TY - THES AU - Jánosi-Mózes, Emese TI - Innovative approach for the application of MTT, LDH and bis-ANS: ex vivo modelling of the extracellular beta-amyloid precipitation [Az MTT, az LDH és a bis-ANS újszerű alkalmazása: az extracelluláris béta-amiloid precipitációjának ex vivo modellezése] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 59 DO - 10.14232/phd.10783 UR - https://m2.mtmt.hu/api/publication/32475805 ID - 32475805 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Jánosi-Mózes, Emese AU - Szegedi, Viktor AU - Kálmán, János AU - Hunya, Ákos AU - Fülöp, Lívia AU - Tamano, Haruna AU - Takeda, Atsushi AU - Adlard, Paul A. AU - Bush, Ashley I. TI - Alzheimer risk factors age and female sex induce cortical Aβ aggregation by raising extracellular zinc [Alzheimer risk factors age and female sex induce cortical A beta aggregation by raising extracellular zinc] JF - MOLECULAR PSYCHIATRY J2 - MOL PSYCHIATR VL - 25 PY - 2020 IS - 11 SP - 2728 EP - 2741 PG - 14 SN - 1359-4184 DO - 10.1038/s41380-020-0800-y UR - https://m2.mtmt.hu/api/publication/31341681 ID - 31341681 AB - Aging and female sex are the major risk factors for Alzheimer's disease and its associated brain amyloid-beta (A beta) neuropathology, but the mechanisms mediating these risk factors remain uncertain. Evidence indicates that A beta aggregation by Zn(2+)released from glutamatergic neurons contributes to amyloid neuropathology, so we tested whether aging and sex adversely influences this neurophysiology. Using acute hippocampal slices, we found that extracellular Zn2+-elevation induced by high K(+)stimulation was significantly greater with older (65 weeks vs 10 weeks old) rats, and was exaggerated in females. This was driven by slower reuptake of extracellular Zn2+, which could be recapitulated by mitochondrial intoxication. Zn2+:A beta aggregates were toxic to the slices, but A beta alone was not. Accordingly, high K(+)caused synthetic human A beta added to the slices to form soluble oligomers as detected by bis-ANS, attaching to neurons and inducing toxicity, with older slices being more vulnerable. Age-dependent energy failure impairing Zn(2+)reuptake, and a higher maximal capacity for Zn(2+)release by females, could contribute to age and sex being major risk factors for Alzheimer's disease. LA - English DB - MTMT ER - TY - JOUR AU - Veszelka, Szilvia AU - Tóth, Andrea AU - Walter, Fruzsina AU - Datki, Zsolt László AU - Jánosi-Mózes, Emese AU - Fülöp, Lívia AU - Bozsó, Zsolt AU - Virághné Hellinger, Éva AU - Vastag, M AU - Orsolits, Barbara AU - Környei, Zsuzsanna AU - Penke, Botond AU - Deli, Mária Anna TI - Docosahexaenoic acid reduces amyloid β-induced toxicity in cells of the neurovascular unit JF - JOURNAL OF ALZHEIMER'S DISEASE J2 - J ALZHEIMERS DIS VL - 36 PY - 2013 IS - 3 SP - 487 EP - 501 PG - 15 SN - 1387-2877 DO - 10.3233/JAD-120163 UR - https://m2.mtmt.hu/api/publication/2246475 ID - 2246475 LA - English DB - MTMT ER - TY - JOUR AU - Földi, István AU - Tóth, Anikó M AU - Szabó, Zoltán AU - Jánosi-Mózes, Emese AU - Berkecz, Róbert AU - Datki, Zsolt László AU - Penke, Botond AU - Janáky, Tamás TI - Proteome-wide study of endoplasmic reticulum stress induced by thapsigargin in N2a neuroblastoma cells JF - NEUROCHEMISTRY INTERNATIONAL J2 - NEUROCHEM INT VL - 62 PY - 2013 IS - 1 SP - 58 EP - 69 PG - 12 SN - 0197-0186 DO - 10.1016/j.neuint.2012.11.003 UR - https://m2.mtmt.hu/api/publication/2148799 ID - 2148799 LA - English DB - MTMT ER - TY - JOUR AU - Jánosi-Mózes, Emese AU - Hunya, Ákos AU - Pósa, Anikó AU - Penke, Botond AU - Datki, Zsolt László TI - A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays JF - BRAIN RESEARCH BULLETIN J2 - BRAIN RES BULL VL - 87 PY - 2012 IS - 6 SP - 521 EP - 525 PG - 5 SN - 0361-9230 DO - 10.1016/j.brainresbull.2012.02.005 UR - https://m2.mtmt.hu/api/publication/2121113 ID - 2121113 AB - It is difficult task to measure precisely the toxic effect of beta-amyloid (Abeta 1-42) peptides and also the protective effect of novel drug candidates against Abeta-peptides. The widely used MTT-assay in cell lines or primary cell cultures could be insensitive against Abeta-peptides. We describe here an easy and relevant method for testing Abeta 1-42 toxicity on acute hippocampal slices derived from rat. Brain slice viability in different conditions was measured using MTT and LDH assays. The concomitant use of these two assays can give detailed and relevant results on the toxic effect of Abeta 1-42 in oxygen-glucose deprived (OGD) acute brain slice model. Both assays are capable of quantifying tissue viability by measuring optical density (OD). We found that simultaneous application of OGD and Abeta 1-42 treatment induced a more intensive decrease in hippocampal slice viability than their separate effects. The use of MTT and LDH assay for quantifying brain slice viability proved to be an easy ex vivo method for investigating Abeta toxicity. Testing brain slices is more relevant in Alzheimer's Disease research than using in vitro cell cultures, due to maintenance of the three dimensional cellular network, the cell variability and intact cell connections. LA - English DB - MTMT ER - TY - JOUR AU - Jánosi-Mózes, Emese AU - Hunya, Ákos AU - Toth, A AU - Ayaydin, Ferhan AU - Penke, Botond AU - Datki, Zsolt László TI - A novel application of the fluorescent dye bis-ANS for labeling neurons in acute brain slices JF - BRAIN RESEARCH BULLETIN J2 - BRAIN RES BULL VL - 86 PY - 2011 IS - 3-4 SP - 217 EP - 221 PG - 5 SN - 0361-9230 DO - 10.1016/j.brainresbull.2011.07.004 UR - https://m2.mtmt.hu/api/publication/1842704 ID - 1842704 AB - The cell-impermeant oligomer-(e.g. beta-amyloid-, or tubulin-) specific fluorescent dye, bis-ANS (4,4'-bis-1-anilinonaphtalene-8-sulfonate), was successfully used for labeling mechanically damaged but still viable neuron bodies, neurites and neurite cross sections in acute brain slices. Acute hippocampal brain slices of rats were co-stained with bis-ANS and the cell-impermeant. DNA-specific dye propidium iodide (PI) and were then analyzed using fluorescence and confocal microscopes. Both the neuron bodies and the neurites were found to exhibit increased fluorescence intensities, suggesting that using this method they can be detected more easily. In addition, bis-ANS showed good region - but not cell specific co-localization with the neuron-specific fluorescent dye Dil (1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate). These two dyes label different neuronal structures: Dil binds specifically to intact cell membranes while bis-ANS can enter cells with compromised cell membranes and then stain the microtubules in the cytoplasm. For a quick (10 min) staining of acute brain slices with bis-ANS both HEPES and NaHCO(3) were needed in order to achieve high signal intensity. Labeling with bis-ANS fluorescent dye is an easy method for imaging the neuronal structures on the surface of acute brain slices. (C) 2011 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER -