@article{MTMT:34506131, title = {Peripheral thickening of the sarcomeres and pointed end elongation of the thin filaments are both promoted by SALS and its formin interaction partners}, url = {https://m2.mtmt.hu/api/publication/34506131}, author = {Farkas, Dávid and Szikora, Szilárd and Jijumon, A S and Polgár, Tamás Ferenc and Patai, Roland and Szütsné Tóth, Mónika Ágnes and Bugyi, Beáta and Gajdos, Tamás and Bíró, Péter and Novák, Tibor and Erdélyi, Miklós and Mihály, József}, doi = {10.1371/journal.pgen.1011117}, journal-iso = {PLOS GENET}, journal = {PLOS GENETICS}, volume = {20}, unique-id = {34506131}, issn = {1553-7390}, abstract = {During striated muscle development the first periodically repeated units appear in the premyofibrils, consisting of immature sarcomeres that must undergo a substantial growth both in length and width, to reach their final size. Here we report that, beyond its well established role in sarcomere elongation, the Sarcomere length short (SALS) protein is involved in Z-disc formation and peripheral growth of the sarcomeres. Our protein localization data and loss-of-function studies in the Drosophila indirect flight muscle strongly suggest that radial growth of the sarcomeres is initiated at the Z-disc. As to thin filament elongation, we used a powerful nanoscopy approach to reveal that SALS is subject to a major conformational change during sarcomere development, which might be critical to stop pointed end elongation in the adult muscles. In addition, we demonstrate that the roles of SALS in sarcomere elongation and radial growth are both dependent on formin type of actin assembly factors. Unexpectedly, when SALS is present in excess amounts, it promotes the formation of actin aggregates highly resembling the ones described in nemaline myopathy patients. Collectively, these findings helped to shed light on the complex mechanisms of SALS during the coordinated elongation and thickening of the sarcomeres, and resulted in the discovery of a potential nemaline myopathy model, suitable for the identification of genetic and small molecule inhibitors.}, year = {2024}, eissn = {1553-7404}, orcid-numbers = {Gajdos, Tamás/0000-0002-5288-4659; Novák, Tibor/0000-0003-0756-6851; Erdélyi, Miklós/0000-0002-9501-5752} } @mastersthesis{MTMT:31957903, title = {Nagyfeloldású lokalizációs mikroszkópia több modalitásban [Superresolution localization microscopy using multiple modalities]}, url = {https://m2.mtmt.hu/api/publication/31957903}, author = {Gajdos, Tamás}, doi = {10.14232/phd.10417}, publisher = {SZTE}, unique-id = {31957903}, year = {2020}, orcid-numbers = {Gajdos, Tamás/0000-0002-5288-4659} } @article{MTMT:31661623, title = {Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres}, url = {https://m2.mtmt.hu/api/publication/31661623}, author = {Szikora, Szilárd and Novák, Tibor and Gajdos, Tamás and Erdélyi, Miklós and Mihály, József}, doi = {10.21769/BioProtoc.3654}, journal-iso = {BIO-PROTOCOL}, journal = {BIO-PROTOCOL}, volume = {10}, unique-id = {31661623}, issn = {2331-8325}, abstract = {Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial arrangement of many of the H-zone and Z-disk proteins remained unknown. Recently, we developed a powerful nanoscopic approach to localize the sarcomeric protein components with a resolution well below the diffraction limit. The ease of sample preparation and the near crystalline structure of the Drosophila flight muscle sarcomeres make them ideally suitable for single molecule localization microscopy and structure averaging. Our approach allowed us to determine the position of dozens of H-zone and Z-disk proteins with a quasi-molecular, similar to 5-10 nm localization precision. The protocol described below provides an easy and reproducible method to prepare individual myofibrils for dSTORM imaging. In addition, it includes an in-depth description of a custom made and freely available software toolbox to process and quantitatively analyze the raw localization data.}, year = {2020}, orcid-numbers = {Novák, Tibor/0000-0003-0756-6851; Gajdos, Tamás/0000-0002-5288-4659; Erdélyi, Miklós/0000-0002-9501-5752} } @{MTMT:31636102, title = {New mechanism of glucose uptake in skeletal muscle}, url = {https://m2.mtmt.hu/api/publication/31636102}, author = {Köhler, Zoltán Márton and Juhász, László and Trencsényi, György and Mária, Péter and Gajdos, Tamás and Erdélyi, Miklós and László, Vígh and Gábor, Balogh and Dux, László and Keller-Pintér, Anikó}, booktitle = {Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project}, unique-id = {31636102}, year = {2020}, pages = {94}, orcid-numbers = {Köhler, Zoltán Márton/0000-0002-8299-105X; Juhász, László/0000-0001-5477-9572; Trencsényi, György/0000-0001-6456-6212; Gajdos, Tamás/0000-0002-5288-4659; Erdélyi, Miklós/0000-0002-9501-5752; Dux, László/0000-0002-1270-1678; Keller-Pintér, Anikó/0000-0002-4105-8458} } @article{MTMT:31634527, title = {Syndecan-4 Modulates Cell Polarity and Migration by Influencing Centrosome Positioning and Intracellular Calcium Distribution}, url = {https://m2.mtmt.hu/api/publication/31634527}, author = {Becsky, Dániel and Szabó, Kitti and Fazekas, Szuzina and Gajdos, Tamás and Bartos, Zsuzsa and Bálind, Árpád and Dux, László and Horváth, Péter and Erdélyi, Miklós and Homolya, László and Keller-Pintér, Anikó}, doi = {10.3389/fcell.2020.575227}, journal-iso = {FRONT CELL DEV BIOL}, journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY}, volume = {8}, unique-id = {31634527}, issn = {2296-634X}, year = {2020}, eissn = {2296-634X}, orcid-numbers = {Becsky, Dániel/0000-0002-7970-6000; Szabó, Kitti/0000-0002-1177-2036; Gajdos, Tamás/0000-0002-5288-4659; Bartos, Zsuzsa/0000-0001-9695-1422; Dux, László/0000-0002-1270-1678; Erdélyi, Miklós/0000-0002-9501-5752; Homolya, László/0000-0003-1639-8140; Keller-Pintér, Anikó/0000-0002-4105-8458} } @article{MTMT:31202130, title = {Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568}, url = {https://m2.mtmt.hu/api/publication/31202130}, author = {Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós}, doi = {10.1007/s10895-020-02496-0}, journal-iso = {J FLUORESC}, journal = {JOURNAL OF FLUORESCENCE}, volume = {30}, unique-id = {31202130}, issn = {1053-0509}, year = {2020}, eissn = {1573-4994}, pages = {437-443}, orcid-numbers = {Gajdos, Tamás/0000-0002-5288-4659; Hopp, Béla/0000-0002-4272-7721; Erdélyi, Miklós/0000-0002-9501-5752} } @article{MTMT:30993210, title = {Nanoscopy reveals the layered organization of the sarcomeric H-zone and I-band complexes}, url = {https://m2.mtmt.hu/api/publication/30993210}, author = {Szikora, Szilárd and Gajdos, Tamás and Novák, Tibor and Farkas, Dávid and Földi, István and Lenart, Peter and Erdélyi, Miklós and Mihály, József}, doi = {10.1083/jcb.201907026}, journal-iso = {J CELL BIOL}, journal = {JOURNAL OF CELL BIOLOGY}, volume = {219}, unique-id = {30993210}, issn = {0021-9525}, year = {2020}, eissn = {1540-8140}, orcid-numbers = {Gajdos, Tamás/0000-0002-5288-4659; Novák, Tibor/0000-0003-0756-6851; Erdélyi, Miklós/0000-0002-9501-5752} } @CONFERENCE{MTMT:31289837, title = {A Szindekán-4 szerepe a mioblasztok migrációjában}, url = {https://m2.mtmt.hu/api/publication/31289837}, author = {Becsky, Dániel and Szabó, Kitti and Gajdos, Tamás and Bálind, Árpád and Horváth, Péter and Erdélyi, Miklós and Dux, László and Keller-Pintér, Anikó and Fazekas, Szuzina}, booktitle = {49. Membrán-transzport Konferencia, Sümeg, 2019.05.14-17}, unique-id = {31289837}, year = {2019}, pages = {3-4}, orcid-numbers = {Becsky, Dániel/0000-0002-7970-6000; Szabó, Kitti/0000-0002-1177-2036; Gajdos, Tamás/0000-0002-5288-4659; Dux, László/0000-0002-1270-1678; Keller-Pintér, Anikó/0000-0002-4105-8458} } @CONFERENCE{MTMT:31164187, title = {Metabolikus és mitokondriális változások syndecan-4 csendesített vázizomsejtekben}, url = {https://m2.mtmt.hu/api/publication/31164187}, author = {Köhler, Zoltán Márton and Juhász, László and Gajdos, Tamás and Erdélyi, Miklós and Trencsényi, György and Dux, László and Keller-Pintér, Anikó}, booktitle = {III. Sejt-, Fejlődés- és Őssejtbiológusok Éves Találkozójának programja és absztraktjai}, unique-id = {31164187}, year = {2019}, pages = {38}, orcid-numbers = {Köhler, Zoltán Márton/0000-0002-8299-105X; Juhász, László/0000-0001-5477-9572; Gajdos, Tamás/0000-0002-5288-4659; Erdélyi, Miklós/0000-0002-9501-5752; Trencsényi, György/0000-0001-6456-6212; Dux, László/0000-0002-1270-1678; Keller-Pintér, Anikó/0000-0002-4105-8458} } @CONFERENCE{MTMT:31164146, title = {A szindekán-4 proteoglikán hatása a vázizomsejtek glükózfelvételére és mitokondriális légzésére}, url = {https://m2.mtmt.hu/api/publication/31164146}, author = {Köhler, Zoltán Márton and Juhász, László and Gajdos, Tamás and Erdélyi, Miklós and Trencsényi, György and Dux, László and Keller-Pintér, Anikó}, booktitle = {49. Membrán-transzport Konferencia, Sümeg, 2019.05.14-17}, unique-id = {31164146}, year = {2019}, orcid-numbers = {Köhler, Zoltán Márton/0000-0002-8299-105X; Juhász, László/0000-0001-5477-9572; Gajdos, Tamás/0000-0002-5288-4659; Erdélyi, Miklós/0000-0002-9501-5752; Trencsényi, György/0000-0001-6456-6212; Dux, László/0000-0002-1270-1678; Keller-Pintér, Anikó/0000-0002-4105-8458} }