TY - JOUR AU - Fabó, Dániel AU - Bokodi, Virág AU - Szabó, Johanna Petra AU - Tóth, Emília AU - Salami, Pariya AU - Keller, Corey J AU - Hajnal, Boglárka Zsófia AU - Thesen, Thomas AU - Devinsky, Orrin AU - Doyle, Werner AU - Mehta, Ashesh AU - Madsen, Joseph AU - Eskandar, Emad AU - Erőss, Loránd AU - Ulbert, István AU - Halgren, Eric AU - Cash, Sydney S TI - The role of superficial and deep layers in the generation of high frequency oscillations and interictal epileptiform discharges in the human cortex JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 17 SN - 2045-2322 DO - 10.1038/s41598-022-22497-2 UR - https://m2.mtmt.hu/api/publication/34019088 ID - 34019088 N1 - Epilepsy Unit, Department of Neurology, National Institute of Mental Health, Neurology and Neurosurgery, Amerikai Út 57. 1145, Budapest, Hungary Roska Tamás Doctoral School of Sciences and Technologies, Budapest, Hungary János Szentágothai Doctoral School of Neurosciences, Budapest, Hungary Department of Neurology, University of Texas, McGovern Medical School, Houston, TX, United States Epilepsy Division, Department of Neurology, Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States Department of Psychiatry and Behavioral Sciences, Stanford University Medical Center, Stanford, CA, United States VA Palo Alto Health Care System, Palo Alto, CA, United States Comprehensive Epilepsy Center, New York University School of Medicine, New York, NY, United States Department of Biomedical Sciences, College of Medicine, University of Houston, Houston, TX, United States Department of Neurosurgery, Zucker School of Medicine at Hofstra/Northwell and Feinstein Institute for Medical Research, Manhasset, NY, United States The Children’s Hospital, Boston, MA, United States Massachusetts General Hospital Neurosurgery Research, Boston, MA, United States Department of Functional Neurosurgery, National Institute of Mental Health, Neurology and Neurosurgery, Budapest, Hungary Institute of Psychology, Eötvös Loránd Research Network, Budapest, Hungary Department of Radiology, Neurosciences and Psychiatry, University of California, San Diego, San Diego, CA, United States Export Date: 30 October 2023 Correspondence Address: Fabo, D.; Epilepsy Unit, Amerikai Út 57. 1145, Hungary; email: fabo@oiti.hu Correspondence Address: Cash, S.S.; Epilepsy Division, United States; email: scash@mgh.harvard.edu AB - Describing intracortical laminar organization of interictal epileptiform discharges (IED) and high frequency oscillations (HFOs), also known as ripples. Defining the frequency limits of slow and fast ripples. We recorded potential gradients with laminar multielectrode arrays (LME) for current source density (CSD) and multi-unit activity (MUA) analysis of interictal epileptiform discharges IEDs and HFOs in the neocortex and mesial temporal lobe of focal epilepsy patients. IEDs were observed in 20/29, while ripples only in 9/29 patients. Ripples were all detected within the seizure onset zone (SOZ). Compared to hippocampal HFOs, neocortical ripples proved to be longer, lower in frequency and amplitude, and presented non-uniform cycles. A subset of ripples (≈ 50%) co-occurred with IEDs, while IEDs were shown to contain variable high-frequency activity, even below HFO detection threshold. The limit between slow and fast ripples was defined at 150 Hz, while IEDs' high frequency components form clusters separated at 185 Hz. CSD analysis of IEDs and ripples revealed an alternating sink-source pair in the supragranular cortical layers, although fast ripple CSD appeared lower and engaged a wider cortical domain than slow ripples MUA analysis suggested a possible role of infragranularly located neural populations in ripple and IED generation. Laminar distribution of peak frequencies derived from HFOs and IEDs, respectively, showed that supragranular layers were dominated by slower (< 150 Hz) components. Our findings suggest that cortical slow ripples are generated primarily in upper layers while fast ripples and associated MUA in deeper layers. The dissociation of macro- and microdomains suggests that microelectrode recordings may be more selective for SOZ-linked ripples. We found a complex interplay between neural activity in the neocortical laminae during ripple and IED formation. We observed a potential leading role of cortical neurons in deeper layers, suggesting a refined utilization of LMEs in SOZ localization. LA - English DB - MTMT ER - TY - JOUR AU - Hofer, Katharina AU - Kandrács, Ágnes AU - Tóth, Kinga AU - Hajnal, Boglárka Zsófia AU - Bokodi, Virág AU - Tóth, Estilla Zsófia AU - Erőss, Loránd AU - Entz, László AU - Bagó, Attila György AU - Fabó, Dániel AU - Ulbert, István AU - Wittner, Lucia TI - Bursting of excitatory cells is linked to interictal epileptic discharge generation in humans. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 PG - 17 SN - 2045-2322 DO - 10.1038/s41598-022-10319-4 UR - https://m2.mtmt.hu/api/publication/32785453 ID - 32785453 N1 - Funding Agency and Grant Number: ELKH Research Centre for Natural Sciences; Postdoctoral fellowship of the Hungarian Academy of Sciences; Hungarian Brain Research Program [KTIA_13_NAP-A-IV/1-4,6, KTIA 13 NAP-A-I/1, 2017-1.2.1-NKP-2017-00002, KTIA_NAP_13-1-2013-0001, KTIA-NAP17-3-2017-0001]; Hungarian National Research Fund [OTKA K119443, K137886, PD121123] Funding text: Open access funding provided by ELKH Research Centre for Natural Sciences. This study was supported by the Postdoctoral fellowship of the Hungarian Academy of Sciences (to K. T.), by the Hungarian Brain Research Program, KTIA_13_NAP-A-IV/1-4,6, KTIA 13 NAP-A-I/1 and 2017-1.2.1-NKP-2017-00002 (to I. U.) and KTIA_NAP_13-1-2013-0001, KTIA-NAP17-3-2017-0001 (to D. F.), and by the Hungarian National Research Fund OTKA K119443 and K137886 (to L. W.) and PD121123 (to K. T.) grants. AB - Knowledge about the activity of single neurons is essential in understanding the mechanisms of synchrony generation, and particularly interesting if related to pathological conditions. The generation of interictal spikes-the hypersynchronous events between seizures-is linked to hyperexcitability and to bursting behaviour of neurons in animal models. To explore its cellular mechanisms in humans we investigated the activity of clustered single neurons in a human in vitro model generating both physiological and epileptiform synchronous events. We show that non-epileptic synchronous events resulted from the finely balanced firing of excitatory and inhibitory cells, which was shifted towards an enhanced excitability in epileptic tissue. In contrast, interictal-like spikes were characterised by an asymmetric overall neuronal discharge initiated by excitatory neurons with the presumptive leading role of bursting pyramidal cells, and possibly terminated by inhibitory interneurons. We found that the overall burstiness of human neocortical neurons is not necessarily related to epilepsy, but the bursting behaviour of excitatory cells comprising both intrinsic and synaptically driven bursting is clearly linked to the generation of epileptiform synchrony. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Emília AU - Bokodi, Virág AU - Somogyvári, Zoltán AU - Maglóczky, Zsófia AU - Wittner, Lucia AU - Ulbert, István AU - Erőss, Loránd AU - Fabó, Dániel TI - Laminar distribution of electrically evoked hippocampal short latency ripple activity highlights the importance of the subiculum in vivo in human epilepsy, an intraoperative study JF - EPILEPSY RESEARCH J2 - EPILEPSY RES VL - 169 PY - 2021 PG - 9 SN - 0920-1211 DO - 10.1016/j.eplepsyres.2020.106509 UR - https://m2.mtmt.hu/api/publication/31794996 ID - 31794996 LA - English DB - MTMT ER - TY - JOUR AU - File, Bálint AU - Nánási, Tibor AU - Tóth, E. AU - Bokodi, Virág AU - Tóth, Brigitta AU - Hajnal, Boglárka Zsófia AU - Balogh-Kardos, Zsófia Klára AU - Entz, László AU - Erőss, Loránd AU - Ulbert, István AU - Fabó, Dániel TI - Reorganization of Large-Scale Functional Networks during Low-Frequency Electrical Stimulation of the Cortical Surface JF - INTERNATIONAL JOURNAL OF NEURAL SYSTEMS J2 - INT J NEURAL SYST VL - 30 PY - 2020 IS - 3 PG - 15 SN - 0129-0657 DO - 10.1142/S0129065719500229 UR - https://m2.mtmt.hu/api/publication/30868010 ID - 30868010 N1 - Funding Agency and Grant Number: Hungarian Brain Research Program Grants [2017-1.2.1-NKP-2017-00002]; European Social Fund [EFOP-3.6.3-VEKOP-16-2017-00002] Funding text: BF is supported by the Hungarian Brain Research Program Grants (Grant Nos. 2017-1.2.1-NKP-2017-00002) and the European Social Fund (EFOP-3.6.3-VEKOP-16-2017-00002). The authors are willing to share their data upon personal request. First and second authors contributed equally. LA - English DB - MTMT ER - TY - JOUR AU - Bokodi, Virág TI - Short latency electrically evoked high frequency activity in the human hippocampus in vivo JF - PHD PROCEEDINGS ANNUAL ISSUES OF THE DOCTORAL SCHOOL FACULTY OF INFORMATION TECHNOLOGY AND BIONICS J2 - PHD PROC PPKE IT VL - 11 PY - 2016 SP - 21 EP - 24 PG - 4 SN - 2064-7271 UR - https://m2.mtmt.hu/api/publication/3193263 ID - 3193263 LA - English DB - MTMT ER - TY - CONF AU - Bokodi, Virág AU - Tóth, Emília AU - Somogyvári, Zoltán AU - Maglóczky, Zsófia AU - Erőss, Loránd AU - Ulbert, István AU - Fabó, Dániel TI - High frequency oscillations in the human hippocampal formation using single pulse cortical electrical stimulation [poster] T2 - IBRO Workshop 2016 PY - 2016 PG - 1 UR - https://m2.mtmt.hu/api/publication/3015752 ID - 3015752 LA - English DB - MTMT ER - TY - JOUR AU - Bokodi, Virág TI - Evoked high frequency oscillations in human hippocampus JF - PHD PROCEEDINGS ANNUAL ISSUES OF THE DOCTORAL SCHOOL FACULTY OF INFORMATION TECHNOLOGY AND BIONICS J2 - PHD PROC PPKE IT VL - 10 PY - 2015 SP - 17 EP - 20 PG - 4 SN - 2064-7271 UR - https://m2.mtmt.hu/api/publication/3192399 ID - 3192399 LA - English DB - MTMT ER - TY - GEN AU - Bokodi, Virág AU - Emília, Tóth AU - Zsófia, Maglóczky AU - Entz, László AU - Erőss, Loránd AU - István, Ulbert AU - Fabó, Dániel TI - Evoked high frequency oscillations in the human hippocampal formation [lecture] PY - 2015 UR - https://m2.mtmt.hu/api/publication/3028097 ID - 3028097 LA - English DB - MTMT ER -