@article{MTMT:33728546, title = {Virulence Characteristics and Molecular Typing of Carbapenem-Resistant ST15 Klebsiella pneumoniae Clinical Isolates, Possessing the K24 Capsular Type}, url = {https://m2.mtmt.hu/api/publication/33728546}, author = {Horváth, Marianna and Kovács, Tamás and Kun, József and Gyenesei, Attila and Damjanova, Ivelina and Tigyi, Zoltán and Schneider, György}, doi = {10.3390/antibiotics12030479}, journal-iso = {ANTIBIOTICS-BASEL}, journal = {ANTIBIOTICS}, volume = {12}, unique-id = {33728546}, abstract = {Klebsiella pneumoniae is an opportunistic pathogen that frequently causes nosocomial and community-acquired (CA) infections. Until now, a limited number of studies has been focused on the analyses of changes affecting the virulence attributes. Genotypic and phenotypic methods were used to characterise the 39 clinical K. pneumoniae isolates; all belonged to the pan-drug resistant, widespread clone ST 15 and expressed the K24 capsule. PFGE has revealed that the isolates could be divided into three distinct genomic clusters. All isolates possessed allS and uge genes, known to contribute to the virulence of K. pneumoniae and 10.25% of the isolates showed hypermucoviscosity, 94.87% produced type 1 fimbriae, 92.3% produced type 3 fimbriae, and 92.3% were able to produce biofilm. In vivo persistence could be supported by serum resistance 46.15%, enterobactin (94.87%) and aerobactin (5.12%) production and invasion of the INT407 and T24 cell lines. Sequence analysis of the whole genomes of the four representative strains 11/3, 50/1, 53/2 and 53/3 has revealed high sequence homology to the reference K. pneumoniae strain HS11286. Our results represent the divergence of virulence attributes among the isolates derived from a common ancestor clone ST 15, in an evolutionary process that occurred both in the hospital and in the community.}, keywords = {Biofilm formation; klebsiella pneumoniae; clinical isolates; carbapenem-resistant; Whole-genome sequencing; virulence potential}, year = {2023}, eissn = {2079-6382} } @mastersthesis{MTMT:31808187, title = {Karbapenem rezisztens Klebsiella pneumoniae izolátumok virulencia faktorainka vizsgálata és vB_KpnS_Kp13 bakteriofág identifikálása}, url = {https://m2.mtmt.hu/api/publication/31808187}, author = {Horváth, Marianna}, unique-id = {31808187}, year = {2020} } @article{MTMT:31272367, title = {Identification of a newly isolated lytic bacteriophage against K24 capsular type, carbapenem resistant Klebsiella pneumoniae isolates}, url = {https://m2.mtmt.hu/api/publication/31272367}, author = {Horváth, Marianna and Kovács, Tamás and Koderi Valappil, Sarshad and Ábrahám, Hajnalka and Rákhely, Gábor and Schneider, György}, doi = {10.1038/s41598-020-62691-8}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {10}, unique-id = {31272367}, issn = {2045-2322}, year = {2020}, eissn = {2045-2322}, orcid-numbers = {Rákhely, Gábor/0000-0003-2557-3641} } @article{MTMT:3400835, title = {Kinetics of Targeted Phage Rescue in a Mouse Model of Systemic Escherichia coli K1}, url = {https://m2.mtmt.hu/api/publication/3400835}, author = {Schneider, György and Szentes, Nikolett and Horváth, Marianna and Dorn, Ágnes and Cox, A and Nagy, G and Doffkay, Zsolt and Maróti, Gergely and Rákhely, Gábor and Kovács, Tamás}, doi = {10.1155/2018/7569645}, journal-iso = {BIOMED RES INT}, journal = {BIOMED RESEARCH INTERNATIONAL}, volume = {2018}, unique-id = {3400835}, issn = {2314-6133}, abstract = {Escherichia (E.) coli K1 strains remain common causative agents of neonatal sepsis and meningitis. We have isolated a lytic bacteriophage (Phi IK1) against E. coli strain IHE3034 and tested its specificity in vitro, as well as distribution and protective efficacy in vivo. The phage was shown to be specific to the K1 capsular polysaccharide. In the lethal murine model, a high level of protection was afforded by the phage with strict kinetics. A single dose of 1 x 10(8) phage particles administered 10 and 60 minutes following the bacterial challenge elicited 100 % and 95 % survival, respectively. No mice could be rescued if phage administration occurred 3 hours postinfection. Tissue distribution surveys in the surviving mice revealed that the spleen was the primary organ in which accumulation of active Phi IK1 phages could be detected two weeks after phage administration. These results suggest that bacteriophages have potential as therapeutic agents in the control of systemic infections.}, keywords = {MICE; SURVIVAL; INFECTION; Therapy; Sepsis; KLEBSIELLA-PNEUMONIAE; PSEUDOMONAS-AERUGINOSA; meningitis; STAPHYLOCOCCUS-AUREUS; bacteriophage}, year = {2018}, eissn = {2314-6141}, orcid-numbers = {Maróti, Gergely/0000-0002-3705-0461; Rákhely, Gábor/0000-0003-2557-3641} } @article{MTMT:3343546, title = {Hydrothermal evolution of PF-co-doped TiO2 nanoparticles and their antibacterial activity against carbapenem-resistant Klebsiella pneumoniae}, url = {https://m2.mtmt.hu/api/publication/3343546}, author = {Kőrösi, László Tamás and Bognár, Balázs and Horváth, Marianna and Schneider, György and Kovács, János and Scarpellini, Alice and Castelli, Andrea and Colombo, Massimo and Prato, Mirko}, doi = {10.1016/j.apcatb.2018.03.012}, journal-iso = {APPL CATAL B-ENVIRON}, journal = {APPLIED CATALYSIS B-ENVIRONMENTAL}, volume = {231}, unique-id = {3343546}, issn = {0926-3373}, keywords = {TIO2; EPR; antibacterial activity; Photocatalysis; nonmetal co-doping}, year = {2018}, eissn = {1873-3883}, pages = {115-122}, orcid-numbers = {Kovács, János/0000-0001-7742-5515} } @article{MTMT:3139666, title = {Potential of small-scale jar systems to extend the shelf life of raw meats, and hinder the proliferation of Campylobacter jejuni and Enterohemorrhagic Escherichia coli}, url = {https://m2.mtmt.hu/api/publication/3139666}, author = {Kovács, Tamás and Lootz, Kinga and Dorn, Ágnes and Andrieu, Josu and Horváth, Marianna and Mátyás, Adrienne and Schneider, György}, doi = {10.1016/j.lwt.2016.10.058}, journal-iso = {LWT-FOOD SCI TECHNOL}, journal = {LWT-FOOD SCIENCE AND TECHNOLOGY}, volume = {79}, unique-id = {3139666}, issn = {0023-6438}, year = {2017}, eissn = {1096-1127}, pages = {525-533} } @article{MTMT:2916733, title = {Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital}, url = {https://m2.mtmt.hu/api/publication/2916733}, author = {Melegh, Szilvia Zsóka and Schneider, György and Horváth, Marianna and Jakab, Ferenc and Emődy, Levente and Tigyi, Zoltán}, doi = {10.1556/030.62.2015.3.2}, journal-iso = {ACTA MICROBIOL IMMUNOL HUNG}, journal = {ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA}, volume = {62}, unique-id = {2916733}, issn = {1217-8950}, year = {2015}, eissn = {1588-2640}, pages = {233-245} }