@article{MTMT:34722559, title = {Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants}, url = {https://m2.mtmt.hu/api/publication/34722559}, author = {Tóth, Balázs István and Bahar, Bazeli and Annelies, Janssens and Lisztes, Erika and Racskó, Márk and Kelemen, Balázs and Herczeg, Mihály and Nagy, Tamás Milán and E Kövér, Katalin and Argha, Mitra and Attila, Borics and Bíró, Tamás and Thomas, Voets}, journal-iso = {ELIFE}, journal = {ELIFE}, unique-id = {34722559}, issn = {2050-084X}, year = {2024}, eissn = {2050-084X}, orcid-numbers = {Herczeg, Mihály/0000-0002-7938-9789} } @article{MTMT:33693788, title = {Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis}, url = {https://m2.mtmt.hu/api/publication/33693788}, author = {Gönczi, Mónika and Teixeira, João M. C. and Barrera-Vilarmau, Susana and Mediani, Laura and Antoniani, Francesco and Nagy, Tamás Milán and Fehér, Krisztina and Ráduly, Zsolt and Ambrus, Viktor Attila and Tőzsér, József and Barta, Endre and E Kövér, Katalin and Csernoch, László and Carra, Serena and Fuxreiter, Mónika}, doi = {10.1038/s41467-023-37017-7}, journal-iso = {NAT COMMUN}, journal = {NATURE COMMUNICATIONS}, volume = {14}, unique-id = {33693788}, issn = {2041-1723}, abstract = {During muscle cell differentiation, the alternatively spliced, acidic β-domain potentiates transcription of Myocyte-specific Enhancer Factor 2 (Mef2D). Sequence analysis by the FuzDrop method indicates that the β-domain can serve as an interaction element for Mef2D higher-order assembly. In accord, we observed Mef2D mobile nuclear condensates in C2C12 cells, similar to those formed through liquid-liquid phase separation. In addition, we found Mef2D solid-like aggregates in the cytosol, the presence of which correlated with higher transcriptional activity. In parallel, we observed a progress in the early phase of myotube development, and higher MyoD and desmin expression. In accord with our predictions, the formation of aggregates was promoted by rigid β-domain variants, as well as by a disordered β-domain variant, capable of switching between liquid-like and solid-like higher-order states. Along these lines, NMR and molecular dynamics simulations corroborated that the β-domain can sample both ordered and disordered interactions leading to compact and extended conformations. These results suggest that β-domain fine-tunes Mef2D higher-order assembly to the cellular context, which provides a platform for myogenic regulatory factors and the transcriptional apparatus during the developmental process.}, keywords = {CELL BIOLOGY; Structural biology; Computational biology and bioinformatics}, year = {2023}, eissn = {2041-1723}, orcid-numbers = {Barta, Endre/0000-0002-6753-0714; Carra, Serena/0000-0003-0939-0140} } @mastersthesis{MTMT:33209861, title = {Understanding structure and dynamics with advanced NMR and in silico methods}, url = {https://m2.mtmt.hu/api/publication/33209861}, author = {Nagy, Tamás Milán}, doi = {10.13140/RG.2.2.18393.80482}, unique-id = {33209861}, abstract = {One of the most powerful tools for studying proteins at molecular level is Nuclear Magnetic Resonance (NMR) spectroscopy. Throughout my undergraduate years, the first encounters with this technique were structural studies carried out on cyclopeptides probing the microenvironment of disulfide bonds. In parallel, I was introduced to intrinsically disordered proteins (IDP) in the form of mini proteins modeling the β-domain of the MEF2D transcription factor. With the hope for establishing a relationship between protein dynamics and function, these studies evolved over the PhD years to better understand the unexpected behavior of the MEF2D protein mutants. NMR relaxation, diffusion measurements, and chemical shift-based calculations were complemented by computational studies (molecular dynamics (MD) simulations). Nanoscale Differential Scanning Fluorometry (NanoDSF) measurements of our collaborators confirmed the oligomerization of one of the peptides, which process might be also essential to understand the biological function of these complex molecules. I have also participated in the development of NMR methods resulting in reduced measurement times and high-quality spectral data with enriched information. To this end, different experiments were combined in NOAH-type single and double BANGO NMR sequences. In addition, exceptionally rapid measurements without relaxation delays were achieved in the NO Relaxation Delay (NORD) spectroscopy. In minutes, complete correlation maps were obtained for small and medium-sized molecules, allowing their quick and unambiguous resonance assignment.}, year = {2022} } @article{MTMT:33085154, title = {Preparation of 3- O -, 5- O - and N -palmitoyl derivatives of fumonisin B 1 toxin and their characterisation with NMR and LC-HRMS methods}, url = {https://m2.mtmt.hu/api/publication/33085154}, author = {Angeli, Cserne and Nagy, Tamás Milán and Horváth, Levente and Varga, Mónika and Szekeres, András and Tóth, Gábor and Janáky, Tamás and Szolomájer, János and Kovács, Melinda and E Kövér, Katalin and Bartók, Tibor}, doi = {10.1080/19440049.2022.2116112}, journal-iso = {FOOD ADDIT CONTAM A}, journal = {FOOD ADDITIVES AND CONTAMINANTS PART A - CHEMISTRY ANALYSIS CONTROL EXPOSURE AND RISK ASSESSMENT}, volume = {39}, unique-id = {33085154}, issn = {1944-0049}, abstract = {We have previously published six esterified O-acyl (EFB1) and three N-acyl fumonisin B-1 derivatives extracted from rice cultures inoculated with Fusarium verticillioides, amongst these the identification of N-palmitoyl-FB1 has been clearly established in a spiking experiment. At that time, it was assumed that as in the case of O-acyl-FB1 derivatives, linoleic-, oleic- or palmitic acid esterify through the OH group on the 3C or 5C atom of the carbon chain of the fumonisins. In our most recent experiments, we have synthetically acylated the FB1 toxin and subsequently purified 3-O-palmitoyl- and 5-O-palmitoyl-FB1 toxins in addition to the N-palmitoyl-FB1 toxin. They were identified and characterised using H-1 and C-13 NMR as well as LC-HRMS. Our aim was the identification of the previously detected O-acyl-FB1 derivatives over the course of a spiking experiment, which were obtained through the solid-phase fermentation of Fusarium verticillioides. By spiking the three synthesized and identified components one-by-one into the fungal culture extract and analysing these cultures using LC-MS, it was clearly demonstrated that the F. verticillioides strain produced both the 5-O-palmitoyl-FB1 and N-palmitoyl-FB1 toxins, but did not produce 3-O-palmitoyl-FB1. Thus, it is highly probable that the components thought to be 3-O-acyl-(linoleoyl-, oleoyl-, palmitoyl-) FB1 derivatives in our previous communication are presumably 10-O-acyl-FB1 derivatives. Since these acylated FB1 derivatives can occur naturally in e.g. maize, the use of these synthesized components as reference materials is of great importance in order to obtain accurate qualitative and quantitative data on the occurrence of acylated fumonisins in different matrices including maize based feed samples. The production of these substances has also made it possible to test their toxicity in cell culture and small animal experiments.}, keywords = {Fusarium; MYCOTOXIN; Fumonisin B1; acylated fumonisin; O-acyl-FB1; N-acyl-FB1; hidden fumonisin}, year = {2022}, eissn = {1944-0057}, pages = {1759-1771}, orcid-numbers = {Szekeres, András/0000-0003-1651-4623; Tóth, Gábor/0000-0002-3604-4385; Janáky, Tamás/0000-0002-6466-8283; Szolomájer, János/0000-0003-1458-6156; Kovács, Melinda/0000-0001-5988-3934} } @article{MTMT:32607584, title = {Synergy and sensitivity-balance in concatenating experiments in NO Relaxation Delay NMR (NORD)}, url = {https://m2.mtmt.hu/api/publication/32607584}, author = {Timári, István and Nagy, Tamás Milán and E Kövér, Katalin and Sørensen, Ole W.}, doi = {10.1039/D1CC06663C}, journal-iso = {CHEM COMMUN}, journal = {CHEMICAL COMMUNICATIONS}, volume = {58}, unique-id = {32607584}, issn = {1359-7345}, abstract = {The NMR experiment design strategy of NO Relaxation Delay (NORD), introduced mostly as an idealized theoretical approach, is extended and put to practical use by considering synergy and sensitivity-balance in concatenation of experiments. It is illustrated by a novel experiment, NORD {HMBC}–{HSQC}–{TOCSY}, where magnetization of non-13C attached protons effectively is channeled from the TOCSY spectrum toward primarily the least sensitive spectrum of HMBC. The experiment is expected to find its place as a full-package NMR method for metabolomics, carbohydrates, peptides and small-molecules in general.}, year = {2022}, eissn = {1364-548X}, pages = {2516-2519} } @article{MTMT:32584830, title = {The First Dimeric Derivatives of the Glycopeptide Antibiotic Teicoplanin}, url = {https://m2.mtmt.hu/api/publication/32584830}, author = {Bakai-Bereczki, Ilona and Szűcs, Zsolt and Batta, Gyula and Nagy, Tamás Milán and Ostorházi, Eszter and E Kövér, Katalin and Borbás, Anikó and Herczegh, Pál}, doi = {10.3390/ph15010077}, journal-iso = {PHARMACEUTICALS-BASE}, journal = {PHARMACEUTICALS}, volume = {15}, unique-id = {32584830}, abstract = {Various dimeric derivatives of the glycopeptide antibiotic teicoplanin were prepared with the aim of increasing the activity of the parent compound against glycopeptide-resistant bacteria, primarily vancomycin-resistant enterococci. Starting from teicoplanin, four covalent dimers were prepared in two orientations, using an α,!-bis-isothiocyanate linker. Formation of a dimeric cobalt coordination complex of an N-terminal L-histidyl derivative of teicoplanin pseudoaglycone has been detected and its antibacterial activity evaluated. The Co(III)-induced dimerization of the histidyl derivative was demonstrated by DOSY experiments. Both the covalent and the complex dimeric derivatives showed high activity against VanA teicoplanin-resistant enterococci, but their activity against other tested bacterial strains did not exceed that of the monomeric compounds.}, keywords = {synthesis; antibacterial activity; DIMER; teicoplanin; DOSY NMR; Co(III) complex; pseudoaglycone}, year = {2022}, eissn = {1424-8247}, orcid-numbers = {Bakai-Bereczki, Ilona/0000-0003-4601-7257; Batta, Gyula/0000-0002-0442-1828; Nagy, Tamás Milán/0000-0003-4766-1992; Ostorházi, Eszter/0000-0002-9459-7316; Borbás, Anikó/0000-0001-8462-4547} } @article{MTMT:31938298, title = {NORD: NO Relaxation Delay NMR spectroscopy}, url = {https://m2.mtmt.hu/api/publication/31938298}, author = {Nagy, Tamás Milán and E Kövér, Katalin and Sørensen, Ole W.}, doi = {10.1002/anie.202102487}, journal-iso = {ANGEW CHEM INT EDIT}, journal = {ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, volume = {60}, unique-id = {31938298}, issn = {1433-7851}, abstract = {The novel concept of NORD (NO relaxation delay) NMR spectroscopy is introduced. The idea is to design concatenated experiments in a way that the magnetization used in the first relaxes toward equilibrium during the second and vice versa, thus saving instrument time. Applications include complete well-resolved 1H-1H and 1H-13C one-bond and long-range correlation maps of an 80 mM solution of a trisaccharide recorded in less than two minutes and hydrocortisone with extensive spectral overlap.}, keywords = {NMR spectroscopy; Heteronuclear correlation; H2OBC/2BOB; NORD; SEA XLOC/HMBC}, year = {2021}, eissn = {1521-3773}, pages = {13587-13590} } @article{MTMT:31334838, title = {Double and adiabatic BANGO for concatenating two NMR experiments relying on the same pool of magnetization}, url = {https://m2.mtmt.hu/api/publication/31334838}, author = {Nagy, Tamás Milán and E Kövér, Katalin and Sørensen, Ole W.}, doi = {10.1016/j.jmr.2020.106767}, journal-iso = {J MAGN RESON}, journal = {JOURNAL OF MAGNETIC RESONANCE}, volume = {316}, unique-id = {31334838}, issn = {1090-7807}, abstract = {It is shown how the same pool of magnetization can be tapped twice in two different concatenations ofthree experiments into a single pulse sequence with only one relaxation delay. This is accomplished byusing the BANGO pulse sequence element twice for independent rotations of1H magnetization attachedor not attached to13C and it includes a refinement of BANGO with an adiabatic13C inversion pulse result-ing in improved tolerance to a spread in1JCHcoupling constants that translates directly into improvedsensitivity of the modular experiment relying on1H magnetization attached to13C. The two new pulsesequences are SEA XLOC–HMBC–H2OBC/2BOB and SEA XLOC(ZQ)–SEA XLOC(2Q)–H2OBC/2BOB whichboth represent a rapid route to complete heteronuclear one-bond and long-rangeJCHcorrelation mapsfor small molecules, as is demonstrated on ibuprofen and prednisolone.}, year = {2020}, eissn = {1096-0856} } @article{MTMT:30931203, title = {BANGO SEA XLOC/HMBC-H2OBC: complete heteronuclear correlation within minutes from one NMR pulse sequence}, url = {https://m2.mtmt.hu/api/publication/30931203}, author = {Nagy, Tamás Milán and Gyöngyösi, Tamás and E Kövér, Katalin and Sorensen, Ole W.}, doi = {10.1039/c9cc06253j}, journal-iso = {CHEM COMMUN}, journal = {CHEMICAL COMMUNICATIONS}, volume = {55}, unique-id = {30931203}, issn = {1359-7345}, abstract = {Novel NMR experiments, BANGO SEA XLOC-H2OBC or BANGO HMBC-H2OBC, can deliver complete heteronuclear correlation maps on a time scale of minutes for small molecules. By way of example, it is demonstrated that all intra- and inter-residue H-1 and C-13 correlations and assignments of a trisaccharide are obtained in 20 or 5 minutes of instrument time without or with 25% NUS, respectively.}, year = {2019}, eissn = {1364-548X}, pages = {12208-12211}, orcid-numbers = {E Kövér, Katalin/0000-0001-5020-4456} } @CONFERENCE{MTMT:3417841, title = {Boosting the NMR assignment of small to medium-sized molecules with different edited variants of HSQC-CLIP-COSY experiment}, url = {https://m2.mtmt.hu/api/publication/3417841}, author = {Nagy, Tamás Milán and Gyöngyösi, Tamás and Boros, Sándor and Burkhard, Luy and Kövér, Katalin}, booktitle = {ABSTRACT BOOK}, doi = {10.13140/RG.2.2.19714.12489}, unique-id = {3417841}, year = {2018} }