TY - JOUR AU - Jenei, Kinga AU - Szatmári, Ildikó AU - Szabó, Eszter AU - Mariam, Anjum AU - Luczay, Andrea AU - Zsidegh, Petra AU - Tóth-Heyn, Péter TI - Laktátszintváltozások diabeteses ketoacidosisban és frissen diagnosztizált 1-es típusú diabetes mellitusban [Changes of lactate levels in diabetic ketoacidosis and in newly diagnosed type 1 diabetes mellitus] JF - ORVOSI HETILAP J2 - ORV HETIL VL - 160 PY - 2019 IS - 45 SP - 1784 EP - 1790 PG - 7 SN - 0030-6002 DO - 10.1556/650.2019.31533 UR - https://m2.mtmt.hu/api/publication/30914804 ID - 30914804 LA - Hungarian DB - MTMT ER - TY - THES AU - Szabó, Eszter TI - Új tömegspektrometriás megközelítések a veleszületett anyagcsere-betegségek szűrésében és diagnosztikájában PB - Eötvös Loránd Tudományegyetem (ELTE) PY - 2019 SP - 104 DO - 10.15476/ELTE.2018.136 UR - https://m2.mtmt.hu/api/publication/30649157 ID - 30649157 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szabó, Eszter AU - Balogh, Lídia AU - Szabó, Attila AU - Szatmári, Ildikó TI - Ritka örökletes anyagcsere-betegségek diagnosztikája. laboratóriumi vizsgálati megközelítések TS - laboratóriumi vizsgálati megközelítések JF - ORVOSI HETILAP J2 - ORV HETIL VL - 158 PY - 2017 IS - 48 SP - 1903 EP - 1907 PG - 5 SN - 0030-6002 DO - 10.1556/650.2017.30899 UR - https://m2.mtmt.hu/api/publication/3296060 ID - 3296060 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Molvarec, Attila AU - Fügedi, Gergely AU - Molnár, Miklós AU - Szabó, Eszter AU - Schönléber, Julianna AU - Rigó, János TI - Evaluation of the endocannabinoid system in preeclampsia JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 5 PY - 2015 IS - 3 SP - 212 SN - 2210-7789 DO - 10.1016/j.preghy.2015.07.011 UR - https://m2.mtmt.hu/api/publication/3009599 ID - 3009599 AB - Introduction The endocannabinoid system plays a key role in female reproduction, including implantation, decidualization and placentation. A growing number of studies indicate that placental and peripheral blood anandamide levels correlate closely with both spontaneous miscarriage and ectopic pregnancy. Anandamide has also been implicated in blood pressure regulation. Objectives In this study, we aimed to analyze placental expression and localization of cannabinoid receptor 1 (CB1), CB2 and fatty acid amid hydrolase (FAAH), as well as circulating anandamide levels in normal pregnancy and preeclampsia. Methods We determined CB1, CB2 and FAAH expressions by Western blotting and immunohistochemistry in placental samples collected directly after Cesarean section in 18 preeclamptic patients and 18 normotensive, healthy pregnant women. Serum anandamide concentrations were measured by high performance liquid chromatography–mass spectrometry (HPLC–MS) technique in 43 preeclamptic patients and 71 healthy pregnant women. Serum total soluble fms-like tyrosine kinase-1 (sFlt-1) and biologically active placental growth factor (PlGF) levels were assessed by electrochemiluminescence immunoassay. Results CB1 expression semi-quantified by Western blotting was significantly higher in preeclamptic placenta, and these findings were confirmed by immunohistochemistry. CB1 immunoreactivity was markedly stronger in syncytiotrophoblasts, the mesenchymal core, decidua, villous capillary endothelial and smooth muscle cells, as well as in the amnion in preeclamptic samples compared to normal pregnancies. However, we did not find significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity. Serum levels of anandamide were significantly lower in preeclamptic patients than in healthy pregnant women. Preeclamptic patients had significantly higher sFlt-1 levels and significantly lower PlGF concentrations as compared to healthy pregnant women. Serum anandamide concentrations did not correlate with serum levels of sFlt-1 and PlGF in our healthy pregnant and preeclamptic groups. Conclusion We observed markedly higher expression of CB1 protein in preeclamptic placental tissue. Increased CB1 expression might cause abnormal decidualization and impair trophoblast invasion, thus being involved in the pathogenesis of preeclampsia. Nevertheless, we did not find significant differences between preeclamptic and normal placental tissue regarding CB2 and FAAH expressions. Furthermore, serum anandamide concentrations were decreased in women with preeclampsia. While the detailed pathogenesis of preeclampsia is still unclear, the endocannabinoid system could play a role in the development of the disease. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Eszter AU - Szatmári, Ildikó AU - Szőnyi, László AU - Takats, Z TI - Quantitative Analytical Method for the Determination of Biotinidase Activity in Dried Blood Spot Samples. JF - ANALYTICAL CHEMISTRY J2 - ANAL CHEM VL - 87 PY - 2015 IS - 20 SP - 10573 EP - 10578 PG - 6 SN - 0003-2700 DO - 10.1021/acs.analchem.5b02996 UR - https://m2.mtmt.hu/api/publication/2976357 ID - 2976357 AB - Biotinidase activity assay is included in most newborn screening protocols, and the positive results are confirmed by quantitative enzyme activity measurements. In our study, we describe a new quantitative analytical method for the determination of biotinidase activity using the blood sample deposited onto filter paper as the assay medium, by predepositing N-biotinyl-p-aminobenzoic acid onto the standard sample collection paper. The analysis of the assay mixture requires a simple extraction step from a dried blood spot followed by the quantification of product by LC-MS. The method provides a simple and reliable enzyme assay method that enables the rapid diagnosis of biotinidase deficiency (BD). Out of the measured 36 samples, 13 were healthy with lower enzyme activities, 16 were patients with partial BD, and 7 were patients with profound BD with residual activity below 10%. Expression of enzyme activity in percentage of mean activity of negative controls allows comparison of the different techniques. The obtained results are in good agreement with activity data determined from both dried blood spots and serum samples, giving an informative diagnostic value. LA - English DB - MTMT ER - TY - JOUR AU - Turmezeiné Horváth, Judit AU - Jávorszky, Eszter AU - Szabó, Eszter AU - Dredán, Judit AU - Kállai-Szabó, Barnabás AU - Zelkó, Romána TI - EFFECT OF STORAGE TEMPERATURE ON THE STABILITY OF TOTAL PARENTERAL NUTRITION ADMIXTURES PREPARED FOR INFANTS JF - ACTA POLONIAE PHARMACEUTICA: DRUG RESEARCH J2 - ACTA POL PHARM VL - 72 PY - 2015 IS - 5 SP - 843 EP - 849 PG - 7 SN - 0001-6837 UR - https://m2.mtmt.hu/api/publication/2941106 ID - 2941106 LA - English DB - MTMT ER - TY - JOUR AU - Molvarec, Attila AU - Fügedi, Gergely AU - Szabó, Eszter AU - Stenczer, Balázs AU - Walentin, S AU - Rigó, János TI - Decreased circulating anandamide levels in preeclampsia. JF - HYPERTENSION RESEARCH J2 - HYPERTENS RES VL - 38 PY - 2015 IS - 6 SP - 413 EP - 418 PG - 6 SN - 0916-9636 DO - 10.1038/hr.2015.20 UR - https://m2.mtmt.hu/api/publication/2851443 ID - 2851443 AB - The endocannabinoid system has a key role in female reproduction, including implantation, decidualization and placentation. A growing number of studies indicate that placental and peripheral blood anandamide levels correlate closely with both spontaneous miscarriage and ectopic pregnancy. Anandamide has also been implicated in blood pressure regulation. In this study, we aimed to determine circulating anandamide levels in preeclampsia for the first time in the literature. Forty-three preeclamptic patients and 71 healthy pregnant women were involved in this case-control study. Serum anandamide concentrations were determined by high-performance liquid chromatography-mass spectrometry technique. Serum total soluble fms-like tyrosine kinase-1 (sFlt-1) and biologically active placental growth factor (PlGF) levels were measured by electrochemiluminescence immunoassay. For statistical analyses, nonparametric methods were applied. Serum levels of anandamide were significantly lower in preeclamptic patients than in healthy pregnant women (0.75 (0.44-1.03) ng ml-1 vs. 1.30 (0.76-2.0) ng ml-1, P<0.001). Preeclamptic patients had significantly higher sFlt-1 levels (12 121 (7963-18 316) pg ml-1 vs. 2299 (1393-3179) pg ml-1, P<0.001) and significantly lower PlGF concentrations (71.2 (39.2-86.4) pg ml-1 vs. 256.8 (181.1-421.0) pg ml-1, P<0.001) as compared with healthy pregnant women. Serum anandamide concentrations did not correlate with serum levels of sFlt-1 and PlGF in our healthy pregnant and preeclamptic groups. In conclusion, we demonstrated for the first time in the literature that serum anandamide concentrations are decreased in women with preeclampsia. However, the cause and consequence of this observation remain to be determined.Hypertension Research advance online publication, 26 February 2015; doi:10.1038/hr.2015.20. LA - English DB - MTMT ER - TY - JOUR AU - Szekeres, Mária AU - Nádasy, György László AU - Turu, Gábor AU - Soltész-Katona, Eszter AU - Benyó, Zoltán AU - Offermanns, S AU - Ruisanchez, Éva AU - Szabó, Eszter AU - Takats, Z AU - Batkai, S AU - Tóth, Zsuzsanna AU - Hunyady, László TI - Endocannabinoid-mediated modulation of Gq/11 protein-coupled receptor signaling-induced vasoconstriction and hypertension JF - MOLECULAR AND CELLULAR ENDOCRINOLOGY J2 - MOL CELL ENDOCRINOL VL - 403 PY - 2015 SP - 46 EP - 56 PG - 11 SN - 0303-7207 DO - 10.1016/j.mce.2015.01.012 UR - https://m2.mtmt.hu/api/publication/2833239 ID - 2833239 N1 - Department of Physiology, Semmelweis University, Budapest, Hungary Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary Department of Pharmacology, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary Institute of Molecular and Translational Therapeutic Strategies, Hannover Medical School, Hannover, Germany Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary MTA-SE Laboratory of Molecular Physiology, Budapest, Hungary Cited By :15 Export Date: 31 August 2019 CODEN: MCEND Correspondence Address: Hunyady, L.; Department of Physiology, Semmelweis University, P.O.Box 259, Hungary; email: hunyady@eok.sote.hu Chemicals/CAS: 4 [bis(1,3 benzodioxol 5 yl)hydroxymethyl] 1 piperidinecarboxylic acid 4 nitrophenyl ester, 1101854-58-3; acylglycerol, 64706-27-0; acylglycerol lipase, 9040-75-9; angiotensin II, 11128-99-7; calcium, 7440-70-2, 14092-94-5; calcium ion, 14127-61-8; lactone, 1338-03-0; lipoprotein lipase, 83137-80-8, 9004-02-8; phenylephrine, 532-38-7, 59-42-7, 61-76-7; prostaglandin F2 alpha, 551-11-1; tetrahydrolipstatin, 96829-58-2; 2-arachidonylglycerol; Angiotensin II; Arachidonic Acids; Benzodioxoles; Calcium; Dinoprost; Endocannabinoids; Glycerides; GTP-Binding Protein alpha Subunits, Gq-G11; JZL 184; Lactones; Lipoprotein Lipase; Monoacylglycerol Lipases; orlistat; Phenylephrine; Piperidines; Receptor, Cannabinoid, CB1 Funding details: NK-100883, K-101775 Funding details: National Science and Technology Development Agency, TÁMOP 4.2.1.B-09/1/KMR-2010-0001 Funding details: European Commission, FP7-PEOPLE-2011-CIG 294278 Funding text 1: The authors are grateful to Dr. Andras Balla, Dr. Csaba Ádori, Dr. Péter Várnai (Semmelweis University, Budapest), Dr. Andreas Zimmer (University of Bonn) and Dr. István Katona (Institute of Exp. Med. of the Hungarian Academy of Sciences, Budapest) for helpful discussions on the manuscript and to Judit Rácz, Mártonné Schulcz, Ilona Oláh, László Hricisák, Gergely Tóth and Ildikó Oravecz for their expert assistances. Sources of Funding: This work was supported by grants from the Hungarian National Science Foundation OTKA NK-100883 , K-101775 , from the National Development Agency, Hungary ( TÁMOP 4.2.1.B-09/1/KMR-2010-0001 ) and from the European Union ( FP7-PEOPLE-2011-CIG 294278 ). Appendix Department of Physiology, Semmelweis University, Budapest, Hungary Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary Department of Pharmacology, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary Institute of Molecular and Translational Therapeutic Strategies, Hannover Medical School, Hannover, Germany Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary MTA-SE Laboratory of Molecular Physiology, Budapest, Hungary Cited By :15 Export Date: 2 September 2019 CODEN: MCEND Correspondence Address: Hunyady, L.; Department of Physiology, Semmelweis University, P.O.Box 259, Hungary; email: hunyady@eok.sote.hu Chemicals/CAS: 4 [bis(1,3 benzodioxol 5 yl)hydroxymethyl] 1 piperidinecarboxylic acid 4 nitrophenyl ester, 1101854-58-3; acylglycerol, 64706-27-0; acylglycerol lipase, 9040-75-9; angiotensin II, 11128-99-7; calcium, 7440-70-2, 14092-94-5; calcium ion, 14127-61-8; lactone, 1338-03-0; lipoprotein lipase, 83137-80-8, 9004-02-8; phenylephrine, 532-38-7, 59-42-7, 61-76-7; prostaglandin F2 alpha, 551-11-1; tetrahydrolipstatin, 96829-58-2; 2-arachidonylglycerol; Angiotensin II; Arachidonic Acids; Benzodioxoles; Calcium; Dinoprost; Endocannabinoids; Glycerides; GTP-Binding Protein alpha Subunits, Gq-G11; JZL 184; Lactones; Lipoprotein Lipase; Monoacylglycerol Lipases; orlistat; Phenylephrine; Piperidines; Receptor, Cannabinoid, CB1 Funding details: NK-100883, K-101775 Funding details: National Science and Technology Development Agency, TÁMOP 4.2.1.B-09/1/KMR-2010-0001 Funding details: European Commission, FP7-PEOPLE-2011-CIG 294278 Funding text 1: The authors are grateful to Dr. Andras Balla, Dr. Csaba Ádori, Dr. Péter Várnai (Semmelweis University, Budapest), Dr. Andreas Zimmer (University of Bonn) and Dr. István Katona (Institute of Exp. Med. of the Hungarian Academy of Sciences, Budapest) for helpful discussions on the manuscript and to Judit Rácz, Mártonné Schulcz, Ilona Oláh, László Hricisák, Gergely Tóth and Ildikó Oravecz for their expert assistances. Sources of Funding: This work was supported by grants from the Hungarian National Science Foundation OTKA NK-100883 , K-101775 , from the National Development Agency, Hungary ( TÁMOP 4.2.1.B-09/1/KMR-2010-0001 ) and from the European Union ( FP7-PEOPLE-2011-CIG 294278 ). Appendix AB - Activation of G protein-coupled receptors (GPCRs) can induce vasoconstriction via calcium signal-mediated and Rho-dependent pathways. Earlier reports have shown that diacylglycerol produced during calcium signal generation can be converted to an endocannabinoid, 2-arachidonoylglycerol (2-AG). Our aim was to provide evidence that GPCR signaling-induced 2-AG production and activation of vascular type1 cannabinoid receptors (CB1R) is capable of reducing agonist-induced vasoconstriction and hypertension. Rat and mouse aortic rings were examined by myography. Vascular expression of CB1R was demonstrated with immunohistochemistry. Rat aortic vascular smooth muscle cells (VSMCs) were cultured for calcium measurements and 2-AG-determination. Inhibition or genetic loss of CB1Rs enhanced vasoconstriction induced by angiotensin II (AngII) or phenylephrine (Phe), but not by prostaglandin(PG)F2alpha. AngII-induced vasoconstriction was augmented by inhibition of diacylglycerol lipase (tetrahydrolipstatin) and was attenuated by inhibition of monoacylglycerol lipase (JZL184) suggesting a functionally relevant role for endogenously produced 2-AG. In Galphaq/11-deficient mice vasoconstriction was absent to AngII or Phe, which activate Gq/11-coupled receptors, but was maintained in response to PGF2alpha. In VSMCs, AngII-stimulated 2-AG-formation was inhibited by tetrahydrolipstatin and potentiated by JZL184. CB1R inhibition increased the sustained phase of AngII-induced calcium signal. Pharmacological or genetic loss of CB1R function augmented AngII-induced blood pressure rise in mice. These data demonstrate that vasoconstrictor effect of GPCR agonists is attenuated via Gq/11-mediated vascular endocannabinoid formation. Agonist-induced endocannabinoid-mediated CB1R activation is a significant physiological modulator of vascular tone. Thus, the selective modulation of GPCR signaling-induced endocannabinoid release has a therapeutic potential in case of increased vascular tone and hypertension. LA - English DB - MTMT ER - TY - JOUR AU - Szőnyi, László AU - Szatmári, Ildikó AU - Szabó, Eszter TI - Szérumbiotinidáz-aktivitás vizsgálata glikogenózisban JF - GYERMEKGYÓGYÁSZAT J2 - GYERMEKGYÓGYÁSZAT VL - 65 PY - 2014 IS - 3 SP - 197 SN - 0017-5900 UR - https://m2.mtmt.hu/api/publication/2602494 ID - 2602494 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Denes, J AU - Szabó, Eszter AU - Robinette, SL AU - Szatmári, Ildikó AU - Szőnyi, László AU - Kreuder, JG AU - Rauterberg, EW AU - Takats, Z TI - Metabonomics of newborn screening dried blood spot samples: a novel approach in the screening and diagnostics of inborn errors of metabolism. JF - ANALYTICAL CHEMISTRY J2 - ANAL CHEM VL - 84 PY - 2012 IS - 22 SP - 10113 EP - 10120 PG - 8 SN - 0003-2700 DO - 10.1021/ac302527m UR - https://m2.mtmt.hu/api/publication/2425472 ID - 2425472 AB - A novel, single stage high resolution mass spectrometry-based method is presented for the population level screening of inborn errors of metabolism. The approach proposed here extends traditional electrospray tandem mass spectrometry screening by introducing nanospray ionization and high resolution mass spectrometry, allowing the selective detection of more than 400 individual metabolic constituents of blood including acylcarnitines, amino acids, organic acids, fatty acids, carbohydrates, bile acids, and complex lipids. Dried blood spots were extracted using a methanolic solution of isotope labeled internal standards, and filtered extracts were electrosprayed using a fully automated chip-based nanospray ion source in both positive and negative ion mode. Ions were analyzed using an Orbitrap Fourier transformation mass spectrometer at nominal mass resolution of 100,000. Individual metabolic constituents were quantified using standard isotope dilution methods. Concentration threshold (cutoff) level-based analysis allows the identification of newborns with metabolic diseases, similarly to the traditional electrospray tandem mass spectrometry (ESI-MS/MS) method; however, the detection of additional known biomarkers (e.g., organic acids) results in improved sensitivity and selectivity. The broad range of detected analytes allowed the untargeted multivariate statistical analysis of spectra and identification of additional diseased states, therapeutic artifacts, and damaged samples, besides the metabolic disease panel. LA - English DB - MTMT ER -