TY - JOUR AU - Barabási, Beáta AU - Barna, Lilla AU - Santa Maria, Anaraquel AU - Harazin, András AU - Molnár, Réka AU - Kincses, András AU - Vigh, Judit Piroska AU - Dukay, Brigitta AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda AU - Walter, Fruzsina AU - Deli, Mária Anna AU - Hoyk, Zsófia TI - Role of interleukin-6 and interleukin-10 in morphological and functional changes of the blood–brain barrier in hypertriglyceridemia JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 20 PY - 2023 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-023-00418-3 UR - https://m2.mtmt.hu/api/publication/33688118 ID - 33688118 N1 - Funding Agency and Grant Number: ELKH Biological Research Center - National Research, Development, and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00060]; European Training Network [H2020-MSCA-ITN-2015, 675619] Funding text: Open access funding provided by ELKH Biological Research Center. This work was funded by the National Research, Development, and Innovation Office of Hungary, Grant Number GINOP-2.3.2-15-2016-00060. ARSM was supported by the European Training Network H2020-MSCA-ITN-2015 [Grant Number 675619]. LA - English DB - MTMT ER - TY - JOUR AU - Dukay, Brigitta AU - Walter, Fruzsina AU - Vigh, Judit Piroska AU - Barabási, Beáta AU - Hajdu, Petra AU - Balassa, Tamás AU - Migh, Ede AU - Kincses, András AU - Hoyk, Zsófia AU - Szögi, Titanilla AU - Borbély, Emőke AU - Csoboz, Bálint AU - Horváth, Péter AU - Fülöp, Lívia AU - Penke, Botond AU - Vigh, László AU - Deli, Mária Anna AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda TI - Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury JF - JOURNAL OF NEUROINFLAMMATION J2 - J NEUROINFLAMM VL - 18 PY - 2021 IS - 1 PG - 24 SN - 1742-2094 DO - 10.1186/s12974-020-02070-2 UR - https://m2.mtmt.hu/api/publication/31807147 ID - 31807147 N1 - Miklós Sántha and Melinda E. Tóth are shared last authors. LA - English DB - MTMT ER - TY - GEN AU - Barabási, Beáta AU - Barna, L AU - Harazin, A AU - Toth, M AU - Dukay, B AU - Santha, M AU - Hoyk, Z AU - Deli, M TI - IL-6 and IL-10 induced changes in brain endothelium in a mouse model of human atherosclerosis PY - 2019 UR - https://m2.mtmt.hu/api/publication/31432297 ID - 31432297 LA - English DB - MTMT ER - TY - GEN AU - Hoyk, Z AU - Barabási, Beáta AU - Barna, L AU - Harazin, A AU - Vigh, J AU - Toth, M AU - Dukay, B AU - Santha, M TI - P-glycoprotein expressional changes in brain microvessel endothelial cells related to neuroinflammation PY - 2018 UR - https://m2.mtmt.hu/api/publication/31432611 ID - 31432611 LA - English DB - MTMT ER - TY - GEN AU - Barabási, Beáta AU - Barna, L AU - Harazin, A AU - Vigh, J AU - Toth, M AU - Dukay, B AU - Santha, M AU - Hoyk, Z AU - Deli, MA TI - P-glycoprotein expressional changes in brain capillary endothelial cells related to neuroinflammation PY - 2018 UR - https://m2.mtmt.hu/api/publication/30725306 ID - 30725306 N1 - poster LA - English DB - MTMT ER - TY - JOUR AU - Hoyk, Zsófia AU - Tóth, Erzsébet Melinda AU - Lénárt, Nikolett AU - Nagy, Dóra AU - Dukay, Brigitta AU - Csefová, Alexandra AU - Zvara, Ágnes AU - Seprényi, György AU - Kincses, András AU - Walter, Fruzsina AU - Veszelka, Szilvia AU - Vigh, Judit Piroska AU - Barabási, Beáta AU - Harazin, András AU - Kittel, Ágnes AU - Puskás, László AU - Penke, Botond AU - Vigh, László AU - Deli, Mária Anna AU - Sántha, Miklós TI - Cerebrovascular Pathology in Hypertriglyceridemic APOB-100 Transgenic Mice JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 12 ET - 0 PY - 2018 PG - 17 SN - 1662-5102 DO - 10.3389/fncel.2018.00380 UR - https://m2.mtmt.hu/api/publication/30312141 ID - 30312141 N1 - These authors have contributed equally to this work: Zsófia Hoyk, Melinda E. Tóth. Funding text: This work was supported by funding from National Research, Development and Innovation Office, Hungary (GINOP2.3.2.-15.2016-00060) and the Hungarian Basic Research Fund (OTKA NN111006). AB - Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressing the human APOB-100 protein, a mouse model of human atherosclerosis. In this model we observed high plasma levels of triglycerides, oxidative stress, tau hyperphosphorylation, synaptic dysfunction, cognitive impairment, increased neural apoptosis and neurodegeneration. Neurovascular dysfunction is recognized as a key factor in the development of neurodegenerative diseases, but the cellular and molecular events linking cerebrovascular pathology and neurodegeneration are not fully understood. Our aim was to study cerebrovascular changes in APOB-100 transgenic mice. We described the kinetics of the development of chronic hypertriglyceridemia in the transgenic animals. Increased blood-brain barrier permeability was found in the hippocampus of APOB-100 transgenic mice which was accompanied by structural changes. Using transmission electron microscopy, we detected changes in the brain capillary endothelial tight junction structure and edematous swelling of astrocyte endfeet. In brain microvessels isolated from APOB-100 transgenic animals increased Lox-1, Aqp4, and decreased Meox-2, Mfsd2a, Abcb1a, Lrp2, Glut-1, Nos2, Nos3, Vim, and in transgenic brains reduced Cdh2 and Gfap-σ gene expressions were measured using quantitative real-time PCR. We confirmed the decreased P-glycoprotein (ABCB1) and vimentin expression related to the neurovascular unit by immunostaining in transgenic brain sections using confocal microscopy. We conclude that in chronic hypertriglyceridemic APOB-100 transgenic mice both functional and morphological cerebrovascular pathology can be observed, and this animal model could be a useful tool to study the link between cerebrovascular pathology and neurodegeneration. LA - English DB - MTMT ER - TY - JOUR AU - Barabási, Beáta AU - Csondor, A AU - Martin-Pozas, T AU - Sanchez, AM AU - Antalffy, G AU - Siklós, László AU - Gomez-Pinedo, U AU - Párducz, Árpád AU - Hoyk, Zsófia TI - Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice. JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 319 PY - 2016 SP - 107 EP - 115 PG - 9 SN - 0306-4522 DO - 10.1016/j.neuroscience.2016.01.049 UR - https://m2.mtmt.hu/api/publication/3013488 ID - 3013488 N1 - Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 26 March 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 28 April 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. AB - The objective of the study was to examine whether axotomy and 17beta-estradiol affects P2X7 receptor expression and distribution in the hypoglossal nucleus. The left hypoglossal nerve of ovariectomized mice was cut and animals received a single injection of 17beta-estradiol (25mug/100 g b. w. in 20% (2- hydroxypropyl) - beta- cyclodextrin) or vehicle one hour after axotomy. Mice were sacrificed on day 4 following surgery. The area fraction of P2X7 receptor immunoreactive structures and of CD11b immunolabeled microglia, P2X7 protein concentration, and the immunoreactivity pattern of estrogen receptor alpha / beta were analyzed on both sides of the hypoglossal nucleus. Following axotomy the area fraction of P2X7 immunoreactive neurons showed a decreasing tendency, while the area fraction of P2X7 immunolabeled microglia increased significantly on the axotomized side compared with the control side in mice injected with vehicle. In animals treated with 17beta-estradiol the decrease in area fraction of neural and the increase in area fraction of microglial P2X7 immunostaining on the axotomized side were significantly enhanced compared with animals injected with vehicle. The P2X7 immunoreactivity pattern on the control side of the nucleus remained unchanged after 17beta-estradiol injection. Semi-quantitative Western blots revealed no significant difference in P2X7 protein concentration comparing the axotomized side with the control side in either experimental group. The CD11b immunoreactive microglia area fraction increased significantly following axotomy, but was not affected by 17beta-estradiol. Neither estrogen receptor alpha, nor beta colocalized with CD11b. Our results suggest that axotomy induces cell-type specific changes in P2X7 receptor expression, which may be directly regulated by 17beta-estradiol through estrogen receptor alpha or beta in neurons, but not in activated microglia. LA - English DB - MTMT ER - TY - GEN AU - Hoyk, Z AU - Barabási, Beáta AU - Csondor, A AU - Martin-Pozas, T AU - Pulupa, Sanchez AM AU - Siklos, L AU - Gomez-Pinedo, U AU - Parducz, A TI - 17β-estradiol augments axotomy-induced cell-type specific changes in P2X7 receptor expression in the mouse hypoglossal nucleus PY - 2015 UR - https://m2.mtmt.hu/api/publication/30725313 ID - 30725313 N1 - poster LA - English DB - MTMT ER - TY - JOUR AU - Hoyk, Zsófia AU - Barabási, Beáta AU - Csondor, A AU - Martin-Pozas, T AU - Sanchez, AMP AU - Siklos, L AU - Gomez-Pinedo, U AU - Parducz, A TI - 17 beta-estradiol augments axotomy-induced cell-type specific changes in P2X7 receptor expression in the mouse hypoglossal nucleus JF - GLIA J2 - GLIA VL - 63 PY - 2015 IS - 1 SP - E419 EP - E419 PG - 1 SN - 0894-1491 UR - https://m2.mtmt.hu/api/publication/2921753 ID - 2921753 N1 - SU 1 LA - English DB - MTMT ER - TY - GEN AU - Csondor, A AU - Barabási, Beáta AU - Siklos, L AU - Parducz, A AU - Hoyk, Z TI - Estrogen effect on the immune functions of microglia in the oculomotor and hypoglossal nucleus of mice following peripheral axotomy PY - 2014 UR - https://m2.mtmt.hu/api/publication/30725323 ID - 30725323 N1 - poster LA - English DB - MTMT ER -