TY - JOUR AU - Mező, Emerencia AU - Balogh-Hartmann, Fruzsina AU - Madarászné Horváth, Ibolya AU - Bufa, Anita AU - Marosvölgyi, Tamás AU - Kocsis, Béla AU - Makszin, Lilla TI - Effect of Culture Conditions on Fatty Acid Profiles of Bacteria and Lipopolysaccharides of the Genus Pseudomonas—GC-MS Analysis on Ionic Liquid-Based Column JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 20 PG - 21 SN - 1420-3049 DO - 10.3390/molecules27206930 UR - https://m2.mtmt.hu/api/publication/33176667 ID - 33176667 AB - The profiling of bacterial fatty acids is a well-established technique in identifying and classifying bacteria. Cultivation conditions may affect the biosynthesis, thereby, changing the fatty acid profile in bacteria. The effect of the culture conditions on the fatty acid components of Pseudomonas aeruginosa PAO1, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa polyresistant and Pseudomonas putida all are aligned to the genus Pseudomonas. The fatty acids in the lipopolysaccharides of Pseudomonas aeruginosa PAO1 were also examined. The effects of the cultivation conditions were followed by using agar and blood agar media at the characteristic temperatures, 25 °C, 37 °C and 42 °C, respectively, and an analysis was made during the 1st, 3rd and 5th day following inoculation. In addition to quantitative differences, we also experienced qualitative differences in the fatty acid profiles which detect newly appearing fatty acids, due to changes in environmental factors. The application of ionic liquid-based column unveils new possibilities for the analyses of fatty acids in GC-MS experiments for bacterial fatty acid profiling. The validation results (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) show the high separation efficiency of the ionic liquid-based column in the analyses. LA - English DB - MTMT ER - TY - CHAP AU - Mező, Emerencia AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Balogh-Hartmann, Fruzsina AU - Kilár, Ferenc AU - Kocsis, Béla AU - Makszin, Lilla ED - Kajos, Luca Fanni ED - Bali, Cintia ED - Preisz, Zsolt ED - Polgár, Petra Ibolya ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám ED - Szabó, Rebeka TI - Környezeti tényezők változásainak hatása baktériumok zsírsavösszetételére T2 - 10th Jubilee Interdisciplinary Doctoral Conference : Book of Abstracts = 10. Jubileumi Interdiszciplináris Doktorandusz Konferencia : Absztraktkötet PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs SN - 9789634298205 PY - 2021 SP - 301 EP - 301 PG - 1 UR - https://m2.mtmt.hu/api/publication/32553805 ID - 32553805 N1 - Poszter LA - Hungarian DB - MTMT ER - TY - CHAP AU - Mező, Emerencia AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Balogh-Hartmann, Fruzsina AU - Kilár, Ferenc AU - Kocsis, Béla AU - Makszin, Lilla TI - Orvosi szempontból fontos baktériumok zsírsav-összetételének vizsgálata GC-MS módszerrel T2 - METT25 a Magyar Elválasztástudományi Társaság jubileumi konferenciája PB - Magyar Elválasztástudományi Társaság CY - Pécs SN - 9786155270666 PY - 2021 UR - https://m2.mtmt.hu/api/publication/32475734 ID - 32475734 LA - Hungarian DB - MTMT ER - TY - BOOK ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - Medical Conference for PhD Students and Experts of Clinical Sciences 2021. Book of Abstracts TS - Book of Abstracts PB - University of Pécs, Doctoral Student Association CY - Pécs PY - 2021 SP - 128 SN - 9789634296539 UR - https://m2.mtmt.hu/api/publication/32024807 ID - 32024807 LA - English DB - MTMT ER - TY - JOUR AU - Mező, Emerencia AU - Bufa, Anita AU - Páger, Csilla AU - Poór, Viktória AU - Marosvölgyi, Tamás AU - Kilár, Ferenc AU - Makszin, Lilla TI - The Role of Ionic Liquid Interaction in the Separation of Fatty Acid Methyl Esters—Polyunsaturated Geometric Isomers in GC–MS JF - SEPARATIONS J2 - SEPARATIONS VL - 8 PY - 2021 IS - 4 PG - 16 SN - 2297-8739 DO - 10.3390/separations8040038 UR - https://m2.mtmt.hu/api/publication/31933042 ID - 31933042 AB - Knowledge of the type and level of saturated and unsaturated fatty acids in food and clinical matrices is of practical importance, but the wide variety of fatty acids makes analyses very complex. The discrimination of the geometric isomers of fatty acid needs proper and effective separation conditions. The efficiency of three different stationary phases was evaluated by GC–MS methods in the separation of fatty acids in their methyl ester forms. Significant differences were observed in the efficiencies of polysiloxane-based (non-polar HP-5MS and medium/high polarity DB-225MS) and ionic liquid-based (SLB-IL111) columns. Baseline separation of the geometric isomers of linoleic acid methyl ester was obtained by the extremely polar SLB-IL111 column, showing a preference over the other two columns. The optimization of the experimental conditions (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) showed the separation power of the ionic liquid interaction in the analyses by using short (25–30 m long) columns. By deducting the general principles of the interaction, predictions can be made for the separation of other isomers. The results facilitate the precise identification of various types of fatty acids in real samples for nutritional information. LA - English DB - MTMT ER - TY - BOOK ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - IX. Interdiszciplináris Doktorandusz Konferencia 2020 [9th Interdisciplinary Doctoral Conference 2020]. Tanulmánykötet [Conference Book] TS - Tanulmánykötet [Conference Book] PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs PY - 2020 SP - 595 SN - 9789634295839 UR - https://m2.mtmt.hu/api/publication/31789796 ID - 31789796 LA - English DB - MTMT ER - TY - BOOK ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Kovács, Olivér Béla ED - Mező, Emerencia ED - Szabó, Rebeka ED - Guth, Kitti TI - IX. INTERDISZCIPLINÁRIS DOKTORANDUSZ KONFERENCIA 2020 ABSZTRAKTKÖTET. 9th INTERDISCIPLINARY DOCTORAL CONFERENCE 2020 BOOK OF ABSTRACTS TS - 9th INTERDISCIPLINARY DOCTORAL CONFERENCE 2020 BOOK OF ABSTRACTS PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs PY - 2020 SP - 384 SN - 9789634295822 UR - https://m2.mtmt.hu/api/publication/31778530 ID - 31778530 LA - English DB - MTMT ER - TY - CHAP AU - Mező, Emerencia AU - Bufa, Anita AU - Páger, Csilla AU - Kocsis, Béla AU - Kilár, Ferenc AU - Makszin, Lilla ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - Application of Ionic Liquid-Based Column for the Analysis of Fatty Acid Composition in Bacteria and its Lipopolysaccharides by GC-MS T2 - Medical Conference for PhD Students and Experts of Clinical Sciences PB - University of Pécs, Doctoral Student Association CY - Pécs SN - 9789634295440 PY - 2020 SP - 47 UR - https://m2.mtmt.hu/api/publication/31709890 ID - 31709890 LA - English DB - MTMT ER - TY - BOOK ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - Medical Conference for PhD Students and Experts of Clinical Sciences. Book of Abstracts TS - Book of Abstracts PB - University of Pécs, Doctoral Student Association CY - Pécs PY - 2020 SP - 83 SN - 9789634295440 UR - https://m2.mtmt.hu/api/publication/31670822 ID - 31670822 LA - English DB - MTMT ER - TY - JOUR AU - Mező, Emerencia AU - Páger, Csilla AU - Makszin, Lilla AU - Kilár, Ferenc TI - Capillary zone electrophoresis of proteins applying ionic liquids for dynamic coating and as background electrolyte component JF - ELECTROPHORESIS J2 - ELECTROPHORESIS VL - 41 PY - 2020 IS - 24 SP - 2083 EP - 2091 PG - 9 SN - 0173-0835 DO - 10.1002/elps.202000204 UR - https://m2.mtmt.hu/api/publication/31621895 ID - 31621895 AB - The use of ionic liquids in capillary electrophoresis, either as coating material or as components of the background electrolyte needs systematic standardization to set up optimal conditions. Excellent separation of the proteins was achieved using 1-ethyl-3-methylimidazolium tetrafluoroborate ([emim][BF4 ]) or 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4 ]) ionic liquids using the properly made ionic-liquid-water binary mixtures for the experiments. The binary mixture has a distinctly stable and well perceptible low pH, which depends on the concentration of the ionic liquid, and on the preparation time of the mixture. Optimal conditions for the electrophoretic separation were obtained upon a multivariate analysis of the experimental parameters (applied voltage, migration time, concentration and type of the ionic liquid). The standardized condition provides a low electroendosmotic flow towards the anode, which, however, did not hinder the proteins to migrate toward the cathode. The migration of cytochrome c, lysozyme, myoglobin, trypsin and apo-transferrin at a pH around 2, far below the isoelectric points of the proteins, showed RSD values of the migration times less than 7.5% and less than 6.5% when using [emim][BF4 ] or [bmim][BF4 ], respectively, either in run-to-run or day-to-day experiments. The determination of the extent of the EOF is not possible with the commonly used EOF markers, due to interaction with the ionic-liquid constituents. The interaction of the ionic liquids with the proteins influences the migration order in zone electrophoresis. This method has been applied successfully for the analyses of real biological samples, such as proteins from egg whites and human tears. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER -