TY - JOUR AU - Poór, Miklós AU - Dombi, Ágnes AU - Fliszár-Nyúl, Eszter AU - Pedroni, Lorenzo AU - Dellafiora, Luca TI - Effects of Chrysin and Chrysin-7-sulfate on Ochratoxin A-Albumin Interactions and on the Plasma and Kidney Levels of the Mycotoxin in Rats JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 15 SP - 17655 EP - 17666 PG - 12 SN - 2470-1343 DO - 10.1021/acsomega.4c01738 UR - https://m2.mtmt.hu/api/publication/34766036 ID - 34766036 LA - English DB - MTMT ER - TY - JOUR AU - Fliszár-Nyúl, Eszter AU - Zinia Zaukuu, John-Lewis AU - Szente, Lajos AU - Kovács, Zoltán AU - Poór, Miklós TI - Impacts of β-cyclodextrin bead polymer (BBP) treatment on the quality of red and white wines: Color, polyphenol content, and electronic tongue analysis JF - LWT-FOOD SCIENCE AND TECHNOLOGY J2 - LWT-FOOD SCI TECHNOL VL - 176 PY - 2023 PG - 10 SN - 0023-6438 DO - 10.1016/j.lwt.2023.114567 UR - https://m2.mtmt.hu/api/publication/33633568 ID - 33633568 N1 - Export Date: 18 January 2024 CODEN: LBWTA LA - English DB - MTMT ER - TY - JOUR AU - Fliszár-Nyúl, Eszter AU - Ungvári, Orsolya AU - Dombi, Ágnes AU - Laczka, Csilla AU - Poór, Miklós TI - Interactions of Mycotoxin Alternariol with Cytochrome P450 Enzymes and OATP Transporters JF - METABOLITES J2 - METABOLITES VL - 13 PY - 2023 IS - 1 PG - 11 SN - 2218-1989 DO - 10.3390/metabo13010045 UR - https://m2.mtmt.hu/api/publication/33547988 ID - 33547988 N1 - * Megosztott szerzőség AB - Alternariol (AOH) is an emerging mycotoxin produced by Alternaria strains. The acute toxicity of the mycotoxin is low; however, chronic exposure to AOH may result in the development of endocrine disruptor and/or carcinogenic effects. The toxicokinetic properties of AOH have barely been characterized. Therefore, in this study, we aimed to investigate its interactions with CYP (1A2, 2C9, 2C19, 2D6, and 3A4) enzymes and OATP (1A2, 1B1, 1B3, and 2B1) transporters employing in vitro enzyme assays and OATP overexpressing cells, respectively. Our results demonstrated that AOH is a strong inhibitor of CYP1A2 (IC50 = 0.15 μM) and CYP2C9 (IC50 = 7.4 μM). Based on the AOH depletion assays in the presence of CYP enzymes, CYP1A2 is mainly involved, while CYP2C19 is moderately involved in the CYP-catalyzed biotransformation of the mycotoxin. AOH proved to be a strong inhibitor of each OATP transporter examined (IC50 = 1.9 to 5.4 μM). In addition, both direct and indirect assays suggest the involvement of OATP1B1 in the cellular uptake of the mycotoxin. These findings promote the deeper understanding of certain toxicokinetic interactions of AOH. LA - English DB - MTMT ER - TY - JOUR AU - Kaci, Hana AU - Bodnárová, Slávka AU - Fliszár-Nyúl, Eszter AU - Lemli, Beáta AU - Pelantová, Helena AU - Valentová, Kateřina AU - Bakos, Éva AU - Laczka, Csilla AU - Poór, Miklós TI - Interaction of luteolin, naringenin, and their sulfate and glucuronide conjugates with human serum albumin, cytochrome P450 (CYP2C9, CYP2C19, and CYP3A4) enzymes and organic anion transporting polypeptide (OATP1B1 and OATP2B1) transporters JF - BIOMEDICINE & PHARMACOTHERAPY J2 - BIOMED PHARMACOTHER VL - 157 PY - 2023 PG - 12 SN - 0753-3322 DO - 10.1016/j.biopha.2022.114078 UR - https://m2.mtmt.hu/api/publication/33294693 ID - 33294693 N1 - Drug Resistance Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar tudósok krt. 2, Budapest, H-1117, Hungary Doctoral School of Biology, Institute of Biology, Eötvös Loránd University, Pázmány P. stny. 1/C, Budapest, H-1117, Hungary Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus u. 2, Pécs, H-7624, Hungary Food Biotechnology Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, H-7624, Hungary Green Chemistry Research Group, János Szentágothai Research Center, University of Pécs, Ifjúság útja 20, Pécs, H-7624, Hungary Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, Prague, CZ-142, Czech Republic Cited By :1 Export Date: 4 September 2023 CODEN: BIPHE Correspondence Address: Poór, M.; Department of Pharmacology, Rókus u. 2, Hungary; email: poor.miklos@pte.hu AB - Luteolin and naringenin are flavonoids found in various foods/beverages and present in certain dietary supplements. After a high intake of these flavonoids, their sulfate and glucuronide conjugates reach micromolar concentrations in the bloodstream. Some pharmacokinetic interactions of luteolin and naringenin have been investigated in previous studies; however, only limited data are available in regard to their metabolites. In this study, we aimed to investigate the interactions of the sulfate and glucuronic acid conjugates of luteolin and naringenin with human serum albumin, cytochrome P450 (CYP2C9, 2C19, and 3A4) enzymes, and organic anion transporting polypeptide (OATP1B1 and OATP2B1) transporters. Our main findings are as follows: (1) Sulfate conjugates formed more stable complexes with albumin than the parent flavonoids. (2) Luteolin and naringenin conjugates showed no or only weak inhibitory action on the CYP enzymes examined. (3) Certain conjugates of luteolin and naringenin are potent inhibitors of OATP1B1 and/or OATP2B1 enzymes. (4) Conjugated metabolites of luteolin and naringenin may play an important role in the pharmacokinetic interactions of these flavonoids. LA - English DB - MTMT ER - TY - JOUR AU - Lemli, Beáta AU - Vilmányi, Péter AU - Fliszár-Nyúl, Eszter AU - Zsidó, Balázs Zoltán AU - Hetényi, Csaba AU - Szente, Lajos AU - Poór, Miklós TI - Testing Serum Albumins and Cyclodextrins as Potential Binders of the Mycotoxin Metabolites Alternariol-3-Sulfate, Alternariol-9-Monomethylether and Alternariol-9-Monomethylether-3-Sulfate JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 22 PG - 16 SN - 1661-6596 DO - 10.3390/ijms232214353 UR - https://m2.mtmt.hu/api/publication/33258382 ID - 33258382 N1 - Funding Agency and Grant Number: Hungarian National Research, Development, and Innovation Office [FK138184]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; Thematic Excellence Program 2020-National Excellence Sub-program of the Ministry for Innovation and Technology in Hungary; Medical School, University of Pecs [PTE AOK-KA 2021/KA-2021-39, KA-2022-26]; European Union [EFOP-3.6.1-16-2016-00004]; European Social Fund [EFOP-3.6.1-16-2016-00004] Funding text: M.P. is thankful for the support of the Hungarian National Research, Development, and Innovation Office (FK138184) and the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. The research was financed by the Thematic Excellence Program 2020-National Excellence Sub-program of the Ministry for Innovation and Technology in Hungary. The work was supported by the Medical School, University of Pecs, PTE AOK-KA 2021/KA-2021-39 and KA-2022-26. The project was supported by the European Union, and co-financed by the European Social Fund, project name and code: Comprehensive Development for Implementing Smart Specialization Strategies at the University of Pecs, EFOP-3.6.1-16-2016-00004. AB - Alternaria mycotoxins, including alternariol (AOH), alternariol-9-monomethylether (AME), and their masked/modified derivatives (e.g., sulfates or glycosides), are common food contaminants. Their acute toxicity is relatively low, while chronic exposure can lead to the development of adverse health effects. Masked/modified metabolites can probably release the more toxic parent mycotoxin due to their enzymatic hydrolysis in the intestines. Previously, we demonstrated the complex formation of AOH with serum albumins and cyclodextrins; these interactions were successfully applied for the extraction of AOH from aqueous matrices (including beverages). Therefore, in this study, the interactions of AME, alternariol-3-sulfate (AS), and alternariol-9-monomethylether-3-sulfate (AMS) were investigated with albumins (human, bovine, porcine, and rat) and with cyclodextrins (sulfobutylether-β-cyclodextrin, sugammadex, and cyclodextrin bead polymers). Our major results/conclusions are the following: (1) The stability of mycotoxin–albumin complexes showed only minor species dependent variations. (2) AS and AMS formed highly stable complexes with albumins in a wide pH range, while AME–albumin interactions preferred alkaline conditions. (3) AME formed more stable complexes with the cyclodextrins examined than AS and AMS. (4) Beta-cyclodextrin bead polymer proved to be highly suitable for the extraction of AME, AS, and AMS from aqueous solution. (5) Albumins and cyclodextrins are promising binders of the mycotoxins tested. LA - English DB - MTMT ER - TY - JOUR AU - Szőke, Zsuzsanna AU - Babarczi, Bianka AU - Mézes, Miklós AU - Lakatos, István AU - Poór, Miklós AU - Fliszár-Nyúl, Eszter AU - Oldal, Miklós AU - Czéh, Árpád AU - Bodó, Kornélia AU - Nagyéri, György AU - Ferenczi, Szilamér TI - Analysis and Comparison of Rapid Methods for the Determination of Ochratoxin A Levels in Organs and Body Fluids Obtained from Exposed Mice JF - TOXINS J2 - TOXINS VL - 14 PY - 2022 IS - 9 PG - 18 SN - 2072-6651 DO - 10.3390/toxins14090634 UR - https://m2.mtmt.hu/api/publication/33092073 ID - 33092073 AB - Mycotoxins are bioaccumulative contaminants impacting animals and humans. The simultaneous detection of frequent active exposures and accumulated mycotoxin level (s) in exposed organisms would be the most ideal to enable appropriate actions. However, few methods are available for the purpose, and there is a demand for dedicated, sensitive, reliable, and practical assays. To demonstrate the issue, mice were exposed to a relevant agent Ochratoxin A (OTA), and accumulated OTA was measured by fine-tuned commercial assays. Quantitative high-performance liquid chromatography with fluorescence detection, enzyme-linked immunosorbent assay, and flow cytometry assays have been developed/modified using reagents available as commercial products when appropriate. Assays were performed on excised samples, and results were compared. Accumulated OTA could be detected and quantified; positive correlations (between applied doses of exposure and accumulated OTA levels and the results from assays) were found. Dedicated assays could be developed, which provided comparable results. The presence and accumulation of OTA following even a short exposure could be quantitatively detected. The assays performed similarly, but HPLC had the greatest sensitivity. Blood contained higher levels of OTA than liver and kidney. We demonstrate that specific but flexible and practical assays should be used for specific/local purposes, to measure the exposure itself and accumulation in blood or organs. LA - English DB - MTMT ER - TY - JOUR AU - Fliszár-Nyúl, Eszter AU - Jakabfi-Csepregi, Rita AU - Benkovics, Gábor AU - Szente, Lajos AU - Poór, Miklós TI - Testing the Protective Effects of Sulfobutylether-Βeta-Cyclodextrin (SBECD) and Sugammadex against Chlorpromazine-Induced Acute Toxicity in SH-SY5Y Cell Line and in NMRI Mice JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 14 PY - 2022 IS - 9 PG - 13 SN - 1999-4923 DO - 10.3390/pharmaceutics14091888 UR - https://m2.mtmt.hu/api/publication/33088493 ID - 33088493 N1 - Funding Agency and Grant Number: New National Excellence Programof the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund [UNKP-21-5] Funding text: Supported by the UNKP-21-5 New National Excellence Programof the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. AB - Chlorpromazine (CPZ) is an antipsychotic drug which can cause several adverse effects and drug poisoning. Recent studies demonstrated that CPZ forms highly stable complexes with certain cyclodextrins (CDs) such as sulfobutylether-β-CD (SBECD) and sugammadex (SGD). Since there is no available antidote in CPZ intoxication, and considering the good tolerability of these CDs even if when administered parenterally, we aimed to investigate the protective effects of SBECD and SGD against CPZ-induced acute toxicity employing in vitro (SH-SY5Y neuroblastoma cells) and in vivo (zebrafish embryo) models. Our major findings and conclusions are the following: (1) both SBECD and SGD strongly relieved the cytotoxic effects of CPZ in SH-SY5Y cells. (2) SGD co-treatment did not affect or increase the CPZ-induced 24 h mortality in NMRI mice, while SBECD caused a protective effect in a dose-dependent fashion. (3) The binding constants of ligand–CD complexes and/or the in vitro protective effects of CDs can help to estimate the in vivo suitability of CDs as antidotes; however, some other factors can overwrite these predictions. LA - English DB - MTMT ER - TY - JOUR AU - Fliszár-Nyúl, Eszter AU - Bock, Illés AU - Jakabfi-Csepregi, Rita AU - Szente, Lajos AU - Szabó, István AU - Csenki, Zsolt Imre AU - Poór, Miklós TI - Testing the protective effects of cyclodextrins vs. alternariol-induced acute toxicity in HeLa cells and in zebrafish embryos JF - ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY J2 - ENVIRON TOXICOL PHAR VL - 95 PY - 2022 PG - 9 SN - 1382-6689 DO - 10.1016/j.etap.2022.103965 UR - https://m2.mtmt.hu/api/publication/33069606 ID - 33069606 N1 - Export Date: 18 January 2024 CODEN: ETOPF LA - English DB - MTMT ER - TY - JOUR AU - Fliszár-Nyúl, Eszter AU - Faisal, Anna Zelma AU - Skaper, Renáta AU - Lemli, Beáta AU - Bayartsetseg, Bayarsaikhan AU - Hetényi, Csaba AU - Gömbös, Patrik AU - Szabó, András AU - Poór, Miklós TI - Interaction of the Emerging Mycotoxins Beauvericin, Cyclopiazonic Acid, and Sterigmatocystin with Human Serum Albumin JF - BIOMOLECULES J2 - BIOMOLECULES VL - 12 PY - 2022 IS - 8 PG - 11 SN - 2218-273X DO - 10.3390/biom12081106 UR - https://m2.mtmt.hu/api/publication/33050125 ID - 33050125 N1 - Export Date: 18 January 2024 AB - Beauvericin (BEA), cyclopiazonic acid (CPA), and sterigmatocystin (STC) are emerging mycotoxins. They appear as contaminants in food and animal feed, leading to economic losses and health risks. Human serum albumin (HSA) forms stable complexes with certain mycotoxins, including ochratoxins, alternariol, citrinin, and zearalenone. HSA binding can influence the toxicokinetics of xenobiotics, and albumin can also be considered and applied as a relatively cheap affinity protein. Therefore, we examined the potential interactions of BEA, CPA, and STC with HSA employing fluorescence spectroscopy, ultracentrifugation, ultrafiltration, and molecular modeling. Spectroscopic and ultracentrifugation studies demonstrated the formation of low-affinity BEA–HSA (Ka ≈ 103 L/mol) and moderately strong CPA–HSA and STC–HSA complexes (Ka ≈ 104 L/mol). In ultrafiltration experiments, CPA slightly displaced each site marker (warfarin, naproxen, and camptothecin) tested, while BEA and STC did not affect significantly the albumin binding of these drugs. Modeling studies suggest that CPA occupies Sudlow’s site I, while STC binds to the Heme site (FA1) on HSA. Considering the interactions of CPA with the site markers, the CPA–HSA interaction may have toxicological importance. LA - English DB - MTMT ER - TY - JOUR AU - Pásztiné Gere, Erzsébet AU - Szentkirályi-Tóth, Anna AU - Szabó, Pál Tamás AU - Steinmetzer, Torsten AU - Fliszár-Nyúl, Eszter AU - Poór, Miklós TI - In vitro characterization of the furin inhibitor MI-1851: Albumin binding, interaction with cytochrome P450 enzymes and cytotoxicity JF - BIOMEDICINE & PHARMACOTHERAPY J2 - BIOMED PHARMACOTHER VL - 151 PY - 2022 PG - 8 SN - 0753-3322 DO - 10.1016/j.biopha.2022.113124 UR - https://m2.mtmt.hu/api/publication/32828207 ID - 32828207 N1 - Export Date: 18 January 2024 CODEN: BIPHE LA - English DB - MTMT ER -