TY - JOUR AU - Sipos, Bence AU - Bella, Zsolt AU - Gróf, Ilona AU - Veszelka, Szilvia AU - Deli, Mária Anna AU - Szűcs, Kálmán Ferenc AU - Sztojkov-Ivanov, Anita AU - Ducza, Eszter AU - Gáspár, Róbert AU - Kecskeméti, Gábor AU - Janáky, Tamás AU - Volk, Balázs AU - Budai-Szűcs, Mária AU - Ambrus, Rita AU - Révész, Piroska AU - Pannonhalminé Csóka, Ildikó AU - Katona, Gábor TI - Soluplus® promotes efficient transport of meloxicam to the central nervous system via nasal administration JF - INTERNATIONAL JOURNAL OF PHARMACEUTICS J2 - INT J PHARM VL - 632 PY - 2023 PG - 11 SN - 0378-5173 DO - 10.1016/j.ijpharm.2023.122594 UR - https://m2.mtmt.hu/api/publication/33547706 ID - 33547706 LA - English DB - MTMT ER - TY - JOUR AU - Kata, Diána AU - Gróf, Ilona AU - Hoyk, Zsófia AU - Ducza, Eszter AU - Deli, Mária Anna AU - Zupkó, István AU - Földesi, Imre TI - Immunofluorescent Evidence for Nuclear Localization of Aromatase in Astrocytes in the Rat Central Nervous System JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 16 PG - 21 SN - 1661-6596 DO - 10.3390/ijms23168946 UR - https://m2.mtmt.hu/api/publication/33079552 ID - 33079552 N1 - Export Date: 18 April 2023 AB - Estrogens regulate a variety of neuroendocrine, reproductive and also non-reproductive brain functions. Estradiol biosynthesis in the central nervous system (CNS) is catalyzed by the enzyme aromatase, which is expressed in several brain regions by neurons, astrocytes and microglia. In this study, we performed a complex fluorescent immunocytochemical analysis which revealed that aromatase is colocalized with the nuclear stain in glial fibrillary acidic protein (GFAP) positive astrocytes in cell cultures. Confocal immunofluorescent Z-stack scanning analysis confirmed the colocalization of aromatase with the nuclear DAPI signal. Nuclear aromatase was also detectable in the S100 beta positive astrocyte subpopulation. When the nuclear aromatase signal was present, estrogen receptor alpha was also abundant in the nucleus. Immunostaining of frozen brain tissue sections showed that the nuclear colocalization of the enzyme in GFAP-positive astrocytes is also detectable in the adult rat brain. CD11b/c labelled microglial cells express aromatase, but the immunopositive signal was distributed only in the cytoplasm both in the ramified and amoeboid microglial forms. Immunostaining of rat ovarian tissue sections and human granulosa cells revealed that aromatase was present only in the cytoplasm. This novel observation suggests a new unique mechanism in astrocytes that may regulate certain CNS functions via estradiol production. LA - English DB - MTMT ER - TY - THES AU - Gróf, Ilona TI - Tenyészetes epitélsejt modellek alkalmazása biológiailag aktív peptidek és adjuváns terápiás szerek hatásának vizsgálatára [Application of epithelial cell culture models to study the effects of biologically active peptides and adjuvant therapeutic agents] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 70 DO - 10.14232/phd.10864 UR - https://m2.mtmt.hu/api/publication/32846886 ID - 32846886 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hetényi, Anasztázia AU - Imre, Norbert AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Fehérje méretű molekulák humán sejtekbe juttatása lipid-raft mediált endocitózissal JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 45 PY - 2021 IS - 4 SP - 67 EP - 83 PG - 17 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32570862 ID - 32570862 N1 - Nincs jelölve levelező szerzőség a közleményen. (BÉ SZTE admin5) LA - Hungarian DB - MTMT ER - TY - JOUR AU - Akel, Hussein AU - Pannonhalminé Csóka, Ildikó AU - Ambrus, Rita AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Mészáros, Mária AU - Szecskó, Anikó AU - Kozma, Gábor AU - Veszelka, Szilvia AU - Deli, Mária Anna AU - Kónya, Zoltán AU - Katona, Gábor TI - In Vitro Comparative Study of Solid Lipid and PLGA Nanoparticles Designed to Facilitate Nose-to-Brain Delivery of Insulin JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 24 PG - 22 SN - 1661-6596 DO - 10.3390/ijms222413258 UR - https://m2.mtmt.hu/api/publication/32531953 ID - 32531953 N1 - Funding Agency and Grant Number: Ministry of Human Capacities, Hungary [TKP-2020]; National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP2.3.2-15-2016-00060] Funding text: The publication was founded by the Ministry of Human Capacities, Hungary (Grant TKP-2020), and by the National Research, Development and Innovation Office, Hungary (GINOP2.3.2-15-2016-00060) projects. LA - English DB - MTMT ER - TY - JOUR AU - Ruksakiet, Kasidid AU - Stercz, Balázs AU - Tóth, Gergő AU - Pongsiri, Jaikumpun AU - Gróf, Ilona AU - Tengölics, Roland AU - Lohinai, Zsolt AU - Horváth, Péter AU - Deli, Mária Anna AU - Steward, Martin Charles AU - Dobay, Orsolya AU - Zsembery, Ákos TI - Bicarbonate Evokes Reciprocal Changes in Intracellular Cyclic di-GMP and Cyclic AMP Levels in Pseudomonas aeruginosa JF - BIOLOGY-BASEL J2 - BIOLOGY-BASEL VL - 10 PY - 2021 IS - 6 PG - 12 SN - 2079-7737 DO - 10.3390/biology10060519 UR - https://m2.mtmt.hu/api/publication/32067156 ID - 32067156 N1 - Export Date: 15 September 2022 LA - English DB - MTMT ER - TY - JOUR AU - Katona, Gábor AU - Sipos, Bence AU - Budai-Szűcs, Mária AU - Balogh, György Tibor AU - Veszelka, Szilvia AU - Gróf, Ilona AU - Deli, Mária Anna AU - Volk, Balázs AU - Révész, Piroska AU - Pannonhalminé Csóka, Ildikó TI - Development of In Situ Gelling Meloxicam-Human Serum Albumin Nanoparticle Formulation for Nose-to-Brain Application JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 13 PY - 2021 IS - 5 PG - 22 SN - 1999-4923 DO - 10.3390/pharmaceutics13050646 UR - https://m2.mtmt.hu/api/publication/31995751 ID - 31995751 N1 - A kutatómunka az Innovációs és Technológiai Minisztérium ÚNKP-20-4-SZTE-327 kódszámú Új Nemzeti Kiválóság Programjának a Nemzeti Kutatási, Fejlesztési és Innovációs Alapból finanszírozott szakmai támogatásával készült. AB - The aim of this study was to develop an intranasal in situ thermo-gelling meloxicam-human serum albumin (MEL-HSA) nanoparticulate formulation applying poloxamer 407 (P407), which can be administered in liquid state into the nostril, and to increase the resistance of the formulation against mucociliary clearance by sol-gel transition on the nasal mucosa, as well as to improve drug absorption. Nanoparticle characterization showed that formulations containing 12-15% w/w P407 met the requirements of intranasal administration. The Z-average (in the range of 180-304 nm), the narrow polydispersity index (PdI, from 0.193 to 0.328), the zeta potential (between -9.4 and -7.0 mV) and the hypotonic osmolality (200-278 mOsmol/L) of MEL-HSA nanoparticles predict enhanced drug absorption through the nasal mucosa. Based on the rheological, muco-adhesion, drug release and permeability studies, the 14% w/w P407 containing formulation (MEL-HSA-P14%) was considered as the optimized formulation, which allows enhanced permeability of MEL through blood-brain barrier-specific lipid fraction. Cell line studies showed no cell damage after 1-h treatment with MEL-HSA-P14% on RPMI 2650 human endothelial cells' moreover, enhanced permeation (four-fold) of MEL from MEL-HSA-P14% was observed in comparison to pure MEL. Overall, MEL-HSA-P14% can be promising for overcoming the challenges of nasal drug delivery. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Tivadar AU - Bocsik, Alexandra AU - Jurišić Dukovski, Bisera AU - Gróf, Ilona AU - Lovrić, Jasmina AU - Pannonhalminé Csóka, Ildikó AU - Deli, Mária Anna AU - Aigner, Zoltán TI - New Approach in Ocular Drug Delivery. In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations TS - In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations JF - DRUG DESIGN DEVELOPMENT AND THERAPY J2 - DRUG DES DEV THER VL - 15 PY - 2021 SP - 351 EP - 360 PG - 10 SN - 1177-8881 DO - 10.2147/DDDT.S264745 UR - https://m2.mtmt.hu/api/publication/31890902 ID - 31890902 AB - Background: Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye. Antimicrobial stability of topical ophthalmic formulations is especially important. According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required. In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects. Methods: The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model. The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex. Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride. Results: As the results show, benzalkonium-chloride-containing samples were toxic on HCE-T cells. The biopolymer caused no cell damage after the treatment. This was confirmed by immunohistochemistry assay. The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation. According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability. Conclusion: Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration. It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Tivadar AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Bisera, Jurisic Dukowski AU - Jasmina, Lovric AU - Deli, Mária Anna AU - Aigner, Zoltán TI - Investigation of the permeability and cytotoxicity in novel topical ophthalmic formulations using in vitro and ex vivo models JF - ACTA PHARMACEUTICA HUNGARICA J2 - ACTA PHARM HUNG VL - 90 PY - 2020 IS - 2-3 SP - 86 EP - 86 PG - 1 SN - 0001-6659 UR - https://m2.mtmt.hu/api/publication/31978759 ID - 31978759 LA - English DB - MTMT ER - TY - JOUR AU - L. Kiss, Eszter AU - Berkó, Szilvia AU - Gácsi, Attila AU - Kovács, Anita AU - Katona, Gábor AU - Soós, Judit AU - Csányi, Erzsébet AU - Gróf, Ilona AU - Harazin, András AU - Deli, Mária Anna AU - Balogh, György Tibor AU - Budai-Szűcs, Mária TI - Development and Characterization of Potential Ocular Mucoadhesive Nano Lipid Carriers Using Full Factorial Design JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 12 PY - 2020 IS - 7 PG - 23 SN - 1999-4923 DO - 10.3390/pharmaceutics12070682 UR - https://m2.mtmt.hu/api/publication/31385933 ID - 31385933 N1 - Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi Fasor 10-11, Szeged, H-6720, Hungary Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Doctoral School of Biology, University of Szeged, Dugonics tér 13, Szeged, H-6720, Hungary Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Műegyetem rakpart 3, Budapest, 1111, Hungary Cited By :11 Export Date: 1 March 2024 Correspondence Address: Budai-Szűcs, M.; Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Hungary; email: maria.szucs@pharm.u-szeged.hu Chemicals/CAS: cremophor, 39279-69-1, 51142-51-9; dexamethasone, 50-02-2; glycerol behenate, 18641-57-1; hydrogenated castor oil, 8001-78-3; hydroxypropylmethylcellulose, 9004-65-3; macrogol, 25322-68-3; miglyol, 37332-31-3, 77466-09-2, 77944-80-0, 97708-73-1; occludin, 176304-61-3; uvomorulin, 112956-45-3 LA - English DB - MTMT ER -