@article{MTMT:33176667, title = {Effect of Culture Conditions on Fatty Acid Profiles of Bacteria and Lipopolysaccharides of the Genus Pseudomonas—GC-MS Analysis on Ionic Liquid-Based Column}, url = {https://m2.mtmt.hu/api/publication/33176667}, author = {Mező, Emerencia and Balogh-Hartmann, Fruzsina and Madarászné Horváth, Ibolya and Bufa, Anita and Marosvölgyi, Tamás and Kocsis, Béla and Makszin, Lilla}, doi = {10.3390/molecules27206930}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {27}, unique-id = {33176667}, issn = {1420-3049}, abstract = {The profiling of bacterial fatty acids is a well-established technique in identifying and classifying bacteria. Cultivation conditions may affect the biosynthesis, thereby, changing the fatty acid profile in bacteria. The effect of the culture conditions on the fatty acid components of Pseudomonas aeruginosa PAO1, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa polyresistant and Pseudomonas putida all are aligned to the genus Pseudomonas. The fatty acids in the lipopolysaccharides of Pseudomonas aeruginosa PAO1 were also examined. The effects of the cultivation conditions were followed by using agar and blood agar media at the characteristic temperatures, 25 °C, 37 °C and 42 °C, respectively, and an analysis was made during the 1st, 3rd and 5th day following inoculation. In addition to quantitative differences, we also experienced qualitative differences in the fatty acid profiles which detect newly appearing fatty acids, due to changes in environmental factors. The application of ionic liquid-based column unveils new possibilities for the analyses of fatty acids in GC-MS experiments for bacterial fatty acid profiling. The validation results (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) show the high separation efficiency of the ionic liquid-based column in the analyses.}, year = {2022}, eissn = {1420-3049}, orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513; Makszin, Lilla/0000-0002-9764-4763} } @{MTMT:32553805, title = {Környezeti tényezők változásainak hatása baktériumok zsírsavösszetételére}, url = {https://m2.mtmt.hu/api/publication/32553805}, author = {Mező, Emerencia and Bufa, Anita and Madarászné Horváth, Ibolya and Balogh-Hartmann, Fruzsina and Kilár, Ferenc and Kocsis, Béla and Makszin, Lilla}, booktitle = {10th Jubilee Interdisciplinary Doctoral Conference : Book of Abstracts = 10. Jubileumi Interdiszciplináris Doktorandusz Konferencia : Absztraktkötet}, unique-id = {32553805}, year = {2021}, pages = {301-301}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514; Makszin, Lilla/0000-0002-9764-4763} } @{MTMT:32475734, title = {Orvosi szempontból fontos baktériumok zsírsav-összetételének vizsgálata GC-MS módszerrel}, url = {https://m2.mtmt.hu/api/publication/32475734}, author = {Mező, Emerencia and Bufa, Anita and Madarászné Horváth, Ibolya and Balogh-Hartmann, Fruzsina and Kilár, Ferenc and Kocsis, Béla and Makszin, Lilla}, booktitle = {METT25 a Magyar Elválasztástudományi Társaság jubileumi konferenciája}, unique-id = {32475734}, year = {2021}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514; Makszin, Lilla/0000-0002-9764-4763} } @book{MTMT:32024807, title = {Medical Conference for PhD Students and Experts of Clinical Sciences 2021. Book of Abstracts}, url = {https://m2.mtmt.hu/api/publication/32024807}, isbn = {9789634296539}, editor = {Csiszár, Beáta and Hankó, Csilla and Kajos, Luca Fanni and Mező, Emerencia}, publisher = {PTE Doktorandusz Önkormányzat}, unique-id = {32024807}, year = {2021}, orcid-numbers = {Hankó, Csilla/0000-0003-4201-4884; Kajos, Luca Fanni/0000-0002-0574-5787} } @article{MTMT:31933042, title = {The Role of Ionic Liquid Interaction in the Separation of Fatty Acid Methyl Esters—Polyunsaturated Geometric Isomers in GC–MS}, url = {https://m2.mtmt.hu/api/publication/31933042}, author = {Mező, Emerencia and Bufa, Anita and Páger, Csilla and Poór, Viktória and Marosvölgyi, Tamás and Kilár, Ferenc and Makszin, Lilla}, doi = {10.3390/separations8040038}, journal-iso = {SEPARATIONS}, journal = {SEPARATIONS}, volume = {8}, unique-id = {31933042}, issn = {2297-8739}, abstract = {Knowledge of the type and level of saturated and unsaturated fatty acids in food and clinical matrices is of practical importance, but the wide variety of fatty acids makes analyses very complex. The discrimination of the geometric isomers of fatty acid needs proper and effective separation conditions. The efficiency of three different stationary phases was evaluated by GC–MS methods in the separation of fatty acids in their methyl ester forms. Significant differences were observed in the efficiencies of polysiloxane-based (non-polar HP-5MS and medium/high polarity DB-225MS) and ionic liquid-based (SLB-IL111) columns. Baseline separation of the geometric isomers of linoleic acid methyl ester was obtained by the extremely polar SLB-IL111 column, showing a preference over the other two columns. The optimization of the experimental conditions (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) showed the separation power of the ionic liquid interaction in the analyses by using short (25–30 m long) columns. By deducting the general principles of the interaction, predictions can be made for the separation of other isomers. The results facilitate the precise identification of various types of fatty acids in real samples for nutritional information.}, keywords = {VALIDATION; GC-MS; Interaction; Fatty acid methyl ester; geometric isomers; ionic liquid phase; trans fats}, year = {2021}, eissn = {2297-8739}, orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513; Kilár, Ferenc/0000-0001-5737-5514; Makszin, Lilla/0000-0002-9764-4763} } @book{MTMT:31789796, title = {IX. Interdiszciplináris Doktorandusz Konferencia 2020 [9th Interdisciplinary Doctoral Conference 2020]. Tanulmánykötet [Conference Book]}, url = {https://m2.mtmt.hu/api/publication/31789796}, isbn = {9789634295839}, editor = {Csiszár, Beáta and Hankó, Csilla and Kajos, Luca Fanni and Mező, Emerencia}, publisher = {Doctoral Student Association of the University of Pécs}, unique-id = {31789796}, year = {2020}, orcid-numbers = {Hankó, Csilla/0000-0003-4201-4884; Kajos, Luca Fanni/0000-0002-0574-5787} } @book{MTMT:31778530, title = {IX. INTERDISZCIPLINÁRIS DOKTORANDUSZ KONFERENCIA 2020 ABSZTRAKTKÖTET. 9th INTERDISCIPLINARY DOCTORAL CONFERENCE 2020 BOOK OF ABSTRACTS}, url = {https://m2.mtmt.hu/api/publication/31778530}, isbn = {9789634295822}, editor = {Csiszár, Beáta and Hankó, Csilla and Kajos, Luca Fanni and Kovács, Olivér Béla and Mező, Emerencia and Szabó, Rebeka and Guth, Kitti}, publisher = {Doctoral Student Association of the University of Pécs}, unique-id = {31778530}, year = {2020}, orcid-numbers = {Hankó, Csilla/0000-0003-4201-4884; Kajos, Luca Fanni/0000-0002-0574-5787; Kovács, Olivér Béla/0000-0002-8161-7015} } @{MTMT:31709890, title = {Application of Ionic Liquid-Based Column for the Analysis of Fatty Acid Composition in Bacteria and its Lipopolysaccharides by GC-MS}, url = {https://m2.mtmt.hu/api/publication/31709890}, author = {Mező, Emerencia and Bufa, Anita and Páger, Csilla and Kocsis, Béla and Kilár, Ferenc and Makszin, Lilla}, booktitle = {Medical Conference for PhD Students and Experts of Clinical Sciences}, unique-id = {31709890}, year = {2020}, pages = {47}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514; Makszin, Lilla/0000-0002-9764-4763} } @book{MTMT:31670822, title = {Medical Conference for PhD Students and Experts of Clinical Sciences. Book of Abstracts}, url = {https://m2.mtmt.hu/api/publication/31670822}, isbn = {9789634295440}, editor = {Csiszár, Beáta and Hankó, Csilla and Kajos, Luca Fanni and Mező, Emerencia}, publisher = {PTE Doktorandusz Önkormányzat}, unique-id = {31670822}, year = {2020}, orcid-numbers = {Kajos, Luca Fanni/0000-0002-0574-5787} } @article{MTMT:31621895, title = {Capillary zone electrophoresis of proteins applying ionic liquids for dynamic coating and as background electrolyte component}, url = {https://m2.mtmt.hu/api/publication/31621895}, author = {Mező, Emerencia and Páger, Csilla and Makszin, Lilla and Kilár, Ferenc}, doi = {10.1002/elps.202000204}, journal-iso = {ELECTROPHORESIS}, journal = {ELECTROPHORESIS}, volume = {41}, unique-id = {31621895}, issn = {0173-0835}, abstract = {The use of ionic liquids in capillary electrophoresis, either as coating material or as components of the background electrolyte needs systematic standardization to set up optimal conditions. Excellent separation of the proteins was achieved using 1-ethyl-3-methylimidazolium tetrafluoroborate ([emim][BF4 ]) or 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4 ]) ionic liquids using the properly made ionic-liquid-water binary mixtures for the experiments. The binary mixture has a distinctly stable and well perceptible low pH, which depends on the concentration of the ionic liquid, and on the preparation time of the mixture. Optimal conditions for the electrophoretic separation were obtained upon a multivariate analysis of the experimental parameters (applied voltage, migration time, concentration and type of the ionic liquid). The standardized condition provides a low electroendosmotic flow towards the anode, which, however, did not hinder the proteins to migrate toward the cathode. The migration of cytochrome c, lysozyme, myoglobin, trypsin and apo-transferrin at a pH around 2, far below the isoelectric points of the proteins, showed RSD values of the migration times less than 7.5% and less than 6.5% when using [emim][BF4 ] or [bmim][BF4 ], respectively, either in run-to-run or day-to-day experiments. The determination of the extent of the EOF is not possible with the commonly used EOF markers, due to interaction with the ionic-liquid constituents. The interaction of the ionic liquids with the proteins influences the migration order in zone electrophoresis. This method has been applied successfully for the analyses of real biological samples, such as proteins from egg whites and human tears. This article is protected by copyright. All rights reserved.}, keywords = {CAPILLARY ZONE ELECTROPHORESIS; Multivariate statistical analysis; dynamic coating; Imidazolium-based ionic liquids; protein separation, chemical interaction}, year = {2020}, eissn = {1522-2683}, pages = {2083-2091}, orcid-numbers = {Makszin, Lilla/0000-0002-9764-4763; Kilár, Ferenc/0000-0001-5737-5514} }