TY - JOUR AU - Csomos, Attila AU - Madarász, Miklós AU - Turczel, Gábor AU - Cseri, Levente AU - Katona, Gergely AU - Rózsa J., Balázs AU - Kovács, Ervin AU - Mucsi, Zoltán TI - A Molecular Hybrid of the GFP Chromophore and 2,2′-Bipyridine: An Accessible Sensor for Zn2+ Detection with Fluorescence Microscopy JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 6 PG - 14 SN - 1661-6596 DO - 10.3390/ijms25063504 UR - https://m2.mtmt.hu/api/publication/34748236 ID - 34748236 N1 - Department of Chemistry, Femtonics Ltd, Tűzoltó utca 59, Budapest, H-1094, Hungary Hevesy György PhD School of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, H-1117, Hungary BrainVisionCenter, Liliom utca 43, Budapest, H-1094, Hungary NMR Research Laboratory, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok körútja 2, Budapest, H-1117, Hungary Department of Organic Chemistry & Technology, Budapest University of Technology & Economics, 3. Muegyetem rakpart, Budapest, H-1111, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Práter utca 50/a, Budapest, H-1083, Hungary Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Szigony utca 43, Budapest, H-1083, Hungary Polymer Chemistry and Physics Research Group, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok körútja 2, Budapest, H-1117, Hungary Faculty of Materials and Chemical Sciences, University of Miskolc, Miskolc, H-3515, Hungary Export Date: 12 April 2024 Correspondence Address: Rózsa, B.; BrainVisionCenter, Liliom utca 43, Hungary; email: rozsabal@koki.hu Correspondence Address: Kovács, E.; Two-Photon Measurement Technology Research Group, Práter utca 50/a, Hungary; email: kovacs.ervin@ttk.hu AB - The few commercially available chemosensors and published probes for in vitro Zn2+ detection in two-photon microscopy are compromised by their flawed spectroscopic properties, causing issues in selectivity or challenging multistep syntheses. Herein, we present the development of an effective small molecular GFP chromophore-based fluorescent chemosensor with a 2,2′-bipyridine chelator moiety (GFZnP BIPY) for Zn2+ detection that has straightforward synthesis and uncompromised properties. Detailed experimental characterizations of the free and the zinc-bound compounds within the physiologically relevant pH range are presented. Excellent photophysical characteristics are reported, including a 53-fold fluorescence enhancement with excitation and emission maxima at 422 nm and 492 nm, respectively. A high two-photon cross section of 3.0 GM at 840 nm as well as excellent metal ion selectivity are reported. In vitro experiments on HEK 293 cell culture were carried out using two-photon microscopy to demonstrate the applicability of the novel sensor for zinc bioimaging. LA - English DB - MTMT ER - TY - JOUR AU - Csomos, Attila AU - Madarász, Miklós AU - Turczel, Gábor AU - Cseri, Levente AU - Bodor, Andrea AU - Anett, Matuscsák AU - Katona, Gergely AU - Kovács, Ervin AU - Rózsa J., Balázs AU - Mucsi, Zoltán TI - A GFP inspired 8-methoxyquinoline-derived fluorescent molecular sensor for the detection of Zn2+ by two-photon microscopy JF - CHEMISTRY-A EUROPEAN JOURNAL J2 - CHEM-EUR J PY - 2024 SN - 0947-6539 DO - 10.1002/chem.202400009 UR - https://m2.mtmt.hu/api/publication/34724516 ID - 34724516 AB - An effective, GFP-inspired fluorescent Zn2+ sensor is developed for two-photon microscopy and related biological application that featured an 8-methoxyquinoline moiety. Excellent photophysical characteristics including a 37-fold fluorescence enhancement with excitation and emission maxima at 440 nm and 505 nm, respectively, as well as a high two-photon cross-section of 73 GM at 880 nm are reported. Based on the experimental data, the relationship between the structure and properties was elucidated and explained backed up by DFT calculations, particularly to the observed PeT phenomenon for the turn-on process. Biological validation and detailed experimental and theoretical characterization of the free and the zinc-bound compounds are presented. LA - English DB - MTMT ER - TY - PAT AU - Csomos, Attila AU - Kovács, Ervin AU - Barkai, Gyöngy AU - Váradi, Csaba AU - Mucsi, Zoltán AU - Viskolcz, Béla AU - Rózsa J., Balázs AU - Cseri, Levente AU - Dojcsák, Dalma TI - Glikomikai alkalmazásra fejlesztett permanens töltéssel rendelkező fluoreszcens jelölővegyületek CY - Country:10001(1) PY - 2024 UR - https://m2.mtmt.hu/api/publication/34561430 ID - 34561430 AB - A bejelentés új, glikoproteinek elemzésére alkalmazható új jelölővegyületekre és kimutatási eljárásokra vonatkozik. Az új vegyületek N-hidroxi-szukcinimidil-karbamáttal származékolt xanténvázas rodamin- vagy pironin-származékok, amelyek N-glikánok folyadék-kromatográfiás elválasztást követő fluoreszcens és tömegspektrometriás detektálására használhatók fel. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Csomos, Attila AU - Kovács, Ervin AU - Madarász, Miklós AU - Fedor, Flóra Zsófia AU - Fülöp, Anna AU - Katona, Gergely AU - Rózsa J., Balázs AU - Mucsi, Zoltán TI - Two-Photon Fluorescent Chemosensors Based on the GFP-Chromophore for the Detection of Zn2+ in Biological Samples – From Design to Application JF - SENSORS AND ACTUATORS B-CHEMICAL J2 - SENSOR ACTUAT B CHEM VL - 398 PY - 2024 PG - 14 SN - 0925-4005 DO - 10.1016/j.snb.2023.134753 UR - https://m2.mtmt.hu/api/publication/34191513 ID - 34191513 LA - English DB - MTMT ER - TY - JOUR AU - Kontra, Bence AU - Bogdán, Dóra AU - Alaghehmand, Behta AU - Csomos, Attila AU - Dunkel, Petra TI - Synthesis and characterization of 8-aminoquinoline photocages for biological applications JF - TETRAHEDRON LETTERS J2 - TETRAHEDRON LETT VL - 124 PY - 2023 PG - 5 SN - 0040-4039 DO - 10.1016/j.tetlet.2023.154587 UR - https://m2.mtmt.hu/api/publication/33961930 ID - 33961930 N1 - Funding Agency and Grant Number: National Research, Develop- ment and Innovation Office (NKFIH) [SNN 135825, JNKP-21-5, JNKP-22-3]; National Research, Development and Innovation Office (NKFIH) [SNN 135825]; New National Excellence Program [JNKP-21-5, JNKP-22-3]; source of the National Research, Development and Innovation Fund [KDP-2021]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences Funding text: This research was funded by the National Research, Development and Innovation Office (NKFIH) grant number SNN 135825 and supported by the [JNKP-21-5 (P.D.) , [JNKP-22-3 (B.K.) New National Excellence Program and by the KDP-2021 Program of the Ministry of Innovation and Technology from the source of the National Research, Development and Innovation Fund. P.D. was recipient of the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. The contribution of our students (Dora Csor- ba, Martina Cavaliere) to the synthetic work is gratefully acknowledged. The authors thank Dr. Tamas Gati for the measurement and assignment of NMR spectra. LA - English DB - MTMT ER - TY - GEN AU - Máthé, Domokos AU - Szalay, Gergely AU - Cseri, Levente AU - Kis, Zoltán AU - Pályi, Bernadett AU - Földes, Gábor AU - Stelczerné Kovács, Noémi AU - Fülöp, Anna AU - Szepesi, Áron AU - Hajdrik, Polett AU - Csomos, Attila AU - Zsembery, Ákos AU - Kadar, Kristóf AU - Katona, Gergely AU - Mucsi, Zoltán AU - Rózsa J., Balázs AU - Kovács, Ervin TI - Monitoring correlates of SARS-CoV-2 infection in cell culture using two-photon microscopy and a novel fluorescent calcium-sensitive dye PY - 2023 PG - 61 UR - https://m2.mtmt.hu/api/publication/33252930 ID - 33252930 LA - English DB - MTMT ER - TY - PAT AU - Rózsa J., Balázs AU - Katona, Gergely AU - Mucsi, Zoltán AU - Kovács, Ervin AU - Csomos, Attila TI - Új, zöld fluoreszcens szenzorvegyületek fémionok kimutatására CY - Country:10001(1) PY - 2022 UR - https://m2.mtmt.hu/api/publication/33690395 ID - 33690395 LA - Hungarian DB - MTMT ER - TY - PAT AU - Rózsa J., Balázs AU - Katona, Gergely AU - Mucsi, Zoltán AU - Kovács, Ervin AU - Csomos, Attila TI - Új, piros fluoreszcens szenzorvegyületek fémionok kimutatására CY - Country:10001(1) PY - 2022 UR - https://m2.mtmt.hu/api/publication/33690384 ID - 33690384 LA - Hungarian DB - MTMT ER - TY - PAT AU - Rózsa J., Balázs AU - Katona, Gergely AU - Mucsi, Zoltán AU - Kovács, Ervin AU - Csomos, Attila TI - Red fluorescent sensor compounds for the detection of metal ions CY - Country:10001(1) PY - 2022 UR - https://m2.mtmt.hu/api/publication/33690369 ID - 33690369 LA - English DB - MTMT ER - TY - JOUR AU - Bogdán, Dóra AU - Kontra, Bence AU - Csomos, Attila AU - Kovács, Ervin AU - Mucsi, Zoltán AU - Dunkel, Petra TI - 3-Aminopropylazetidines: facile synthesis and application for medicinal chemical purposes JF - ARKIVOC J2 - ARKIVOC VL - 2022 PY - 2022 IS - 3 SP - 272 EP - 287 PG - 17 SN - 1551-7012 DO - 10.24820/ark.5550190.p011.900 UR - https://m2.mtmt.hu/api/publication/33289419 ID - 33289419 LA - English DB - MTMT ER -