@article{MTMT:35795618,
	title = {PCR-fingerprinting of culturable yeasts from commercially obtained beers: a simple and engaging applied microbiological laboratory exercise},
	url = {https://m2.mtmt.hu/api/publication/35795618},
	author = {Pfliegler, Valter Péter and Imre, Alexandra and Pócsi, István},
	journal-iso = {MICROPUBL BIOL},
	journal = {MICROPUBLICATION BIOLOGY},
	volume = {2025},
	unique-id = {35795618},
	abstract = {Yeast and fermented products present an opportunity to introduce students to applied microbiology. We designed and implemented a project-oriented laboratory class where yeasts from bottled beverages were isolated and compared using DNA fingerprinting and Sanger-sequencing. We recovered 17 Saccharomyces isolates, and two non-Saccharomyces yeasts. Fingerprinting identified two groups of closely related Saccharomyces isolates in unrelated beer styles, later identified as diastatic and wine yeasts using phylogenomics. Isolates from traditional products thus may not represent the original fermentation. We believe that the interlinked nature of topics and the simple basis can elevate engagement and performance of students during such a class.},
	year = {2025},
	eissn = {2578-9430},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:34753304,
	title = {Specific high effect mutations in clinical and experimentally evolved Saccharomyces ‘boulardii’ isolates show that genes involved in chemical response might have a role during the adaptation to the human host},
	url = {https://m2.mtmt.hu/api/publication/34753304},
	author = {Imre, Alexandra and Kovács, Renátó and Ibrahim, Al’ Abri and Nathan, Crook and Pfliegler, Valter Péter},
	booktitle = {The Allied Genetics Conference 2024 Abstract Book},
	unique-id = {34753304},
	year = {2024},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:34753321,
	title = {From macro- to microevolution in the yeast S. cerevisiae},
	url = {https://m2.mtmt.hu/api/publication/34753321},
	author = {Pfliegler, Valter Péter and Imre, Alexandra and Rácz, Hanna Viktória and Bálint, P. Németh and Andrea, P. Harmath and Katalin, P. Pappné Murvai and Rizagul, P. Bazenova and Malika, P. Kultazina and Marium, P. Farooq and Reda, M. Lemsali and Antunovics, Zsuzsa and Kovács, Renátó and Pócsi, István},
	booktitle = {The Allied Genetics Conference 2024 Abstract Book},
	unique-id = {34753321},
	year = {2024},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@article{MTMT:35272544,
	title = {The bacterial and yeast microbiota in livestock forages in Hungary},
	url = {https://m2.mtmt.hu/api/publication/35272544},
	author = {Pappné Murvai, Katalin and Rácz, Hanna Viktória and Horváth, Enikő and Németh, Bálint and Imre, Alexandra and Pereira, Kadmiel Naliel Oliveira and Antunovics, Zsuzsa and Peles, Ferenc Árpád and Sipos, Péter and Béri, Béla and Pusztahelyi, Tünde and Pócsi, István and Pfliegler, Valter Péter},
	doi = {10.1186/s12866-024-03499-8},
	journal-iso = {BMC MICROBIOL},
	journal = {BMC MICROBIOLOGY},
	volume = {24},
	unique-id = {35272544},
	abstract = {Background  Along bacteria, yeasts are common in forages and forage fermentations as spoilage microbes or as additives, yet few studies exist with species-level data on these fungi's occurrence in feedstuff. Active dry yeast and other yeast-based products are also common feed additives in animal husbandry. Here, we aimed to characterize both fermented and non-fermented milking cow feedstuff samples from Hungary to assess their microbial diversity in the first such study from Central Europe.    Results  We applied long-read bacterial metabarcoding to 10 fermented and 25 non-fermented types of samples to assess bacterial communities and their characteristics, surveyed culturable mold and yeast abundance, and identified culturable yeast species. Fermented forages showed the abundance of Aerococcaceae, Bacillaceae, Brucellaceae, Lactobacillaceae, Staphylococcaceae, and Thermoactinomycetaceae, non-fermented ones had Cyanothecaceae, Enterobacteriaceae, Erwiniaceae, Gomontiellaceae, Oxalobacteraceae, Rhodobiaceae, Rickettsiaceae, and Staphylococcaceae. Abundances of bacterial families showed mostly weak correlation with yeast CFU numbers, only Microcoleaceae (positive) and Enterococcaceae and Alcaligenaceae (negative correlation) showed moderate correlation. We identified 14 yeast species, most commonly Diutina rugosa, Pichia fermentans, P. kudriavzevii, and Wickerhahomyces anomalus. We recorded S. cerevisiae isolates only from animal feed mixes with added active dry yeast, while the species was completely absent from fermented forages. The S. cerevisiae isolates showed high genetic uniformity.    Conclusion  Our results show that both fermented and non-fermented forages harbor diverse bacterial microbiota, with higher alpha diversity in the latter. The bacterial microbiome had an overall weak correlation with yeast abundance, but yeasts were present in the majority of the samples, including four new records for forages as a habitat for yeasts. Yeasts in forages mostly represented common species including opportunistic pathogens, along with a single strain of Saccharomyces used as a feed mix additive.},
	year = {2024},
	eissn = {1471-2180},
	orcid-numbers = {Sipos, Péter/0000-0002-8168-850X; Pusztahelyi, Tünde/0000-0002-5495-6273; Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:35441958,
	title = {200 years of research, hundreds of publications, 4500 genomes: a global view on what is known and unknown about the phylogenomic diversity of Saccharomyces.},
	url = {https://m2.mtmt.hu/api/publication/35441958},
	author = {Pfliegler, Valter Péter and Németh, Bálint and Imre, Alexandra and Rácz, Hanna Viktória and Harmath, Andrea and Pappné Murvai, Katalin and Antunovics, Zsuzsa and Pócsi, István and Kovács, Renátó},
	booktitle = {16th International Congress on Yeasts Congress Abstracts},
	unique-id = {35441958},
	year = {2024},
	pages = {167},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:35441973,
	title = {A Compendium of Saccharomyces yeasts with sequenced genomes.},
	url = {https://m2.mtmt.hu/api/publication/35441973},
	author = {Németh, Bálint and Harmath, Andrea and Imre, Alexandra and Rácz, Hanna Viktória and Pócsi, István and Kovács, Renátó and Pfliegler, Valter Péter},
	booktitle = {16th International Congress on Yeasts Congress Abstracts},
	unique-id = {35441973},
	year = {2024},
	pages = {143},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:35442012,
	title = {Most of the phylogenomic diversity of Saccharomyces cerevisiae is recapitulated among human isolates from a single hospital},
	url = {https://m2.mtmt.hu/api/publication/35442012},
	author = {Harmath, Andrea and Németh, Bálint and Imre, Alexandra and Rácz, Hanna Viktória and Majoros, László and Pfliegler, Valter Péter and Kovács, Renátó},
	booktitle = {16th International Congress on Yeasts Congress Abstracts},
	unique-id = {35442012},
	year = {2024},
	pages = {78},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:35727227,
	title = {A saccharomyces élesztők genomi kompendiuma: a vad izolátumoktól az ipari törzseken át a patogénekig},
	url = {https://m2.mtmt.hu/api/publication/35727227},
	author = {Németh, Bálint and Harmath, Andrea and Imre, Alexandra and Rácz, Hanna Viktória and Pappné Murvai, Katalin and Kovács, Renátó and Pócsi, István and Pfliegler, Valter Péter},
	booktitle = {Biotechnológia a Debreceni Egyetemen 2024 Szimpózium : Absztraktfüzet},
	unique-id = {35727227},
	year = {2024},
	pages = {47},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:35744206,
	title = {Mead yeasts: a student research project at the University of Debrecen},
	url = {https://m2.mtmt.hu/api/publication/35744206},
	author = {Pfliegler, Valter Péter and Kállai, Zoltán and Aizhan, Toxeitova and Imre, Alexandra and Németh, Bálint and Harmath, Andrea and Pócsi, István},
	booktitle = {Magyar Mikrobiológiai Társaság 2024. évi Nagygyűlése/ Hungarian Society for Microbiology General Meeting 2024. - Absztraktok/ Abstracts},
	unique-id = {35744206},
	year = {2024},
	pages = {77-78},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}

@CONFERENCE{MTMT:34161850,
	title = {The macro- and microevolution of the yeast S. cerevisiae in the host with experimental evolution, genomics and metagenomics.},
	url = {https://m2.mtmt.hu/api/publication/34161850},
	author = {Pfliegler, Valter Péter and Rácz, Hanna Viktória and Imre, Alexandra},
	booktitle = {Hungarian Molecular Life Sciences 2023: Book of Abstracts},
	unique-id = {34161850},
	year = {2023},
	orcid-numbers = {Pfliegler, Valter Péter/0000-0001-6723-4416}
}