TY - JOUR AU - Csató-Kovács, Erika AU - Salamon, Pál Attila AU - Fikó-Lászlo, Szilvia AU - Kovács, Krisztina AU - Koka, Alice AU - András-Korodi, Mónika AU - Antal, Emőke AU - Brumă, Emília AU - Tőrsők, Brigitta AU - Gudor, Szilárd AU - Miklóssy, Ildikó AU - Orbán, Kálmán Csongor AU - Albert, Csilla AU - Bálint, Emese Éva AU - Albert, Beáta TI - Development of a Mammalian Cell Line for Stable Production of Anti-PD-1 JF - ANTIBODIES J2 - ANTIBODIES VL - 13 PY - 2024 IS - 4 PG - 13 SN - 2073-4468 DO - 10.3390/antib13040082 UR - https://m2.mtmt.hu/api/publication/35434014 ID - 35434014 AB - Background/Objectives: Immune checkpoint blockade, particularly targeting the programmed cell death 1 (PD-1) receptor, is a promising strategy in cancer immunotherapy. The interaction between PD-1 and its ligands, PD-L1 and PD-L2, is crucial in immune evasion by tumors. Blocking this interaction with monoclonal antibodies like Nivolumab can restore anti-tumor immunity. This study aims to develop a stable expression system for Nivolumab-based anti-PD-1 in the Chinese Hamster Ovary (CHO) DG44 cell line using two different expression vector systems with various signal sequences. Methods: The heavy chain (HC) and light chain (LC) of Nivolumab were cloned into two expression vectors, pOptiVEC and pcDNA3.3. Each vector was engineered with two distinct signal sequences, resulting in the creation of eight recombinant plasmids. These plasmids were co-transfected into CHO DG44 cells in different combinations, allowing for the assessment of stable antibody production. Results: Both pOptiVEC and pcDNA3.3 vectors were successful in stably integrating and expressing the Nivolumab-based anti-PD-1 antibody in CHO DG44 cells. This study found that the choice of signal sequence significantly influenced the quantity of antibodies produced. The optimization of production conditions further enhanced antibody yield, indicating the potential for large-scale production. Conclusions: This study demonstrates that both pOptiVEC and pcDNA3.3 expression systems are effective for the stable production of Nivolumab-based anti-PD-1 in CHO DG44 cells. Signal sequences play a critical role in determining the expression levels, and optimizing production conditions can further increase antibody yield, supporting future applications in cancer immunotherapy. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, Hunor Pál AU - Dávid, Hunor AU - Czont, Attila AU - Miklóssy, Ildikó AU - Orbán, Kálmán-Csongor AU - Tar, Gyöngyi AU - Fodor, Abony AU - Kovács, Zita AU - Albert, Beáta AU - Salamon, Pál Attila TI - Enhanced Gait Recovery in Chronic Post-COVID-19 Stroke: The Role of Combined Physical Rehabilitation JF - REPORTS J2 - REPORTS-BASEL VL - 6 PY - 2023 IS - 4 PG - 18 SN - 2571-841X DO - 10.3390/reports6040051 UR - https://m2.mtmt.hu/api/publication/34238005 ID - 34238005 AB - Background: Rehabilitation programs applied in cases of COVID-19-related stroke should counteract not only the effects of the stroke but also the effects of long-term COVID-19. As the molecular processes underlying these cases are still not fully understood, and evidence-based clinical outcomes are scarcely documented, there is a valid need to gather information and develop rehabilitation strategies for these patients. The risks, already clarified in the case of stroke, need to be assessed taking into account the coincidence of the two diseases. Endothelial injuries and emboli that develop after the hypercoagulable state of COVID-19 may take longer to heal, and complications may occur during exercise. This case study attempts to determine what the rehabilitation of a COVID-19-related stroke patient should include. The participant was a 64-year-old male with ischemic right middle cerebral artery stroke, left-side hemiplegia, and middle cerebral artery stenosis, and the CT showed a well-defined area of hypoattenuation in the basal ganglia territory involving the right lentiform nucleus, the anterior and posterior limbs of the internal capsule, and the dorsal part of the external capsule. His NIHSS score was 14, and he registered 15 points on the Barthel index. The patient had a COVID-19 infection two weeks before the stroke event. Methods: Conventional physical therapy was combined with adaptive ballistic strength training, a high-intensity interval training regimen, and manual treatment for myofascial release throughout the chronic recovery phase. Our primary goals were gait rehabilitation, muscle strengthening, weakness management, as well as spasticity reduction, while three different rehabilitation approaches were adopted in a single rehabilitation program to improve the outcome and long-term functional recovery of the patient. Results: The patient progressed in almost every aspect of the assessment criteria. This combined approach’s main success was improved gait speed, gait quality, and improved cardiovascular fitness. Take-away message: In the case of a stroke caused by COVID-19, where the endothelium cells are compromised, HIIT may be questionable due to the poor vascular condition. Based on our results, the low-volume HIIT approach proved appropriate and effective. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zita AU - Bálint, Emese-Éva AU - Lászlo, Szilvia AU - Albert, Beáta AU - Gyöngyi, Tar AU - Attila, András AU - Salamon, Pál Attila TI - THE EFFECT OF SARS-COV-2 VIRUS ON THE ACTIVITY OF THE PANOPTOSIS PATHWAY IN UPPER RESPIRATORY EPITHELIAL CELLS: CORRELATIONS BETWEEN EXPRESSION PATTERNS, AGE, GENDER, AND COMORBIDITIES JF - ACTA MARISIENSIS: SERIA MEDICA J2 - ACTA MARISIEN VL - 69 PY - 2023 IS - Suppl. 5 SP - 35 EP - 35 PG - 1 SN - 2668-7755 UR - https://m2.mtmt.hu/api/publication/34109004 ID - 34109004 LA - English DB - MTMT ER - TY - JOUR AU - Csányi, Mária Csilla AU - Salamon, Pál Attila AU - Feller, Tímea AU - Bozó, Tamás AU - Hársfalvi, Jolán AU - Kellermayer, Miklós TI - Structural hierarchy of mechanical extensibility in human von Willebrand factor multimers JF - PROTEIN SCIENCE J2 - PROTEIN SCI VL - 32 PY - 2023 IS - 1 PG - 14 SN - 0961-8368 DO - 10.1002/pro.4535 UR - https://m2.mtmt.hu/api/publication/33365403 ID - 33365403 LA - English DB - MTMT ER - TY - CONF AU - Csányi, Mária Csilla AU - Salamon, Pál Attila AU - Feller, Tímea AU - Bozó, Tamás AU - Hársfalvi, Jolán AU - Kellermayer, Miklós S.Z. ED - Kilár, Ferenc ED - Nagy, Laura TI - Force dependent multimer glycoprotein elongation T2 - 20th INTERNATIONAL SYMPOSIUM AND SUMMER SCHOOL ON BIOANALYSIS Book of Abstracts C1 - Pécs PY - 2022 SP - 64 EP - 64 PG - 1 UR - https://m2.mtmt.hu/api/publication/33550801 ID - 33550801 N1 - Poszter prezentáció LA - English DB - MTMT ER - TY - JOUR AU - Salamon, Pál Attila AU - Orbán, Kálmán Csongor AU - Molnar-Nagy, Katalin AU - Kovács, Zita AU - Vancsa, Klara AU - Bálint, Emese-Éva AU - Miklossy, Ildiko AU - Albert, Beáta AU - Tar, Gyongyi AU - Lanyi, Szabolcs TI - Study of native SMAC protein production in the pUbiq expression system: Molecular cloning, biosynthesis and molecular modelling JF - ELECTRONIC JOURNAL OF BIOTECHNOLOGY J2 - ELECTRON J BIOTECHN VL - 56 PY - 2022 SP - 39 EP - 46 PG - 8 SN - 0717-3458 DO - 10.1016/j.ejbt.2022.01.0020717-3458 UR - https://m2.mtmt.hu/api/publication/33345866 ID - 33345866 AB - Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps. Nonetheless these tags might alter enzyme specificity and activity, therefore in functional assays it is recommended to use authentic or native proteins. Several ubiquitin fusion systems have been developed for E. coli-based recombinant protein expression that provide high levels of expression, with simple purification, and allow the production of various proteins with authentic N-terminus for subsequent applications. Results: In the present research, we describe an ubiquitin fused bacterial biosynthetic system (pUbiq) for the production of the native Second mitochondria-derived activator of caspases (SMAC) recombinant protein. Using this system, the recombinant protein is expressed with an ubiquitin-decahistidine fusion partner, then purified from the cell-forming proteins by affinity chromatography. The fusion partner is then removed by proteolytic digestion, resulting the native structure of the recombinant protein without unnecessary amino acid residues. Following proteolysis, another affinity chromatography method is used to separate the native protein from the fusion partner and the proteolytic enzyme. The folding of the protein of interest was verified by a pull-down assay. Conclusions: Based on our results, the presented pUbiq system was successfully applied in the production of native SMAC recombinant protein, where the affinity tag required for purification was completely removed. Our study suggests that the ubiquitin-fusion technology will be useful for enhancing expression and purification of native and authentic proteins for structural and functional studies as well as for therapeutic uses. How to cite: Salamona P, Orbana CK, Molnar-Nagy K, et al. Exon based amplified polymorphism (EBAP): A novel and universal molecular marker for plants. Electron J Biotechnol 2022;56. https://doi.org/10.10 16/j.ejbt.2022.01.002. (c) 2022 Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). LA - English DB - MTMT ER - TY - JOUR AU - Bardocz-Veres, Zsuzsanna AU - Székely, Melinda AU - Salamon, Pál Attila AU - Bala, Előd AU - Bereczki, Előd AU - Kerekes-Máthé, Bernadette TI - Quantitative and Qualitative Assessment of Fluorescence in Aesthetic Direct Restorations JF - MATERIALS J2 - MATERIALS VL - 15 PY - 2022 IS - 13 SP - 4619 SN - 1996-1944 DO - 10.3390/ma15134619 UR - https://m2.mtmt.hu/api/publication/32915059 ID - 32915059 LA - English DB - MTMT ER - TY - JOUR AU - Salamon, Pál Attila AU - Orbán, Kálmán Csongor AU - Ildikó, MIKLÓSSY AU - Albert, Beáta AU - Szabolcs, LÁNYI TI - COMPARATIVE STUDY ON CONVENTIONAL AND AUTOINDUCTION FERMENTATION JF - UPB SCIENTIFIC BULLETIN, SERIES B: CHEMISTRY AND MATERIALS SCIENCE J2 - UPB SCI BULL SER B VL - 83 PY - 2021 IS - 2 SP - 77 SN - 1454-2331 UR - https://m2.mtmt.hu/api/publication/32923364 ID - 32923364 LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Katalin AU - Kovács, Zita AU - Miklóssy, Ildikó AU - Salamon, Pál Attila AU - Orbán, Kálmán Csongor AU - Albert, Beáta AU - Lányi, Szabolcs TI - Detergent aided refolding and purification of recombinant XIAP from inclusion bodies JF - STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA J2 - STUD UNIV BABES-BOLYAI CHEM VL - 66 PY - 2021 IS - 4 SP - 355 EP - 368 PG - 14 SN - 1224-7154 DO - 10.24193/subbchem.2021.4.26 UR - https://m2.mtmt.hu/api/publication/32547005 ID - 32547005 LA - English DB - MTMT ER - TY - JOUR AU - János, SZÖVÉRFI AU - Orbán, Kálmán Csongor AU - Albert, Beáta AU - Katalin, NAGY AU - Salamon, Pál Attila AU - Szabolcs, LÁNYI TI - IN VITRO STUDY OF THE CCMV CAPSID PROTEIN: CLONING, EXPRESSION, AND PURIFICATION JF - UPB SCIENTIFIC BULLETIN, SERIES B: CHEMISTRY AND MATERIALS SCIENCE J2 - UPB SCI BULL SER B VL - 83 PY - 2021 IS - 1 SP - 135 EP - 142 PG - 8 SN - 1454-2331 UR - https://m2.mtmt.hu/api/publication/32069722 ID - 32069722 AB - In our current work we studied the expression of the Cowpea chlorotic mottle virus (CCMV) capsid protein in E. coli host cell. The CCMV proteins DNA was cloned into a vector containing a His-tag and a ubiquitin fusion partner. The gene was transformed in E. coli BL21(DE3) Rosetta cells and expressed. The expression process was optimized and shake flask expression was conducted on 37 degrees C and 0.1 mM IPTG concentration. Cloning and expressing of CCMV capsid protein was successful, confirmed by sequencing results. The protein was purified with Ni-affinity chromatography. LA - English DB - MTMT ER -