TY - JOUR AU - Kushkevych, Ivan AU - Martínková, Kristýna AU - Mráková, Lenka AU - Giudici, Francesco AU - Baldi, Simone AU - Novak, David AU - Gajdács, Márió AU - Vítězová, Monika AU - Dordevic, Dani AU - Amedei, Amedeo AU - Rittmann, Simon K.-M. R. TI - Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study JF - MICROBIAL CELL J2 - MICROBIAL CELL VL - 11 PY - 2024 IS - 1 SP - 79 EP - 89 PG - 11 SN - 2311-2638 DO - 10.15698/mic2024.03.817 UR - https://m2.mtmt.hu/api/publication/34741193 ID - 34741193 AB - Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition – focusing on sulfate-reducing bacteria (SRB) – in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients’ disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases. LA - English DB - MTMT ER - TY - JOUR AU - Pallós, Péter AU - Gajdács, Márió AU - Zsoldiné Urbán, Edit AU - Szabados, Yvett AU - Szalai, Klaudia AU - Hevesi, Lívia AU - Horváth, Anna AU - Kuklis, Anna AU - Morjaria, Devina AU - Iffat, Wajiha AU - Hetta, Helal F. AU - Piredda, Nicola AU - Donadu, Matthew Gavino TI - Characterization of antibiotic and disinfectant susceptibility in biofilm-forming Acinetobacter baumannii: A focus on environmental isolates JF - EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY J2 - EUR J MICROBIOL IMMU PY - 2024 SN - 2062-509X DO - 10.1556/1886.2024.00014 UR - https://m2.mtmt.hu/api/publication/34721811 ID - 34721811 N1 - Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza Lajos krt. 64-66., Szeged, 6720, Hungary Department of Medical Microbiology and Immunology, University of Pécs Medical School, Szigeti út 12, Pécs, 7624, Hungary Department of Pharmaceutics, Dow College of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Dow University of Health Sciences, OJHA Campus, Karachi, Pakistan Department of Natural Products and Alternative Medicine, Division of Microbiology and Immunology, Faculty of Pharmacy, University of Tabuk, Tabuk, 71491, Saudi Arabia Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt Radiology Unit, Giovanni Paolo II Hospital, ASL Gallura, Olbia, 07026, Italy Hospital Pharmacy, Giovanni Paolo II Hospital, ASL Gallura, Olbia, 07026, Italy Department of Medicine, Surgery and Pharmacy, Scuola di Specializzazione in Farmacia Ospedaliera, University of Sassari, Sassari, 07100, Italy Export Date: 19 April 2024 Correspondence Address: Gajdács, M.; Department of Oral Biology and Experimental Dental Research, Tisza Lajos krt. 64-66., Hungary; email: mariopharma92@gmail.com Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, ÚNKP-23-1-SZTE-146 Funding text 1: A.K. was supported by the New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research Development and Innovation Fund (ÚNKP-23-1-SZTE-146). The authors are thankful for the support of the Study Group for Dental Research Methodology and Health Sciences, University of Szeged. AB - The clinical role of Acinetobacter baumannii has been highlighted in numerous infectious syndromes with a high mortality rate, due to the high prevalence of multidrug-resistant (MDR) isolates. The treatment and eradication of this pathogen is hindered by biofilm-formation, providing protection from noxious environmental factors and antimicrobials. The aim of this study was to assess the antibiotic susceptibility, antiseptic susceptibility and biofilm-forming capacity using phenotypic methods in environmental A. baumannii isolates. One hundred and fourteen ( n = 114) isolates were collected, originating from various environmental sources and geographical regions. Antimicrobial susceptibility testing was carried out using the disk diffusion method, while antiseptic susceptibility was performed using the agar dilution method. Determination of biofilm-forming capacity was carried out using a microtiter-plate based method. Resistance in environmental A. baumannii isolates were highest for ciprofloxacin (64.03%, n = 73), levofloxacin (62.18%, n = 71) and trimethoprim-sulfamethoxazole (61.40%, n = 70), while lowest for colistin (1.75%, n = 2). Efflux pump overexpression was seen in 48.25% of isolates ( n = 55), 49.12% ( n = 56) were classified as MDR. 6.14% ( n = 7), 9.65% ( n = 11), 24.65% ( n = 28) and 59.65% ( n = 68) of isolates were non-biofilm producers, weak, medium, and strong biofilm producers, respectively. No significant differences were observed between non-MDR vs. MDR isolates regarding their distribution of biofilm-producers ( P = 0.655). The MIC ranges for the tested antiseptics were as follows: benzalkonium chloride 16–128 μg mL −1 , chlorhexidine digluconate 4–128 μg mL −1 , formaldehyde 64–256 μg mL −1 and triclosan 2–16 μg mL −1 , respectively. The conscientious use of antiseptics, together with periodic surveillance, is essential to curb the spread of these bacteria, and to maintain current infection prevention capabilities. LA - English DB - MTMT ER - TY - JOUR AU - Gajdács, Márió AU - Matuz, Mária AU - Benkő, Ria AU - Pető, Zoltán AU - Hajdú, Edit TI - Correlation between Stenotrophomonas maltophilia incidence and systemic antibiotic use: A 10-year retrospective, observational study in Hungary JF - EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY J2 - EUR J MICROBIOL IMMU PY - 2024 SN - 2062-509X DO - 10.1556/1886.2024.00022 UR - https://m2.mtmt.hu/api/publication/34721808 ID - 34721808 N1 - Export Date: 12 April 2024 Correspondence Address: Gajdács, M.; Department of Oral Biology and Experimental Dental Research, Tisza Lajos krt. 64-66, Hungary; email: mariopharma92@gmail.com Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding text 1: The authors are thankful for the support of the Study Group for Dental Research Methodology and Health Sciences, University of Szeged. Part of the study was presented at the 31st European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Project no. TKP2021-EGA-32 has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme. AB - Extensive use of carbapenems may lead to selection pressure for Stenotrophomonas maltophilia (SM) in hospital environments. The aim of our study was to assess the possible association between systemic antibiotic use and the incidence of SM. A retrospective, observational study was carried out in a tertiary-care hospital in Hungary, between January 1st 2010 and December 31st 2019. Incidence-density for SM and SM resistant to trimethoprim-sulfamethoxazole (SXT) was standardized for 1000 patient-days, while systemic antibiotic use was expressed as defined daily doses (DDDs) per 100 patient-days. Mean incidence density for SM infections was 0.42/1000 patient-days; 11.08% were were resistant to SXT, the mean incidence density for SXT-resistant SM was 0.047/1000 patient-days. Consumption rate for colistin, glycopeptides and carbapenems increased by 258.82, 278.94 and 372.72% from 2010 to 2019, respectively. Strong and significant positive correlations were observed with the consumption of carbapenems (r: 0.8759; P < 0.001 and r: 0.8968; P < 0.001), SXT (r: 0.7552; P = 0.011 and r: 0.7004; P = 0.024), and glycopeptides (r: 0.7542; P = 0.012 and r: 0.8138; P < 0.001) with SM and SXT-resistant SM incidence-density/1000 patient-days, respectively. Implementation of institutional carbapenem-sparing strategies are critical in preserving these life-saving drugs, and may affect the microbial spectrum of infections in clinical settings. LA - English DB - MTMT ER - TY - JOUR AU - Gajdács, Márió AU - Jamshed, Shazia TI - Editorial: Knowledge, attitude and practices of the public and healthcare-professionals towards sustainable use of antimicrobials: the intersection of pharmacology and social medicine JF - Frontiers in Antibiotics J2 - Front. Antibiot. VL - 3 PY - 2024 PG - 3 SN - 2813-2467 DO - 10.3389/frabi.2024.1374463 UR - https://m2.mtmt.hu/api/publication/34654218 ID - 34654218 LA - English DB - MTMT ER - TY - CONF AU - Pallós, Péter Márk AU - Szikora, Fruzsina AU - Balogh, Georgina AU - Gajdács, Márió TI - A probiotikumokra és bél-mikrobiótára vonatkozó internetes keresések Google TrendsTM-alapú infodemiológiai vizsgálata: hazai viszonyok és nemzetkozi kitekintés T2 - MHT XLVIII. Vándorgyűlés - Program és összefoglalók PY - 2023 SP - 45 EP - 45 PG - 1 UR - https://m2.mtmt.hu/api/publication/34501884 ID - 34501884 AB - Magyar Higiénikusok Társasága XLVIII. Vándorgyűlés 2023. szeptember 5-6., Szentendre LA - Hungarian DB - MTMT ER - TY - CONF AU - Gajdács, Márió AU - Márta, Adrienn AU - Burián, Katalin AU - Benkő, Ria AU - Paulik, Edit AU - Szabó, Andrea TI - Hogyan befolyásolta a COVID-19 világjárvány a gyógyszerészhallgatók ismereteit és attitűdjét a fertőző betegségekkel és antimikrobiális rezisztenciával kapcsolatban? Egy összehasonlító, keresztmetszeti vizsgálat eredményei T2 - MHT XLVIII. Vándorgyűlés - Program és összefoglalók PY - 2023 SP - 29 EP - 29 PG - 1 UR - https://m2.mtmt.hu/api/publication/34501879 ID - 34501879 AB - Magyar Higiénikusok Társasága XLVIII. Vándorgyűlés 2023. szeptember 5-6., Szentendre LA - Hungarian DB - MTMT ER - TY - CONF AU - Gajdács, Márió AU - Balog, Péter AU - Kívés, Zsuzsanna AU - Háznagyné Radnai, Erzsébet AU - Burián, Katalin AU - Benkő, Ria AU - Paulik, Edit AU - Szabó, Andrea TI - A hazai gyógyszerészek fertőző betegségekkel és antimikrobiális rezisztenciával kapcsolatos attitűdje és ismeretei: kvantitatív, keresztmetszeti vizsgálat T2 - MHT XLVIII. Vándorgyűlés - Program és összefoglalók PY - 2023 SP - 28 EP - 28 PG - 1 UR - https://m2.mtmt.hu/api/publication/34501876 ID - 34501876 AB - Magyar Higiénikusok Társasága XLVIII. Vándorgyűlés 2023. szeptember 5-6., Szentendre LA - Hungarian DB - MTMT ER - TY - CONF AU - Pallós, Péter Márk AU - Kívés, Zsuzsanna AU - Gajdács, Márió TI - A hazai antibiotikumokra és antibiotikum-rezisztenciára vonatkozó, 2010-2020 közötti internetes keresések kvalitatív és kvantitatív elemzése: mit javasol „Dr. Google”? T2 - XV. Fiatal Higiénikusok Fóruma: Program és összefoglalók PB - Magyar Higiénikusok Társasága PY - 2023 SP - 25 EP - 25 PG - 1 UR - https://m2.mtmt.hu/api/publication/34500725 ID - 34500725 LA - Hungarian DB - MTMT ER - TY - CONF AU - Gajdács, Márió AU - Benkő, Ria AU - Matuz, Mária AU - Bodó, Gabriella AU - Pető, Zoltán AU - Hajdú, Edit TI - Stenotrophomonas maltophilia epidemiológiája és az antibiotikum-fogyasztás közötti összefüggések: 10-éves korrelációs vizsgálat T2 - XV. Fiatal Higiénikusok Fóruma: Program és összefoglalók PB - Magyar Higiénikusok Társasága PY - 2023 SP - 12 EP - 12 PG - 1 UR - https://m2.mtmt.hu/api/publication/34500723 ID - 34500723 LA - Hungarian DB - MTMT ER - TY - CONF AU - Gajdács, Márió AU - Kuklis, Anna AU - Balogh, Georgina AU - Kárász, Sára AU - Háznagyné Radnai, Erzsébet TI - Gyógyszerészek kiégési szintje és azt befolyásoló tényezők a SARS-CoV-2 pandémia első hulláma alatt T2 - XV. Fiatal Higiénikusok Fóruma: Program és összefoglalók PB - Magyar Higiénikusok Társasága PY - 2023 SP - 11 EP - 11 PG - 1 UR - https://m2.mtmt.hu/api/publication/34500722 ID - 34500722 LA - Hungarian DB - MTMT ER -