TY - JOUR AU - Shakeri, Abolfazl AU - Mirahmadi, Mohammad Reza AU - Kunert, Olaf AU - Tsai, Yu-Chi AU - Barta, Anita AU - Hohmann, Judit AU - Asili, Javad TI - Diverse diterpenoids and a triterpenoid from Euphorbia spinidens Bornm. ex Prokh JF - FITOTERAPIA J2 - FITOTERAPIA VL - 173 PY - 2024 PG - 5 SN - 0367-326X DO - 10.1016/j.fitote.2024.105838 UR - https://m2.mtmt.hu/api/publication/34556248 ID - 34556248 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Balázs AU - Szemerédi, Nikoletta AU - Kúsz, Norbert AU - Kiss, Tivadar AU - Csupor-Löffler, Boglárka AU - Tsai, Yu-Chi AU - Rácz, Bálint AU - Spengler, Gabriella AU - Csupor, Dezső TI - Antiproliferative and cytotoxic effects of sesquiterpene lactones isolated from Ambrosia artemisiifolia on human adenocarcinoma and normal cell lines. JF - PHARMACEUTICAL BIOLOGY J2 - PHARM BIOL VL - 60 PY - 2022 IS - 1 SP - 1511 EP - 1519 PG - 9 SN - 1388-0209 DO - 10.1080/13880209.2022.2103574 UR - https://m2.mtmt.hu/api/publication/33049439 ID - 33049439 AB - Ambrosia artemisiifolia L. (Asteraceae) contains sesquiterpene lactones as characteristic secondary metabolites. Many of these compounds exert antiproliferative and cytotoxic effects.To isolate the sesquiterpene lactones from the aerial part of A. artemisiifolia and to elucidate their cytotoxic, antiproliferative and antibacterial effects.The compounds were identified by one-dimensional (1D) and 2D NMR, HR-MS spectroscopy from the methanol extract. Isolated compounds were investigated for their cytotoxic and antiproliferative effects on human colonic adenocarcinoma cell lines and human embryonal lung fibroblast cell line using MTT assay. The selectivity of the sesquiterpenes was calculated towards the normal cell line. To check the effect of drug interactions between compounds and doxorubicin, multidrug-resistant Colo 320 cells were used.A new seco-psilostachyinolide derivative, 1,10-dihydro-1'-noraltamisin, and seven known compounds were isolated from the methanol extract. Acetoxydihydrodamsin had the most potent cytotoxic effect on sensitive (Colo205) cell line (IC50 = 7.64 µM), also the strongest antiproliferative effect on Colo205 (IC50 = 5.14 µM) and Colo320 (IC50 = 3.67 µM) cell lines. 1'-Noraltamisin (IC50 = 8.78 µM) and psilostachyin (IC50 = 5.29 µM) showed significant antiproliferative effects on the multidrug-resistant Colo320 cell line and had moderate selectivity against human embryonal lung fibroblast cell line. Psilostachyin C exhibited cytotoxic effects on Colo205 cells (IC50 = 26.60 µM). None of the isolated compounds inhibited ABCB1 efflux pump (EP; P-glycoprotein) or the bacterial EPs.Acetoxydihydrodamsin, 1'-noraltamisin, and psilostachyin showed the most remarkable cytotoxic and antiproliferative activity on tumour cell lines and exerted selectivity towards MRC-5 cell line. LA - English DB - MTMT ER - TY - JOUR AU - Saidu, Muhammad Bello AU - Kúsz, Norbert AU - Tsai, Yu-Chi AU - Vágvölgyi, Máté AU - Berkecz, Róbert AU - Kókai, Dávid AU - Burián, Katalin AU - Hohmann, Judit AU - Rédei, Dóra TI - Triterpenes and Phenolic Compounds from Euphorbia deightonii with Antiviral Activity against Herpes Simplex Virus Type-2 JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 11 PY - 2022 IS - 6 PG - 10 SN - 2223-7747 DO - 10.3390/plants11060764 UR - https://m2.mtmt.hu/api/publication/32765771 ID - 32765771 N1 - This research was funded by the Economic Development and Innovation Operative Programme (GINOP-2.3.2-15-2016-00012) and the National Research, Development and Innovation Fund (NKFI) under grant number K135845. M.B.S. was supported by the Stipendium Hungaricum scholarship program. AB - Two undescribed compounds, 3 beta,7 beta-dihydroxy-24-methylenelanosta-8-ene-11-one (1) and neolignane deightonin (4) were isolated from the aerial parts of Euphorbia deightonii Croizat together with six known compounds, namely, kansenone (2), euphorbol-7-one (3), dehydrodiconiferyl diacetate (5), marylaurencinol D (6), scoparon (7), and 3,4,3'-tri-O-methylellagic acid (8). The structures of the isolated compounds were determined by HRESIMS, 1D (H-1, C-13 JMOD) and 2D NMR (HSQC, HMBC, H-1-H-1 COSY, NOESY) spectroscopic analysis, and by comparison of the assignments with literature data. The anti-herpes simplex virus type-2 activity of the isolated compounds were investigated by qRT-PCR assay on Vero cells after determining cytotoxic concentration 50% (CC50). Compounds 1, 3, 4, and 7 exhibited inhibitory effects with respective IC50 values of 7.05, 2.42, 11.73, and 0.032 mu M. Scoparon (7) showed the strongest anti-HSV activity with a selectivity index of 10.93. LA - English DB - MTMT ER - TY - JOUR AU - Tsai, Yu-Chi AU - Nell, Racheal A. AU - Buckendorf, Jonathan E. AU - Kúsz, Norbert AU - Mwangi, Peter Waweru AU - Berkecz, Róbert AU - Rédei, Dóra AU - Vasas, Andrea AU - Spivak, Adam M. AU - Hohmann, Judit TI - Bioactive Compounds from Euphorbia usambarica Pax. with HIV-1 Latency Reversal Activity JF - PHARMACEUTICALS J2 - PHARMACEUTICALS-BASE VL - 14 PY - 2021 IS - 7 PG - 22 SN - 1424-8247 DO - 10.3390/ph14070653 UR - https://m2.mtmt.hu/api/publication/32100393 ID - 32100393 N1 - Cited By :1 Export Date: 9 August 2022 LA - English DB - MTMT ER - TY - JOUR AU - Mottaghipisheh, Javad AU - Kúsz, Norbert AU - Hohmann, Judit AU - Tsai, Yu-Chi AU - Csupor, Dezső TI - Isolation of secondary metabolites from the Iranian medicinal plant Eremurus persicus JF - ZEITSCHRIFT FÜR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES J2 - Z NATURFORSCH C VL - 76 PY - 2021 IS - 1-2 SP - 43 EP - 47 PG - 5 SN - 0939-5075 DO - 10.1515/znc-2020-0059 UR - https://m2.mtmt.hu/api/publication/31388948 ID - 31388948 LA - English DB - MTMT ER - TY - JOUR AU - Tsai, Yu-Chi AU - Rédei, Dóra AU - Hohmann, Judit AU - Wu, Chin-Chung TI - 12-Deoxyphorbol Esters Induce Growth Arrest and Apoptosis in Human Lung Cancer A549 Cells via Activation of PKC-δ/PKD/ERK Signaling Pathway JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 20 PG - 12 SN - 1661-6596 DO - 10.3390/ijms21207579 UR - https://m2.mtmt.hu/api/publication/31629692 ID - 31629692 LA - English DB - MTMT ER - TY - JOUR AU - Tsai, Yu-Chi AU - Hohmann, Judit AU - El-Shazly, Mohamed AU - Chang, Li-Kwan AU - Dankó, Balázs Ödön AU - Kúsz, Norbert AU - Hsieh, Chi-Ting AU - Hunyadi, Attila AU - Chang, Fang-Rong TI - Bioactive constituents of Lindernia crustacea and its anti-EBV effect via Rta expression inhibition in the viral lytic cycle JF - JOURNAL OF ETHNOPHARMACOLOGY J2 - J ETHNOPHARMACOL VL - 250 PY - 2020 PG - 7 SN - 0378-8741 DO - 10.1016/j.jep.2019.112493 UR - https://m2.mtmt.hu/api/publication/31192304 ID - 31192304 AB - Ethnopharmacological relevance Lindernia crustacea (L.) F.Muell. (Scrophulariaceae) was selected for phytochemical investigation owing to its traditional use against human herpes virus infection and its anti-Epstein–Barr virus (EBV) effect. Aims of the study The present study focused on the phytochemical investigation of L. crustacea including the isolation and structure determination of its biologically active compounds. Compounds with anti-EBV effects were also investigated. Materials and methods The EtOH extract of L. crustacea was subsequently partitioned using different solvents. The EtOAc fraction was subjected to several chromatographic methods to obtain pure compounds. The structures of all isolates were established by spectroscopic analysis and compared with previously reported physical data. The anti-EBV effect was evaluated in an EBV-containing Burkitt's lymphoma cell line (P3HR1) to study the expression of EBV lytic proteins. Results Thirty-three compounds, including one diterpene (1), four anthraquinones (2–5), two ionones (6 and 7), fourteen phenylpropanoid glycosides (8–21), five flavonoids (22–26), one lignan glycoside (27), one phenethyl alcohol glycoside (28), one phenylpropene glycoside (29), one glucosyl glycerol derivative (30), one furanone (31), and two cinnamic acid derivatives (32 and 33), were isolated from the ethanolic extract of the plant. All isolated compounds were obtained for the first time from Lindernia sp. The evaluation of the anti-EBV activity of L. crustacea crude extract, partitioned fractions, and constituents was performed for the first time. Phytol (1), aloe-emodin (2), byzantionoside B (7), a mixture of trans-martynoside (8) and cis-martynoside (9), a mixture of trans-isomartynoside (10) and cis-isomartynoside (11), luteolin-7-O-β-D-glucopyranoside (24), and apigenin-7-O-[β-D-apiofuranosyl (1→6)-β-D-glucopyranoside] (25) exhibited significant inhibitory effects on the EBV lytic cycle at 20 μg/mL in the immunoblot analysis. On the other hand, (6R,7E,9R)-3-oxo-α-ionol-β-D-glucopyranoside (6) and a mixture of trans-dolichandroside A (12) and cis-dolichandroside A (13) showed moderate anti-EBV activity at 20 μg/mL. Conclusions L. crustacea and its active isolates could be developed as potential candidates against EBV. Our findings provide scientific evidence for the traditional use of L. crustacea for its antiviral effects. LA - English DB - MTMT ER - TY - CHAP AU - Mottaghipisheh, Javad AU - Kúsz, Norbert AU - Hohmann, Judit AU - Tsai, Yu-Chi AU - Csupor, Dezső ED - Hohmann, Judit ED - Kiss, Tivadar ED - Csupor, Dezső TI - Isolation and structural elucidation of secondary metabolites from Eremurus persicus T2 - Trends in Natural Product Research - PSE Young Scientists’ Meeting on Biochemistry, Molecular Aspects and Pharmacology of Bioactive Natural Products PB - Szegedi Tudományegyetem (SZTE) CY - Szeged CY - Leicester PY - 2019 SP - 104 EP - 104 PG - 1 DO - 10.14232/tnpr.2019.po22 UR - https://m2.mtmt.hu/api/publication/30718583 ID - 30718583 LA - English DB - MTMT ER - TY - CHAP AU - Kovács, Balázs AU - Kiss, Tivadar AU - Csupor-Löffler, Boglárka AU - Ferencz, Elek AU - Kúsz, Norbert AU - Tsai, Yu-Chi AU - Hohmann, Judit AU - Csupor, Dezső ED - Hohmann, Judit ED - Kiss, Tivadar ED - Csupor, Dezső TI - Isolation of sesquiterpene lactones from common ragweed (Ambrosia artemisiifolia L.) T2 - Trends in Natural Product Research - PSE Young Scientists’ Meeting on Biochemistry, Molecular Aspects and Pharmacology of Bioactive Natural Products PB - Szegedi Tudományegyetem (SZTE) CY - Szeged CY - Leicester PY - 2019 SP - 103 EP - 103 PG - 1 DO - 10.14232/tnpr.2019.po21 UR - https://m2.mtmt.hu/api/publication/30718576 ID - 30718576 AB - Common ragweed (Ambrosia artemisiifolia L., Asteraceae) is an invasive species in Europe with allergic pollens. Ragweed originates from North America, but it also occurs and is spreading in Europe, due to the plant’s successful reproductive potential and a strong allelopathic effects against the native flora. Some of the plant’s secondary metabolites, called sesquiterpene lactones possess allergic, allopathic, anti-inflammatory, antitumor, antimicrobial activity. In a phytochemical view the plant secondary metabolites have not explored completely. The aim of our study was to isolate the major phytochemicals from the aerial parts of Ambrosia artemisiifolia, especially focusing on the sesquiterpene lactone compounds, and elucidate their chemical structure. The methanolic extract of the plant was separated by several chromatographic techniques, including preparative TLC and HPLC analysis. One new and six known sesquiterpene lactones were isolated from the aerial parts of ragweed. Their structure was identified by 1H NMR, 13C NMR and mass spectroscopy. 1’-Noraltamisin, a seco-pseudoguaianolide was reported for the first time from this plant. Further investigation needed to identify the biological activities and molecular mechanisms of the isolated compounds, particularly the new seco-pseudoguaianolide. LA - English DB - MTMT ER - TY - JOUR AU - Cheng, Y.-B. AU - Hu, H.-C. AU - Tsai, Yu-Chi AU - Chen, S.-L. AU - El-Shazly, M. AU - Nonato, M.G. AU - Wu, Y.-C. AU - Chang, F.-R. TI - Isolation and absolute configuration determination of alkaloids from Pandanus amaryllifolius JF - TETRAHEDRON J2 - TETRAHEDRON VL - 73 PY - 2017 IS - 25 SP - 3423 EP - 3429 PG - 7 SN - 0040-4020 DO - 10.1016/j.tet.2017.05.002 UR - https://m2.mtmt.hu/api/publication/30436568 ID - 30436568 N1 - Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan Research Center for Natural Products & Drug Development, Kaohsiung Medical University, Kaohsiung, 807, Taiwan Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Cairo, 115, Egypt Research Center for the Natural and Applied Sciences, University of Santo Tomas, Espana, Manila, 101, Philippines Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan Cited By :2 Export Date: 15 February 2019 CODEN: TETRA Correspondence Address: Wu, Y.-C.; Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical UniversityTaiwan; email: yachwu@kmu.edu.tw Funding details: Kaohsiung Medical University, KMU, KMU-TP103H02 Funding details: 103-2325-B-039-008 Funding details: 102-2320-B-037-012-MY2 Funding details: 103-2911-I-002-303 Funding details: 103-2325-B-039-007-CC1 Funding details: 105-2911-I-002-302 Funding details: Ministry of Health and Family Welfare, MOHFW, MOHW105-TDU-B-212-134007 Funding details: Ministry of Science and Technology, Taiwan, MOST Funding details: 102-2628-B-037-003-MY3 Funding details: National Health Research Institutes, NHRI, NHRI-EX103-10241BI Funding details: 103-2320-B-037-005-MY2 Funding text 1: This work was supported by grants from Ministry of Science?and Technology of Taiwan (MOST 105-2911-I-002-302, 103-2911-I-002-303, 103-2325-B-039-008, 103-2325-B-039-007-CC1, and 102-2320-B-037-012-MY2, awarded to Y.-C.W.; NSC 102-2628-B-037-003-MY3, MOST 103-2320-B-037-005-MY2, awarded to F.-R.C.; NSC 102-2320-B-037-012-MY2 award to Y.-B?C.), National Health Research Institutes (NHRI-EX103-10241BI), Kaohsiung Medical University (KMU-TP103H02), Ministry of Health and Welfare of Taiwan (MOHW105-TDU-B-212-134007), and health and welfare surcharge of tobacco products. Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan Research Center for Natural Products & Drug Development, Kaohsiung Medical University, Kaohsiung, 807, Taiwan Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Cairo, 115, Egypt Research Center for the Natural and Applied Sciences, University of Santo Tomas, Espana, Manila, 101, Philippines Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan Cited By :2 Export Date: 17 February 2019 CODEN: TETRA Correspondence Address: Wu, Y.-C.; Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical UniversityTaiwan; email: yachwu@kmu.edu.tw Funding details: Kaohsiung Medical University, KMU, KMU-TP103H02 Funding details: 103-2325-B-039-008 Funding details: 102-2320-B-037-012-MY2 Funding details: 103-2911-I-002-303 Funding details: 103-2325-B-039-007-CC1 Funding details: 105-2911-I-002-302 Funding details: Ministry of Health and Family Welfare, MOHFW, MOHW105-TDU-B-212-134007 Funding details: Ministry of Science and Technology, Taiwan, MOST Funding details: 102-2628-B-037-003-MY3 Funding details: National Health Research Institutes, NHRI, NHRI-EX103-10241BI Funding details: 103-2320-B-037-005-MY2 Funding text 1: This work was supported by grants from Ministry of Science?and Technology of Taiwan (MOST 105-2911-I-002-302, 103-2911-I-002-303, 103-2325-B-039-008, 103-2325-B-039-007-CC1, and 102-2320-B-037-012-MY2, awarded to Y.-C.W.; NSC 102-2628-B-037-003-MY3, MOST 103-2320-B-037-005-MY2, awarded to F.-R.C.; NSC 102-2320-B-037-012-MY2 award to Y.-B?C.), National Health Research Institutes (NHRI-EX103-10241BI), Kaohsiung Medical University (KMU-TP103H02), Ministry of Health and Welfare of Taiwan (MOHW105-TDU-B-212-134007), and health and welfare surcharge of tobacco products. AB - Seven new alkaloids belonging to three typical classes, isolated from an ethanolic extract of Pandanus amaryllifolius, were applied to establish the models in structural elucidation. The first type [pandanusines A and B (1 and 2)] was applied to alkaloids with a diazaspirocyclic ring system. The absolute stereochemistry of aminal carbon is determined by analysis of J-based configuration analysis and circular dichroism spectroscopy, which has never been revealed before. The second type [pandalizines C–E (3–5)], the stereochemistry of unusual indolizinone with chiral hydroxyl substitutions were determined by circular dichroism spectroscopy and vibrational circular dichroism experiments. The last type [norpandamarilactonines C and D (6 and 7)], a comparing model in determination of diastereomers of pyrrolidinyl α,β-unsaturated γ-lactone alkaloids was established. Our study provides solutions for a challenging subject in stereochemistry determination for three different types of Pandanus alkaloids. © 2017 Elsevier Ltd LA - English DB - MTMT ER -