TY - JOUR AU - Barabási, Beáta AU - Barna, Lilla AU - Santa Maria, Anaraquel AU - Harazin, András AU - Molnár, Réka AU - Kincses, András AU - Vigh, Judit Piroska AU - Dukay, Brigitta AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda AU - Walter, Fruzsina AU - Deli, Mária Anna AU - Hoyk, Zsófia TI - Role of interleukin-6 and interleukin-10 in morphological and functional changes of the blood–brain barrier in hypertriglyceridemia JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 20 PY - 2023 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-023-00418-3 UR - https://m2.mtmt.hu/api/publication/33688118 ID - 33688118 N1 - Funding Agency and Grant Number: ELKH Biological Research Center - National Research, Development, and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00060]; European Training Network [H2020-MSCA-ITN-2015, 675619] Funding text: Open access funding provided by ELKH Biological Research Center. This work was funded by the National Research, Development, and Innovation Office of Hungary, Grant Number GINOP-2.3.2-15-2016-00060. ARSM was supported by the European Training Network H2020-MSCA-ITN-2015 [Grant Number 675619]. LA - English DB - MTMT ER - TY - JOUR AU - Lee, Mei-Hwa AU - Jan, Jeng-Shiung AU - Thomas, James L. AU - Shih, Yuan-Pin AU - Li, Jin-An AU - Lin, Chien-Yu AU - Ooya, Tooru AU - Barna, Lilla AU - Mészáros, Mária AU - Harazin, András AU - Porkoláb, Gergő AU - Veszelka, Szilvia AU - Deli, Mária Anna AU - Lin, Hung-Yin TI - Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model JF - CELLS J2 - CELLS-BASEL VL - 11 PY - 2022 IS - 16 PG - 13 SN - 2073-4409 DO - 10.3390/cells11162584 UR - https://m2.mtmt.hu/api/publication/33064300 ID - 33064300 N1 - Funding Agency and Grant Number: Ministry of Science and Technology of ROC [MOST 107-2923-M-006-002-MY3, MOST 107-2923-M-390-001-MY3, MOST 108-2221-E-006-034-MY3, MOST 108-2923-B-390-001-MY3, MOST 110-2221-E-390-003-MY3]; National Research, Development and Innovation Office, Budapest, Hungary [PD138930, NNE29617]; Kobe University grant for promoting international joint research; Gedeon Richter Plc Centenarial Foundation [H-1103]; Premium Postdoctoral Research Program of the Hungarian Academy of Sciences [Premium-2019-469]; Szeged Scientists Academy of the Hungarian Ministry of Innovation and Technology [FEIF/646-4/2021-ITM_SZERZ]; New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund [UNKP-21-3-405] Funding text: The authors would like to give their appreciation to the Ministry of Science and Technology of ROC under Contract nos. MOST 107-2923-M-006-002-MY3, MOST 107-2923-M-390-001-MY3, MOST 108-2221-E-006-034-MY3, MOST 108-2923-B-390-001-MY3, MOST 109-2221-E-214-028-and MOST 110-2221-E-390-003-MY3. This work was supported by a research grant of the National Research, Development and Innovation Office, Budapest, Hungary (NNE29617 to M.A.D.) in the frame of the nanoPD consortium, and partially supported by the Kobe University grant for promoting international joint research (to TO). M.M. was supported by the research grant of the National Research, Development and Innovation Office, Budapest, Hungary (PD138930) and the Gedeon Richter Plc Centenarial Foundation (H-1103 Budapest, Gyomr oi str. 19-21. Hungary). S.V. was supported by the Premium Postdoctoral Research Program (Premium-2019-469) of the Hungarian Academy of Sciences. G.P. was supported by the Szeged Scientists Academy under the sponsorship of the Hungarian Ministry of Innovation and Technology (FEIF/646-4/2021-ITM_SZERZ), as well as by the UNKP-21-3-405 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. AB - Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs. LA - English DB - MTMT ER - TY - JOUR AU - Huber, Imre AU - Pandur, Edina AU - Sipos, Katalin AU - Barna, Lilla AU - Harazin, András AU - Deli, Mária Anna AU - Tyukodi, Levente AU - Gulyás, Gergely AU - Kulcsár, Győző AU - Rozmer, Zsuzsanna TI - Novel cyclic C5-curcuminoids penetrating the blood-brain barrier: design, synthesis and antiproliferative activity against astrocytoma and neuroblastoma cells JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 173 PY - 2022 PG - 29 SN - 0928-0987 DO - 10.1016/j.ejps.2022.106184 UR - https://m2.mtmt.hu/api/publication/32779076 ID - 32779076 AB - Novel series of cyclic C5-curcuminoids 17a-j and 19-22 were prepared as cytotoxic agents and evaluated against human neuroblastoma (SH-SY5Y) or human grade IV astrocytoma (CCF-STTG1) cell lines in low (∼0.1 nM - 10 nM) concentrations. Among the tested 21 derivatives, 16 displayed potent antiproliferative activity with IC50 values in the low nanomolar to picomolar range (IC50 = 7.483-0.139 nM). Highly active compounds like N-monocarboxylic derivative 19b with IC50 = 0.139 nM value against neuroblastoma and N-alkyl substituted 11 with IC50 = 0.257 nM against astrocytoma proved some degree of selectivity toward non-cancerous astrocytes and kidney cells. This potent anticancer activity did not show a strong correlation with experimental logPTLC values, but the most potent antiproliferative molecules 11-13 and 19-22 are belonging to discrete subgroups of the cyclic C5-curcuminoids. Compounds 12, 17c and 19b were subjected to blood-brain barrier (BBB) penetration studies, too. The BBB was revealed to be permeable for all of them but, as the apparent permeability coefficient (Papp) values mirrored, in different ratios. Lower toxicity of 12, 17c and 19b was observed toward primary rat brain endothelial cells of the BBB model, which means they remained undamaged under 10 µM concentrations. Penetration depends, at least in part, on albumin binding of 12, 17c and 19b and the presence of monocarboxylic acid transporters in the case of 19b. Permeation through the BBB and albumin binding, we described here, is the first example of cyclic C5-curcuminoids as to our knowledge. LA - English DB - MTMT ER - TY - JOUR AU - Walter, Fruzsina AU - Harazin, András AU - Tóth, Andrea AU - Veszelka, Szilvia AU - Santa Maria, Anaraquel AU - Barna, Lilla AU - Kincses, András AU - Biczo, G AU - Balla, Zsolt AU - Kui, Balázs AU - Maléth, József AU - Cervenak, László AU - Tubak, Vilmos AU - Kittel, Ágnes AU - Rakonczay, Zoltán AU - Deli, Mária Anna TI - Blood–brain barrier dysfunction in l-ornithine induced acute pancreatitis in rats and the direct effect of l-ornithine on cultured brain endothelial cells JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 19 PY - 2022 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-022-00308-0 UR - https://m2.mtmt.hu/api/publication/32667372 ID - 32667372 N1 - Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, Szeged, 6726, Hungary Department of Medicine, University of Szeged, Kálvária sgt 57, Szeged, 6725, Hungary Department of Pathophysiology, University of Szeged, Semmelweis u. 1, Szeged, 6701, Hungary HAS-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, University of Szeged, Dóm sqr. 10, Szeged, 6720, Hungary HCEMM-SZTE Molecular Gastroenterology Research Group, University of Szeged, Dóm sqr. 10, Szeged, 6720, Hungary Department of Internal Medicine and Hematology, Research Laboratory, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary Creative Laboratory Ltd, Temesvári krt. 62, Szeged, 6726, Hungary Institute of Experimental Medicine, Eötvös Loránd Research Network, Szigony u. 43, Budapest, 1083, Hungary Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 Blackfan Circle, Boston, MA 02115, United States Department of Biomedicine, Faculty of Health, Aarhus University, Høegh-Guldbergs Gade 10, Aarhus C, 8000, Denmark Institute of Applied Sciences, Department of Environmental Biology and Education, Juhász Gyula Faculty of Education, University of Szeged, Boldogasszony sgt. 6, Szeged, 6725, Hungary Cited By :1 Export Date: 23 November 2022 Correspondence Address: Deli, M.A.; Institute of Biophysics, Temesvári krt. 62, Hungary; email: deli.maria@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Váczi, Sándor AU - Barna, Lilla AU - Harazin, András AU - Mészáros, Mária AU - Porkoláb, Gergő AU - Zvara, Ágnes AU - Ónody, Rita AU - Földesi, Imre AU - Veszelka, Szilvia AU - Penke, Botond AU - Fülöp, Lívia AU - Deli, Mária Anna AU - Mezei, Zsófia TI - S1R agonist modulates rat platelet eicosanoid synthesis and aggregation JF - PLATELETS J2 - PLATELETS VL - 33 PY - 2022 IS - 5 SP - 709 EP - 718 PG - 10 SN - 0953-7104 DO - 10.1080/09537104.2021.1981843 UR - https://m2.mtmt.hu/api/publication/32491017 ID - 32491017 AB - Sigma-1 receptor (S1R) is detected in different cell types and can regulate intracellular signaling pathways. S1R plays a role in the pathomechanism of diseases and the regulation of neurotransmitters. Fluvoxamine can bind to S1R and reduce the serotonin uptake of neurons and platelets. We therefore hypothesized that platelets express S1R, which can modify platelet function. The expression of the SIGMAR1 gene in rat platelets was examined with a reverse transcription polymerase chain reaction and a quantitative polymerase chain reaction. The receptor was also visualized by immunostaining and confocal laser scanning microscopy. The effect of S1R agonist PRE-084 on the eicosanoid synthesis of isolated rat platelets and ADP- and AA-induced platelet aggregation was examined. S1R was detected in rat platelets both at gene and protein levels. Pretreatment with PRE-084 of resting platelets induced elevation of eicosanoid synthesis. The rate of elevation in thromboxane B2 and prostaglandin D2 synthesis was similar, but the production of prostaglandin E2 was higher. The concentration-response curve showed a sigmoidal form. The most effective concentration of the agonist was 2 µM. PRE-084 increased the quantity of cyclooxygenase-1 as detected by ELISA. PRE-084 also elevated the ADP- and AA-induced platelet aggregation. S1R of platelets might regulate physiological or pathological functions. © 2021 Taylor & Francis Group, LLC. LA - English DB - MTMT ER - TY - JOUR AU - Balla, Zsolt AU - Kormányos, Eszter Sára AU - Kui, Balázs AU - Bálint, Emese Réka AU - Fűr, Gabriella AU - Orján, Erik Márk AU - Iványi, Béla AU - Vécsei, László AU - Fülöp, Ferenc AU - Varga, Gabriella AU - Harazin, András AU - Tubak, Vilmos AU - Deli, Mária Anna AU - Papp, Csaba Gergő AU - Gácser, Attila AU - Madácsy, Tamara AU - Venglovecz, Viktória AU - Maléth, József AU - Hegyi, Péter AU - Kiss, Lóránd AU - Rakonczay, Zoltán TI - Kynurenic acid and its analogue SZR-72 ameliorate the severity of experimental acute necrotizing pancreatitis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 15 SN - 1664-3224 DO - 10.3389/fimmu.2021.702764 UR - https://m2.mtmt.hu/api/publication/32258744 ID - 32258744 LA - English DB - MTMT ER - TY - JOUR AU - Szczepkowska, Aleksandra AU - Harazin, András AU - Barna, Lilla AU - Deli, Mária Anna AU - Skipor, Janina TI - Identification of Reference Genes for Circadian Studies on Brain Microvessels and Choroid Plexus Samples Isolated from Rats JF - BIOMOLECULES J2 - BIOMOLECULES VL - 11 PY - 2021 IS - 8 PG - 18 SN - 2218-273X DO - 10.3390/biom11081227 UR - https://m2.mtmt.hu/api/publication/32166650 ID - 32166650 N1 - Funding Agency and Grant Number: KNOW Consortium"Healthy Animal -Safe Food", MSHE [05-1/KNOW2/2015]; Institute of Animals Reproduction and Food Research statutory research funds. LA - English DB - MTMT ER - TY - JOUR AU - L. Kiss, Eszter AU - Berkó, Szilvia AU - Gácsi, Attila AU - Kovács, Anita AU - Katona, Gábor AU - Soós, Judit AU - Csányi, Erzsébet AU - Gróf, Ilona AU - Harazin, András AU - Deli, Mária Anna AU - Balogh, György Tibor AU - Budai-Szűcs, Mária TI - Development and Characterization of Potential Ocular Mucoadhesive Nano Lipid Carriers Using Full Factorial Design JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 12 PY - 2020 IS - 7 PG - 23 SN - 1999-4923 DO - 10.3390/pharmaceutics12070682 UR - https://m2.mtmt.hu/api/publication/31385933 ID - 31385933 N1 - Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi Fasor 10-11, Szeged, H-6720, Hungary Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Doctoral School of Biology, University of Szeged, Dugonics tér 13, Szeged, H-6720, Hungary Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Műegyetem rakpart 3, Budapest, 1111, Hungary Cited By :11 Export Date: 1 March 2024 Correspondence Address: Budai-Szűcs, M.; Institute of Pharmaceutical Technology and Regulatory Affairs, Eötvös u. 6, Hungary; email: maria.szucs@pharm.u-szeged.hu Chemicals/CAS: cremophor, 39279-69-1, 51142-51-9; dexamethasone, 50-02-2; glycerol behenate, 18641-57-1; hydrogenated castor oil, 8001-78-3; hydroxypropylmethylcellulose, 9004-65-3; macrogol, 25322-68-3; miglyol, 37332-31-3, 77466-09-2, 77944-80-0, 97708-73-1; occludin, 176304-61-3; uvomorulin, 112956-45-3 LA - English DB - MTMT ER - TY - JOUR AU - Porkoláb, Gergő AU - Mészáros, Mária AU - Tóth, András AU - Szecskó, Anikó AU - Harazin, András AU - Szegletes, Zsolt AU - Ferenc, Györgyi AU - Blastyák, András AU - Mátés, Lajos AU - Rákhely, Gábor AU - Deli, Mária Anna AU - Veszelka, Szilvia TI - Combination of Alanine and Glutathione as Targeting Ligands of Nanoparticles Enhances Cargo Delivery into the Cells of the Neurovascular Unit JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 12 PY - 2020 IS - 7 PG - 23 SN - 1999-4923 DO - 10.3390/pharmaceutics12070635 UR - https://m2.mtmt.hu/api/publication/31377384 ID - 31377384 N1 - These authors contributed equally to this manuscript. (Porkoláb G and Mészáros M) LA - English DB - MTMT ER - TY - JOUR AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Harazin, András AU - Santa Maria, Anaraquel AU - Vizsnyiczai, Gaszton AU - Barna, Lilla AU - Kiss, Lóránd AU - Fűr, Gabriella AU - Rakonczay, Zoltán AU - Ambrus, Rita AU - Révész, Piroska AU - Gosselet, Fabien AU - Pongsiri, Jaikumpun AU - Szabó, Hajnalka AU - Zsembery, Ákos AU - Deli, Mária Anna TI - The Effect of Sodium Bicarbonate, a Beneficial Adjuvant Molecule in Cystic Fibrosis, on Bronchial Epithelial Cells Expressing a Wild-Type or Mutant CFTR Channel JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 11 PG - 23 SN - 1661-6596 DO - 10.3390/ijms21114024 UR - https://m2.mtmt.hu/api/publication/31335512 ID - 31335512 LA - English DB - MTMT ER -