TY - JOUR AU - Szegedi, Viktor AU - Bakos , Emőke AU - Furdan, Szabina AU - H. Kovács, Bálint Barna AU - Varga, Dániel AU - Erdélyi, Miklós AU - Barzó, Pál AU - Szűcs, Attila AU - Tamás, Gábor AU - Lamsa, Karri TI - HCN channels at the cell soma ensure the rapid electrical reactivity of fast-spiking interneurons in human neocortex JF - PLOS BIOLOGY J2 - PLOS BIOL VL - 21 PY - 2023 IS - 2 PG - 33 SN - 1544-9173 DO - 10.1371/journal.pbio.3002001 UR - https://m2.mtmt.hu/api/publication/33644822 ID - 33644822 N1 - Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary Hungarian Centre of Excellence for Molecular Medicine Research Group for Human neuron physiology and therapy, Szeged, Hungary Department of Optics and Quantum Electronics, University of Szeged, Szeged, Hungary Department of Neurosurgery, University of Szeged, Szeged, Hungary Neuronal Cell Biology Research Group, Eötvös Loránd University, Budapest, Budapest, Hungary MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary Export Date: 26 May 2023 CODEN: PBLIB Correspondence Address: Lamsa, K.; Department of Physiology, Hungary; email: klamsa@bio.u-szeged.hu AB - Accumulating evidence indicates that there are substantial species differences in the properties of mammalian neurons, yet theories on circuit activity and information processing in the human brain are based heavily on results obtained from rodents and other experimental animals. This knowledge gap may be particularly important for understanding the neocortex, the brain area responsible for the most complex neuronal operations and showing the greatest evolutionary divergence. Here, we examined differences in the electrophysiological properties of human and mouse fast-spiking GABAergic basket cells, among the most abundant inhibitory interneurons in cortex. Analyses of membrane potential responses to current input, pharmacologically isolated somatic leak currents, isolated soma outside-out patch recordings, and immunohistochemical staining revealed that human neocortical basket cells abundantly express hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel isoforms HCN1 and HCN2 at the cell soma membrane, whereas these channels are sparse at the rodent basket cell soma membrane. Antagonist experiments showed that HCN channels in human neurons contribute to the resting membrane potential and cell excitability at the cell soma, accelerate somatic membrane potential kinetics, and shorten the lag between excitatory postsynaptic potentials and action potential generation. These effects are important because the soma of human fast-spiking neurons without HCN channels exhibit low persistent ion leak and slow membrane potential kinetics, compared with mouse fast-spiking neurons. HCN channels speed up human cell membrane potential kinetics and help attain an input–output rate close to that of rodent cells. Computational modeling demonstrated that HCN channel activity at the human fast-spiking cell soma membrane is sufficient to accelerate the input–output function as observed in cell recordings. Thus, human and mouse fast-spiking neurons exhibit functionally significant differences in ion channel composition at the cell soma membrane to set the speed and fidelity of their input–output function. These HCN channels ensure fast electrical reactivity of fast-spiking cells in human neocortex. LA - English DB - MTMT ER - TY - THES AU - Furdan, Szabina TI - Fiziológiás és patológiás agyi állapotok celluláris és hálózati mechanizmusai [Cellular and network mechanisms of physiological and pathological brain states] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 71 DO - 10.14232/phd.10910 UR - https://m2.mtmt.hu/api/publication/32846921 ID - 32846921 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Berg, J. AU - Sorensen, S.A. AU - Ting, J.T. AU - Miller, J.A. AU - Chartrand, T. AU - Buchin, A. AU - Bakken, T.E. AU - Budzillo, A. AU - Dee, N. AU - Ding, S.-L. AU - Gouwens, N.W. AU - Hodge, R.D. AU - Kalmbach, B. AU - Lee, C. AU - Lee, B.R. AU - Alfiler, L. AU - Baker, K. AU - Barkan, E. AU - Beller, A. AU - Berry, K. AU - Bertagnolli, D. AU - Bickley, K. AU - Bomben, J. AU - Braun, T. AU - Brouner, K. AU - Casper, T. AU - Chong, P. AU - Crichton, K. AU - Dalley, R. AU - de, Frates R. AU - Desta, T. AU - Lee, S.D. AU - D’Orazi, F. AU - Dotson, N. AU - Egdorf, T. AU - Enstrom, R. AU - Farrell, C. AU - Feng, D. AU - Fong, O. AU - Furdan, Szabina AU - Galakhova, A.A. AU - Gamlin, C. AU - Gary, A. AU - Glandon, A. AU - Goldy, J. AU - Gorham, M. AU - Goriounova, N.A. AU - Gratiy, S. AU - Graybuck, L. AU - Gu, H. AU - Hadley, K. AU - Hansen, N. AU - Heistek, T.S. AU - Henry, A.M. AU - Heyer, D.B. AU - Hill, D.J. AU - Hill, C. AU - Hupp, M. AU - Jarsky, T. AU - Kebede, S. AU - Keene, L. AU - Kim, L. AU - Kim, M.-H. AU - Kroll, M. AU - Latimer, C. AU - Levi, B.P. AU - Link, K.E. AU - Mallory, M. AU - Mann, R. AU - Marshall, D. AU - Maxwell, M. AU - McGraw, M. AU - McMillen, D. AU - Melief, E. AU - Mertens, E.J. AU - Mezei, L. AU - Mihut, Norbert AU - Mok, S. AU - Molnár, Gábor AU - Mukora, A. AU - Ng, L. AU - Ngo, K. AU - Nicovich, P.R. AU - Nyhus, J. AU - Oláh, Gáspár AU - Oldre, A. AU - Omstead, V. AU - Ozsvár, Attila AU - Park, D. AU - Peng, H. AU - Pham, T. AU - Pom, C.A. AU - Potekhina, L. AU - Rajanbabu, R. AU - Ransford, S. AU - Reid, D. AU - Rimorin, C. AU - Ruiz, A. AU - Sandman, D. AU - Sulc, J. AU - Sunkin, S.M. AU - Szafer, A. AU - Szemenyei, Viktor AU - Thomsen, E.R. AU - Tieu, M. AU - Torkelson, A. AU - Trinh, J. AU - Tung, H. AU - Wakeman, W. AU - Waleboer, F. AU - Ward, K. AU - Wilbers, R. AU - Williams, G. AU - Yao, Z. AU - Yoon, J.-G. AU - Anastassiou, C. AU - Arkhipov, A. AU - Barzó, Pál AU - Bernard, A. AU - Cobbs, C. AU - de, Witt Hamer P.C. AU - Ellenbogen, R.G. AU - Esposito, L. AU - Ferreira, M. AU - Gwinn, R.P. AU - Hawrylycz, M.J. AU - Hof, P.R. AU - Idema, S. AU - Jones, A.R. AU - Keene, C.D. AU - Ko, A.L. AU - Murphy, G.J. AU - Ng, L. AU - Ojemann, J.G. AU - Patel, A.P. AU - Phillips, J.W. AU - Silbergeld, D.L. AU - Smith, K. AU - Tasic, B. AU - Yuste, R. AU - Segev, I. AU - de, Kock C.P.J. AU - Mansvelder, H.D. AU - Tamás, Gábor AU - Zeng, H. AU - Koch, C. AU - Lein, E.S. TI - Human neocortical expansion involves glutamatergic neuron diversification JF - NATURE J2 - NATURE VL - 598 PY - 2021 IS - 7879 SP - 151 EP - 158 PG - 8 SN - 0028-0836 DO - 10.1038/s41586-021-03813-8 UR - https://m2.mtmt.hu/api/publication/32467760 ID - 32467760 N1 - Allen Institute for Brain Science, Seattle, WA, United States Department of Physiology and Biophysics, University of Washington, Seattle, WA, United States Department of Pathology, University of Washington, Seattle, WA, United States byte physics, Berlin, Germany MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy, and Neuroscience, University of Szeged, Szeged, Hungary Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit, Amsterdam, Netherlands Swedish Neuroscience Institute, Seattle, WA, United States Department of Neurosurgery, University of Szeged, Szeged, Hungary Cancer Center Amsterdam, Brain Tumor Center, Department of Neurosurgery, Amsterdam UMC, Vrije Universiteit, Amsterdam, Netherlands Department of Neurological Surgery, University of Washington, Seattle, WA, United States Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States NeuroTechnology Center, Columbia University, New York, NY, United States Edmond and Lily Safra Center for Brain Sciences and Department of Neurobiology, The Hebrew University Jerusalem, Jerusalem, Israel Cited By :1 Export Date: 26 October 2021 CODEN: NATUA Correspondence Address: Lein, E.S.; Allen Institute for Brain ScienceUnited States; email: Edl@alleninstitute.org LA - English DB - MTMT ER - TY - JOUR AU - Sere, Péter AU - Nikolett, Zsigri AU - Raffai, Tímea AU - Furdan, Szabina AU - Győri, Fanni AU - Vincenzo, Crunelli AU - Lőrincz, László Magor TI - Activity of the Lateral Hypothalamus during Genetically Determined Absence Seizures JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 17 PG - 8 SN - 1661-6596 DO - 10.3390/ijms22179466 UR - https://m2.mtmt.hu/api/publication/32167983 ID - 32167983 LA - English DB - MTMT ER - TY - JOUR AU - Gazea, Mary AU - Furdan, Szabina AU - Sere, Péter AU - Oesch, Lukas AU - Molnár, Benedek AU - Giovanni, Giuseppe Di AU - Fenno, Lief E. AU - Ramakrishnan, Charu AU - Mattis, Joanna AU - Deisseroth, Karl AU - Dymecki, Susan M. AU - Adamantidis, Antoine R. AU - Lőrincz, László Magor TI - Reciprocal lateral hypothalamic and raphé GABAergic projections promote wakefulness JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 41 PY - 2021 IS - 22 SP - 4840 EP - 4849 PG - 10 SN - 0270-6474 DO - 10.1523/JNEUROSCI.2850-20.2021 UR - https://m2.mtmt.hu/api/publication/31987689 ID - 31987689 N1 - Centre for Experimental Neurology, Department of Neurology, Inselspital, University Hospital Bern, University of Bern, Bern, 3010, Switzerland Department of Biomedical Research, Inselspital University Hospital Bern, University of Bern, Bern, 3010, Switzerland Department of Physiology, Anatomy, and Neuroscience, University of Szeged, Szeged, 6726, Hungary Department of Physiology, University of Szeged, Szeged, 6720, Hungary Neurosci ence Division, School of Bioscience, Cardiff University, Cardiff, CF10 3AX, United Kingdom Department of Physiology and Biochemistry, University of Malta, MSD 2080, Malta Departments of Psychiatry and Behavioral Sciences and Bioengineering, Stanford University, Stanford, CA 94305, United States CNC Program, Stanford University, Stanford, CA 94305, United States Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, United States Department of Genetics, Harvard Medical School, Boston, MA 02115, United States Concentris research management GmbH, Fürstenfeldbruck, D-82256, Germany Cited By :3 Export Date: 13 May 2022 CODEN: JNRSD Correspondence Address: Adamantidis, A.R.; Centre for Experimental Neurology, Switzerland; email: antoine.adamantidis@dbmr.unibe.ch. Correspondence Address: Lőrincz, M.L.; Department of Physiology, Hungary; email: mlorincz@gmail.com Funding details: National Institutes of Health, NIH, R01 DA034022 Funding details: European Research Council, ERC, 725850 Funding details: Deutsche Forschungsgemeinschaft, DFG, GA 2410/1-1, RGY0076/2012 Funding details: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNF, 31003A_156156 Funding details: Hungarian Scientific Research Fund, OTKA, FK123831, KTIA_NAP_13-2-2014-0014, NN125601 Funding details: Ministry of Culture, Sports and Tourism, MCST, N. R&I 2013-01 EPILEFREE Funding text 1: This work was supported by Hungarian Scientific Research Fund Grants NN125601 and FK123831 to M.L.L., Hungarian Brain Research Program Grant KTIA_NAP_13-2-2014-0014 to M.L.L., National Institutes of Health R01 DA034022 to S.M. D., German Research Foundation GA 2410/1-1 to M.G., Human Frontier Science Program RGY0076/2012 to A.R.A., Swiss National Science Foundation Grant 31003A_156156 to A.R.A., and European Research Council Grant 725850 to A.R.A., MCST grant N. R&I 2013-01 EPILEFREE to G.D.G and Sz.F. M.L.L. is a grantee of the János Bolyai Fellowship. This work is dedicated to the loving memory of our friend and colleague István Ábrahám. pM.G., S.F., A.R.A., and M.L.L. contributed equally to this work. LA - English DB - MTMT ER - TY - JOUR AU - Csajbók, Éva AU - Kocsis, Ágnes Katalin AU - Faragó, Nóra AU - Furdan, Szabina AU - Kovács, Balázs AU - Lovas, Sándor AU - Molnár, Gábor AU - Likó, István AU - Zvara, Ágnes AU - Puskás, László AU - Patócs, Attila Balázs AU - Tamás, Gábor TI - Expression of GLP-1 receptors in insulin-containing interneurons of rat cerebral cortex JF - DIABETOLOGIA J2 - DIABETOLOGIA VL - 62 PY - 2019 IS - 4 SP - 717 EP - 725 PG - 9 SN - 0012-186X DO - 10.1007/s00125-018-4803-z UR - https://m2.mtmt.hu/api/publication/30399139 ID - 30399139 N1 - MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :1 Export Date: 28 January 2020 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8 Funding details: GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council Funding details: 20391-3/2018/FEKUSTRAT Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :1 Export Date: 15 February 2020 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8 Funding details: GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council Funding details: 20391-3/2018/FEKUSTRAT Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :2 Export Date: 9 April 2020 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council, ERC Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :2 Export Date: 15 April 2020 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council, ERC Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 28 January 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council, ERC Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 1 February 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding details: European Research Council, ERC Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 23 February 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 18 March 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 24 March 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 1 April 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 6 May 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 17 May 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :4 Export Date: 10 August 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary 1st Department of Internal Medicine, University of Szeged, Szeged, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Avidin Ltd, Szeged, Hungary MTA Lendület Hereditary Endocrine Tumors Research Group, Semmelweis University, Budapest, Hungary Cited By :4 Export Date: 8 September 2021 CODEN: DBTGA Correspondence Address: Tamás, G.; MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Közép fasor 52, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: insulin, 9004-10-8; glucagon like peptide 1, 89750-14-1; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Insulin Funding details: European Research Council, ERC Funding details: Magyar Tudományos Akadémia, MTA Funding details: Emberi Eroforrások Minisztériuma, EMMI, 20391-3/2018/FEKUSTRAT Funding details: Aeronautical Development Agency, ADA Funding details: National Research, Development and Innovation Office, GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155 Funding text 1: Funding This work was supported by the European Research Council INTERIMPACT project (GT), the Hungarian Academy of Sciences (GT), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00018, VKSZ-14-1-2015-0155), the Ministry of Human Capacities, Hungary (grant 20391-3/2018/FEKUSTRAT) and by the National Brain Research Program, Hungary (GT). Funding text 2: The authors thank ?. T?th, M. V. V?rady and N. T?th for technical assistance (MTA-SZTE Research Group for Cortical Microcircuits, University of Szeged, Szeged, Hungary). Some of these data were presented as an abstract at the ADA 77th Scientific Sessions in 2017. AB - Aims/hypothesisGlucagon-like peptide 1 (GLP-1) receptors are expressed by pancreatic beta cells and GLP-1 receptor signalling promotes insulin secretion. GLP-1 receptor agonists have neural effects and are therapeutically promising for mild cognitive impairment and Alzheimer's disease. Our previous results showed that insulin is released by neurogliaform neurons in the cerebral cortex, but the expression of GLP-1 receptors on insulin-producing neocortical neurons has not been tested. In this study, we aimed to determine whether GLP-1 receptors are present in insulin-containing neurons.MethodsWe harvested the cytoplasm of electrophysiologically and anatomically identified neurogliaform interneurons during patch-clamp recordings performed in slices of rat neocortex. Using single-cell digital PCR, we determined copy numbers of Glp1r mRNA and other key genes in neurogliaform cells harvested in conditions corresponding to hypoglycaemia (0.5mmol/l glucose) and hyperglycaemia (10mmol/l glucose). In addition, we performed whole-cell patch-clamp recordings on neurogliaform cells to test the effects of GLP-1 receptor agonists for functional validation of single-cell digital PCR results.ResultsSingle-cell digital PCR revealed GLP-1 receptor expression in neurogliaform cells and showed that copy numbers of mRNA of the Glp1r gene in hyperglycaemia exceeded those in hypoglycaemia by 9.6 times (p<0.008). Moreover, single-cell digital PCR confirmed co-expression of Glp1r and Ins2 mRNA in neurogliaform cells. Functional expression of GLP-1 receptors was confirmed with whole-cell patch-clamp electrophysiology, showing a reversible effect of GLP-1 on neurogliaform cells. This effect was prevented by pre-treatment with the GLP-1 receptor-specific antagonist exendin-3(9-39) and was absent in hypoglycaemia. In addition, single-cell digital PCR of neurogliaform cells revealed that the expression of transcription factors (Pdx1, Isl1, Mafb) are important in beta cell development.Conclusions/interpretationOur results provide evidence for the functional expression of GLP-1 receptors in neurons known to release insulin in the cerebral cortex. Hyperglycaemia increases the expression of GLP-1 receptors in neurogliaform cells, suggesting that endogenous incretins and therapeutic GLP-1 receptor agonists might have effects on these neurons, similar to those in pancreatic beta cells. LA - English DB - MTMT ER - TY - JOUR AU - David, Francois AU - Carcak, Nihan AU - Furdan, Szabina AU - Onat, Filiz AU - Gould, Timothy AU - Mészáros, Ádám AU - Di Giovanni, Giuseppe AU - Hernandez, Vivian M AU - Chan, C Savio AU - Lőrincz, László Magor AU - Crunelli, Vincenzo TI - Suppression of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Function in Thalamocortical Neurons Prevents Genetically Determined and Pharmacologically Induced Absence Seizures JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 38 PY - 2018 IS - 30 SP - 6615 EP - 6627 PG - 13 SN - 0270-6474 DO - 10.1523/JNEUROSCI.0896-17.2018 UR - https://m2.mtmt.hu/api/publication/27581009 ID - 27581009 N1 - Neuroscience Division, School of Biosciences, Cardiff University, Cardiff, CF10 3AX, United Kingdom Lyon Neuroscience Research Center, CNRS, UMR 5292-INSERM U1028, Université Claude Bernard, Lyon, 69008, France Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey Department of Physiology, Anatomy, and Neuroscience, University of Szeged, Szeged, 6726, Hungary Department of Pharmacology and Clinical 34452 Pharmacology, Marmara University School of Medicine, Istanbul, 81326, Turkey Department of Physiology and Biochemistry, University of Malta, Msida, MSD 2080, Malta Department of Physiology, Feinberg School of Medicine, Northwestern University, Robert H Lurie Medical Research Center, Chicago, IL 60611, United States Cited By :1 Export Date: 8 January 2019 CODEN: JNRSD Correspondence Address: David, F.; Lyon Neuroscience Research Center, 8, Avenue Rockefeller, France; email: francois.david@inserm.fr LA - English DB - MTMT ER - TY - CONF AU - Furdan, Szabina AU - Lőrincz, László Magor TI - Origin of olfactory inputs to brainstem dorsal raphé nucleus neurons T2 - 1st Hungarian Neuroscience Doctoral Conference C1 - Budapest PY - 2016 SP - 16 EP - 16 PG - 1 UR - https://m2.mtmt.hu/api/publication/3086329 ID - 3086329 N1 - előadás LA - English DB - MTMT ER - TY - JOUR AU - Furdan, Szabina AU - Lőrincz, László Magor TI - Routing of olfactory inputs to the brainstem raphé nucleus JF - CLINICAL NEUROPHYSIOLOGY J2 - CLIN NEUROPHYSIOL VL - 127 PY - 2016 IS - 3 PG - 1 SN - 1388-2457 DO - 10.1016/j.clinph.2015.11.417 UR - https://m2.mtmt.hu/api/publication/3085614 ID - 3085614 LA - English DB - MTMT ER - TY - CONF AU - Furdan, Szabina AU - Lőrincz, László Magor TI - Origin of olfactory inputs to brainstem dorsal raphé nucleus neurons T2 - IBRO Workshop 2016 PY - 2016 SP - 43 EP - 44 PG - 2 UR - https://m2.mtmt.hu/api/publication/3085606 ID - 3085606 LA - English DB - MTMT ER -