TY - JOUR AU - Sere, Péter AU - Nikolett, Zsigri AU - Raffai, Tímea AU - Furdan, Szabina AU - Győri, Fanni AU - Vincenzo, Crunelli AU - Lőrincz, László Magor TI - Activity of the Lateral Hypothalamus during Genetically Determined Absence Seizures JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 17 PG - 8 SN - 1661-6596 DO - 10.3390/ijms22179466 UR - https://m2.mtmt.hu/api/publication/32167983 ID - 32167983 LA - English DB - MTMT ER - TY - JOUR AU - Iacone, Yasmine AU - Morais, Tatiana P. AU - David, Francois AU - Delicata, Francis AU - Sandle, Joanna AU - Raffai, Tímea AU - Parri, Harri Rheinallt AU - Weisser, Johan Juhl AU - Bundgaard, Christoffer AU - Klewe, Ib Vestergaard AU - Tamás, Gábor AU - Thomsen, Morten Skott AU - Crunelli, Vincenzo AU - Lőrincz, László Magor TI - Systemic administration of ivabradine, a hyperpolarization-activated cyclic nucleotide-gated channel inhibitor, blocks spontaneous absence seizures JF - EPILEPSIA J2 - EPILEPSIA VL - 62 PY - 2021 IS - 7 SP - 1729 EP - 1743 PG - 15 SN - 0013-9580 DO - 10.1111/epi.16926 UR - https://m2.mtmt.hu/api/publication/32037613 ID - 32037613 N1 - Funding Agency and Grant Number: Wellcome TrustWellcome TrustEuropean Commission [91882]; Orszagos Tudomanyos Kutatasi AlapprogramokOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [FK123831, NN125601]; Hungarian Brain Research Program [KTIA_NAP_13-2-2014-0014]; Ministry of Human Capacities, Hungary [20391-3/2018/FEKUSTRAT]; Marie Sklodowska-Curie Actions [H2020-MSCA-ITN-2016-722053]; Ester Floridia Neuroscience Research Foundation; Janos Bolyai FellowshipHungarian Academy of Sciences Funding text: Wellcome Trust, Grant/Award Number: 91882; Orszagos Tudomanyos Kutatasi Alapprogramok, Grant/Award Number: FK123831 and NN125601; Hungarian Brain Research Program, Grant/Award Number: KTIA_NAP_13-2-2014-0014; Ministry of Human Capacities, Hungary, Grant/Award Number: 20391-3/2018/FEKUSTRAT; Marie Sklodowska-Curie Actions, Grant/Award Number: H2020-MSCA-ITN-2016-722053; Ester Floridia Neuroscience Research Foundation; Magor L. Lorincz is a grantte of the Janos Bolyai Fellowship AB - Objective Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are known to be involved in the generation of absence seizures (ASs), and there is evidence that cortical and thalamic HCN channel dysfunctions may have a proabsence role. Many HCN channel blockers are available, but their role in ASs has been investigated only by localized brain injection or in in vitro model systems due to their limited brain availability. Here, we investigated the effect on ASs of orally administered ivabradine (an HCN channel blocker approved for the treatment of heart failure in humans) following injection of the P-glycoprotein inhibitor elacridar, which is known to increase penetration into the brain of drug substrates for this efflux transporter. The action of ivabradine was also tested following in vivo microinjection into the cortical initiation network (CIN) of the somatosensory cortex and in the thalamic ventrobasal nucleus (VB) as well as on cortical and thalamocortical neurons in brain slices. Methods We used electroencephalographic recordings in freely moving Genetic Absence Epilepsy Rats From Strasbourg (GAERSs) to assess the action of oral administration of ivabradine, with and without elacridar, on ASs. Ivabradine was also microinjected into the CIN and VB of GAERSs in vivo and applied to Wistar CIN and GAERS VB slices while recording patch-clamped cortical Layer 5/6 and thalamocortical neurons, respectively. Results Oral administration of ivabradine markedly and dose-dependently reduced ASs. Ivabradine injection into CIN abolished ASs and elicited small-amplitude 4-7-Hz waves (without spikes), whereas in the VB it was less potent. Moreover, ivabradine applied to GAERS VB and Wistar CIN slices selectively decreased HCN channel-dependent properties of cortical Layer 5/6 pyramidal and thalamocortical neurons, respectively. Significance These results provide the first demonstration of the antiabsence action of a systemically administered HCN channel blocker, indicating the potential of this class of drugs as a novel therapeutic avenue for ASs. LA - English DB - MTMT ER - TY - CHAP AU - Csábi, József AU - Raffai, Tímea AU - Hunyadi, Attila AU - Zádor, Ernő ED - Rakonczay, Zoltán ED - Kiss, Lóránd TI - Poststerone increases muscle fibre size partly similar to its metabolically parent compound, 20-hydroxyecdysone T2 - Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project PB - University of Szeged CY - Szeged SN - 9789633067642 PY - 2020 SP - 93 UR - https://m2.mtmt.hu/api/publication/31647881 ID - 31647881 LA - English DB - MTMT ER - TY - THES AU - Raffai, Tímea TI - Influence of chemical compounds and cellautonomous immunity on the replication of sexually transmitted pathogens [Különböző kémiai vegyületek és a sejt-autonóm immunitás hatásai a szexuálisan terjedő kórokozók szaporodására] PB - Szegedi Tudományegyetem (SZTE) PY - 2020 SP - 89 DO - 10.14232/phd.10379 UR - https://m2.mtmt.hu/api/publication/31390118 ID - 31390118 LA - English DB - MTMT ER - TY - JOUR AU - Párduczné Szöllősi, Andrea AU - Raffai, Tímea AU - Bogdanov, Anita AU - Endrész, Valéria AU - Párducz, László AU - Somogyvári, Ferenc AU - Janovák, László AU - Burián, Katalin AU - Virók, Dezső TI - Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels JF - BMC RESEARCH NOTES J2 - BMC RES NOTES VL - 13 PY - 2020 IS - 1 PG - 6 SN - 1756-0500 DO - 10.1186/s13104-020-4918-4 UR - https://m2.mtmt.hu/api/publication/31156856 ID - 31156856 N1 - Department of Health and Social Sciences, Gál Ferenc College, Szent István st. 17-19, Gyula, 5700, Hungary Department of Medical Microbiology and Immunobiology, University of Szeged, Dóm sqr. 10, Szeged, 6720, Hungary Pándy Kálmán County Hospital, Semmelweis st. 1, Gyula, 5700, Hungary Interdisciplinary Excellence Centre, Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich Béla sqr. 1, Szeged, 6720, Hungary Export Date: 24 June 2020 Correspondence Address: Virok, D.P.; Department of Health and Social Sciences, Gál Ferenc College, Szent István st. 17-19, Hungary; email: virok.dezso.peter@med.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Vanic, Zeljka AU - Rukavina, Zora AU - Manner, Suvi AU - Fallarero, Adyary AU - Uzelac, Lidija AU - Kralj, Marijeta AU - Klaric, Daniela Amidzic AU - Bogdanov, Anita AU - Raffai, Tímea AU - Virók, Dezső AU - Filipovic-Grcic, Jelena AU - Skalko-Basnet, Natasa TI - Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections JF - INTERNATIONAL JOURNAL OF NANOMEDICINE J2 - INT J NANOMED VL - 14 PY - 2019 SP - 5957 EP - 5976 PG - 20 SN - 1176-9114 DO - 10.2147/IJN.S211691 UR - https://m2.mtmt.hu/api/publication/30810007 ID - 30810007 N1 - Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, 10000, Croatia Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi and University of Helsinki, Turku, 20520, Finland Division of Pharmaceutical Biosciences, Pharmaceutical Biology, Faculty of Pharmacy, University of Helsinki, Helsinki, 00014, Finland Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, 10000, Croatia Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Drug Transport and Delivery Research Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø the Arctic University of Norway, Tromsø, 5037, Norway Export Date: 3 December 2019 Correspondence Address: Vanić, Ž.; Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Croatia; email: zvanic@pharma.hr AB - Background: Efficient localized cervicovaginal antibacterial therapy, enabling the delivery of antibiotic to the site of action at lower doses while escaping systemic drug effects and reducing the risk of developing microbial resistance, is attracting considerable attention. Liposomes have been shown to allow sustained drug release into vaginal mucosa and improve delivery of antibiotics to bacterial cells and biofilms Azithromycin (AZI), a potent broad-spectrum macrolide antibiotic, has not yet been investigated for localized therapy of cervicovaginal infections, although it is administered orally for the treatment of sexually transmitted diseases. Encapsulation of AZI in liposomes could improve its solubility, antibacterial activity, and allow the prolonged drug release in the cervicovaginal tissue, while avoiding systemic side effects.Purpose: The objective of this study was to develop AZI-liposomes and explore their potentials for treating cervicovaginal infections.Methods: AZI-liposomes that differed in bilayer elasticity/rigidity and surface charge were prepared and evaluated under simulated cervicovaginal conditions to yield optimized liposomes, which were assessed for antibacterial activity against several planktonic and biofilm-forming Escherichia coli strains and intracellular Chlamydia trachomatis, ex vivo AZI vaginal deposition/penetration, and in vitro cytotoxicity toward cervical cells.Results: Negatively charged liposomes with rigid bilayers (CL-3), propylene glycol liposomes (PGL-2) and deformable propylene glycol liposomes (DPGL-2) were efficient against planktonic E. coli ATCC 700928 and K-12. CL-3 was superior for preventing the formation of E. coli ATCC 700928 and K-12 biofilms, with IC50 values (concentrations that inhibit biofilm viability by 50%) up to 8-fold lower than those of the control (free AZI). DPGL-2 was the most promising for eradication of already formed E. coli biofilms and for treating C. trachomatis infections. All AZI-liposomes were biocompatible with cervical cells and improved localization of the drug inside vaginal tissue compared with the control.Conclusion: The performed studies confirm the potentials of AZI-liposomes for localized cervicovaginal therapy. LA - English DB - MTMT ER - TY - CHAP AU - Meštrović, Tomislav AU - Virók, Dezső AU - Ljubin-Sternak, Sunčanica AU - Raffai, Tímea AU - Burián, Katalin AU - Vraneš, Jasmina ED - Brown, Amanda Claire TI - Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth T2 - Chlamydia trachomatis PB - Springer New York CY - New York, New York SN - 9781493996933 T3 - Methods in Molecular Biology, ISSN 1064-3745 ; 2042. PY - 2019 SP - 33 EP - 43 PG - 11 DO - 10.1007/978-1-4939-9694-0_5 UR - https://m2.mtmt.hu/api/publication/30807840 ID - 30807840 LA - English DB - MTMT ER - TY - JOUR AU - Csábi, József AU - Raffai, Tímea AU - Hunyadi, Attila AU - Zádor, Ernő TI - Poststerone increases muscle fibre size partly similar to its metabolically parent compound, 20-hydroxyecdysone JF - FITOTERAPIA J2 - FITOTERAPIA VL - 134 PY - 2019 SP - 459 EP - 464 PG - 6 SN - 0367-326X DO - 10.1016/j.fitote.2019.03.017 UR - https://m2.mtmt.hu/api/publication/30773411 ID - 30773411 N1 - 30611664 másolata. LA - English DB - MTMT ER - TY - JOUR AU - Virók, Dezső AU - Raffai, Tímea AU - Kókai, Dávid AU - Paróczai, Dóra AU - Bogdanov, Anita AU - Veres, Gábor AU - Vécsei, László AU - Póliska, Szilárd AU - Tiszlavicz, László AU - Somogyvári, Ferenc AU - Endrész, Valéria AU - Burián, Katalin TI - Indoleamine 2,3-Dioxygenase Activity in Chlamydia muridarum and Chlamydia pneumoniae Infected Mouse Lung Tissues JF - FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY J2 - FRONT CELL INFECT MI VL - 9 PY - 2019 PG - 12 SN - 2235-2988 DO - 10.3389/fcimb.2019.00192 UR - https://m2.mtmt.hu/api/publication/30735137 ID - 30735137 N1 - The study was supported by the EFOP-3.6.1-16-2016-00008 European Union—Hungary grant and the UNKP-18-3 New National Excellence Program of the Ministry of Human Capacities, Hungary. Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary MTA-SZTE Neuroscience Research Group, Szeged, Hungary Department of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged, Hungary Genomic Medicine and Bioinformatics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Pathology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged, Hungary Export Date: 3 December 2019 Correspondence Address: Virok, D.P.; Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of SzegedHungary; email: virok.dezso.peter@med.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Párduczné Szöllősi, Andrea AU - Párducz, László AU - Raffai, Tímea AU - Virók, Dezső TI - Kereskedelmi forgalomban levő hüvelyi gélek herpesz szimplex vírus-2 ellenes hatásának vizsgálata JF - BULLETIN OF MEDICAL SCIENCES / ORVOSTUDOMÁNYI ÉRTESÍTŐ J2 - ORVOSTUDOMÁNYI ÉRTESÍTŐ VL - 92 PY - 2019 IS - 2. Különszám SP - 38 EP - 39 PG - 2 SN - 1453-0953 UR - https://m2.mtmt.hu/api/publication/30637094 ID - 30637094 LA - Hungarian DB - MTMT ER -