TY - JOUR AU - Sinkó, Richárd AU - Mohácsik, Petra AU - Kővári, Dóra AU - Penksza, Veronika AU - Wittmann, Gábor AU - Borbélyné Mácsai, Lilla AU - Fonseca, Tatiana L AU - Bianco, Antonio Carlos AU - Fekete, Csaba AU - Gereben, Balázs TI - Different hypothalamic mechanisms control decreased circulating thyroid hormone levels in infection and fasting-induced Non-Thyroidal Illness Syndrome in male Thyroid Hormone Action Indicator Mice JF - THYROID J2 - THYROID VL - 33 PY - 2023 IS - 1 SP - 109 EP - 118 PG - 10 SN - 1050-7256 DO - 10.1089/thy.2022.0404 UR - https://m2.mtmt.hu/api/publication/33208795 ID - 33208795 LA - English DB - MTMT ER - TY - JOUR AU - Ruska, Yvette Magdolna AU - Stiftné Szilvásy-Szabó, Anett AU - Kővári, Dóra AU - Kádár, Andrea AU - Borbélyné Mácsai, Lilla AU - Sinkó, Richárd AU - Hrabovszky, Erik AU - Gereben, Balázs AU - Fekete, Csaba TI - Expression of glucagon-like peptide 1 receptor in neuropeptide Y neurons of the arcuate nucleus in mice JF - BRAIN STRUCTURE & FUNCTION J2 - BRAIN STRUCT FUNC VL - 227 PY - 2022 IS - 1 SP - 77 EP - 87 PG - 11 SN - 1863-2653 DO - 10.1007/s00429-021-02380-y UR - https://m2.mtmt.hu/api/publication/32287872 ID - 32287872 N1 - Laboratory of Integrative Neuroendocrinology, Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Laboratory of Molecular Cell Metabolism, Institute of Experimental Medicine, Budapest, 1083, Hungary Laboratory of Reproductive Neurobiology, Institute of Experimental Medicine, Budapest, 1083, Hungary Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Tupper Research Institute, Tufts Medical Center, Boston, MA 02111, United States Cited By :2 Export Date: 2 November 2023 Correspondence Address: Fekete, C.; Laboratory of Integrative Neuroendocrinology, Szigony Street 43, Hungary; email: fekete.csaba@koki.hu Molecular Sequence Numbers: GENBANK: NM021332; Chemicals/CAS: neuropeptide Y, 82785-45-3, 83589-17-7; glucagon like peptide 1, 89750-14-1; proopiomelanocortin, 66796-54-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Neuropeptide Y; Pro-Opiomelanocortin; RNA, Messenger Funding details: 2017–1.2.1-NKP-2017–00002 Funding details: Horizon 2020 Framework Programme, H2020, 666869 Funding details: Hungarian Scientific Research Fund, OTKA, K124767, K128317 Funding details: Novo Nordisk Funding details: Hungarian Science Foundation Funding text 1: C.F. received funding from Novo Nordisk A/S. Y.R., A.Sz-Sz., D.K., A.K., L.M., R.S., E.H., and B.G. declare no competing interest. Funding text 2: This work was supported by Grants from the Hungarian Science Foundation (OTKA K124767 and K128317), Hungarian National Brain Research Program (2017–1.2.1-NKP-2017–00002), EU H2020 THYRAGE no. 666869. LA - English DB - MTMT ER - TY - THES AU - Borbélyné Mácsai, Lilla TI - Specially regulated electron deprivation extremely prolongs lifespan and delays aging in middle-aged rotifers [Speciálisan szabályozott elektron depriváció hatása az élettartam növelésére és az öregedés késleltetésére középkorú rotifereknél] PB - Szegedi Tudományegyetem (SZTE) PY - 2020 SP - 52 DO - 10.14232/phd.10388 UR - https://m2.mtmt.hu/api/publication/31329798 ID - 31329798 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Borbélyné Mácsai, Lilla AU - Pákáski, Magdolna AU - Gálik, Bence AU - Mihaly, Gabor AU - Kálmán, János TI - Application of BisANS fluorescent dye for developing a novel protein assay JF - PLOS ONE J2 - PLOS ONE VL - 14 PY - 2019 IS - 4 PG - 8 SN - 1932-6203 DO - 10.1371/journal.pone.0215863 UR - https://m2.mtmt.hu/api/publication/30812390 ID - 30812390 LA - English DB - MTMT ER - TY - JOUR AU - Borbélyné Mácsai, Lilla AU - Oláh, Zita AU - Bush, Ashley I AU - Gálik, Bence AU - Ónody, Rita AU - Kálmán, János AU - Datki, Zsolt László TI - Redox Modulating Factors Affect Longevity Regulation in Rotifers JF - JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES J2 - J GERONTOL A-BIOL MED SCI VL - 74 PY - 2019 IS - 6 SP - 811 EP - 814 PG - 4 SN - 1079-5006 DO - 10.1093/gerona/gly193 UR - https://m2.mtmt.hu/api/publication/30626843 ID - 30626843 LA - English DB - MTMT ER - TY - JOUR AU - Chuluunbaatar, Bayar AU - Béni, Zoltán AU - Dékány, Miklós AU - Kovács, Bernadett AU - Sárközy, András AU - Datki, Zsolt László AU - Borbélyné Mácsai, Lilla AU - Kálmán, János AU - Hohmann, Judit AU - Ványolós, Attila TI - Triterpenes from the Mushroom Hypholoma lateritium: Isolation, Structure Determination and Investigation in Bdelloid Rotifer Assays JF - MOLECULES J2 - MOLECULES VL - 24 PY - 2019 IS - 2 PG - 10 SN - 1420-3049 DO - 10.3390/molecules24020301 UR - https://m2.mtmt.hu/api/publication/30518916 ID - 30518916 N1 - Funding Agency and Grant Number: GINOP research program [2.3.2-15-2016-00012]; European Regional Development Fund; Hungarian National Research, Development and Innovation Fund [PD 124476] Funding text: This work was supported by the GINOP research program (project no. 2.3.2-15-2016-00012) funded by the European Regional Development Fund. A. Vanyolos is grateful for the grant of the Hungarian National Research, Development and Innovation Fund (PD 124476). Department of Pharmacognosy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Spectroscopic Research, Gedeon Richter Plc, Gyömr oi út 19-21, Budapest, H-1103, Hungary Department of Psychiatry, Faculty of Medicine, University of Szeged, Kálvária sgt. 57, Szeged, H-6725, Hungary Interdisciplinary Centre for Natural Products, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Cited By :2 Export Date: 28 August 2019 CODEN: MOLEF Correspondence Address: Ványolós, A.; Department of Pharmacognosy, University of Szeged, Eötvös u. 6, Hungary; email: vanyolosa@pharmacognosy.hu Chemicals/CAS: Triterpenes Funding details: European Regional Development Fund Funding details: PD 124476 Funding text 1: Funding: This work was supported by the GINOP research program (project no. 2.3.2-15-2016-00012) funded by the European Regional Development Fund. A. Ványolós is grateful for the grant of the Hungarian National Research, Development and Innovation Fund (PD 124476). Funding Agency and Grant Number: GINOP research program [2.3.2-15-2016-00012]; European Regional Development FundEuropean Union (EU); Hungarian National Research, Development and Innovation Fund [PD 124476] Funding text: This work was supported by the GINOP research program (project no. 2.3.2-15-2016-00012) funded by the European Regional Development Fund. A. Vanyolos is grateful for the grant of the Hungarian National Research, Development and Innovation Fund (PD 124476). Cited By :2 Export Date: 3 December 2019 CODEN: MOLEF Correspondence Address: Ványolós, A.; Department of Pharmacognosy, University of Szeged, Eötvös u. 6, Hungary; email: vanyolosa@pharmacognosy.hu AB - Twelve compounds (1-12) were isolated from the methanol extract of brick cap mushroom (Hypholoma lateritium (Schaeff.) P. Kumm.). The structures of the compounds were elucidated using extensive spectroscopic analyses, including NMR and MS measurements. Lanosta-7,9(11)-diene-12,21-epoxy-2,3,24,25-tetraol (1) and 8-hydroxy-13-oxo-9E,11E-octa-decadienoic acid (2) were identified as new natural products, together with ten known compounds, from which 3-hydroxyergosta-7,22-diene (4), demethylincisterol A2 (5), cerevisterol (6), 3-O-glucopyranosyl-5,8-epidioxyergosta-6,22-diene (7), fasciculol E (9), and uridine (12) were identified in this species for the first time. The isolated triterpenes (1, 3-11) were investigated for their toxicity in vivo using bdelloid rotifer assays. Most of the examined steroids in general showed low toxicity, although the effects of the compounds varied in a wider range from the non-toxic lanosta-7,9(11)-diene-12,21-epoxy-2,3,24,25-tetraol (1) to the significantly toxic cerevisterol (6), with substantial dependence in some cases on the presence of nutrient in the experimental environment. LA - English DB - MTMT ER - TY - JOUR AU - Borbélyné Mácsai, Lilla AU - Datki, Zsolt László AU - Csupor, Dezső AU - Horváth, Attila AU - Zomborszki, Zoltán Péter TI - Biological activities of four adaptogenic plant extracts and their active substances on a rotifer model JF - EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE J2 - EVID-BASED COMPL ALT VL - 2018 PY - 2018 PG - 4 SN - 1741-427X DO - 10.1155/2018/3690683 UR - https://m2.mtmt.hu/api/publication/30323215 ID - 30323215 AB - Rotifers have been widely used as well-characterized models of aging, since their multiorgan character makes them suitable as in vivo toxicological and lifespan models. Here we report the assessment of four adaptogenic plants and their extracts for the first time in this model. The effects on rotifer viability of extracts and characteristic active markers of Panax ginseng, Withania somnifera, Leuzea carthamoides, and Rhodiola rosea were tested in vivo. The crude extracts were nontoxic to Philodina acuticornis bdelloid rotifers; however, the pure substances of the plants influenced negatively the viability. Ginsenoside Rb1 and secondary metabolites of Withania somnifera exerted deleterious effect on the animals. The aglycone tyrosol and cinnamyl alcohol (from Rhodiola rosea) were more toxic than their glycosides salidroside and rosavin. Although the 20-OH-ecdysone and ajugasterone C (from Leuzea carthamoides) are chemically very similar, the latter was less toxic. LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Hortobágyi, Tibor AU - Borbélyné Mácsai, Lilla AU - Zsuga, Katalin AU - Fülöp, Lívia AU - Bozsó, Zsolt AU - Gálik, Bence AU - Ács, Éva AU - Földi, Angéla AU - Szarvas, A AU - Kálmán, János TI - Exceptional in vivo catabolism of neurodegeneration-related aggregates JF - ACTA NEUROPATHOLOGICA COMMUNICATIONS J2 - ACTA NEUROPATH COMM VL - 6 PY - 2018 IS - 1 PG - 12 SN - 2051-5960 DO - 10.1186/s40478-018-0507-3 UR - https://m2.mtmt.hu/api/publication/3323000 ID - 3323000 LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Tivadar AU - Borbélyné Mácsai, Lilla AU - Csupor, Dezső AU - Datki, Zsolt László TI - In vivo screening of diterpene alkaloids using bdelloid rotifer assays JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 68 PY - 2017 IS - 4 SP - 443 EP - 452 PG - 10 SN - 0236-5383 DO - 10.1556/018.68.2017.4.9 UR - https://m2.mtmt.hu/api/publication/3307542 ID - 3307542 AB - The group of diterpene alkaloids contains numerous compounds with complex chemistry and diverse pharmacological activities. Beside toxicity, these compounds possess activity on the cardiovascular system, tumor cell lines and nervous system. The pharmacological properties have been described using in vitro and in vivo techniques; however, the bioactivities of many compounds have not thoroughly been studied. Here we report on the in vivo evaluation of ten diterpene alkaloids using bdelloid rotifer assays. Napelline exerted toxic effects on rotifers, while wide tolerance range was observed for other investigated compounds. Weak toxicity of songorine is supported by our experiment. Toxicological data for senbusine A, senbusine C, septentrioidine and hetisinone are reported for the first time. LA - English DB - MTMT ER - TY - JOUR AU - Oláh, Zita AU - Bush, AI AU - Aleksza, Dávid AU - Gálik, Bence AU - Ivitz, Eszter Virág AU - Borbélyné Mácsai, Lilla AU - Janka, Zoltán AU - Kármán, Zoltán AU - Kálmán, János AU - Datki, Zsolt László TI - Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 144 PY - 2017 SP - 115 EP - 122 PG - 8 SN - 0147-6513 DO - 10.1016/j.ecoenv.2017.06.005 UR - https://m2.mtmt.hu/api/publication/3241020 ID - 3241020 N1 - Department of Psychiatry, University of Szeged, Szeged, Hungary Oxidation Biology Unit, Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Bioinformatics & Scientific Computing, Vienna Biocenter Core Facilities, Vienna, Austria Cited By :6 Export Date: 24 September 2019 CODEN: EESAD Correspondence Address: Datki, Z.; Department of Psychiatry, University of Szeged, Kalvaria Sgt. 57, 6725, Hungary; email: datkizsolt@gmail.com Chemicals/CAS: hydrogen peroxide, 7722-84-1; sodium azide, 26628-22-8; Hydrogen Peroxide; RNA, Ribosomal, 18S; Sodium Azide AB - Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H2O2) and sodium azide (NaN3) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science. LA - English DB - MTMT ER -