@article{MTMT:36077689,
	title = {Heated Aquatic Shelters Reduce Infection Intensity but Not Prevalence of a Fungal Pathogen in Common Toad Tadpoles},
	url = {https://m2.mtmt.hu/api/publication/36077689},
	author = {Kásler, Andrea and Ujszegi, János and Herczeg, Dávid and Holly, Dóra and Mikó, Zsanett and Hettyey, Attila},
	doi = {10.1111/acv.70012},
	journal-iso = {ANIM CONSERV},
	journal = {ANIMAL CONSERVATION},
	unique-id = {36077689},
	issn = {1367-9430},
	abstract = {The development of in situ applicable mitigation methods against diseases is an area in conservation biology that deserves more attention. The chytrid fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, an infectious disease in amphibians. Although it is responsible for population declines and extinctions worldwide, no widely applicable mitigation method is available against the disease. The fungus has a lower thermal tolerance than most amphibian species, which raises the possibility of curing individuals with thermal treatments, an alternative that is likely safer to use under natural conditions than chemicals. Amphibians, being ectothermic animals, actively seek warm environments, so presenting them with the possibility of reaching their preferred body temperatures may be a mild but effective mitigation intervention. In this study, we tested whether providing a heated shelter for Bd‐exposed common toad ( Bufo bufo ) tadpoles, kept under seminatural conditions at different densities, helps the individuals to eliminate the infection or reduce its costs. We found that providing tadpoles with access to heated shelters decreased the intensity but not the prevalence of Bd‐infection, and that the density of tadpoles did not alter the beneficial effect of the treatment. The mean body mass of individuals was lowered when tadpoles were kept at high density, but the availability of heated shelters did not have an effect on the body mass of individuals. Our results suggest that the application of heated microrefugia may help Bd‐infected common toad tadpoles overcome the disease, but the device itself and its application both need further improvements. The use of heated shelters could become a cheap and widely applicable method for the in situ treatment of chytridiomycosis and other waterborne, cold‐adapted pathogens in nature conservation.},
	year = {2025},
	eissn = {1469-1795},
	orcid-numbers = {Kásler, Andrea/0000-0003-2075-0776; Herczeg, Dávid/0000-0001-5098-961X; Hettyey, Attila/0000-0003-0678-0936}
}

@article{MTMT:36407997,
	title = {Effects of pyrethroid insecticides on sex ratios in agile frogs (Rana dalmatina)},
	url = {https://m2.mtmt.hu/api/publication/36407997},
	author = {Balogh, Emese and Hócza, Szabolcs and Ujhegyi, Nikolett and Kásler, Andrea and Holly, Dóra and Herczeg, Dávid and Ujszegi, János and Gál, Zoltán and Hoffmann, Orsolya Ivett and Bókony, Veronika and Mikó, Zsanett},
	doi = {10.1016/j.aquatox.2025.107624},
	journal-iso = {AQUAT TOXICOL},
	journal = {AQUATIC TOXICOLOGY},
	volume = {290},
	unique-id = {36407997},
	issn = {0166-445X},
	abstract = {Environmental pollutants have the potential to alter sex ratios in wildlife through sex-biased mortality. Furthermore, endocrine disruptors may cause sex reversal during early ontogeny in ectothermic vertebrates, resulting in a phenotypic sex that is not concordant with the genotypic sex encoded by the sex chromosomes. Despite the wide-ranging implications of these sex-ratio biasing effects, they are rarely studied in ecotoxicology, especially in a way that allows for disentangling the two mechanisms. We investigated these effects of two synthetic pyrethroids, deltamethrin and etofenprox, that are commonly used insecticides and have been linked to adverse effects on fish and amphibian biodiversity. We assessed the effects of environmentally relevant concentrations of these two pyrethroids on phenotypic sex ratio, sex-dependent mortality, and sex reversal in agile frogs (Rana dalmatina). Tadpoles from field-collected eggs were reared in mesocosm until metamorphosis by adding 0.03 or 0.3 μg/L of deltamethrin or etofenprox three times to the water. We observed no effect in three of the four treatment groups. However, in the lower-concentration etofenprox treatment, phenotypic sex ratio was male-biased two months post-metamorphosis, and genotypic sexing revealed that this was due to female-biased mortality during metamorphosis and not to sex reversal. Although the estimation certainty of these effects was somewhat limited, they highlight that not all sex-ratio distorting effects are caused by sex reversal. Therefore, ecotoxicological studies aiming to understand the endocrine distruptor effects of environmental contaminants should strive to separate the effects on sex determination and sex-dependent mortality.},
	year = {2025},
	eissn = {1879-1514},
	orcid-numbers = {Ujhegyi, Nikolett/0000-0001-9610-0353; Herczeg, Dávid/0000-0001-5098-961X; Bókony, Veronika/0000-0002-2136-5346}
}

@article{MTMT:35454226,
	title = {Juvenile agile frogs spatially avoid ranavirus-infected conspecifics},
	url = {https://m2.mtmt.hu/api/publication/35454226},
	author = {Herczeg, Dávid and Horváth, Gergely and Bókony, Veronika and Herczeg, Gábor and Kásler, Andrea and Holly, Dóra and Mikó, Zsanett and Ujhegyi, Nikolett and Ujszegi, János and Papp, Tibor and Hettyey, Attila},
	doi = {10.1038/s41598-024-74575-2},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {35454226},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X; Horváth, Gergely/0000-0002-0485-333X; Bókony, Veronika/0000-0002-2136-5346; Herczeg, Gábor/0000-0003-0441-342X; Ujhegyi, Nikolett/0000-0001-9610-0353; Papp, Tibor/0000-0002-6402-3864}
}

@article{MTMT:33582820,
	title = {Chytridiomycosis and climate change: exposure to Batrachochytrium dendrobatidis and mild winter conditions do not increase mortality in juvenile agile frogs during hibernation},
	url = {https://m2.mtmt.hu/api/publication/33582820},
	author = {Kásler, Andrea and Holly, Dóra and Herczeg, Dávid and Ujszegi, János and Hettyey, Attila},
	doi = {10.1111/acv.12851},
	journal-iso = {ANIM CONSERV},
	journal = {ANIMAL CONSERVATION},
	volume = {26},
	unique-id = {33582820},
	issn = {1367-9430},
	year = {2023},
	eissn = {1469-1795},
	pages = {654-662},
	orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X}
}

@CONFERENCE{MTMT:33661330,
	title = {Ökotoxikológia egy komplex világban: peszticidek és patogének együttes hatásai erdei békákra (Rana dalmatina) a korai életszakaszban},
	url = {https://m2.mtmt.hu/api/publication/33661330},
	author = {Hettyey, Attila and Kásler, Andrea and Ujszegi, János and Holly, Dóra and Herczeg, Dávid},
	booktitle = {69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK},
	unique-id = {33661330},
	year = {2023},
	pages = {34},
	orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X}
}

@CONFERENCE{MTMT:33661348,
	title = {A klímaváltozás és két piretroid rovarölőszer együttes hatásai juvenilis barna varangyok (Bufo bufo) fejlődésére és túlélésére},
	url = {https://m2.mtmt.hu/api/publication/33661348},
	author = {Kásler, Andrea and Bókony, Veronika and Mikó, Zsanett and Herczeg, Dávid and Ujszegi, János and Holly, Dóra and Hettyey, Attila},
	booktitle = {69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK},
	unique-id = {33661348},
	year = {2023},
	pages = {36},
	orcid-numbers = {Bókony, Veronika/0000-0002-2136-5346; Herczeg, Dávid/0000-0001-5098-961X}
}

@article{MTMT:34010493,
	title = {Does The Glucocorticoid Stress Response Make Toads More Toxic? An Experimental Study on The Regulation of Bufadienolide Toxin Synthesis},
	url = {https://m2.mtmt.hu/api/publication/34010493},
	author = {Üveges, Bálint and Kalina, C and Szabó, Krisztina and Móricz, Ágnes and Holly, Dóra and Gabor, C R and Hettyey, Attila and Bókony, Veronika},
	doi = {10.1093/iob/obad021},
	journal-iso = {INTEGR ORGAN BIOL},
	journal = {INTEGRATIVE ORGANISMAL BIOLOGY},
	volume = {5},
	unique-id = {34010493},
	abstract = {Chemical defence is a crucial component of fitness in many organisms, yet the physiological regulation of defensive toxin synthesis is poorly understood, especially in vertebrates. Bufadienolides, the main defensive compounds of toads, are toxic to many predators and other natural enemies, and their synthesis can be upregulated by stressors including predation risk, high conspecific density, and pollutants. Thus, higher toxin content may be the consequence of a general endocrine stress response in toads. Therefore, we hypothesized that bufadienolide synthesis may be stimulated by elevated levels of corticosterone (CORT), the main glucocorticoid hormone of amphibians, or by upstream regulators that stimulate CORT production. To test these alternatives, we treated common toad tadpoles with exogenous CORT (exoCORT) or metyrapone (MTP, a CORT-synthesis inhibitor that stimulates upstream regulators of CORT by negative feedback) in the presence or absence of predation cues for 2 or 6 days, and subsequently measured their CORT release rates and bufadienolide content. We found that CORT release rates were elevated by exoCORT, and to a lesser extent also by MTP, regardless of treatment length. Bufadienolide content was significantly decreased by treatment with exoCORT for 6 days, but was unaffected by exposure to exoCORT for 2 days or to MTP for either 6 or 2 days. Presence or absence of predation cues affected neither CORT release rate nor bufadienolide content. Our results suggest that changes in bufadienolide synthesis in response to environmental challenges are not driven by CORT but may rather be regulated by upstream hormones of the stress response.},
	year = {2023},
	eissn = {2517-4843},
	orcid-numbers = {Üveges, Bálint/0000-0001-9234-9258; Gabor, C R/0000-0001-7584-1451; Bókony, Veronika/0000-0002-2136-5346}
}

@article{MTMT:34074949,
	title = {Amphibian larvae benefit from a warm environment under simultaneous threat from chytridiomycosis and ranavirosis},
	url = {https://m2.mtmt.hu/api/publication/34074949},
	author = {Herczeg, Dávid and Holly, Dóra and Kásler, Andrea and Bókony, Veronika and Papp, Tibor and Takács-Vágó, Hunor and Ujszegi, János and Hettyey, Attila},
	doi = {10.1111/oik.09953},
	journal-iso = {OIKOS},
	journal = {OIKOS},
	volume = {2023},
	unique-id = {34074949},
	issn = {0030-1299},
	abstract = {Rising temperatures can facilitate epizootic outbreaks, but disease outbreaks may be suppressed if temperatures increase beyond the optimum of the pathogens while still within the temperature range that allows for effective immune function in hosts. The two most devastating pathogens of wild amphibians, <jats:italic>Batrachochytrium dendrobatidis</jats:italic> (<jats:italic>Bd</jats:italic>) and ranaviruses (<jats:italic>Rv</jats:italic>), co‐occur in large areas, yet little is known about the consequences of their co‐infection and how these consequences depend on temperature. Here we tested how exposure to <jats:italic>Bd</jats:italic> and subsequent exposure to <jats:italic>Rv</jats:italic>, followed by treatment at elevated temperatures (28 and 30°C versus 22°C) affected <jats:italic>Bd</jats:italic> and <jats:italic>Rv</jats:italic> prevalence, infection intensities, and resulting mortalities in larval agile frogs and common toads. We found multiple pieces of evidence that the presence of one pathogen influenced the prevalence and/or infection intensity of the other pathogen in both species, depending on temperature and initial <jats:italic>Rv</jats:italic> concentration. Generally, the 30°C treatment lowered the prevalence and infection intensity of both pathogens and, in agile frogs, this was mirrored by higher survival. These results suggest that if temperatures naturally increase or are artificially elevated beyond what is ideal for both <jats:italic>Bd</jats:italic> and <jats:italic>Rv</jats:italic>, amphibians may be able to control infections and survive even the simultaneous presence of their most dangerous pathogenic enemies.},
	year = {2023},
	eissn = {1600-0706},
	orcid-numbers = {Herczeg, Dávid/0000-0001-5098-961X; Bókony, Veronika/0000-0002-2136-5346; Papp, Tibor/0000-0002-6402-3864}
}

@article{MTMT:32837173,
	title = {Metamorphic common toads keep chytrid infection under control, but at a cost},
	url = {https://m2.mtmt.hu/api/publication/32837173},
	author = {Kásler, Andrea and Ujszegi, János and Holly, Dóra and Üveges, Bálint and Móricz, Ágnes and Herczeg, Dávid and Hettyey, Attila},
	doi = {10.1111/jzo.12974},
	journal-iso = {J ZOOL},
	journal = {JOURNAL OF ZOOLOGY},
	volume = {317},
	unique-id = {32837173},
	issn = {0952-8369},
	year = {2022},
	eissn = {1469-7998},
	pages = {159-169},
	orcid-numbers = {Üveges, Bálint/0000-0001-9234-9258; Herczeg, Dávid/0000-0001-5098-961X}
}

@article{MTMT:32888499,
	title = {In vitro thermal tolerance of a hypervirulent lineage of Batrachochytrium dendrobatidis : Growth arrestment by elevated temperature and recovery following thermal treatment},
	url = {https://m2.mtmt.hu/api/publication/32888499},
	author = {Kásler, Andrea and Ujszegi, János and Holly, Dóra and Jaloveczki, Boglárka and Gál, Zoltán and Hettyey, Attila},
	doi = {10.1080/00275514.2022.2065443},
	journal-iso = {MYCOLOGIA},
	journal = {MYCOLOGIA},
	volume = {114},
	unique-id = {32888499},
	issn = {0027-5514},
	year = {2022},
	eissn = {1557-2536},
	pages = {661-669}
}