TY - JOUR AU - Farkas, Dávid AU - Szikora, Szilárd AU - Jijumon, A S AU - Polgár, Tamás Ferenc AU - Patai, Roland AU - Szütsné Tóth, Mónika Ágnes AU - Bugyi, Beáta AU - Gajdos, Tamás AU - Bíró, Péter AU - Novák, Tibor AU - Erdélyi, Miklós AU - Mihály, József TI - Peripheral thickening of the sarcomeres and pointed end elongation of the thin filaments are both promoted by SALS and its formin interaction partners JF - PLOS GENETICS J2 - PLOS GENET VL - 20 PY - 2024 IS - 1 PG - 31 SN - 1553-7390 DO - 10.1371/journal.pgen.1011117 UR - https://m2.mtmt.hu/api/publication/34506131 ID - 34506131 AB - During striated muscle development the first periodically repeated units appear in the premyofibrils, consisting of immature sarcomeres that must undergo a substantial growth both in length and width, to reach their final size. Here we report that, beyond its well established role in sarcomere elongation, the Sarcomere length short (SALS) protein is involved in Z-disc formation and peripheral growth of the sarcomeres. Our protein localization data and loss-of-function studies in the Drosophila indirect flight muscle strongly suggest that radial growth of the sarcomeres is initiated at the Z-disc. As to thin filament elongation, we used a powerful nanoscopy approach to reveal that SALS is subject to a major conformational change during sarcomere development, which might be critical to stop pointed end elongation in the adult muscles. In addition, we demonstrate that the roles of SALS in sarcomere elongation and radial growth are both dependent on formin type of actin assembly factors. Unexpectedly, when SALS is present in excess amounts, it promotes the formation of actin aggregates highly resembling the ones described in nemaline myopathy patients. Collectively, these findings helped to shed light on the complex mechanisms of SALS during the coordinated elongation and thickening of the sarcomeres, and resulted in the discovery of a potential nemaline myopathy model, suitable for the identification of genetic and small molecule inhibitors. LA - English DB - MTMT ER - TY - THES AU - Gajdos, Tamás TI - Nagyfeloldású lokalizációs mikroszkópia több modalitásban [Superresolution localization microscopy using multiple modalities] PB - Szegedi Tudományegyetem (SZTE) PY - 2020 SP - 105 DO - 10.14232/phd.10417 UR - https://m2.mtmt.hu/api/publication/31957903 ID - 31957903 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szikora, Szilárd AU - Novák, Tibor AU - Gajdos, Tamás AU - Erdélyi, Miklós AU - Mihály, József TI - Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres JF - BIO-PROTOCOL J2 - BIO-PROTOCOL VL - 10 PY - 2020 IS - 12 PG - 16 SN - 2331-8325 DO - 10.21769/BioProtoc.3654 UR - https://m2.mtmt.hu/api/publication/31661623 ID - 31661623 AB - Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial arrangement of many of the H-zone and Z-disk proteins remained unknown. Recently, we developed a powerful nanoscopic approach to localize the sarcomeric protein components with a resolution well below the diffraction limit. The ease of sample preparation and the near crystalline structure of the Drosophila flight muscle sarcomeres make them ideally suitable for single molecule localization microscopy and structure averaging. Our approach allowed us to determine the position of dozens of H-zone and Z-disk proteins with a quasi-molecular, similar to 5-10 nm localization precision. The protocol described below provides an easy and reproducible method to prepare individual myofibrils for dSTORM imaging. In addition, it includes an in-depth description of a custom made and freely available software toolbox to process and quantitatively analyze the raw localization data. LA - English DB - MTMT ER - TY - CHAP AU - Köhler, Zoltán Márton AU - Juhász, László AU - Trencsényi, György AU - Mária, Péter AU - Gajdos, Tamás AU - Erdélyi, Miklós AU - László, Vígh AU - Gábor, Balogh AU - Dux, László AU - Keller-Pintér, Anikó ED - Rakonczay, Zoltán ED - Kiss, Lóránd TI - New mechanism of glucose uptake in skeletal muscle T2 - Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project PB - University of Szeged CY - Szeged SN - 9789633067642 PY - 2020 SP - 94 UR - https://m2.mtmt.hu/api/publication/31636102 ID - 31636102 LA - English DB - MTMT ER - TY - JOUR AU - Becsky, Dániel AU - Szabó, Kitti AU - Fazekas, Szuzina AU - Gajdos, Tamás AU - Bartos, Zsuzsa AU - Bálind, Árpád AU - Dux, László AU - Horváth, Péter AU - Erdélyi, Miklós AU - Homolya, László AU - Keller-Pintér, Anikó TI - Syndecan-4 Modulates Cell Polarity and Migration by Influencing Centrosome Positioning and Intracellular Calcium Distribution JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 8 PY - 2020 PG - 17 SN - 2296-634X DO - 10.3389/fcell.2020.575227 UR - https://m2.mtmt.hu/api/publication/31634527 ID - 31634527 LA - English DB - MTMT ER - TY - JOUR AU - Gajdos, Tamás AU - Hopp, Béla AU - Erdélyi, Miklós TI - Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568 JF - JOURNAL OF FLUORESCENCE J2 - J FLUORESC VL - 30 PY - 2020 IS - 3 SP - 437 EP - 443 PG - 7 SN - 1053-0509 DO - 10.1007/s10895-020-02496-0 UR - https://m2.mtmt.hu/api/publication/31202130 ID - 31202130 N1 - Export Date: 4 May 2020 CODEN: JOFLE Correspondence Address: Erdélyi, M.; Department of Optics and Quantum Electronics, University of Szeged, Dóm tér 9., Hungary; email: erdelyi@titan.physx.u-szeged.hu Funding details: EFOP-3.6.1-16-2016-00008 Funding text 1: This work was supported by the Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002); the GINOP-2.3.2-15-2016-00036; the EU-funded Hungarian Grant EFOP-3.6.1-16-2016-00008. We thank Gábor Laczkó for a fruitful discussion. We would like to acknowledge József Mihály and Szilárd Szikora for providing the drosophila myofibril sample. Open access funding provided by University of Szeged (SZTE). LA - English DB - MTMT ER - TY - JOUR AU - Szikora, Szilárd AU - Gajdos, Tamás AU - Novák, Tibor AU - Farkas, Dávid AU - Földi, István AU - Lenart, Peter AU - Erdélyi, Miklós AU - Mihály, József TI - Nanoscopy reveals the layered organization of the sarcomeric H-zone and I-band complexes JF - JOURNAL OF CELL BIOLOGY J2 - J CELL BIOL VL - 219 PY - 2020 IS - 1 PG - 21 SN - 0021-9525 DO - 10.1083/jcb.201907026 UR - https://m2.mtmt.hu/api/publication/30993210 ID - 30993210 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Optics and Quantum Electronics, University of Szeged, Szeged, Hungary Max Planck Institute for Biophysical Chemistry, Göttingen, Germany Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Cited By :5 Export Date: 21 January 2021 CODEN: JCLBA Correspondence Address: Mihály, J.; Institute of Genetics, Hungary; email: mihaly.jozsef@brc.hu LA - English DB - MTMT ER - TY - CONF AU - Becsky, Dániel AU - Szabó, Kitti AU - Gajdos, Tamás AU - Bálind, Árpád AU - Horváth, Péter AU - Erdélyi, Miklós AU - Dux, László AU - Keller-Pintér, Anikó AU - Fazekas, Szuzina TI - A Szindekán-4 szerepe a mioblasztok migrációjában T2 - 49. Membrán-transzport Konferencia, Sümeg, 2019.05.14-17 PB - Remedicon PY - 2019 EP - 4 PG - 1 UR - https://m2.mtmt.hu/api/publication/31289837 ID - 31289837 LA - Hungarian DB - MTMT ER - TY - CONF AU - Köhler, Zoltán Márton AU - Juhász, László AU - Gajdos, Tamás AU - Erdélyi, Miklós AU - Trencsényi, György AU - Dux, László AU - Keller-Pintér, Anikó ED - Magyar, Genetikusok Egyesülete TI - Metabolikus és mitokondriális változások syndecan-4 csendesített vázizomsejtekben T2 - III. Sejt-, Fejlődés- és Őssejtbiológusok Éves Találkozójának programja és absztraktjai PY - 2019 SP - 38 UR - https://m2.mtmt.hu/api/publication/31164187 ID - 31164187 LA - Hungarian DB - MTMT ER - TY - CONF AU - Köhler, Zoltán Márton AU - Juhász, László AU - Gajdos, Tamás AU - Erdélyi, Miklós AU - Trencsényi, György AU - Dux, László AU - Keller-Pintér, Anikó TI - A szindekán-4 proteoglikán hatása a vázizomsejtek glükózfelvételére és mitokondriális légzésére T2 - 49. Membrán-transzport Konferencia, Sümeg, 2019.05.14-17 PB - Remedicon PY - 2019 PG - 1 UR - https://m2.mtmt.hu/api/publication/31164146 ID - 31164146 LA - Hungarian DB - MTMT ER -