TY - THES AU - Pál, Sára Eszter TI - Overexpressziós mutáns törzsek létrehozása és széles körű vizsgálata az opportunista humánpatogén Candida parapsilosis gombafajban [Establishment and extensive investigation of an overexpression mutant collection in opportunist human pathogenic Candida parapsilosis] PB - Szegedi Tudományegyetem PY - 2022 SP - 140 DO - 10.14232/phd.11334 UR - https://m2.mtmt.hu/api/publication/34110227 ID - 34110227 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pál, Sára Eszter AU - Tóth, Renáta AU - Nosanchuk, Joshua D. AU - Vágvölgyi, Csaba AU - Németh, Tibor Mihály AU - Gácser, Attila TI - A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 7 PY - 2021 IS - 2 PG - 22 SN - 2309-608X DO - 10.3390/jof7020097 UR - https://m2.mtmt.hu/api/publication/31844041 ID - 31844041 N1 - Funding Agency and Grant Number: Richter Gedeon Talentum Foundation; Hungarian Academy of SciencesHungarian Academy of Sciences; [20391-3/2018/FEKUSTRAT]; [NKFIH K 123952]; [GINOP-2.3.2.-15-2016-00035]; [LP2018-15/2018] Funding text: This work was supported by grants 20391-3/2018/FEKUSTRAT, NKFIH K 123952, and GINOP-2.3.2.-15-2016-00035. S.E.P. was funded by Richter Gedeon Talentum Foundation. A.G. was further funded by LP2018-15/2018. T.N. was supported by the Postdoctoral Fellowship Programme of the Hungarian Academy of Sciences. Department of Microbiology, University of Szeged, Közép Fasor, Szeged, 6726, Hungary Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, United States MTA-SZTE Lendület Mycobiome Research Group, University of Szeged, Szeged, 6726, Hungary Export Date: 2 August 2021 Correspondence Address: Gácser, A.; Department of Microbiology, Közép Fasor, Hungary; email: gacsera@bio.u-szeged.hu Correspondence Address: Gácser, A.; MTA-SZTE Lendület Mycobiome Research Group, Hungary; email: gacsera@bio.u-szeged.hu Department of Microbiology, University of Szeged, Közép Fasor, Szeged, 6726, Hungary Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, United States MTA-SZTE Lendület Mycobiome Research Group, University of Szeged, Szeged, 6726, Hungary Export Date: 16 August 2021 Correspondence Address: Gácser, A.; Department of Microbiology, Közép Fasor, Hungary; email: gacsera@bio.u-szeged.hu Correspondence Address: Gácser, A.; MTA-SZTE Lendület Mycobiome Research Group, Hungary; email: gacsera@bio.u-szeged.hu Department of Microbiology, University of Szeged, Közép Fasor, Szeged, 6726, Hungary Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, United States MTA-SZTE Lendület Mycobiome Research Group, University of Szeged, Szeged, 6726, Hungary Export Date: 17 August 2021 Correspondence Address: Gácser, A.; Department of Microbiology, Közép Fasor, Hungary; email: gacsera@bio.u-szeged.hu Correspondence Address: Gácser, A.; MTA-SZTE Lendület Mycobiome Research Group, Hungary; email: gacsera@bio.u-szeged.hu Department of Microbiology, University of Szeged, Közép Fasor, Szeged, 6726, Hungary Departments of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY 10461, United States MTA-SZTE Lendület Mycobiome Research Group, University of Szeged, Szeged, 6726, Hungary Export Date: 18 August 2021 Correspondence Address: Gácser, A.; Department of Microbiology, Közép Fasor, Hungary; email: gacsera@bio.u-szeged.hu Correspondence Address: Gácser, A.; MTA-SZTE Lendület Mycobiome Research Group, Hungary; email: gacsera@bio.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Gerőcs, Annamária AU - Nemes-Barnas, Katalin AU - Pál, Sára Eszter AU - Szoke, Barna AU - Majer, Janos AU - Farkas, Tibor AU - Olasz, Ferenc TI - Isolation and characterization of yeast strains from Badacsony, Hungary JF - INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY J2 - INDIAN J EXP BIOL VL - 58 PY - 2020 IS - 7 SP - 461 EP - 473 PG - 13 SN - 0019-5189 UR - https://m2.mtmt.hu/api/publication/31740716 ID - 31740716 AB - In modern winery, starter strains are used for wine making to avoid the risk of slow or incomplete fermentation. However, application of commercial starter yeasts sometimes leads to a uniform character of the wines. On the other hand, indigenous ("terroir") strains are adapted better to local conditions highlighting the specific taste of wine. In this study, we isolated local yeast strains from Badacsony wine region of Hungary and investigated with microbiological and molecular biological tests in order to develop indigenous starter selection method. As many as 480 yeast strains were isolated and grouped using carbohydrate and nitrogen sources. Finally, 80 selected isolates were characterized for important oenological features, including tolerance of glucose, ethanol and acetic acid. Fermentation ability, killer toxin, hydrogen sulfide and acid production of 80 selected isolates were also tested. Isolates were studied by applying two molecular methods based on rRNA gene sequencing and analysis of Ty retrotransposon's delta elements in case of Saccharomyces strains. Our results have shown that the isolated strains belong to 15 yeast species of 8 genera, and the diversity of yeast population was significantly high in the investigated vineyard. We have found that selection for technological properties was a potential way to find suitable strains from the local microbiome, because a high proportion of isolated wild yeast strains show beneficial oenological properties for wine making. Further, we studied 35 available starter yeasts to avoid re-isolation and we identified only 3 starter yeasts from grape and must samples, which can be considered as very low incidence. LA - English DB - MTMT ER - TY - JOUR AU - Gerőcs, Annamária AU - Pál, Sára Eszter AU - K., Nemes-Barnás AU - Májer, János AU - Szőke, Barna AU - Olasz, Ferenc TI - Simple methods for selection of Saccharomyces starter yeast from local microbiom JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 66 PY - 2019 IS - Suppl. 1. SP - - SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/31237086 ID - 31237086 LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Anda, Dóra AU - Kériné Borsodi, Andrea AU - Szabó, Attila AU - Pál, Sára Eszter AU - Óvári, Mihály AU - Márialigeti, Károly AU - Kovácsné Bodor, Petra AU - Mádlné Szőnyi, Judit AU - Makk, Judit TI - Radioactive environment adapted bacterial communities constituting the biofilms of hydrothermal spring caves (Budapest, Hungary) JF - JOURNAL OF ENVIRONMENTAL RADIOACTIVITY J2 - J ENVIRON RADIOACTIV VL - 203 PY - 2019 SP - 8 EP - 17 PG - 10 SN - 0265-931X DO - 10.1016/j.jenvrad.2019.02.010 UR - https://m2.mtmt.hu/api/publication/30539729 ID - 30539729 N1 - Cited By :3 Export Date: 29 October 2019 AB - The thermal waters of Gellért Hill discharge area of the Buda Thermal Karst System (Hungary) are characterized by high (up to 1000 Bq/L) 222Rn-activity due to the radium-accumulating biogeochemical layers. Samples were taken from these ferruginous and calcareous layers developed on spring cave walls and water surface. Accumulation of potentially toxic metals (e.g. As, Hg, Pb, Sn, Sr, Zn) in the dense extracellular polymeric substance containing bacterial cells and remains was detected by inductively coupled plasma mass spectrometry. The comparison of bacterial phylogenetic diversity of the biofilm samples was performed by high throughput next generation sequencing (NGS). The analysis showed similar sets of mainly unidentified taxa of phyla Chloroflexi, Nitrospirae, Proteobacteria, Planctomycetes; however, large differences were found in their abundance. Cultivation-based method complemented with irradiation assay was performed using 5, 10 and 15 kGy doses of gamma-rays from a 60Co-source to reveal the extreme radiation-resistant bacteria. The phyla Actinobacteria, Firmicutes, Proteobacteria (classes Alpha- Beta- and Gammaproteobacteria), Bacteriodetes and Deinococcus-Thermus were represented among the 452 bacterial strains. The applied irradiation treatments promoted the isolation of 100 different species, involving candidate novel species, as well. The vast majority of the isolates belonged to bacterial taxa previously unknown as radiation-resistant microorganisms. Members of the genera Paracoccus, Marmoricola, Dermacoccus and Kytococcus were identified from the 15 kGy dose irradiated samples. The close relatives of several known radiation-tolerant bacteria were also detected from the biofilm samples, alongside with bacteria capable of detoxification by metal accumulation, adsorption and precipitation in the form of calcium-carbonate which possibly maintain the viability of the habitat. The results suggest the establishment of a unique, extremophilic microbiota in the studied hydrothermal spring caves. LA - English DB - MTMT ER - TY - JOUR AU - Pál, Sára Eszter AU - Németh, Tibor Mihály AU - Pryszcz, L AU - Gabaldon, T AU - Vágvölgyi, Csaba AU - Gácser, Attila TI - Generation and characterization of Candida parapsilosis over-expression mutant strains JF - MEDICAL MYCOLOGY J2 - MED MYCOL VL - 56 PY - 2018 IS - S2 SP - S138 EP - S138 PG - 1 SN - 1369-3786 UR - https://m2.mtmt.hu/api/publication/3412109 ID - 3412109 LA - English DB - MTMT ER - TY - JOUR AU - Gerőcs, Annamária AU - Pál, Sára Eszter AU - K., Nemes-Barnás AU - Májer, János AU - Szőke, Barna AU - Olasz, Ferenc TI - Biological diversity of yeast species from must samples of the Badacsony wine region JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 64 PY - 2017 IS - Suppl 1 SP - 128 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/31237025 ID - 31237025 LA - English DB - MTMT ER - TY - JOUR AU - Németh, Tibor Mihály AU - Pál, Sára Eszter AU - Pryszcz, Leszek AU - Vágvölgyi, Csaba AU - Gabaldon, Toni AU - Gácser, Attila TI - Molekuláris technikák kidolgozása a humán patogén Candida parapsilosis virulenciájának tanulmányozására = Developing molecular techniques for studying the virulence of the human fungal pathogen Candida Parapsilosis JF - MIKOLÓGIAI KÖZLEMÉNYEK-CLUSIANA J2 - MIKOLÓGIAI KÖZLEMÉNYEK-CLUSIANA PY - 2017 SP - 59 EP - 60 PG - 2 SN - 0133-9095 UR - https://m2.mtmt.hu/api/publication/3410359 ID - 3410359 LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Anda, Dóra AU - Kériné Borsodi, Andrea AU - Pál, Sára Eszter AU - Óvári, Mihály AU - Márialigeti, Károly AU - Kovácsné Bodor, Petra AU - Mádlné Szőnyi, Judit AU - Makk, Judit TI - Cultivation-based identification of bacterial communities inhabiting the radioactive hydrothermal spring caves of Gellért Hill, Hungary JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 64 PY - 2017 IS - Suppl 1 SP - 121 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/3301604 ID - 3301604 LA - English DB - MTMT ER - TY - CONF AU - Németh, Tibor Mihály AU - Pál, Sára Eszter AU - Leszek, Pryszcz AU - Toni, Gabaldon AU - Gácser, Attila TI - Molecular tools for studying Candida parapsilosis virulence T2 - Human Fungal Pathogens C1 - La Colle sur Loup PY - 2017 SP - 141 UR - https://m2.mtmt.hu/api/publication/3233655 ID - 3233655 LA - English DB - MTMT ER -