TY - JOUR AU - Ghani, Marvi AU - Zohar, Peleg AU - Ujlaki, Gyula AU - Tóth, Melinda AU - Amsalu, Hailemariam AU - Póliska, Szilárd AU - Tar, Krisztina TI - Stable knockdown of Drp1 improves retinoic acid-BDNF-induced neuronal differentiation through global transcriptomic changes and results in reduced phosphorylation of ERK1/2 independently of DUSP1 and 6 JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 12 PY - 2024 SP - 1 EP - 25 PG - 25 SN - 2296-634X DO - 10.3389/fcell.2024.1342741 UR - https://m2.mtmt.hu/api/publication/34738061 ID - 34738061 AB - Background: Dynamin-related protein Drp1 —a major mitochondrial fission protein— is widely distributed in the central nervous system and plays a crucial role in regulating mitochondrial dynamics, specifically mitochondrial fission and the organelle's shaping. Upregulated Drp1 function may contribute to the pathological progression of neurodegenerative diseases by dysregulating mitochondrial fission/ fusion. The study aims to investigate the effects of Drp1 on retinoic acid-BDNF-induced (RA-BDNF) neuronal differentiation and mitochondrial network reorganization in SH-SY5Y neuroblastoma cells. LA - English DB - MTMT ER - TY - JOUR AU - Ujlaki, Gyula AU - Kovács, Tünde AU - Vida, András AU - Kókai, Endre AU - Rauch, Boglárka AU - Schwarcz, Szandra AU - Mikó, Edit AU - Janka, Eszter AU - Sipos, Adrienn AU - Hegedűs, Csaba AU - Uray (Davis), Karen L. AU - Nagy, Péter AU - Bay, Péter TI - Identification of Bacterial Metabolites Modulating Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 15 SN - 1420-3049 DO - 10.3390/molecules28155898 UR - https://m2.mtmt.hu/api/publication/34089348 ID - 34089348 AB - Breast cancer patients are characterized by the oncobiotic transformation of multiple microbiome communities, including the gut microbiome. Oncobiotic transformation of the gut microbiome impairs the production of antineoplastic bacterial metabolites. The goal of this study was to identify bacterial metabolites with antineoplastic properties. We constructed a 30-member bacterial metabolite library and screened the library compounds for effects on cell proliferation and epithelial-mesenchymal transition. The metabolites were applied to 4T1 murine breast cancer cells in concentrations corresponding to the reference serum concentrations. However, yric acid, glycolic acid, d-mannitol, 2,3-butanediol, and trans-ferulic acid exerted cytostatic effects, and 3-hydroxyphenylacetic acid, 4-hydroxybenzoic acid, and vanillic acid exerted hyperproliferative effects. Furthermore, 3-hydroxyphenylacetic acid, 4-hydroxybenzoic acid, 2,3-butanediol, and hydrocinnamic acid inhibited epithelial-to-mesenchymal (EMT) transition. We identified redox sets among the metabolites (d-mannitol—d-mannose, 1-butanol—butyric acid, ethylene glycol—glycolic acid—oxalic acid), wherein only one partner within the set (d-mannitol, butyric acid, glycolic acid) possessed bioactivity in our system, suggesting that changes to the local redox potential may affect the bacterial secretome. Of the nine bioactive metabolites, 2,3-butanediol was the only compound with both cytostatic and anti-EMT properties. LA - English DB - MTMT ER - TY - JOUR AU - Antal, Dóra AU - Pór, Ágnes AU - Kovács, Ilona AU - Dull, Katalin AU - Póliska, Szilárd AU - Ujlaki, Gyula AU - Demény, Máté Ágoston AU - Szöllősi, Attila AU - Kiss, Borbála AU - Szegedi, Andrea AU - Bay, Péter AU - Szántó, Magdolna TI - PARP2 promotes inflammation in psoriasis by modulating estradiol biosynthesis in keratinocytes JF - JOURNAL OF MOLECULAR MEDICINE-JMM J2 - J MOL MED-JMM VL - 101 PY - 2023 SP - 987 EP - 999 PG - 13 SN - 0946-2716 DO - 10.1007/s00109-023-02338-z UR - https://m2.mtmt.hu/api/publication/34034279 ID - 34034279 LA - English DB - MTMT ER - TY - JOUR AU - Skopál, Adrienn AU - Ujlaki, Gyula AU - Gerencsér, Attila Tibor AU - Bankó, Csaba AU - Bacsó, Zsolt AU - Ciruela, Francisco AU - Virág, László AU - Haskó, György AU - Kókai, Endre TI - Adenosine A2A Receptor Activation Regulates Niemann–Pick C1 Expression and Localization in Macrophages JF - CURRENT ISSUES IN MOLECULAR BIOLOGY J2 - CURR ISSUES MOL BIOL VL - 45 PY - 2023 IS - 6 SP - 4948 EP - 4969 PG - 22 SN - 1467-3037 DO - 10.3390/cimb45060315 UR - https://m2.mtmt.hu/api/publication/34020857 ID - 34020857 N1 - Funding Agency and Grant Number: National Research Development and Innovation Office [OTKA MB08A 84685, GINOP-2.3.2-15-2016-00020 TUMORDNS, R01GM066189]; National Research, Development and Innovation Office [K147482, GINOP-2.3.2-15-2016-00048-STAYALIVE]; National Institutes of Health [R01DK113790, R01HL158519]; [OTKA K132193] Funding text: EK received funding from National Research Development and Innovation Office OTKA MB08A 84685; LV received funding from the National Research, Development and Innovation Office grants GINOP-2.3.2-15-2016-00020 TUMORDNS, GINOP-2.3.2-15-2016-00048-STAYALIVE and National Research Development and Innovation Office OTKA K132193, K147482. GH received funding from National Institutes of Health grants R01GM066189, R01DK113790 and R01HL158519. AB - Adenosine plays an important role in modulating immune cell function, particularly T cells and myeloid cells, such as macrophages and dendritic cells. Cell surface adenosine A2A receptors (A2AR) regulate the production of pro-inflammatory cytokines and chemokines, as well as the proliferation, differentiation, and migration of immune cells. In the present study, we expanded the A2AR interactome and provided evidence for the interaction between the receptor and the Niemann–Pick type C intracellular cholesterol transporter 1 (NPC1) protein. The NPC1 protein was identified to interact with the C-terminal tail of A2AR in RAW 264.7 and IPMФ cells by two independent and parallel proteomic approaches. The interaction between the NPC1 protein and the full-length A2AR was further validated in HEK-293 cells that permanently express the receptor and RAW264.7 cells that endogenously express A2AR. A2AR activation reduces the expression of NPC1 mRNA and protein density in LPS-activated mouse IPMФ cells. Additionally, stimulation of A2AR negatively regulates the cell surface expression of NPC1 in LPS-stimulated macrophages. Furthermore, stimulation of A2AR also altered the density of lysosome-associated membrane protein 2 (LAMP2) and early endosome antigen 1 (EEA1), two endosomal markers associated with the NPC1 protein. Collectively, these results suggested a putative A2AR-mediated regulation of NPC1 protein function in macrophages, potentially relevant for the Niemann–Pick type C disease when mutations in NPC1 protein result in the accumulation of cholesterol and other lipids in lysosomes. LA - English DB - MTMT ER - TY - JOUR AU - Lénárt, Kinga AU - Kovács, Dóra AU - Demény, Máté Ágoston AU - Ujlaki, Gyula AU - Christos, Zouboulis AU - Szegedi, Andrea AU - Törőcsik, Dániel TI - Transglutaminase 2 deficiency leads to lipid accumulation and reduced autophagy in SZ95 sebocytes JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 143 PY - 2023 IS - 5 SP - S228 EP - S228 SN - 0022-202X UR - https://m2.mtmt.hu/api/publication/34003596 ID - 34003596 LA - English DB - MTMT ER - TY - CHAP AU - Antal, Dóra AU - Pór, Ágnes AU - Kovács, Ilona AU - Dull, Katalin AU - Póliska, Szilárd AU - Ujlaki, Gyula AU - Demény, Máté Ágoston AU - Szöllősi, A. G. AU - Kiss, B. AU - Szegedi, Andrea AU - Bay, Péter AU - Szántó, Magdolna TI - PARP2 promotes inflammation in psoriasis by modulating estradiol biosynthesis in keratinocytes . T2 - FEBS Advanced Lecture Course: PARP 2023: Research on the family of poly(ADP-ribose) polymerases; Book of Abstracts PB - Ruder Boskovic Institue CY - Hvar SN - 9789537941468 PY - 2023 SP - 60 UR - https://m2.mtmt.hu/api/publication/33998388 ID - 33998388 LA - English DB - MTMT ER - TY - JOUR AU - Schwarcz, Szandra AU - Kovács, Patrik Bence AU - Kovács, Tünde AU - Ujlaki, Gyula AU - Nyerges, P. AU - Uray (Davis), Karen L. AU - Bay, Péter AU - Mikó, Edit TI - The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models JF - MOLECULAR BIOLOGY REPORTS J2 - MOL BIOL REP VL - 50 PY - 2023 IS - 6 SP - 5273 EP - 5282 PG - 10 SN - 0301-4851 DO - 10.1007/s11033-023-08453-x UR - https://m2.mtmt.hu/api/publication/33764771 ID - 33764771 LA - English DB - MTMT ER - TY - JOUR AU - Lénárt, Kinga AU - Bankó, Csaba AU - Ujlaki, Gyula AU - Póliska, Szilárd AU - Kis, Nikoletta Gréta AU - Csősz, Éva AU - Antal, Miklós AU - Bacsó, Zsolt AU - Bay, Péter AU - Fésüs, László AU - Mádi, András TI - Tissue Transglutaminase Knock-Out Preadipocytes and Beige Cells of Epididymal Fat Origin Possess Decreased Mitochondrial Functions Required for Thermogenesis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 9 PG - 21 SN - 1661-6596 DO - 10.3390/ijms23095175 UR - https://m2.mtmt.hu/api/publication/32820433 ID - 32820433 N1 - 332794 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Tünde AU - Mikó, Edit AU - Ujlaki, Gyula AU - Yousef, H AU - Csontos, V AU - Uray (Davis), Karen L. AU - Bay, Péter TI - The involvement of oncobiosis and bacterial metabolite signaling in metastasis formation in breast cancer JF - CANCER AND METASTASIS REVIEWS J2 - CANCER METAST REV VL - 40 PY - 2021 IS - 4 SP - 1223 EP - 1249 PG - 27 SN - 0167-7659 DO - 10.1007/s10555-021-10013-3 UR - https://m2.mtmt.hu/api/publication/32540511 ID - 32540511 N1 - 327413 LA - English DB - MTMT ER - TY - JOUR AU - Kacsir, I AU - Sipos, Adrienn AU - Ujlaki, Gyula AU - Buglyó, Péter AU - Somsák, László AU - Bay, Péter AU - Bokor, Éva TI - Ruthenium half-sandwich type complexes with bidentate monosaccharide ligands show antineoplastic activity in ovarian cancer cell models through reactive oxygen species production JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 19 PG - 38 SN - 1661-6596 DO - 10.3390/ijms221910454 UR - https://m2.mtmt.hu/api/publication/32287783 ID - 32287783 N1 - Department of Organic Chemistry, University of Debrecen, P.O. Box 400, Debrecen, H-4002, Hungary Doctoral School of Chemistry, University of Debrecen, P.O. Box 400, Debrecen, H-4002, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Inorganic & Analytical Chemistry, Faculty of Sciences and Technology, University of Debrecen, Debrecen, H-4032, Hungary NKFIH-DE Lendület Laboratory of Cellular Metabolism, Debrecen, H-4032, Hungary Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Export Date: 6 October 2021 Correspondence Address: Bai, P.; Department of Medical Chemistry, Hungary; email: baip@med.unideb.hu Correspondence Address: Bokor, É.; Department of Organic Chemistry, P.O. Box 400, Hungary; email: bokor.eva@science.unideb.hu Funding details: GINOP‐2.3.2‐15‐2016‐00006, GINOP‐2.3.2‐15‐2016‐00008, GINOP‐2.3.3‐15‐2016‐00004 Funding details: FK125067, K123975 Funding details: European Commission, EC Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Regional Development Fund, ERDF, GINOP-2.3.2-15-2016-00008, GINOP-2.3.3-15-2016-00004 Funding details: Debreceni Egyetem, DE, TKP2020‐IKA‐04 Funding text 1: Our work was supported by the National Research, Development, and Innovation Office of Hungary (grants K123975 and FK125067), by the EU co-financed by the European Regional Development Fund under projects GINOP-2.3.2-15-2016-00006, GINOP-2.3.2-15-2016-00008, and GINOP-2.3.3-15-2016-00004, by the Momentum fellowship of the Hungarian Academy of Sciences and the University of Debrecen, and by the Thematic Excellence Program (TKP2020-IKA-04) of the Ministry for Innovation and Technology in Hungary. We are grateful for Mr. L?szl? Finta for the technical assistance, as well as Attila T?th and Eszter Janka (both at the University of Debrecen) for their advice and help with statistical calculations. Funding text 2: Funding: Our work was supported by the National Research, Development, and Innovation Office of Hungary (grants K123975 and FK125067), by the EU co‐financed by the European Regional De‐ velopment Fund under projects GINOP‐2.3.2‐15‐2016‐00006, GINOP‐2.3.2‐15‐2016‐00008, and GINOP‐2.3.3‐15‐2016‐00004, by the Momentum fellowship of the Hungarian Academy of Sciences and the University of Debrecen, and by the Thematic Excellence Program (TKP2020‐IKA‐04) of the Ministry for Innovation and Technology in Hungary. AB - Ruthenium complexes are developed as substitutes for platinum complexes to be used in the chemotherapy of hematological and gynecological malignancies, such as ovarian cancer. We synthesized and screened 14 ruthenium half-sandwich complexes with bidentate monosaccharide ligands in ovarian cancer cell models. Four complexes were cytostatic, but not cytotoxic on A2780 and ID8 cells. The IC50 values were in the low micromolar range (the best being 0.87 µM) and were similar to or lower than those of the clinically available platinum complexes. The active complexes were cytostatic in cell models of glioblastoma, breast cancer, and pancreatic adenocarcinoma, while they were not cytostatic on non-transformed human skin fibroblasts. The bioactive ruthenium complexes showed cooperative binding to yet unidentified cellular target(s), and their activity was dependent on reactive oxygen species production. Large hydrophobic protective groups on the hydroxyl groups of the sugar moiety were needed for biological activity. The cytostatic activity of the ruthenium complexes was dependent on reactive species production. Rucaparib, a PARP inhibitor, potentiated the effects of ruthenium complexes. LA - English DB - MTMT ER -