TY - JOUR AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, István László AU - Kitahata, Hiroyuki AU - Suematsu, Nobuhiko J. AU - Horváth, Róbert TI - Kinetic monitoring of molecular interactions during surfactant-driven self-propelled droplet motion by high spatial resolution waveguide sensing JF - JOURNAL OF COLLOID AND INTERFACE SCIENCE J2 - J COLLOID INTERF SCI VL - 677 PY - 2025 SP - 352 EP - 364 PG - 13 SN - 0021-9797 DO - 10.1016/j.jcis.2024.07.236 UR - https://m2.mtmt.hu/api/publication/35173267 ID - 35173267 AB - Hypothesis: Self-driven actions, like motion, are fundamental characteristics of life. Today, intense research focuses on the kinetics of droplet motion. Quantifying macroscopic motion and exploring the underlying mechanisms are crucial in self-structuring and self-healing materials, advancements in soft robotics, innovations in self-cleaning environmental processes, and progress within the pharmaceutical industry. Usually, the driving forces inducing macroscopic motion act at the molecular scale, making their real-time and high-resolution investigation challenging. Label-free surface sensitive measurements with high lateral resolution could in situ measure both molecular-scale interactions and microscopic motion. Experiments: We employ surface-sensitive label-free sensors to investigate the kinetic changes in a self-assembled monolayer of the trimethyl(octadecyl)azanium chloride surfactant on a substrate surface during the self-propelled motion of nitrobenzene droplets. The adsorption–desorption of the surfactant at various concentrations, its removal due to the moving organic droplet, and rebuilding mechanisms at droplet-visited areas are all investigated with excellent time, spatial, and surface mass density resolution. Findings: We discovered concentration dependent velocity fluctuations, estimated the adsorbed amount of surfactant molecules, and revealed multilayer coverage at high concentrations. The desorption rate of surfactant (18.4 s−1) during the microscopic motion of oil droplets was determined by in situ differentiating between droplet visited and non-visited areas. LA - English DB - MTMT ER - TY - GEN AU - Borbély, Krisztina AU - Balogh, Dóra Anett AU - Kurunczi, Sándor AU - Farkas, Enikő AU - Péter, Beatrix AU - Székács, Inna AU - Endre, Gabriella AU - Kondorosi, Eva AU - Horváth, Róbert TI - From protein-peptide interactions to cell adhesion: label-free biophysical insights into peptide activity PY - 2025 UR - https://m2.mtmt.hu/api/publication/36333981 ID - 36333981 LA - English DB - MTMT ER - TY - GEN AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, Istvan AU - Kitahata, Hiroyuki AU - J. Suematsu, Nobuhiko AU - Horváth, Róbert TI - Self-Propelled Droplet Motion with Characterization of Real-Time Kinetic Analysis of Surfactant Interactions by Label-Free Surface-Sensitive Biosensors PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334145 ID - 36334145 LA - English DB - MTMT ER - TY - GEN AU - Farkas, Enikő AU - Kovács, Kinga Dóra AU - Székács, Inna AU - Péter, Beatrix AU - Lagzi, Istvan AU - Kitahata, Hiroyuki AU - J. Suematsu, Nobuhiko AU - Horváth, Róbert TI - Real time, Nanometer Scale Resolution Kinetic Detection of Self Propelled Droplet Motion by Waveguide Biosensor PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334149 ID - 36334149 LA - English DB - MTMT ER - TY - GEN AU - Balogh, Dóra Anett AU - Novák, Szabolcs AU - Farkas, Enikő AU - Péter, Beatrix AU - Domokos, Heléna Brigitta AU - Szabó, Bálint AU - Horváth, Róbert TI - Technical advancements in circulating tumor cell sorting using a piezoelectric micropipette PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334158 ID - 36334158 LA - English DB - MTMT ER - TY - GEN AU - Borbély, Krisztina AU - Balogh, Dóra Anett AU - Kurunczi, Sándor AU - Farkas, Enikő AU - Péter, Beatrix AU - Székács, Inna AU - Endre, Gabriella AU - Kondorosi, Eva AU - Horváth, Róbert TI - GCI-based profiling of protein-peptide interactions PY - 2025 UR - https://m2.mtmt.hu/api/publication/36435130 ID - 36435130 LA - English DB - MTMT ER - TY - GEN AU - Bányai, Anita AU - Farkas, Enikő AU - Jankovics, Hajnalka AU - Székács, Inna AU - Leelőssyné Tóth, Eszter AU - Vonderviszt, Ferenc AU - Horváth, Róbert AU - Varga, Máté AU - Fürjes, Péter TI - Cells and Model Particles in Lateral Focusing Microfluidics PY - 2024 UR - https://m2.mtmt.hu/api/publication/35672592 ID - 35672592 LA - English DB - MTMT ER - TY - GEN AU - Magyaródi, Beatrix AU - Kovács, Kinga Dóra AU - Kovács, Boglárka AU - Farkas, Enikő AU - Székács, Inna AU - Vonderviszt, Ferenc AU - Horváth, Róbert TI - Applications of label-free biophysical methods in studying living cells PY - 2024 UR - https://m2.mtmt.hu/api/publication/35672976 ID - 35672976 LA - English DB - MTMT ER - TY - GEN AU - Székács, Inna AU - Farkas, Enikő AU - Kovács, Boglárka AU - Takács, Eszter AU - Oláh (Ottucsák), Marianna AU - Székács, András AU - Horváth, Róbert TI - Discovery of cell adhesion-modifying effects of glyphosate on living cells by label-free optical biosensing PY - 2024 UR - https://m2.mtmt.hu/api/publication/35674490 ID - 35674490 LA - English DB - MTMT ER - TY - JOUR AU - Bányai, Anita AU - Farkas, Enikő AU - Jankovics, Hajnalka AU - Székács, Inna AU - Leelőssyné Tóth, Eszter AU - Vonderviszt, Ferenc AU - Horváth, Róbert AU - Varga, Máté AU - Fürjes, Péter TI - Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels JF - SENSORS J2 - SENSORS-BASEL VL - 23 PY - 2023 IS - 2 PG - 19 SN - 1424-8220 DO - 10.3390/s23020800 UR - https://m2.mtmt.hu/api/publication/33552859 ID - 33552859 AB - The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli (E. coli), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae, and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel. LA - English DB - MTMT ER -