TY - JOUR AU - Bruszel, Bella AU - Tóth-Molnár, Edit AU - Janáky, Tamás AU - Szabó, Zoltán TI - Sources of Variance in Human Tear Proteomic Samples: Statistical Evaluation, Quality Control, Normalization, and Biological Insight JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 PG - 19 SN - 1661-6596 DO - 10.3390/ijms25031559 UR - https://m2.mtmt.hu/api/publication/34536265 ID - 34536265 AB - Human tear fluid contains numerous compounds, which are present in highly variable amounts owing to the dynamic and multipurpose functions of tears. A better understanding of the level and sources of variance is essential for determining the functions of the different tear components and the limitations of tear samples as a potential biomarker source. In this study, a quantitative proteomic method was used to analyze variations in the tear protein profiles of healthy volunteers. High day-to-day and inter-eye personal variances were observed in the tear volumes, protein content, and composition of the tear samples. Several normalization and outlier exclusion approaches were evaluated to decrease variances. Despite the intrapersonal variances, statistically significant differences and cluster analysis revealed that proteome profile and immunoglobulin composition of tear fluid present personal characteristics. Using correlation analysis, we could identify several correlating protein clusters, mainly related to the source of the proteins. Our study is the first attempt to achieve more insight into the biochemical background of human tears by statistical evaluation of the experimentally observed dynamic behavior of the tear proteome. As a pilot study for determination of personal protein profiles of the tear fluids of individual patients, it contributes to the application of this noninvasively collectible body fluid in personal medicine. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Édua AU - Ali, Hazhmat AU - Minorics, Renáta AU - Traj, Péter AU - Resch, Vivien Erzsébet AU - Paragi, Gábor AU - Bruszel, Bella AU - Zupkó, István AU - Mernyák, Erzsébet TI - Synthesis and Antiproliferative Activity of Steroidal Diaryl Ethers JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 3 PG - 17 SN - 1420-3049 DO - 10.3390/molecules28031196 UR - https://m2.mtmt.hu/api/publication/33594496 ID - 33594496 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office-NKFIH [OTKA SNN 139323, TKP2021-EGA-17] Funding text: This work was supported by National Research, Development and Innovation Office-NKFIH through projects OTKA SNN 139323 and TKP2021-EGA-17. AB - Novel 13α-estrone derivatives have been synthesized via direct arylation of the phenolic hydroxy function. Chan–Lam couplings of arylboronic acids with 13α-estrone as a nucleophilic partner were carried out under copper catalysis. The antiproliferative activities of the newly synthesized diaryl ethers against a panel of human cancer cell lines (A2780, MCF-7, MDA-MB 231, HeLa, SiHa) were investigated by means of MTT assays. The quinoline derivative displayed substantial antiproliferative activity against MCF-7 and HeLa cell lines with low micromolar IC50 values. Disturbance of tubulin polymerization has been confirmed by microplate-based photometric assay. Computational calculations reveal significant interactions of the quinoline derivative with the taxoid binding site of tubulin. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Márton Richárd AU - Pipicz, Márton AU - Sárközy, Márta AU - Bruszel, Bella AU - Szabó, Zoltán AU - Csont, Tamás Bálint TI - Diet-Induced Hypercholesterolemia Leads to Cardiac Dysfunction and Alterations in the Myocardial Proteome JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 13 PG - 17 SN - 1661-6596 DO - 10.3390/ijms23137387 UR - https://m2.mtmt.hu/api/publication/32924828 ID - 32924828 N1 - Export Date: 18 October 2022 AB - Elevated blood cholesterol is a major risk factor for coronary heart disease. Moreover, direct effects on the myocardium also contribute to the adverse effects of hypercholesterolemia. Here, we investigated the effect of hypercholesterolemia on the cardiac proteome. Male Wistar rats were fed with a laboratory rodent chow supplemented with 2% cholesterol for 8 weeks to induce hypercholesterolemia. The protein expression data obtained from the proteomic characterization of left ventricular samples from normo- and hypercholesterolemic animals were subjected to gene ontology (GO) and protein interaction analyses. Elevated circulating cholesterol levels were accompanied by diastolic dysfunction in cholesterol-fed rats. The proteomic characterization of left ventricular samples revealed altered expression of 45 proteins due to hypercholesterolemia. Based on the Gene Ontology analysis, hypercholesterolemia was associated with disturbed expression of cytoskeletal and contractile proteins. Beta-actin was downregulated in the hypercholesterolemic myocardium, and established a prominent hub of the protein interaction network. Analysis of the unfiltered dataset revealed concordant downregulated expression patterns in proteins associated with the arrangement of the contractile system (e.g., cardiac-specific troponins and myosin complex), and in subunits of the mitochondrial respiratory chain. We conclude that the observed changes in the cardiac proteome may contribute to the development of diastolic dysfunction in hypercholesterolemia. LA - English DB - MTMT ER - TY - JOUR AU - Virók, Dezső AU - Tömösi, Ferenc AU - Keller-Pintér, Anikó AU - Szabó, Kitti AU - Bogdanov, Anita AU - Póliska, Szilárd AU - Rázga, Zsolt AU - Bruszel, Bella AU - Cseh, Zsuzsanna AU - Kókai, Dávid AU - Paróczai, Dóra AU - Endrész, Valéria AU - Janáky, Tamás AU - Burián, Katalin TI - Indoleamine 2,3-Dioxygenase Cannot Inhibit Chlamydia trachomatis Growth in HL-60 Human Neutrophil Granulocytes JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2021.717311 UR - https://m2.mtmt.hu/api/publication/32489937 ID - 32489937 N1 - FUNDING: DV, TJ, FT, and BB were supported by EFOP 3.6.1 Program. TJ, FT, and BB were supported by the Thematic Excellence Program 2020 (TKP2020-IKA-07). LA - English DB - MTMT ER - TY - JOUR AU - Mernyák, Erzsébet AU - Bartha, Sándor AU - Kóczán , Lili AU - Jójárt, Rebeka AU - Resch, Vivien Erzsébet AU - Paragi, Gábor AU - Vágvölgyi, Máté AU - Hunyadi, Attila AU - Bruszel, Bella AU - Zupkó, István AU - Minorics, Renáta TI - Microwave-assisted Phospha-Michael addition reactions in the 13alpha-oestrone series and in vitro antiproliferative properties JF - JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY J2 - J ENZYM INHIB MED CH VL - 36 PY - 2021 IS - 1 SP - 1931 EP - 1937 PG - 7 SN - 1475-6366 DO - 10.1080/14756366.2021.1963241 UR - https://m2.mtmt.hu/api/publication/32161034 ID - 32161034 LA - English DB - MTMT ER - TY - CHAP AU - Szűcs, Gergő AU - Apjok-Sója, Andrea AU - Péter, Mária AU - Sárközy, Márta AU - Bruszel, Bella AU - Siska, Andrea AU - Földesi, Imre AU - Szabó, Zoltán AU - Janáky, Tamás AU - László, Vígh AU - Gábor, Balogh AU - Csont, Tamás Bálint ED - Rakonczay, Zoltán ED - Kiss, Lóránd TI - Prediabetes induced by fructose-enriched diet influences cardiac lipidome and proteome and leads to deterioration of cardiac function prior to the development of excessive oxidative stress and cell damage T2 - Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project PB - University of Szeged CY - Szeged SN - 9789633067642 PY - 2020 SP - 73 EP - 73 PG - 1 UR - https://m2.mtmt.hu/api/publication/31647876 ID - 31647876 LA - English DB - MTMT ER - TY - JOUR AU - Dobra, Gabriella AU - Bukva, Mátyás AU - Szabó, Zoltán AU - Bruszel, Bella AU - Harmati, Mária AU - Gyukity-Sebestyén, Edina AU - Jenei, Adrienn AU - Szűcs, Mónika AU - Horváth, Péter AU - Bíró, Tamás AU - Klekner, Álmos AU - Buzás, Krisztina TI - Small Extracellular Vesicles Isolated from Serum May Serve as Signal-Enhancers for the Monitoring of CNS Tumors JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 15 PG - 20 SN - 1661-6596 DO - 10.3390/ijms21155359 UR - https://m2.mtmt.hu/api/publication/31390897 ID - 31390897 N1 - Funding Agency and Grant Number: National Brain Research Program NAP 2.0 [2017-1.2.1-NKP-2017-00002]; Janos Bolyai Research Scholarship of the Hungarian Academic of Sciences; [GINOP-2.3.2-15-2016-00015]; [GINOP-2.2.1-15-2017-00052]; [UNKP-19-4-SZTE-63] Funding text: This study was founded by the following research grants: GINOP-2.3.2-15-2016-00015 (K.B.); GINOP-2.2.1-15-2017-00052 (K.B.), 2017-1.2.1-NKP-2017-00002 "National Brain Research Program NAP 2.0" (A.K.), UNKP-19-4-SZTE-63 (K.B.), Janos Bolyai Research Scholarship of the Hungarian Academic of Sciences (K.B.). Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, H-6726, Hungary Department of Medical Genetics, Doctoral School of Interdisciplinary Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Neurosurgery, Clinical Centre, University of Debrecen, Debrecen, H-4032, Hungary Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Physics and Informatics, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Immunology, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Export Date: 28 August 2020 Correspondence Address: Buzas, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Department of Immunology, Faculty of Medicine, University of Szeged, Department of Immunology, Faculty of Science and Informatics, University of SzegedHungary; email: kr.buzas@gmail.com Funding text 1: Funding: This study was founded by the following research grants: GINOP-2.3.2-15-2016-00015 (K.B.); GINOP-2.2.1-15-2017-00052 (K.B.), 2017-1.2.1-NKP-2017-00002 “National Brain Research Program NAP 2.0” (A.K.), UNKP-19-4-SZTE-63 (K.B.), Janos Bolyai Research Scholarship of the Hungarian Academic of Sciences (K.B.). Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, H-6726, Hungary Department of Medical Genetics, Doctoral School of Interdisciplinary Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Neurosurgery, Clinical Centre, University of Debrecen, Debrecen, H-4032, Hungary Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Physics and Informatics, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Immunology, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Export Date: 10 January 2021 Correspondence Address: Buzas, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Department of Immunology, Faculty of Medicine, University of Szeged, Department of Immunology, Faculty of Science and Informatics, University of SzegedHungary; email: kr.buzas@gmail.com Funding text 1: Funding: This study was founded by the following research grants: GINOP-2.3.2-15-2016-00015 (K.B.); GINOP-2.2.1-15-2017-00052 (K.B.), 2017-1.2.1-NKP-2017-00002 “National Brain Research Program NAP 2.0” (A.K.), UNKP-19-4-SZTE-63 (K.B.), Janos Bolyai Research Scholarship of the Hungarian Academic of Sciences (K.B.). Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Szeged, H-6726, Hungary Department of Medical Genetics, Doctoral School of Interdisciplinary Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Neurosurgery, Clinical Centre, University of Debrecen, Debrecen, H-4032, Hungary Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Medical Physics and Informatics, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Immunology, Faculty of Medicine, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Export Date: 10 March 2021 Correspondence Address: Buzas, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Hungary; email: kr.buzas@gmail.com Correspondence Address: Buzas, K.; Department of Immunology, Hungary; email: kr.buzas@gmail.com Correspondence Address: Buzas, K.; Department of Immunology, Hungary; email: kr.buzas@gmail.com Funding text 1: Funding: This study was founded by the following research grants: GINOP-2.3.2-15-2016-00015 (K.B.); GINOP-2.2.1-15-2017-00052 (K.B.), 2017-1.2.1-NKP-2017-00002 “National Brain Research Program NAP 2.0” (A.K.), UNKP-19-4-SZTE-63 (K.B.), Janos Bolyai Research Scholarship of the Hungarian Academic of Sciences (K.B.). LA - English DB - MTMT ER - TY - JOUR AU - Bukva, Mátyás AU - Dobra, Gabriella AU - Szabó, Zoltán AU - Bruszel, Bella AU - Zsolt, Szegletes AU - András, Kincses AU - Edina, Gyukity-Sebestyén AU - Mária, Harmati AU - Álmos, Klekner AU - Buzás, Krisztina TI - Extracellular vesicles: promising source of glioblastoma biomarkers JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 11 PY - 2019 IS - 3 SP - 24 EP - 24 PG - 1 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/31522265 ID - 31522265 LA - English DB - MTMT ER - TY - JOUR AU - Szűcs, Gergő AU - Apjok-Sója, Andrea AU - Péter, Mária AU - Sárközy, Márta AU - Bruszel, Bella AU - Siska, Andrea AU - Földesi, Imre AU - Szabó, Zoltán AU - Janáky, Tamás AU - Vigh, László AU - Balogh, Gábor AU - Csont, Tamás Bálint TI - Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage JF - OXIDATIVE MEDICINE AND CELLULAR LONGEVITY J2 - OXID MED CELL LONGEV VL - 2019 PY - 2019 PG - 21 SN - 1942-0900 DO - 10.1155/2019/3218275 UR - https://m2.mtmt.hu/api/publication/31009490 ID - 31009490 N1 - Cited By :16 Export Date: 20 September 2022 LA - English DB - MTMT ER - TY - GEN AU - Bruszel, Bella AU - Zoltan, Szabo AU - Janáky, Tamás ED - Stephen, R Pennington TI - Lacrimal proteome: possible source of biomarkers? PY - 2017 SP - 428 PG - 1 UR - https://m2.mtmt.hu/api/publication/3336877 ID - 3336877 LA - English DB - MTMT ER -