@article{MTMT:34836454,
	title = {Short-term hyperoxia-induced functional and morphological changes in rat hippocampus},
	url = {https://m2.mtmt.hu/api/publication/34836454},
	author = {Hencz, Alexandra Júlia and Magony, Andor Dániel and Thomas, Chloe and Kovacs, Krisztina and Szilágyi, Tamás Gábor and Pál, József and Sík, Attila},
	doi = {10.3389/fncel.2024.1376577},
	journal-iso = {FRONT CELL NEUROSCI},
	journal = {FRONTIERS IN CELLULAR NEUROSCIENCE},
	volume = {18},
	unique-id = {34836454},
	issn = {1662-5102},
	abstract = {Excess oxygen (O2) levels may have a stimulating effect, but in the long term, and at high concentrations of O2, it is harmful to the nervous system. The hippocampus is very sensitive to pathophysiological changes and altered O2 concentrations can interfere with hippocampus-dependent learning and memory functions. In this study, we investigated the hyperoxia-induced changes in the rat hippocampus to evaluate the short-term effect of mild and severe hyperoxia. Wistar male rats were randomly divided into control (21% O2), mild hyperoxia (30% O2), and severe hyperoxia groups (100% O2). The O2 exposure lasted for 60 min. Multi-channel silicon probes were used to study network oscillations and firing properties of hippocampal putative inhibitory and excitatory neurons. Neural damage was assessed using the Gallyas silver impregnation method. Mild hyperoxia (30% O2) led to the formation of moderate numbers of silver-impregnated "dark" neurons in the hippocampus. On the other hand, exposure to 100% O2 was associated with a significant increase in the number of "dark" neurons located mostly in the hilus. The peak frequency of the delta oscillation decreased significantly in both mild and severe hyperoxia in urethane anesthetized rats. Compared to normoxia, the firing activity of pyramidal neurons under hyperoxia increased while it was more heterogeneous in putative interneurons in the cornu ammonis area 1 (CA1) and area 3 (CA3). These results indicate that short-term hyperoxia can change the firing properties of hippocampal neurons and network oscillations and damage neurons. Therefore, the use of elevated O2 concentration inhalation in hospitals (i.e., COVID treatment and surgery) and in various non-medical scenarios (i.e., airplane emergency O2 masks, fire-fighters, and high altitude trekkers) must be used with extreme caution.},
	keywords = {hippocampus; Electrophysiology; HYPEROXIA; network oscillation; Dark neuron},
	year = {2024},
	eissn = {1662-5102}
}

@article{MTMT:34173277,
	title = {Mild hypoxia-induced structural and functional changes of the hippocampal network},
	url = {https://m2.mtmt.hu/api/publication/34173277},
	author = {Hencz, Alexandra Júlia and Magony, Andor Dániel and Thomas, Chloe and Kovacs, Krisztina and Szilágyi, Tamás Gábor and Pál, József and Sík, Attila},
	doi = {10.3389/fncel.2023.1277375},
	journal-iso = {FRONT CELL NEUROSCI},
	journal = {FRONTIERS IN CELLULAR NEUROSCIENCE},
	volume = {17},
	unique-id = {34173277},
	issn = {1662-5102},
	abstract = {Hypoxia causes structural and functional changes in several brain regions, including the oxygen-concentration-sensitive hippocampus. We investigated the consequences of mild short-term hypoxia on rat hippocampus in vivo. The hypoxic group was treated with 16% O2 for 1 h, and the control group with 21% O2. Using a combination of Gallyas silver impregnation histochemistry revealing damaged neurons and interneuron-specific immunohistochemistry, we found that somatostatin-expressing inhibitory neurons in the hilus were injured. We used 32-channel silicon probe arrays to record network oscillations and unit activity from the hippocampal layers under anaesthesia. There were no changes in the frequency power of slow, theta, beta, or gamma bands, but we found a significant increase in the frequency of slow oscillation (2.1–2.2 Hz) at 16% O2 compared to 21% O2. In the hilus region, the firing frequency of unidentified interneurons decreased. In the CA3 region, the firing frequency of some unidentified interneurons decreased while the activity of other interneurons increased. The activity of pyramidal cells increased both in the CA1 and CA3 regions. In addition, the regularity of CA1, CA3 pyramidal cells’ and CA3 type II and hilar interneuron activity has significantly changed in hypoxic conditions. In summary, a low O2 environment caused profound changes in the state of hippocampal excitatory and inhibitory neurons and network activity, indicating potential changes in information processing caused by mild short-term hypoxia.},
	year = {2023},
	eissn = {1662-5102}
}

@article{MTMT:32099381,
	title = {Correlations between Total Antioxidant Capacity, Polyphenol and Fatty Acid Content of Native Grape Seed and Pomace of Four Different Grape Varieties in Hungary},
	url = {https://m2.mtmt.hu/api/publication/32099381},
	author = {Szabó, Éva and Marosvölgyi, Tamás and Szilágyi, Tamás Gábor and Kőrösi, László Tamás and Schmidt, János and Csepregi, Kristóf and Márk, László and Bóna, Ágnes},
	doi = {10.3390/antiox10071101},
	journal-iso = {ANTIOXIDANTS-BASEL},
	journal = {ANTIOXIDANTS},
	volume = {10},
	unique-id = {32099381},
	abstract = {Grape pomace is a valuable source of various bioactive compounds such as plant-derived polyphenols and polyunsaturated fatty acids (PUFAs). The commercial demand of grape skin and seed powders as nutraceuticals is still growing. However, no distinction is currently made between unfermented native grape seed and grape seed pomace powders regarding their antioxidant activities. Our aim was to find the relationship between the polyphenol and fatty acid content as well as the antioxidant capacity of native and fermented grape seeds of four different grape varieties harvested in the Villány wine region. According to our results, none of the three investigated polyphenols (resveratrol, rutin, quercetin) could be detected in native grape seed samples in correlation with their significantly lower total antioxidant capacities compared to fermented seed samples. Pinot Noir (PN) grape seed pomace samples with the highest resveratrol and oil content showed significantly higher total antioxidant capacity than Cabernet Sauvignon (CS), Syrah (S) and Blue Portugal (BP) samples. Based on the statistical analysis, positive correlation was found between the fatty acid content and the resveratrol concentration in the pomace samples of different grape varieties. In contrast, rutin concentrations were negatively proportional to the fatty acid content of the fermented samples. No significant correlation was found considering the quercetin content of the samples. According to our findings, grape pomace seems a more promising source in the production of nutraceuticals, since it contains polyphenols in higher concentration and exerts significantly higher antioxidant activity than native grape seeds.
		
		Keywords: grape seed; pomace; antioxidant activity; polyunsaturated fatty acids; polyphenol; resveratrol; rutin; HPLC; GC},
	year = {2021},
	eissn = {2076-3921},
	orcid-numbers = {Szabó, Éva/0000-0002-3563-2492; Marosvölgyi, Tamás/0000-0002-4244-5513; Kőrösi, László Tamás/0000-0003-3138-6041; Márk, László/0000-0002-9301-8159}
}

@article{MTMT:31277884,
	title = {Proteomic changes during experimental de- and remyelination in the corpus callosum},
	url = {https://m2.mtmt.hu/api/publication/31277884},
	author = {Szilágyi, Tamás Gábor and Nawrocki, Arkadiusz M and Erős, Krisztián and Schmidt, János and Fekete, Katalin and Elkjaer, Maria L and Hyrlov, Kirsten H and Larsen, Martin R and Illés, Zsolt László and Gallyas, Ferenc},
	doi = {10.1371/journal.pone.0230249},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {15},
	unique-id = {31277884},
	issn = {1932-6203},
	abstract = {In the cuprizone model of multiple sclerosis, de- and remyelination can be studied without major interference from the adaptive immune responses. Since previous proteomic studies did not focus on the corpus callosum, where cuprizone causes the most pronounced demyelination, we performed a bottom up proteomic analysis on this brain region.Eight week-old mice treated with 0.2% cuprizone, for 4 weeks and controls (C) were sacrificed after termination of the treatment (4wD), and 2 (2dR) or 14 (2wR) days later. Homogenates of dissected corpus callosum were analysed by quantitative proteomics. For data processing, clustering, gene ontology analysis, and regulatory network prediction, we used Perseus, PANTHER and Ingenuity Pathway Analysis softwares, respectively.We identified 4886 unmodified, single- or multi phosphorylated and/or gycosylated (PTM) proteins. Out of them, 191 proteins were differentially regulated in at least one experimental group. We found 57 proteins specific for demyelination, 27 for early- and 57 for late remyelinationwhile 36 proteins were affected in two, and 23 proteins in all three groups. Phosphorylation represented 92% of the post translational modifications among differentially regulated modified (PTM) proteins with decreased level, while it was only 30% of the PTM proteins with increased level. Gene ontology analysis could not classify the demyelination specific proteins into any biological process category, while allocated the remyelination specific ones to nervous system development and myelination as the most specific subcategory. We also identified a protein network in experimental remyelination, and the gene orthologues of the network were differentially expressed in remyelinating multiple sclerosis brain lesions consistent with an early remyelination pattern.Proteomic analysis seems more informative for remyelination than demyelination in the cuprizone model.},
	year = {2020},
	eissn = {1932-6203},
	orcid-numbers = {Illés, Zsolt László/0000-0001-9655-0450; Gallyas, Ferenc/0000-0002-1906-4333}
}

@article{MTMT:3352238,
	title = {Experimental demyelination and axonal loss are reduced in MicroRNA-146a deficient mice},
	url = {https://m2.mtmt.hu/api/publication/3352238},
	author = {Martin, NA and Molnár, Viktor and Szilágyi, Tamás Gábor and Elkjaer, ML and Nawrocki, A and Okarmus, J and Wlodarczyk, A and Thygesen, EK and Palkovits, Miklós and Gallyas, Ferenc and Larsen, MR and Lassmann, H and Benedikz, E and Owens, T and Svenningsen, AF and Illés, Zsolt László},
	doi = {10.3389/fimmu.2018.00490},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {9},
	unique-id = {3352238},
	issn = {1664-3224},
	year = {2018},
	eissn = {1664-3224},
	orcid-numbers = {Molnár, Viktor/0000-0002-4156-9987; Palkovits, Miklós/0000-0003-0578-0387; Gallyas, Ferenc/0000-0002-1906-4333; Illés, Zsolt László/0000-0001-9655-0450}
}

@misc{MTMT:3260841,
	title = {Regulation of de- and remyelination in the central nervous system},
	url = {https://m2.mtmt.hu/api/publication/3260841},
	author = {Szilágyi, Tamás Gábor and Arkadiusz, M Nawrocki and Schmidt, János and Ferenc, Gallyas Jr and Zsolt, Illes},
	unique-id = {3260841},
	year = {2016}
}

@book{MTMT:3072069,
	title = {5th Interdisciplinary Doctoral Conference : Book of abstracts =V. Interdiszciplináris Doktorandusz Konferencia : Absztraktkötet},
	url = {https://m2.mtmt.hu/api/publication/3072069},
	isbn = {9789634290377},
	editor = {Ács, Kamilla and Bencze, Noémi and Bódog, Ferenc and Haffner, Tamás and Hegyi, Dávid and Horváth, Orsolya Melinda and Hüber, Gabriella Margit and Kovács, Áron and Kis Kelemen, Bence and Lajkó, Adrienn and Schilli, Gabriella Krisztina and Stankovicsné Szendi, Anna and Szilágyi, Tamás Gábor and Varga, Zoltán},
	publisher = {Doctoral Student Association of the University of Pécs},
	unique-id = {3072069},
	year = {2016},
	orcid-numbers = {Varga, Zoltán/0000-0002-2494-6664}
}