@article{MTMT:32470641, title = {Functional Characterization of Human Pancreatic Ductal Organoids in Controlled Polarity Models}, url = {https://m2.mtmt.hu/api/publication/32470641}, author = {Madácsy, Tamara and Varga, Árpád and Kiss, Aletta Kata and Szabó, Viktória and Takács, Tamás and Németh, Margit and Farkas, Gyula Jr. and Szederkényi, Edit and Hegyi, Péter and Pallagi, Petra and Maléth, József}, journal-iso = {PANCREAS}, journal = {PANCREAS}, volume = {50}, unique-id = {32470641}, issn = {0885-3177}, year = {2021}, eissn = {1536-4828}, pages = {1078-1078}, orcid-numbers = {Madácsy, Tamara/0000-0001-5598-9723; Varga, Árpád/0000-0002-2379-139X; Kiss, Aletta Kata/0000-0001-6404-1555; Szabó, Viktória/0000-0001-6547-9102; Hegyi, Péter/0000-0003-0399-7259; Pallagi, Petra/0000-0001-8906-0840; Maléth, József/0000-0001-5768-3090} } @CONFERENCE{MTMT:32592522, title = {Characterization of bicarbonate secretion in human pancreatic ductal organoid cultures}, url = {https://m2.mtmt.hu/api/publication/32592522}, author = {Madácsy, Tamara and Varga, Árpád and Kiss, Aletta Kata and Szabó, Viktória and Németh, Margit and Farkas, Gyula Jr. and Szederkényi, Edit and Lázár, György ifj and Pallagi, Petra and Maléth, József and Takács, Tamás}, booktitle = {EMBO/EMBL Symposium: Organoids: Modelling Organ Development and Disease in 3D Culture}, unique-id = {32592522}, year = {2020}, orcid-numbers = {Madácsy, Tamara/0000-0001-5598-9723; Varga, Árpád/0000-0002-2379-139X; Kiss, Aletta Kata/0000-0001-6404-1555; Szabó, Viktória/0000-0001-6547-9102; Lázár, György ifj/0000-0001-7155-2978; Pallagi, Petra/0000-0001-8906-0840; Maléth, József/0000-0001-5768-3090} } @article{MTMT:30766190, title = {Mouse pancreatic ductal organoid culture as a relevant model to study exocrine pancreatic ion secretion}, url = {https://m2.mtmt.hu/api/publication/30766190}, author = {Molnár, Réka and Madácsy, Tamara and Varga, Árpád and Németh, Margit and Katona, Xénia and Görög, Marietta and Molnár, Brigitta and Fanczal, Júlia and Rakonczay, Zoltán and Hegyi, Péter and Pallagi, Petra and Maléth, József}, doi = {10.1038/s41374-019-0300-3}, journal-iso = {LAB INVEST}, journal = {LABORATORY INVESTIGATION}, volume = {100}, unique-id = {30766190}, issn = {0023-6837}, abstract = {Pancreatic exocrine secretory processes are challenging to investigate on primary epithelial cells. Pancreatic organoid cultures may help to overcome shortcomings of the current models, however the ion secretory processes in pancreatic organoids-and therefore their physiological relevance or their utility in disease modeling-are not known. To answer these questions, we provide side-by-side comparison of gene expression, morphology, and function of epithelial cells in primary isolated pancreatic ducts and organoids. We used mouse pancreatic ductal fragments for experiments or were grown in Matrigel to obtain organoid cultures. Using PCR analysis we showed that gene expression of ion channels and transporters remarkably overlap in primary ductal cells and organoids. Morphological analysis with scanning electron microscopy revealed that pancreatic organoids form polarized monolayers with brush border on the apical membrane. Whereas the expression and localization of key proteins involved in ductal secretion (cystic fibrosis transmembrane conductance regulator, Na+/H+ exchanger 1 and electrogenic Na+/HCO3- cotransporter 1) are equivalent to the primary ductal fragments. Measurements of intracellular pH and Cl- levels revealed no significant difference in the activities of the apical Cl-/HCO3- exchange, or in the basolateral Na+ dependent HCO3- uptake. In summary we found that ion transport activities in the mouse pancreatic organoids are remarkably similar to those observed in freshly isolated primary ductal fragments. These results suggest that organoids can be suitable and robust model to study pancreatic ductal epithelial ion transport in health and diseases and facilitate drug development for secretory pancreatic disorders like cystic fibrosis, or chronic pancreatitis.}, year = {2020}, eissn = {1530-0307}, pages = {84-97}, orcid-numbers = {Molnár, Réka/0000-0002-3128-825X; Madácsy, Tamara/0000-0001-5598-9723; Varga, Árpád/0000-0002-2379-139X; Katona, Xénia/0000-0002-6053-9097; Görög, Marietta/0000-0003-3615-3140; Fanczal, Júlia/0000-0001-7356-3857; Rakonczay, Zoltán/0000-0002-1499-3416; Hegyi, Péter/0000-0003-0399-7259; Pallagi, Petra/0000-0001-8906-0840; Maléth, József/0000-0001-5768-3090} } @article{MTMT:31627389, title = {Investigation of bicarbonate secretion in human ductal organoid cell cultures}, url = {https://m2.mtmt.hu/api/publication/31627389}, author = {Németh, Margit and Varga, Árpád and Katona, Xénia and Madácsy, Tamara and Takács, Tamás and Farkas, Gyula Jr. and Szederkényi, Edit and Lázár, György ifj and Hegyi, Péter and Pallagi, Petra and Maléth, József}, doi = {10.1016/j.pan.2019.05.248}, journal-iso = {PANCREATOLOGY}, journal = {PANCREATOLOGY}, volume = {19}, unique-id = {31627389}, issn = {1424-3903}, year = {2019}, eissn = {1424-3911}, pages = {S93}, orcid-numbers = {Varga, Árpád/0000-0002-2379-139X; Katona, Xénia/0000-0002-6053-9097; Madácsy, Tamara/0000-0001-5598-9723; Lázár, György ifj/0000-0001-7155-2978; Hegyi, Péter/0000-0003-0399-7259; Pallagi, Petra/0000-0001-8906-0840; Maléth, József/0000-0001-5768-3090} } @article{MTMT:31398797, title = {Pancreatic ductal organoid cultures are a suitable model to studypancreatic ductal ion secretion}, url = {https://m2.mtmt.hu/api/publication/31398797}, author = {Molnár, Réka and Görög, Marietta and Fanczal, Júlia and Madácsy, Tamara and Németh, Margit and Varga, Árpád and Katona, Xénia and Rakonczay, Zoltán and Hegyi, Péter and Pallagi, Petra and Maléth, József}, doi = {10.1016/j.pan.2019.05.039}, journal-iso = {PANCREATOLOGY}, journal = {PANCREATOLOGY}, volume = {19}, unique-id = {31398797}, issn = {1424-3903}, year = {2019}, eissn = {1424-3911}, pages = {S18-S18}, orcid-numbers = {Molnár, Réka/0000-0002-3128-825X; Görög, Marietta/0000-0003-3615-3140; Fanczal, Júlia/0000-0001-7356-3857; Madácsy, Tamara/0000-0001-5598-9723; Varga, Árpád/0000-0002-2379-139X; Katona, Xénia/0000-0002-6053-9097; Rakonczay, Zoltán/0000-0002-1499-3416; Hegyi, Péter/0000-0002-3128-825X; Pallagi, Petra/0000-0001-8906-0840; Maléth, József/0000-0001-5768-3090} } @article{MTMT:3213703, title = {Transcranial Stimulation of the Orbitofrontal Cortex Affects Decisions about Magnocellular Optimized Stimuli}, url = {https://m2.mtmt.hu/api/publication/3213703}, author = {Bognár, Anna and Csete, Gergő and Németh, Margit and Csibri, Péter and Kincses, Zsigmond Tamás and Sáry, Gyula}, doi = {10.3389/fnins.2017.00234}, journal-iso = {FRONT NEUROSCI-SWITZ}, journal = {FRONTIERS IN NEUROSCIENCE}, volume = {11}, unique-id = {3213703}, issn = {1662-4548}, year = {2017}, eissn = {1662-453X}, orcid-numbers = {Csete, Gergő/0000-0002-6440-5491; Kincses, Zsigmond Tamás/0000-0002-1442-4475} }