TY - JOUR AU - Pfliegler, Valter Péter AU - Imre, Alexandra AU - Pócsi, István TI - PCR-fingerprinting of culturable yeasts from commercially obtained beers: a simple and engaging applied microbiological laboratory exercise JF - MICROPUBLICATION BIOLOGY J2 - MICROPUBL BIOL VL - 2025 PY - 2025 IS - 2 SN - 2578-9430 UR - https://m2.mtmt.hu/api/publication/35795618 ID - 35795618 AB - Yeast and fermented products present an opportunity to introduce students to applied microbiology. We designed and implemented a project-oriented laboratory class where yeasts from bottled beverages were isolated and compared using DNA fingerprinting and Sanger-sequencing. We recovered 17 Saccharomyces isolates, and two non-Saccharomyces yeasts. Fingerprinting identified two groups of closely related Saccharomyces isolates in unrelated beer styles, later identified as diastatic and wine yeasts using phylogenomics. Isolates from traditional products thus may not represent the original fermentation. We believe that the interlinked nature of topics and the simple basis can elevate engagement and performance of students during such a class. LA - English DB - MTMT ER - TY - CONF AU - Imre, Alexandra AU - Kovács, Renátó AU - Ibrahim, Al’ Abri AU - Nathan, Crook AU - Pfliegler, Valter Péter TI - Specific high effect mutations in clinical and experimentally evolved Saccharomyces ‘boulardii’ isolates show that genes involved in chemical response might have a role during the adaptation to the human host T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753304 ID - 34753304 LA - English DB - MTMT ER - TY - CONF AU - Pfliegler, Valter Péter AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Bálint, P. Németh AU - Andrea, P. Harmath AU - Katalin, P. Pappné Murvai AU - Rizagul, P. Bazenova AU - Malika, P. Kultazina AU - Marium, P. Farooq AU - Reda, M. Lemsali AU - Antunovics, Zsuzsa AU - Kovács, Renátó AU - Pócsi, István TI - From macro- to microevolution in the yeast S. cerevisiae T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753321 ID - 34753321 LA - English DB - MTMT ER - TY - JOUR AU - Pappné Murvai, Katalin AU - Rácz, Hanna Viktória AU - Horváth, Enikő AU - Németh, Bálint AU - Imre, Alexandra AU - Pereira, Kadmiel Naliel Oliveira AU - Antunovics, Zsuzsa AU - Peles, Ferenc Árpád AU - Sipos, Péter AU - Béri, Béla AU - Pusztahelyi, Tünde AU - Pócsi, István AU - Pfliegler, Valter Péter TI - The bacterial and yeast microbiota in livestock forages in Hungary JF - BMC MICROBIOLOGY J2 - BMC MICROBIOL VL - 24 PY - 2024 IS - 1 PG - 11 SN - 1471-2180 DO - 10.1186/s12866-024-03499-8 UR - https://m2.mtmt.hu/api/publication/35272544 ID - 35272544 AB - Background Along bacteria, yeasts are common in forages and forage fermentations as spoilage microbes or as additives, yet few studies exist with species-level data on these fungi's occurrence in feedstuff. Active dry yeast and other yeast-based products are also common feed additives in animal husbandry. Here, we aimed to characterize both fermented and non-fermented milking cow feedstuff samples from Hungary to assess their microbial diversity in the first such study from Central Europe. Results We applied long-read bacterial metabarcoding to 10 fermented and 25 non-fermented types of samples to assess bacterial communities and their characteristics, surveyed culturable mold and yeast abundance, and identified culturable yeast species. Fermented forages showed the abundance of Aerococcaceae, Bacillaceae, Brucellaceae, Lactobacillaceae, Staphylococcaceae, and Thermoactinomycetaceae, non-fermented ones had Cyanothecaceae, Enterobacteriaceae, Erwiniaceae, Gomontiellaceae, Oxalobacteraceae, Rhodobiaceae, Rickettsiaceae, and Staphylococcaceae. Abundances of bacterial families showed mostly weak correlation with yeast CFU numbers, only Microcoleaceae (positive) and Enterococcaceae and Alcaligenaceae (negative correlation) showed moderate correlation. We identified 14 yeast species, most commonly Diutina rugosa, Pichia fermentans, P. kudriavzevii, and Wickerhahomyces anomalus. We recorded S. cerevisiae isolates only from animal feed mixes with added active dry yeast, while the species was completely absent from fermented forages. The S. cerevisiae isolates showed high genetic uniformity. Conclusion Our results show that both fermented and non-fermented forages harbor diverse bacterial microbiota, with higher alpha diversity in the latter. The bacterial microbiome had an overall weak correlation with yeast abundance, but yeasts were present in the majority of the samples, including four new records for forages as a habitat for yeasts. Yeasts in forages mostly represented common species including opportunistic pathogens, along with a single strain of Saccharomyces used as a feed mix additive. LA - English DB - MTMT ER - TY - CONF AU - Pfliegler, Valter Péter AU - Németh, Bálint AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Harmath, Andrea AU - Pappné Murvai, Katalin AU - Antunovics, Zsuzsa AU - Pócsi, István AU - Kovács, Renátó TI - 200 years of research, hundreds of publications, 4500 genomes: a global view on what is known and unknown about the phylogenomic diversity of Saccharomyces. T2 - 16th International Congress on Yeasts Congress Abstracts PY - 2024 SP - 167 UR - https://m2.mtmt.hu/api/publication/35441958 ID - 35441958 LA - English DB - MTMT ER - TY - CONF AU - Németh, Bálint AU - Harmath, Andrea AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Pócsi, István AU - Kovács, Renátó AU - Pfliegler, Valter Péter TI - A Compendium of Saccharomyces yeasts with sequenced genomes. T2 - 16th International Congress on Yeasts Congress Abstracts PY - 2024 SP - 143 UR - https://m2.mtmt.hu/api/publication/35441973 ID - 35441973 LA - English DB - MTMT ER - TY - CONF AU - Harmath, Andrea AU - Németh, Bálint AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Majoros, László AU - Pfliegler, Valter Péter AU - Kovács, Renátó TI - Most of the phylogenomic diversity of Saccharomyces cerevisiae is recapitulated among human isolates from a single hospital T2 - 16th International Congress on Yeasts Congress Abstracts PY - 2024 SP - 78 UR - https://m2.mtmt.hu/api/publication/35442012 ID - 35442012 LA - English DB - MTMT ER - TY - CONF AU - Németh, Bálint AU - Harmath, Andrea AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Pappné Murvai, Katalin AU - Kovács, Renátó AU - Pócsi, István AU - Pfliegler, Valter Péter TI - A saccharomyces élesztők genomi kompendiuma: a vad izolátumoktól az ipari törzseken át a patogénekig T2 - Biotechnológia a Debreceni Egyetemen 2024 Szimpózium : Absztraktfüzet PB - Debreceni Egyetem C1 - Debrecen SN - 9789634906612 PY - 2024 SP - 47 UR - https://m2.mtmt.hu/api/publication/35727227 ID - 35727227 LA - Hungarian DB - MTMT ER - TY - CONF AU - Pfliegler, Valter Péter AU - Kállai, Zoltán AU - Aizhan, Toxeitova AU - Imre, Alexandra AU - Németh, Bálint AU - Harmath, Andrea AU - Pócsi, István TI - Mead yeasts: a student research project at the University of Debrecen T2 - Magyar Mikrobiológiai Társaság 2024. évi Nagygyűlése/ Hungarian Society for Microbiology General Meeting 2024. - Absztraktok/ Abstracts PB - Magyar Mikrobiológiai Társaság (MMT) C1 - Siófok PY - 2024 SP - 77 EP - 78 PG - 2 UR - https://m2.mtmt.hu/api/publication/35744206 ID - 35744206 LA - English DB - MTMT ER - TY - CONF AU - Pfliegler, Valter Péter AU - Rácz, Hanna Viktória AU - Imre, Alexandra ED - László, Buday ED - Gábor, Juhász ED - Beáta, Lontay ED - Mihály, József ED - Sinka, Rita ED - László, Virág ED - Attila, Varga ED - Gergely, Szakáts TI - The macro- and microevolution of the yeast S. cerevisiae in the host with experimental evolution, genomics and metagenomics. T2 - Hungarian Molecular Life Sciences 2023: Book of Abstracts PB - Diamond Congress Kft. CY - Budapest SN - 9786155270772 PY - 2023 UR - https://m2.mtmt.hu/api/publication/34161850 ID - 34161850 LA - English DB - MTMT ER -