TY - GEN AU - Kovács, Kinga Dóra AU - Visnovitz, Tamás AU - Gerecsei, Tamás AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Koncz, Anna AU - Németh, Krisztina AU - Lenzinger, Dorina AU - Vukman, Krisztina V AU - Balogh, Anna AU - Rajmon, Imola AU - Lőrincz, Péter AU - Székács, Inna AU - Buzás, Edit I AU - Horváth, Róbert TI - Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy PY - 2025 UR - https://m2.mtmt.hu/api/publication/36334172 ID - 36334172 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Kinga Dóra AU - Visnovitz, Tamás AU - Gerecsei, Tamás AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Koncz, Anna AU - Németh, Krisztina AU - Lenzinger, Dorina AU - Visnovitzné Dr Vukman, Krisztina AU - Balogh, Anna AU - Rajmon, Imola AU - Lőrincz, Péter AU - Székács, Inna AU - Buzás, Edit Irén AU - Horváth, Róbert TI - Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy JF - JOURNAL OF EXTRACELLULAR VESICLES J2 - J EXTRACELLULAR VESICL VL - 12 PY - 2023 IS - 12 PG - 16 SN - 2001-3078 DO - 10.1002/jev2.12388 UR - https://m2.mtmt.hu/api/publication/34425078 ID - 34425078 AB - In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Bálint AU - Salánki, Rita Zsanett AU - Francz, Barbara AU - Gerecsei, Tamás AU - Horváth, Róbert TI - Egyedi sejtek manipulációi robotizált mikropipettával JF - FIZIKAI SZEMLE J2 - FIZIKAI SZEMLE VL - 73 PY - 2023 IS - 9 SP - 303 EP - 305 PG - 3 SN - 0015-3257 UR - https://m2.mtmt.hu/api/publication/34481960 ID - 34481960 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Kovács, Kinga Dóra AU - Visnovitz, Tamás AU - Gerecsei, Tamás AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Koncz, Anna AU - Németh, Krisztina AU - Lenzinger, Dorina AU - V. Vukman, Krisztina AU - Lőrincz, Péter AU - Székács, Inna AU - Buzás, Edit I. AU - Horváth, Róbert ED - Derényi, Imre ED - László, Grama ED - Solymosi, Katalin TI - Nanoinjection of fluorescent nanoparticles to single live cells by robotic fluidic force microscopy T2 - Magyar Biofizikai Társaság XXIX. Kongresszusa PB - Magyar Biofizikai Társaság CY - Budapest SN - 9786150186887 PY - 2023 SP - 35 EP - 35 PG - 1 UR - https://m2.mtmt.hu/api/publication/34486404 ID - 34486404 LA - English DB - MTMT ER - TY - GEN AU - Kovács, Kinga Dóra AU - Visnovitz, Tamás AU - Kanyó, Nicolett AU - Gerecsei, Tamás AU - Péter, Beatrix AU - Lagzi, István AU - Kurunczi, Sándor AU - Koncz, Anna AU - Németh, Krisztina AU - Lenzinger, Dorina AU - V. Vukman, Krisztina AU - Molnár, Kinga AU - Truszka, Mónika AU - Nakanishi, Hideyuki AU - Lőrincz, Péter AU - Székács, Inna AU - Buzás, Edit I. AU - Horváth, Róbert TI - Nanoinjection of fluorescent and gold nanoparticles to single live cells by robotic fluidic force microscopy PY - 2023 UR - https://m2.mtmt.hu/api/publication/34486423 ID - 34486423 LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Enikő AU - Tarr, Robert AU - Gerecsei, Tamás AU - Saftics, András AU - Kovács, Kinga Dóra AU - Stercz, Balázs AU - Domokos, Judit AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Bonyár, Attila AU - Bányai, Anita AU - Fürjes, Péter AU - Ruszkai-Szaniszló, Szilvia AU - Varga, Máté AU - Szabó, Barnabás AU - Ostorházi, Eszter AU - Szabó, Dóra AU - Horváth, Róbert TI - Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing JF - BIOSENSORS J2 - BIOSENSORS-BASEL VL - 12 PY - 2022 IS - 2 PG - 20 SN - 2079-6374 DO - 10.3390/bios12020056 UR - https://m2.mtmt.hu/api/publication/32621092 ID - 32621092 AB - Bacteria repellent surfaces and antibody-based coatings for bacterial assays have shown a growing demand in the field of biosensors, and have crucial importance in the design of biomedical devices. However, in-depth investigations and comparisons of possible solutions are still missing. The optical waveguide lightmode spectroscopy (OWLS) technique offers label-free, non-invasive, in situ characterization of protein and bacterial adsorption. Moreover, it has excellent flexibility for testing various surface coatings. Here, we describe an OWLS-based method supporting the development of bacteria repellent surfaces and characterize the layer structures and affinities of different antibody-based coatings for bacterial assays. In order to test nonspecific binding blocking agents against bacteria, OWLS chips were coated with bovine serum albumin (BSA), I-block, PAcrAM-g-(PMOXA, NH2, Si), (PAcrAM-P) and PLL-g-PEG (PP) (with different coating temperatures), and subsequent Escherichia coli adhesion was monitored. We found that the best performing blocking agents could inhibit bacterial adhesion from samples with bacteria concentrations of up to 107 cells/mL. Various immobilization methods were applied to graft a wide range of selected antibodies onto the biosensor’s surface. Simple physisorption, Mix&Go (AnteoBind) (MG) films, covalently immobilized protein A and avidin–biotin based surface chemistries were all fabricated and tested. The surface adsorbed mass densities of deposited antibodies were determined, and the biosensor;s kinetic data were evaluated to divine the possible orientations of the bacteria-capturing antibodies and determine the rate constants and footprints of the binding events. The development of affinity layers was supported by enzyme-linked immunosorbent assay (ELISA) measurements in order to test the bacteria binding capabilities of the antibodies. The best performance in the biosensor measurements was achieved by employing a polyclonal antibody in combination with protein A-based immobilization and PAcrAM-P blocking of nonspecific binding. Using this setting, a surface sensitivity of 70 cells/mm2 was demonstrated. LA - English DB - MTMT ER - TY - JOUR AU - Gerecsei, Tamás AU - Salánki, Rita Zsanett AU - Saftics, András AU - Derényi, Imre AU - Horváth, Róbert AU - Szabó, Bálint TI - Characterization of the Dissolution of Water Microdroplets in Oil JF - COLLOIDS AND INTERFACES J2 - COLLOID INTERFAC VL - 6 PY - 2022 IS - 1 PG - 13 SN - 2504-5377 DO - 10.3390/colloids6010014 UR - https://m2.mtmt.hu/api/publication/32709421 ID - 32709421 N1 - Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Office [PD 124559, KKP 129936]; ERC-HU; "Lendulet" Program of the Hungarian Academy of Sciences Funding text: This research was funded by the Hungarian National Research, Development and Innovation Office (grant numbers: PD 124559 for R.U.-S., KH-17, KKP 129936 and ERC-HU for R.H.), and the "Lendulet" Program of the Hungarian Academy of Sciences for R.H. AB - Water in oil emulsions have a wide range of applications from chemical technology to microfluidics, where the stability of water droplets is of paramount importance. Here, using an accessible and easily reproducible experimental setup we describe and characterize the dissolution of water in oil, which renders nanoliter-sized droplets unstable, resulting in their shrinkage and disappearance in a time scale of hours. This process has applicability in creating miniature reactors for crystallization. We test multiple oils and their combinations with surfactants exhibiting widely different rates of dissolution. We derived simple analytical equations to determine the product of the diffusion coefficient and the relative saturation density of water in oil from the measured dissolution data. By measuring the moisture content of mineral and silicone oils with Karl Fischer titration before and after saturating them with water, we calculated the diffusion coefficient of water in these two oils. LA - English DB - MTMT ER - TY - CHAP AU - Gerecsei, Tamás AU - Péter, Beatrix AU - Salánki, Rita Zsanett AU - Kurunczi, Sándor AU - Székács, Inna AU - Szabó, Bálint AU - Horváth, Róbert TI - Prospects of fluidic force microscopy and related biosensors for medical applications. Chapter 1 TS - Chapter 1 T2 - Nanobioanalytical Approaches to Medical Diagnostics PB - Elsevier CY - Cambridge, Massachusetts SN - 9780323851473 PY - 2022 SP - 1 EP - 28 PG - 28 DO - 10.1016/B978-0-323-85147-3.00014-1 UR - https://m2.mtmt.hu/api/publication/33098808 ID - 33098808 LA - English DB - MTMT ER - TY - GEN AU - Farkas, Enikő AU - Tarr, Robert AU - Gerecsei, Tamás AU - Saftics, András AU - Kovács, Kinga Dóra AU - Stercz, Balázs AU - Domokos, Judit AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Székács, Inna AU - Bonyár, Attila AU - Bányai, Anita AU - Fürjes, Péter AU - Ruszkai-Szaniszló, Szilvia AU - Varga, Máté AU - Szabó, Barnabás AU - Ostorházi, Eszter AU - Szabó, Dóra AU - Horváth, Róbert TI - Label-free optical biosensor method for detailed analysis of bacteria repellent and adhesive surfaces PY - 2022 UR - https://m2.mtmt.hu/api/publication/33548127 ID - 33548127 LA - English DB - MTMT ER - TY - GEN AU - Gerecsei, Tamás AU - Visnovitz, Tamás AU - Kovács, Kinga Dóra AU - Péter, Beatrix AU - Kurunczi, Sándor AU - Koncz, Anna AU - Németh, Krisztina AU - Lenzinger, Dorina AU - Visnovitzné Dr Vukman, Krisztina AU - Lőrincz, Péter AU - Székács, Inna AU - Buzás, Edit Irén AU - Horváth, Róbert TI - Nanoinjection of fluorescent nanoparticles to single live cells by robotic fluidic force microscopy CY - Poszter PY - 2022 UR - https://m2.mtmt.hu/api/publication/33589125 ID - 33589125 LA - English DB - MTMT ER -