TY - JOUR AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Imre, Norbert AU - Nath Bhaumik, Kaushik AU - Tököli, Attila AU - Füzesi, Tamás AU - Hollandi, Réka AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Deli, Mária Anna AU - Martinek, Tamás TI - α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 14 PY - 2022 IS - 3 PG - 11 SN - 1999-4923 DO - 10.3390/pharmaceutics14030580 UR - https://m2.mtmt.hu/api/publication/32733913 ID - 32733913 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.2.1-15-2016-00007]; Hungarian Ministry of Innovation and Technology [TUDFO/47138-1/2019-ITM]; Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG); Hungarian Academy of Sciences LENDULET-FoldamerHungarian Academy of Sciences; NKFINational Research, Development & Innovation Office (NRDIO) - Hungary [K134754]; Finnish TEKES FiDiPro Fellow Grant [40294/13]; Hungarian Academy of Sciences LENDULET-BiomagHungarian Academy of Sciences; Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund [TKP2021-EGA-32] Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and the Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer, and NKFI K134754. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, and the Hungarian Academy of Sciences LENDULET-Biomag. Support by the Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund (TKP2021-EGA-32) is acknowledged. AB - Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short alpha/beta-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform. LA - English DB - MTMT ER - TY - JOUR AU - Hetényi, Anasztázia AU - Imre, Norbert AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Fehérje méretű molekulák humán sejtekbe juttatása lipid-raft mediált endocitózissal JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 45 PY - 2021 IS - 4 SP - 67 EP - 83 PG - 17 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32570862 ID - 32570862 N1 - Nincs jelölve levelező szerzőség a közleményen. (BÉ SZTE admin5) LA - Hungarian DB - MTMT ER - TY - THES AU - Imre, Norbert TI - Intracellular protein delivery with the use of endocytosis routing sequences [Fehérjék sejtbe juttatása endocitózis útvonalra irányító szekvenciákkal] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 53 DO - 10.14232/phd.10645 UR - https://m2.mtmt.hu/api/publication/32476788 ID - 32476788 LA - English DB - MTMT ER - TY - PAT AU - Imre, Norbert AU - Martinek, Tamás AU - Hetényi, Anasztázia AU - Bodnár, Brigitta AU - Monostori, Eva AU - Czibula, Agnes AU - Szabo, Eniko AU - Deli, Mária Anna AU - Horvath, Peter TI - Endocytosis routing sequence peptide for cell delivery systems PY - 2020 UR - https://m2.mtmt.hu/api/publication/32024174 ID - 32024174 AB - The present invention relates to cell delivery systems. The present invention specifically relates to new methods of intracellular delivery by endocytosis routing sequence peptides having the sequence of WYKYV or analogs thereof, by caveloar/lipid raft-mediated endocytosis and uses of such peptides. The invention also relates to conjugates comprising said peptides and uses thereof in therapies wherein intracellular delivery of a therapeutically active mol. is required. LA - English DB - MTMT ER - TY - JOUR AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Routing Nanomolar Protein Cargoes to Lipid Raft‐Mediated/Caveolar Endocytosis through a Ganglioside GM1‐Specific Recognition Tag JF - ADVANCED SCIENCE J2 - ADV SCI VL - 7 PY - 2020 IS - 4 PG - 10 SN - 2198-3844 DO - 10.1002/advs.201902621 UR - https://m2.mtmt.hu/api/publication/31126947 ID - 31126947 N1 - Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, the Hungarian Academy of Sciences LENDULET-Biomag. LA - English DB - MTMT ER - TY - GEN AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, A. Mária AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - IgG bejuttatása specifikus GM1 gangliozid felismerő szekvenciával PY - 2019 UR - https://m2.mtmt.hu/api/publication/31624665 ID - 31624665 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ismail, Ruba AU - Sovány, Tamás AU - Gácsi, Attila AU - Ambrus, Rita AU - Katona, Gábor AU - Imre, Norbert AU - Pannonhalminé Csóka, Ildikó TI - Synthesis and Statistical Optimization of Poly (Lactic-Co-Glycolic Acid) Nanoparticles Encapsulating GLP1 Analog Designed for Oral Delivery JF - PHARMACEUTICAL RESEARCH J2 - PHAR RES VL - 36 PY - 2019 IS - 7 PG - 16 SN - 0724-8741 DO - 10.1007/s11095-019-2620-9 UR - https://m2.mtmt.hu/api/publication/30679490 ID - 30679490 N1 - University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Szeged, H-6720, Hungary University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Analysis, Somogyi u. 4, Szeged, H-6720, Hungary Export Date: 15 November 2019 CODEN: PHREE Correspondence Address: Csóka, I.; University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Hungary; email: csoka@pharm.u-szeged.hu Chemicals/CAS: chlormethine, 51-75-2, 55-86-7, 82905-71-3; liraglutide, 204656-20-2; polyglactin, 26780-50-7, 34346-01-5; Drug Carriers; Emulsions; Hypoglycemic Agents; Liraglutide; Polylactic Acid-Polyglycolic Acid Copolymer Manufacturers: xi an health, China Funding details: EFOP-3.6.1-16-2016-00008 Funding text 1: This research was supported by the EU-funded Hungarian grant EFOP-3.6.1-16-2016-00008. University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Szeged, H-6720, Hungary University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Analysis, Somogyi u. 4, Szeged, H-6720, Hungary Cited By :1 Export Date: 22 November 2019 CODEN: PHREE Correspondence Address: Csóka, I.; University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Hungary; email: csoka@pharm.u-szeged.hu Chemicals/CAS: chlormethine, 51-75-2, 55-86-7, 82905-71-3; liraglutide, 204656-20-2; polyglactin, 26780-50-7, 34346-01-5; Drug Carriers; Emulsions; Hypoglycemic Agents; Liraglutide; Polylactic Acid-Polyglycolic Acid Copolymer Manufacturers: xi an health, China Funding details: EFOP-3.6.1-16-2016-00008 Funding text 1: This research was supported by the EU-funded Hungarian grant EFOP-3.6.1-16-2016-00008. University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Szeged, H-6720, Hungary University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Analysis, Somogyi u. 4, Szeged, H-6720, Hungary Cited By :1 Export Date: 23 November 2019 CODEN: PHREE Correspondence Address: Csóka, I.; University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Hungary; email: csoka@pharm.u-szeged.hu Chemicals/CAS: chlormethine, 51-75-2, 55-86-7, 82905-71-3; liraglutide, 204656-20-2; polyglactin, 26780-50-7, 34346-01-5; Drug Carriers; Emulsions; Hypoglycemic Agents; Liraglutide; Polylactic Acid-Polyglycolic Acid Copolymer Manufacturers: xi an health, China Funding details: EFOP-3.6.1-16-2016-00008 Funding text 1: This research was supported by the EU-funded Hungarian grant EFOP-3.6.1-16-2016-00008. LA - English DB - MTMT ER - TY - JOUR AU - Bocsik, Alexandra AU - Gróf, Ilona AU - Kiss, Lóránd AU - Ötvös, Ferenc AU - Zsíros, Ottó AU - Daruka, Lejla AU - Fülöp, Lívia AU - Vastag, Monika AU - Kittel, Ágnes AU - Imre, Norbert AU - Martinek, Tamás AU - Pál, Csaba AU - Révész, Piroska AU - Deli, Mária Anna TI - Dual Action of the PN159/KLAL/MAP Peptide. Increase of Drug Penetration across Caco-2 Intestinal Barrier Model by Modulation of Tight Junctions and Plasma Membrane Permeability. TS - Increase of Drug Penetration across Caco-2 Intestinal Barrier Model by Modulation of Tight Junctions and Plasma Membrane Permeability. JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 11 PY - 2019 IS - 2 PG - 21 SN - 1999-4923 DO - 10.3390/pharmaceutics11020073 UR - https://m2.mtmt.hu/api/publication/30434125 ID - 30434125 AB - The absorption of drugs is limited by the epithelial barriers of the gastrointestinal tract. One of the strategies to improve drug delivery is the modulation of barrier function by the targeted opening of epithelial tight junctions. In our previous study the 18-mer amphiphilic PN159 peptide was found to be an effective tight junction modulator on intestinal epithelial and blood⁻brain barrier models. PN159, also known as KLAL or MAP, was described to interact with biological membranes as a cell-penetrating peptide. In the present work we demonstrated that the PN159 peptide as a penetration enhancer has a dual action on intestinal epithelial cells. The peptide safely and reversibly enhanced the permeability of Caco-2 monolayers by opening the intercellular junctions. The penetration of dextran molecules with different size and four efflux pump substrate drugs was increased several folds. We identified claudin-4 and -7 junctional proteins by docking studies as potential binding partners and targets of PN159 in the opening of the paracellular pathway. In addition to the tight junction modulator action, the peptide showed cell membrane permeabilizing and antimicrobial effects. This dual action is not general for cell-penetrating peptides (CPPs), since the other three CPPs tested did not show barrier opening effects. LA - English DB - MTMT ER - TY - JOUR AU - Hegedüs, Zsófia AU - Makra, Ildikó AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Mándity, István AU - Monostori, Éva AU - Martinek, Tamás TI - Foldameric probes for membrane interactions by induced β-sheet folding JF - CHEMICAL COMMUNICATIONS J2 - CHEM COMMUN VL - 52 PY - 2016 IS - 9 SP - 1891 EP - 1894 PG - 4 SN - 1359-7345 DO - 10.1039/C5CC09257D UR - https://m2.mtmt.hu/api/publication/2993079 ID - 2993079 N1 - Funding Agency and Grant Number: Hungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1]\n Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line.\n Funding Agency and Grant Number: Hungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1] Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line. Funding Agency and Grant Number: Hungarian Academy of SciencesHungarian Academy of Sciences; Lendulet program [LP-2011-009]; [TAMOP-4.2.6-14/1] Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet program (LP-2011-009) and TAMOP-4.2.6-14/1. Csaba Vizler is gratefully acknowledged for providing the bEND.3 cell line. AB - Design strategies were devised for alpha/beta-peptide foldameric analogues of the antiangiogenic anginex with the goal of mimicking the diverse structural features from the unordered conformation to a folded beta-sheet in response to membrane interactions. Structure-activity relationships were investigated in the light of different beta-sheet folding levels. LA - English DB - MTMT ER -