TY - JOUR AU - Jójárt, Boldizsár AU - Resál, Tamás AU - Kata, Diána AU - Molnár, Tünde AU - Bacsur, Péter AU - Szabó, Viktória AU - Varga, Árpád AU - Szántó, Kata Judit AU - Pallagi, Petra AU - Földesi, Imre AU - Molnár, Tamás AU - Maléth, József AU - Farkas, Klaudia TI - P421 The faecal biomarker LDN-051 is a novel tool for monitoring disease activity and therapeutic response in Inflammatory Bowel Diseases JF - JOURNAL OF CROHNS & COLITIS J2 - J CROHNS COLITIS VL - 18 PY - 2024 IS - Supplement_1 SP - i869 EP - i869 PG - 1 SN - 1873-9946 DO - 10.1093/ecco-jcc/jjad212.0551 UR - https://m2.mtmt.hu/api/publication/34538603 ID - 34538603 LA - English DB - MTMT ER - TY - JOUR AU - Jójárt, Boldizsár AU - Resál, Tamás AU - Kata, Diána AU - Molnár, Tünde AU - Bacsur, Péter AU - Szabó, Viktória AU - Varga, Árpád AU - Szántó, Kata Judit AU - Pallagi, Petra AU - Földesi, Imre AU - Molnár, Tamás AU - Maléth, József AU - Farkas, Klaudia TI - Plasminogen activator inhibitor 1 is a novel faecal biomarker for monitoring disease activity and therapeutic response in inflammatory bowel diseases JF - JOURNAL OF CROHNS & COLITIS J2 - J CROHNS COLITIS VL - 18 PY - 2024 IS - 3 SP - 392 EP - 405 PG - 14 SN - 1873-9946 DO - 10.1093/ecco-jcc/jjad160 UR - https://m2.mtmt.hu/api/publication/34167015 ID - 34167015 N1 - Ladon Therapeutics Ltd, Szeged, Hungary Department of Medicine, University of Szeged, Szeged, Hungary ELKH-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, University of Szeged, Szeged, Hungary HCEMM-USZ Molecular Gastroenterology Research Group, University of Szeged, Szeged, Hungary Faculty of Medicine, Institute of Laboratory Medicine, University of Szeged, Szeged, Hungary Export Date: 8 April 2024 Correspondence Address: Maléth, J.; HAS-USZ Momentum Epithelial Cell Signalling and Secretion Research Group, Hungary; email: maleth.jozsef@med.uszeged.hu Correspondence Address: Farkas, K.; HAS-USZ Momentum Epithelial Cell Signalling and Secretion Research Group, Hungary Chemicals/CAS: adalimumab, 331731-18-1, 1446410-95-2; azathioprine, 446-86-6, 55774-33-9; budesonide, 51333-22-3, 51372-29-3; cyclosporine, 59865-13-3, 63798-73-2, 79217-60-0; infliximab, 170277-31-3; mesalazine, 89-57-6; plasminogen activator inhibitor 1, 140208-23-7; tofacitinib, 477600-75-2, 540737-29-9; ustekinumab, 815610-63-0, 949907-93-1; vedolizumab, 943609-66-3; Biomarkers; Plasminogen Activator Inhibitor 1 Tradenames: 201170, Greiner; ab269373, Abcam; ChemiDoc MP, Biorad; GraphPad 9.5.0, Graphpad; iScript cDNA Synthesis Kit, Biorad; MedCalc Software 20.211, MedCalc; Multiskan FC, Thermo; NucleoSpin RNA Plus Kit, Macherey Manufacturers: Abcam; Biorad; Carl Zeiss; Graphpad; Greiner; Macherey; MedCalc; Thermo AB - Crohn's disease and ulcerative colitis require lifelong treatment and patient monitoring. Current biomarkers have several limitations, therefore, there is an unmet need to identify novel biomarkers in inflammatory bowel disease (IBD). Previously, the role of plasminogen activator inhibitor 1 (PAI-1) was established in the pathogenesis of IBD and suggested as a potential biomarker. Therefore, we aimed to comprehensively analyze the selectivity of PAI-1 in IBD, its correlation with the disease activity, and its potential to predict therapeutic response.Blood, colon biopsy, organoid cultures (OC), and faecal samples were used from active and inactive IBD patients and control subjects. Serpin E1 gene expressions and PAI-1 protein levels and localization in serum, biopsy, and fecal samples were evaluated by qRT-PCR, ELISA, and immunostaining, respectively.The study population comprised 132 IBD patients (56 CD and 76 UC) and 40 non-IBD patients. We demonstrated that the serum, mucosal, and faecal PAI-1 concentration is elevated in IBD patients, showing clinical and endoscopic activity. In responders (decrease of eMayo≥3 in UC; or SES-CD>50% in CD), the initial PAI-1 level decreased significantly upon successful therapy. OCs derived from active IBD patients produced higher concentrations of PAI-1 than the controls, suggesting that epithelial cells could be a source of PAI-1. Moreover, faecal PAI-1 selectively increases in active IBD but not other organic gastrointestinal diseases.The serum, mucosal, and faecal PAI-1 concentration correlates with the disease activity and therapeutic response in IBD, suggesting that PAI-1 could be utilized as a novel non-invasive, disease-specific faecal biomarker in the patient follow-up. LA - English DB - MTMT ER - TY - GEN AU - Szabó, Viktória AU - Csákány-Papp, Noémi AU - Görög, Marietta AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Tél, Bálint AU - Jójárt, Boldizsár AU - Crul, Tim AU - Dudás, Krisztina Márta AU - Bagyánszki, Mária AU - Bódi, Nikolett AU - Ayaydin, Ferhan AU - Jee, Shyam AU - Tiszlavicz, László AU - Stauderman, Kenneth AU - Hebbar, Sudarshan AU - Pallagi, Petra AU - Maléth, József TI - Orai1 calcium channel inhibition prevents progression of chronic pancreatitis PY - 2023 UR - https://m2.mtmt.hu/api/publication/34687647 ID - 34687647 LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Viktória AU - Csákány-Papp, Noémi AU - Görög, Marietta AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, AK AU - Tél, Bálint AU - Jójárt, Boldizsár AU - Crul, Tim AU - Dudás, Krisztina Márta AU - Bagyánszki, M AU - Bódi, N AU - Ayaydin, F AU - Jee, S AU - Tiszlavicz, L AU - Stauderman, K AU - Hebbar, S AU - Pallagi, Petra AU - Maléth, József TI - ORAI1 CALCIUM CHANNEL INHIBITION PREVENTS PROGRESSION OF RECURRENT ACUTE AND CHRONIC PANCREATITIS JF - UNITED EUROPEAN GASTROENTEROLOGY JOURNAL J2 - UEG JOURNAL VL - 11 PY - 2023 IS - Suppl 8 SP - 66 EP - 66 PG - 1 SN - 2050-6406 UR - https://m2.mtmt.hu/api/publication/34687430 ID - 34687430 LA - English DB - MTMT ER - TY - GEN AU - Szabó, Viktória AU - Csákány-Papp, Noémi AU - Görög, Marietta AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Tél, Bálint AU - Jójárt, Boldizsár AU - Crul, Tim AU - Dudás, Krisztina Márta AU - Bagyánszki, Mária AU - Bódi, Nikolett AU - Ayaydin, Ferhan AU - Shyam, Jee AU - Tiszlavicz, László AU - Stauderman, Kenneth AU - Hebbar, Sudarshan AU - Pallagi, Petra AU - Maléth, József TI - The inhibition of Orai1 calcium channel reduces the progression of chronic pancreatitis PY - 2023 SP - 1 UR - https://m2.mtmt.hu/api/publication/34672053 ID - 34672053 LA - English DB - MTMT ER - TY - JOUR AU - Jójárt, Boldizsár AU - Resál, Tamás AU - Kata, Diána AU - Molnár, Tünde AU - Bacsur, Péter AU - Szabó, Viktória AU - Varga, Árpád AU - Szántó, Kata Judit AU - Földesi, Imre AU - Molnár, Tamás AU - Maléth, József AU - Farkas, Klaudia TI - LDN-051 a novel fecal biomarker for monitoring disease activity and therapeutic response in inflammatory bowel diseases – results of a phase 2 clinical trial JF - UNITED EUROPEAN GASTROENTEROLOGY JOURNAL J2 - UEG JOURNAL VL - 11 PY - 2023 IS - S8 SP - 291 EP - 292 PG - 2 SN - 2050-6406 UR - https://m2.mtmt.hu/api/publication/34593890 ID - 34593890 LA - English DB - MTMT ER - TY - JOUR AU - Varga, Árpád AU - Madácsy, Tamara AU - Görög, Marietta AU - Kiss, Aletta Kata AU - Susánszki, Petra AU - Szabó, Viktória AU - Jójárt, Boldizsár AU - Dudás, Krisztina Márta AU - Farkas, Gyula Jr. AU - Szederkényi, Edit AU - Lázár, György ifj AU - Attila, Farkas AU - Ferhan, Ayaydin AU - Pallagi, Petra AU - Maléth, József TI - Human pancreatic ductal organoids with controlled polarity provide a novel ex vivo tool to study epithelial cell physiology JF - CELLULAR AND MOLECULAR LIFE SCIENCES J2 - CELL MOL LIFE SCI VL - 80 PY - 2023 IS - 7 PG - 17 SN - 1420-682X DO - 10.1007/s00018-023-04836-2 UR - https://m2.mtmt.hu/api/publication/34024396 ID - 34024396 N1 - Department of Medicine, University of Szeged, Szeged, Hungary ELRN-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, Department of Medicine, University of Szeged, Szeged, 6720, Hungary HCEMM-USZ Molecular Gastroenterology Research Group, University of Szeged, Szeged, Hungary Department of Surgery, University of Szeged, Szeged, Hungary HCEMM-USZ Functional Cell Biology and Immunology Advanced Core Facility, University of Szeged, Szeged, Hungary Export Date: 18 July 2023 CODEN: CMLSF Correspondence Address: Maléth, J.; Department of Medicine, Hungary; email: jozsefmaleth1@gmail.com Funding details: 739593 Funding details: Magyar Tudományos Akadémia, MTA, LP2017-18/2017 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, 20391-3/2018/FEKUSTRAT, FK139269, TKP2021-EGA-28, UNKP-21-4-SZTE-116 Funding details: Magyarország Kormánya, NTP-NFTÖ-21-B-0079, NTP-NFTÖ-21-B-0205, NTP-NFTÖ-B-0011 Funding details: Szent-Györgyi Albert Orvostudományi Kar, Szegedi Tudományegyetem Funding text 1: Open access funding provided by University of Szeged. The research was supported by the Momentum Grant of the Hungarian Academy of Sciences (LP2017-18/2017 to JM), by funding from the Hungarian National Research, Development and Innovation Office (FK139269 to JM), by the National Excellence Program (20391-3/2018/FEKUSTRAT and TKP2021-EGA-28 to JM and UNKP-21-4-SZTE-116 to TM). The project has received funding from the EU’s Horizon 2020 Framework Programme research and innovation program under Grant agreement no. 739593. The project was also supported by funding from the Hungarian Government (NTP-NFTÖ-B-0011 to ÁV, NTP-NFTÖ-21-B-0205 to AK and NTP-NFTÖ-21-B-0079 to VSZ). This work was also supported by the Albert Szent-Györgyi Research Grant by the Albert Szent-Györgyi Medical School, University of Szeged to JM. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Viktória AU - Csákány-Papp, Noémi AU - Görög, Marietta AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Tél, Bálint AU - Jójárt, Boldizsár AU - Dudás, Krisztina Márta AU - Bagyánszki, Mária AU - Bódi, Nikolett AU - Ayaydin, F. AU - Shyam, J. AU - Tiszlavicz, László AU - Stauderman, K. AU - Hebbar, S. AU - Pallagi, Petra AU - Maléth, József TI - The inhibition of ORAI1 CA2+ channel prevents the progression of recurrent acute pancreatitis to chronic pancreatitis in mice JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 9 PY - 2023 IS - Suppl. 1 SP - 101 EP - 102 PG - 2 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/34012222 ID - 34012222 LA - English DB - MTMT ER - TY - JOUR AU - Susánszki, Petra AU - Varga, Árpád AU - Görög, Marietta AU - Szabó, Viktória AU - Madácsy, Tamara AU - Dudás, Krisztina Márta AU - Kiss, Aletta Kata AU - Pallagi, Petra AU - Maléth, József TI - CFTR regulatory machinery as a potential novel therapeutic target in pancreatic ductal adenocarcinomas JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 9 PY - 2023 IS - Suppl. 1 SP - 101 EP - 101 PG - 1 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/34012221 ID - 34012221 LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Aletta Kata AU - Varga, Árpád AU - Susánszki, Petra AU - Lázár, György ifj AU - Groma, Gergely AU - Pallagi, Petra AU - Maléth, József TI - Pressure-induced inflammatory processes are mediated by PIEZO1 in human pancreatic organoids JF - CENTRAL EUROPEAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY / GASZTROENTEROLÓGIAI ÉS HEPATOLÓGIAI SZEMLE J2 - CENT EUR J GASTRO HEPATOL VL - 9 PY - 2023 IS - Suppl. 1 SP - 86 EP - 87 PG - 2 SN - 2415-9107 UR - https://m2.mtmt.hu/api/publication/34012214 ID - 34012214 LA - English DB - MTMT ER -