AU - Kemény, Lajos AU - Rakk, Dávid AU - Szekeres, András AU - Becskeházi, Eszter AU - Tiszlavicz, László AU - Takács, Tamás AU - Czakó, László AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Bile accelerates carcinogenic processes in pancreatic ductal adenocarcinoma cells through the overexpression of MUC4 JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 10 PY - 2020 IS - 1 PG - 16 SN - 2045-2322 DO - 10.1038/s41598-020-79181-6 UR - https://m2.mtmt.hu/api/publication/31784466 ID - 31784466 N1 - Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, 6720, Hungary Regenerative Medicine and Cellular Pharmacology Research Laboratory, Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary HCEMM SZTE Skin Research Group, University of Szeged, Szeged, Hungary Department of Microbiology, University of Szeged, Szeged, Hungary Department of Pathology, University of Szeged, Szeged, Hungary First Department of Medicine, University of Szeged, Szeged, Hungary Institute for Translational Medicine, Medical School, Szentágothai Research Centre, University of Pécs, Pécs, Hungary Division of Gastroenterology, First Department of Medicine, Medical School, University of Pécs, Pécs, Hungary Cited By :9 Export Date: 26 January 2024 Correspondence Address: Venglovecz, V.; Department of Pharmacology and Pharmacotherapy, Hungary; email: venglovecz.viktoria@med.u-szeged.hu AB - Pancreatic cancer (PC) is one of the leading causes of mortality rate globally and is usually associated with obstructive jaundice (OJ). Up to date, there is no clear consensus on whether biliary decompression should be performed prior to surgery and how high levels of serum bile affects the outcome of PC. Therefore, our study aims were to characterise the effect of bile acids (BAs) on carcinogenic processes using pancreatic ductal adenocarcinoma (PDAC) cell lines and to investigate the underlying mechanisms. Liquid chromatography-mass spectrometry was used to determine the serum concentrations of BAs. The effects of BAs on tumour progression were investigated using different assays. Mucin expressions were studied in normal and PDAC cell lines and in human samples at gene and protein levels and results were validated with gene silencing. The levels of BAs were significantly higher in the PDAC + OJ group compared to the healthy control. Treating PDAC cells with different BAs or with human serum obtained from PDAC + OJ patients enhanced the rate of proliferation, migration, adhesion, colony forming, and the expression of MUC4. In PDAC + OJ patients, MUC4 expression was higher and the 4-year survival rate was lower compare to PDAC patients. Silencing of MUC4 decreased BAs-induced carcinogenic processes in PDAC cells. Our results show that BAs promote carcinogenic process in PDAC cells, in which the increased expression of MUC4 plays an important role. Based on these results, we assume that in PC patients, where the disease is associated with OJ, the early treatment of biliary obstruction improves life expectancy. LA - English DB - MTMT ER -