TY - JOUR AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Szabó, Viktória AU - Takács, Tamás AU - Németh, Margit AU - Farkas, Gyula Jr. AU - Szederkényi, Edit AU - Hegyi, Péter AU - Pallagi, Petra AU - Maléth, József TI - Functional Characterization of Human Pancreatic Ductal Organoids in Controlled Polarity Models JF - PANCREAS J2 - PANCREAS VL - 50 PY - 2021 IS - 7 SP - 1078 EP - 1078 PG - 1 SN - 0885-3177 UR - https://m2.mtmt.hu/api/publication/32470641 ID - 32470641 LA - English DB - MTMT ER - TY - CONF AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Szabó, Viktória AU - Németh, Margit AU - Farkas, Gyula Jr. AU - Szederkényi, Edit AU - Lázár, György ifj AU - Pallagi, Petra AU - Maléth, József AU - Takács, Tamás TI - Characterization of bicarbonate secretion in human pancreatic ductal organoid cultures T2 - EMBO/EMBL Symposium: Organoids: Modelling Organ Development and Disease in 3D Culture PY - 2020 PG - 1 UR - https://m2.mtmt.hu/api/publication/32592522 ID - 32592522 LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Réka AU - Madácsy, Tamara AU - Varga, Árpád AU - Németh, Margit AU - Katona, Xénia AU - Görög, Marietta AU - Molnár, Brigitta AU - Fanczal, Júlia AU - Rakonczay, Zoltán AU - Hegyi, Péter AU - Pallagi, Petra AU - Maléth, József TI - Mouse pancreatic ductal organoid culture as a relevant model to study exocrine pancreatic ion secretion JF - LABORATORY INVESTIGATION J2 - LAB INVEST VL - 100 PY - 2020 IS - 1 SP - 84 EP - 97 PG - 14 SN - 0023-6837 DO - 10.1038/s41374-019-0300-3 UR - https://m2.mtmt.hu/api/publication/30766190 ID - 30766190 N1 - First Department of Medicine, University of Szeged, Szeged, Hungary HAS-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, University of Szeged, Szeged, Hungary Department of Pathophysiology, University of Szeged, Szeged, Hungary HAS-USZ Momentum Translational Gastroenterology Research Group, University of Szeged, Szeged, Hungary Institute for Translational Medicine and First Department Medicine, Medical School, University of Pécs, Pécs, Hungary Department of Public Health, University of Szeged, Szeged, Hungary Cited By :22 Export Date: 26 January 2024 CODEN: LAINA Correspondence Address: Maléth, J.; First Department of Medicine, Hungary; email: maleth.jozsef@med.u-szeged-hu AB - Pancreatic exocrine secretory processes are challenging to investigate on primary epithelial cells. Pancreatic organoid cultures may help to overcome shortcomings of the current models, however the ion secretory processes in pancreatic organoids-and therefore their physiological relevance or their utility in disease modeling-are not known. To answer these questions, we provide side-by-side comparison of gene expression, morphology, and function of epithelial cells in primary isolated pancreatic ducts and organoids. We used mouse pancreatic ductal fragments for experiments or were grown in Matrigel to obtain organoid cultures. Using PCR analysis we showed that gene expression of ion channels and transporters remarkably overlap in primary ductal cells and organoids. Morphological analysis with scanning electron microscopy revealed that pancreatic organoids form polarized monolayers with brush border on the apical membrane. Whereas the expression and localization of key proteins involved in ductal secretion (cystic fibrosis transmembrane conductance regulator, Na+/H+ exchanger 1 and electrogenic Na+/HCO3- cotransporter 1) are equivalent to the primary ductal fragments. Measurements of intracellular pH and Cl- levels revealed no significant difference in the activities of the apical Cl-/HCO3- exchange, or in the basolateral Na+ dependent HCO3- uptake. In summary we found that ion transport activities in the mouse pancreatic organoids are remarkably similar to those observed in freshly isolated primary ductal fragments. These results suggest that organoids can be suitable and robust model to study pancreatic ductal epithelial ion transport in health and diseases and facilitate drug development for secretory pancreatic disorders like cystic fibrosis, or chronic pancreatitis. LA - English DB - MTMT ER - TY - JOUR AU - Németh, Margit AU - Varga, Árpád AU - Katona, Xénia AU - Madácsy, Tamara AU - Takács, Tamás AU - Farkas, Gyula Jr. AU - Szederkényi, Edit AU - Lázár, György ifj AU - Hegyi, Péter AU - Pallagi, Petra AU - Maléth, József TI - Investigation of bicarbonate secretion in human ductal organoid cell cultures JF - PANCREATOLOGY J2 - PANCREATOLOGY VL - 19 PY - 2019 IS - Suppl 1 SP - S93 SN - 1424-3903 DO - 10.1016/j.pan.2019.05.248 UR - https://m2.mtmt.hu/api/publication/31627389 ID - 31627389 LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Réka AU - Görög, Marietta AU - Fanczal, Júlia AU - Madácsy, Tamara AU - Németh, Margit AU - Varga, Árpád AU - Katona, Xénia AU - Rakonczay, Zoltán AU - Hegyi, Péter AU - Pallagi, Petra AU - Maléth, József TI - Pancreatic ductal organoid cultures are a suitable model to studypancreatic ductal ion secretion JF - PANCREATOLOGY J2 - PANCREATOLOGY VL - 19 PY - 2019 IS - Suppl 1 SP - S18 EP - S18 PG - 1 SN - 1424-3903 DO - 10.1016/j.pan.2019.05.039 UR - https://m2.mtmt.hu/api/publication/31398797 ID - 31398797 LA - English DB - MTMT ER - TY - JOUR AU - Bognár, Anna AU - Csete, Gergő AU - Németh, Margit AU - Csibri, Péter AU - Kincses, Zsigmond Tamás AU - Sáry, Gyula TI - Transcranial Stimulation of the Orbitofrontal Cortex Affects Decisions about Magnocellular Optimized Stimuli JF - FRONTIERS IN NEUROSCIENCE J2 - FRONT NEUROSCI-SWITZ VL - 11 PY - 2017 PG - 11 SN - 1662-4548 DO - 10.3389/fnins.2017.00234 UR - https://m2.mtmt.hu/api/publication/3213703 ID - 3213703 N1 - Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :3 Export Date: 12 August 2020 Correspondence Address: Sáry, G.; Department of Physiology, University of SzegedHungary; email: sary.gyula@med.u-szeged.hu Funding details: European Commission, EC Funding details: CZ.1.05/1.1.00/02.0123 Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Magyar Tudományos Akadémia, MTA Funding details: Hungarian Scientific Research Fund, OTKA, PD 104715, K83671 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis Funding Agency and Grant Number: Neuroscience Research Group of the Hungarian Academy of Sciences; University of Szeged; OTKAOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [PD 104715, K83671]; European Regional Development Fund-Project FNUSA-ICRC [CZ.1.05/1.1.00/02.0123]; European Union - project ICRC-ERA-Human Bridge [316345]; European UnionEuropean Union (EU); State of Hungary; European Social FundEuropean Social Fund (ESF) [TAMOP 4.2.4.A/2-11-1-2012-0001]; Human Capacities Grant Management Office [NTP-NFTO-16]; Hungarian Ministry of Human Capacities; National Brain Research Program [KTIA_13_NAP-A-11/20] Funding text: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-11/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union - project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFTO-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and E. Demeter for proofreading the manuscript and F. Rarosi for helping with the statistical analysis. Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :4 Export Date: 28 March 2021 Correspondence Address: Sáry, G.; Department of Physiology, Hungary; email: sary.gyula@med.u-szeged.hu Funding details: CZ.1.05/1.1.00/02.0123 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, K83671, PD 104715 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Szegedi Tudományegyetem, SZTE Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis Funding Agency and Grant Number: Neuroscience Research Group of the Hungarian Academy of Sciences; University of SzegedEuropean Commission; OTKAOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [PD 104715, K83671]; European Regional Development Fund-Project FNUSA-ICRC [CZ.1.05/1.1.00/02.0123]; European Union - project ICRC-ERA-Human Bridge [316345]; European UnionEuropean Commission; State of Hungary; European Social FundEuropean Social Fund (ESF) [TAMOP 4.2.4.A/2-11-1-2012-0001]; Human Capacities Grant Management Office [NTP-NFTO-16]; Hungarian Ministry of Human Capacities; National Brain Research Program [KTIA_13_NAP-A-11/20] Funding text: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-11/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union - project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFTO-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and E. Demeter for proofreading the manuscript and F. Rarosi for helping with the statistical analysis. Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :5 Export Date: 8 September 2021 Correspondence Address: Sáry, G.; Department of Physiology, Hungary; email: sary.gyula@med.u-szeged.hu Funding details: CZ.1.05/1.1.00/02.0123 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, K83671, PD 104715 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Szegedi Tudományegyetem, SZTE Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :5 Export Date: 21 September 2021 Correspondence Address: Sáry, G.; Department of Physiology, Hungary; email: sary.gyula@med.u-szeged.hu Funding details: CZ.1.05/1.1.00/02.0123 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, K83671, PD 104715 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Szegedi Tudományegyetem, SZTE Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :5 Export Date: 22 September 2021 Correspondence Address: Sáry, G.; Department of Physiology, Hungary; email: sary.gyula@med.u-szeged.hu Funding details: CZ.1.05/1.1.00/02.0123 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, K83671, PD 104715 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Szegedi Tudományegyetem, SZTE Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis Department of Physiology, University of Szeged, Szeged, Hungary Department of Neurology, University of Szeged, Szeged, Hungary Department of Anaesthesiology and Intensive Therapy, University of Szeged, Szeged, Hungary Cited By :5 Export Date: 23 September 2021 Correspondence Address: Sáry, G.; Department of Physiology, Hungary; email: sary.gyula@med.u-szeged.hu Funding details: CZ.1.05/1.1.00/02.0123 Funding details: 316345 Funding details: KTIA_13_NAP-A-II/20 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, K83671, PD 104715 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Social Fund, ESF, A/2-11-1-2012-0001 Funding details: Szegedi Tudományegyetem, SZTE Funding text 1: The study was supported by the Neuroscience Research Group of the Hungarian Academy of Sciences and the University of Szeged, the National Brain Research Program (Grant No. KTIA_13_NAP-A-II/20.), OTKA [PD 104715] and OTKA [K83671] grants. TK was supported by the European Regional Development Fund-Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123) and by the European Union-project ICRC-ERA-Human Bridge (No. 316345). AB was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP 4.2.4.A/2-11-1-2012-0001 'National Excellence Program'. GCs was supported by the NTP-NFT?-16 project by the Human Capacities Grant Management Office and the Hungarian Ministry of Human Capacities. We thank J. Navracsics, V. Varga, and ?. Demeter for proofreading the manuscript and F. R?rosi for helping with the statistical analysis LA - English DB - MTMT ER -