TY - THES AU - Hinsenkamp, Adél Gyöngyvér TI - The Use of Human Blood Derived Protein Components and Crosslinked Hyaluronic Acid in Tissue Engineering and Regenerative Medicine PY - 2022 DO - 10.14753/SE.2022.2615 UR - https://m2.mtmt.hu/api/publication/33720623 ID - 33720623 LA - English DB - MTMT ER - TY - CONF AU - Majer, Aliz AU - Julianna, Pesthy AU - Besztercei, Balázs AU - Hinsenkamp, Adél Gyöngyvér AU - László, Smellér AU - Lacza, Zsombor AU - Benyó, Zoltán AU - Hornyák, István AU - Ruisanchez, Éva TI - Characterization of Native and Human Serum Albumin-bound Lysophosphaditic Acid Species and their Effect on the Viability of Mesenchymal Stem Cells In vitro T2 - Bioactive Lipids in Cancer, Inflammation and Related Diseases PY - 2022 SP - & UR - https://m2.mtmt.hu/api/publication/33597990 ID - 33597990 LA - English DB - MTMT ER - TY - PAT AU - Lacza, Zsombor AU - Hornyák, István AU - Hinsenkamp, Adél Gyöngyvér TI - CROSS-LINKED HYALURONIC ACID HYDROGELS COMPRISING PROTEINS CY - Country:10017(13) PY - 2022 PG - 32 UR - https://m2.mtmt.hu/api/publication/33119670 ID - 33119670 LA - English DB - MTMT ER - TY - JOUR AU - Hinsenkamp, Adél Gyöngyvér AU - Fülöp, Ágnes AU - Hricisák, László AU - Pál, Éva AU - Kun, Kiara AU - Majer, Aliz AU - Varga, Viktória Dóra AU - Lacza, Zsombor AU - Hornyák, István TI - Application of Injectable, Crosslinked, Fibrin-Containing Hyaluronic Acid Scaffolds for In Vivo Remodeling JF - JOURNAL OF FUNCTIONAL BIOMATERIALS J2 - J FUNCT BIOMATER VL - 13 PY - 2022 IS - 3 PG - 16 SN - 2079-4983 DO - 10.3390/jfb13030119 UR - https://m2.mtmt.hu/api/publication/33054906 ID - 33054906 AB - The present research aimed to characterize soft tissue implants that were prepared with the use of crosslinked hyaluronic acid (HA) using two different crosslinkers and multiple reagent concentrations, alone or in combination with fibrin. The effect of the implants was evaluated in an in vivo mouse model, after 4 weeks in one group and after 12 weeks in the other. The explants were compared using analytical methods, evaluating microscopic images, and a histology analysis. The kinetics of the degradation and remodeling of explants were found to be greatly dependent on the concentration and type of crosslinker; generally, divinyl sulfone (DVS) resists degradation more effectively compared to butanediol diglycidyl ether (BDDE). The presence of fibrin enhances the formation of blood vessels, and the infiltration of cells and extracellular matrix. In summary, if the aim is to create a soft tissue implant with easier degradation of the HA content, then the use of 2–5% BDDE is found to be optimal. For a longer degradation time, 5% DVS is the more suitable crosslinker. The use of fibrin was found to support the biological process of remodeling, while keeping the advances of HA in void filling, enabling the parallel degradation and remodeling processes. LA - English DB - MTMT ER - TY - JOUR AU - Majer, Aliz AU - Pesthy, Julianna AU - Besztercei, Balázs AU - Hinsenkamp, Adél Gyöngyvér AU - Smeller, László AU - Lacza, Zsombor AU - Benyó, Zoltán AU - Ruisanchez, Éva AU - Hornyák, István TI - Characterization of Native and Human Serum Albumin-Bound Lysophosphatidic Acid Species and Their Effect on the Viability of Mesenchymal Stem Cells In Vitro JF - APPLIED SCIENCES-BASEL J2 - APPL SCI-BASEL VL - 12 PY - 2022 IS - 16 PG - 10 SN - 2076-3417 DO - 10.3390/app12168183 UR - https://m2.mtmt.hu/api/publication/33054705 ID - 33054705 AB - Scaffolds can provide a healthy environment for cell attachment, differentiation, proliferation, and migration in vitro and in vivo. Lysophosphatidic acid (LPA) is a naturally occurring bioactive phospholipid that is present in the serum mainly bound to albumin. The present study aims to investigate the biocompatibility of LPA. It also aims to determine the effect of different LPA species on the proliferation and migration of human bone marrow-derived mesenchymal stem cells (hBM-dMSCs) for LPA and human serum albumin (HSA) containing bone scaffold development. The HSA-LPA complex formation was assessed using Fourier-transform infrared (FTIR) spectroscopy. The effect of 18:1, 18:2, or 16:0 LPA alone, or in combination with 4% HSA, on cell viability and proliferation was determined by XTT. The cell migration was examined in a wound healing assay. The changes in the FTIR spectra of LPA-HSA compositions, compared with HSA alone, indicate the complex formation between the components. Our study showed that 18:1, 18:2, and 16:0 LPA species had no cytotoxic effects up to 10 µM concentration. The different LPA species increased the proliferation of hBM-dMSCs in a dose-dependent manner when administered in the presence of HSA, without an effect on the migration of this cell type. These findings make the in vivo application of LPA-HSA complex promising for bone regeneration. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, Eszter AU - Olmos Calvo, I AU - Kuten-Pella, O AU - Hamar, E AU - Bukva, Mátyás AU - Madár, Á AU - Hornyák, István AU - Hinsenkamp, Adél Gyöngyvér AU - Hetényi, Roland AU - Földes, Fanni Vivien AU - Zana, Brigitta AU - Jakab, Ferenc AU - Kemenesi, Gábor AU - Lacza, Zsombor TI - Comparison of immune activation of the COVID vaccines. ChAdOx1, BNT162b2, mRNA-1273, BBIBP-CorV, and Gam-COVID-Vac from serological human samples in Hungary showed higher protection after mRNA-based immunization. TS - ChAdOx1, BNT162b2, mRNA-1273, BBIBP-CorV, and Gam-COVID-Vac from serological human samples in Hungary showed higher protection after mRNA-based immunization. JF - EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES J2 - EUR REV MED PHARMACOL SCI VL - 26 PY - 2022 IS - 14 SP - 5297 EP - 5306 PG - 10 SN - 1128-3602 DO - 10.26355/eurrev_202207_29321 UR - https://m2.mtmt.hu/api/publication/33041654 ID - 33041654 AB - To gain insight into the different protective mechanisms of approved vaccines, this study focuses on the comparison of humoral and cellular immune responses of five widely used vaccines including ChAdOx1 (AZD1222, AstraZeneca), BNT162b2 (Pfizer), mRNA-1273 (Moderna), BBIBP-CorV (Sinopharm), and Gam-COVID-Vac (Sputnik V).Isolated plasma from 95 volunteers' blood samples was used to measure anti-SARS-CoV-2 humoral and cellular immune responses. Positive controls were recovered patients from COVID-19 (unvaccinated). Specific quantification kits for anti-nucleocapsid IgG, anti-Spike protein IgG, neutralizing antibodies as well as specific SARS-CoV-2 antigens for T-cell activation were used and Spearman correlation and matrix analyses were performed to compare overall immune responses.Nucleocapsid antibodies were significantly higher for the BBIBP-CorV and convalescent group when compared to other vaccines. In contrast, subjects vaccinated with BNT162b2 and mRNA-1273 presented significantly higher anti-spike IgG. In fact, 9.1% of convalescent, 4.5% of Gam-COVID-Vac, 28.6% of ChAdOx1, and 12.5% of BBIBP-CorV volunteers did not generate anti-spike IgG. Similarly, a positive correlation was observed after the neutralization assay. T-cell activation studies showed that mRNA-based vaccines induced a T-cell driven immune response in all cases, while 55% of convalescents, 8% of BNT162b1, 12,5% of mRNA-1273, 9% of Gam-COVID-Vac, 57% of ChAdOx1, and 56% of BBIBP-CorV subjects presented no cellular response. Further correlation matrix analyses indicated that anti-spike IgG and neutralizing antibodies production, and T-cell activation follow the same trend after immunization.RNA-based vaccines induced the most robust adaptive immune activation against SARS-CoV-2 by promoting a significantly higher T-cell response, anti-spike IgG and neutralization levels. Vector-based vaccines protected against the virus at a comparable level to convalescent patients. LA - English DB - MTMT ER - TY - JOUR AU - Hinsenkamp, Adél Gyöngyvér AU - Benyó, Zoltán AU - Hornyák, István TI - Overview of tissue engineering patent strategies and patents from 2010 to 2020 including outcomes JF - TISSUE ENGINEERING PART B REVIEWS J2 - TISSUE ENG PART B REV VL - 28 PY - 2022 IS - 3 SP - 626 EP - 632 PG - 8 SN - 1937-3368 DO - 10.1089/ten.TEB.2021.0045 UR - https://m2.mtmt.hu/api/publication/32108482 ID - 32108482 N1 - Ellenőrizve teljes szöveg alapján GT LA - English DB - MTMT ER - TY - CONF AU - Hornyák, István AU - Hinsenkamp, Adél Gyöngyvér AU - Lacza, Zsombor TI - CROSSLINKED HYALURONIC ACID GELS WITH BLOOD-DERIVED PROTEIN COMPONENTS FOR SOFT TISSUE REGENERATION T2 - Books of Abstract, 6th World TERMIS Conference PY - 2021 SP - 352 EP - 352 PG - 1 UR - https://m2.mtmt.hu/api/publication/32517623 ID - 32517623 AB - Abstract #408 LA - English DB - MTMT ER - TY - CONF AU - Isabel, Olmos Calvo AU - Olga, Kuten-Pella AU - Hornyák, István AU - Hinsenkamp, Adél Gyöngyvér AU - Andrea, de Luna AU - Karina, Kramer AU - Stefan, Nehrer AU - Lacza, Zsombor TI - HYPERACUTE SERUM AS A REGENERATIVE THERAPEUTIC IN IN VITRO MODELS OF OSTEOARTHRITIS T2 - Books of Abstract, 6th World TERMIS Conference PY - 2021 SP - 763 EP - 763 PG - 1 UR - https://m2.mtmt.hu/api/publication/32516963 ID - 32516963 LA - English DB - MTMT ER - TY - JOUR AU - Hinsenkamp, Adél Gyöngyvér AU - Kun, Kiara AU - Gajnut, Fatime AU - Majer, Aliz AU - Lacza, Zsombor AU - Hornyák, István TI - Cell Attachment Capacity and Compounds of Fibrin Membranes Isolated from Fresh Frozen Plasma and Cryoprecipitate JF - MEMBRANES (BASEL) J2 - MEMBRANES-BASEL VL - 11 PY - 2021 IS - 10 PG - 13 SN - 2077-0375 DO - 10.3390/membranes11100783 UR - https://m2.mtmt.hu/api/publication/32369394 ID - 32369394 AB - Fibrin membranes are widely used in regenerative medicine because they are biocompatible, biodegradable, contain growth factors, and support cell attachment. Most commonly they are produced from serum, but they can also be isolated from activated plasma. To increase the fibrinogen concentration of plasma, cryoprecipitate isolation is a possible solution. In this work, cryoprecipitate was prepared from fresh frozen plasma, isolated by plasmapheresis. The concentration of cellular elements, fibrinogen, total protein, and immunoglobulins among others was measured in different concentrations of cryoprecipitates. After activation with Ca-gluconate, fibrin membranes were produced in different thicknesses, and human mesenchymal stem cells were seeded onto the membranes. They were visualized by live-dead staining and their viability was determined by XTT. The platelet-derived growth factor AB content was quantified by ELISA. Our results showed that fibrinogen and platelet concentration can be multiplied in plasma by cryoprecipitate isolation, which affects the thickness and slightly the growth factor content of the membranes. According to live-dead staining, the thickness of the membranes does not influence cell attachment, and XTT measurement did not reveal a significant difference in cell attachment capacity either; however, a growing trend could be observed in the case of some membranes. LA - English DB - MTMT ER -