@article{MTMT:30914804, title = {Laktátszintváltozások diabeteses ketoacidosisban és frissen diagnosztizált 1-es típusú diabetes mellitusban [Changes of lactate levels in diabetic ketoacidosis and in newly diagnosed type 1 diabetes mellitus]}, url = {https://m2.mtmt.hu/api/publication/30914804}, author = {Jenei, Kinga and Szatmári, Ildikó and Szabó, Eszter and Mariam, Anjum and Luczay, Andrea and Zsidegh, Petra and Tóth-Heyn, Péter}, doi = {10.1556/650.2019.31533}, journal-iso = {ORV HETIL}, journal = {ORVOSI HETILAP}, volume = {160}, unique-id = {30914804}, issn = {0030-6002}, year = {2019}, eissn = {1788-6120}, pages = {1784-1790}, orcid-numbers = {Szatmári, Ildikó/0000-0003-1040-145X; Szabó, Eszter/0000-0003-4054-5032; Luczay, Andrea/0000-0002-3877-1471; Zsidegh, Petra/0000-0003-2931-2633; Tóth-Heyn, Péter/0000-0002-7521-5044} } @mastersthesis{MTMT:30649157, title = {Új tömegspektrometriás megközelítések a veleszületett anyagcsere-betegségek szűrésében és diagnosztikájában}, url = {https://m2.mtmt.hu/api/publication/30649157}, author = {Szabó, Eszter}, doi = {10.15476/ELTE.2018.136}, publisher = {Eötvös Loránd University}, unique-id = {30649157}, year = {2019}, orcid-numbers = {Szabó, Eszter/0000-0003-4054-5032} } @article{MTMT:3296060, title = {Ritka örökletes anyagcsere-betegségek diagnosztikája. laboratóriumi vizsgálati megközelítések}, url = {https://m2.mtmt.hu/api/publication/3296060}, author = {Szabó, Eszter and Balogh, Lídia and Szabó, Attila and Szatmári, Ildikó}, doi = {10.1556/650.2017.30899}, journal-iso = {ORV HETIL}, journal = {ORVOSI HETILAP}, volume = {158}, unique-id = {3296060}, issn = {0030-6002}, year = {2017}, eissn = {1788-6120}, pages = {1903-1907}, orcid-numbers = {Szabó, Eszter/0000-0003-4054-5032; Balogh, Lídia/0000-0003-1018-3560; Szabó, Attila/0000-0001-7321-9861; Szatmári, Ildikó/0000-0003-1040-145X} } @article{MTMT:3009599, title = {Evaluation of the endocannabinoid system in preeclampsia}, url = {https://m2.mtmt.hu/api/publication/3009599}, author = {Molvarec, Attila and Fügedi, Gergely and Molnár, Miklós and Szabó, Eszter and Schönléber, Julianna and Rigó, János}, doi = {10.1016/j.preghy.2015.07.011}, journal-iso = {PREGNANCY HYPERTENS}, journal = {PREGNANCY HYPERTENSION}, volume = {5}, unique-id = {3009599}, issn = {2210-7789}, abstract = {Introduction The endocannabinoid system plays a key role in female reproduction, including implantation, decidualization and placentation. A growing number of studies indicate that placental and peripheral blood anandamide levels correlate closely with both spontaneous miscarriage and ectopic pregnancy. Anandamide has also been implicated in blood pressure regulation. Objectives In this study, we aimed to analyze placental expression and localization of cannabinoid receptor 1 (CB1), CB2 and fatty acid amid hydrolase (FAAH), as well as circulating anandamide levels in normal pregnancy and preeclampsia. Methods We determined CB1, CB2 and FAAH expressions by Western blotting and immunohistochemistry in placental samples collected directly after Cesarean section in 18 preeclamptic patients and 18 normotensive, healthy pregnant women. Serum anandamide concentrations were measured by high performance liquid chromatography–mass spectrometry (HPLC–MS) technique in 43 preeclamptic patients and 71 healthy pregnant women. Serum total soluble fms-like tyrosine kinase-1 (sFlt-1) and biologically active placental growth factor (PlGF) levels were assessed by electrochemiluminescence immunoassay. Results CB1 expression semi-quantified by Western blotting was significantly higher in preeclamptic placenta, and these findings were confirmed by immunohistochemistry. CB1 immunoreactivity was markedly stronger in syncytiotrophoblasts, the mesenchymal core, decidua, villous capillary endothelial and smooth muscle cells, as well as in the amnion in preeclamptic samples compared to normal pregnancies. However, we did not find significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity. Serum levels of anandamide were significantly lower in preeclamptic patients than in healthy pregnant women. Preeclamptic patients had significantly higher sFlt-1 levels and significantly lower PlGF concentrations as compared to healthy pregnant women. Serum anandamide concentrations did not correlate with serum levels of sFlt-1 and PlGF in our healthy pregnant and preeclamptic groups. Conclusion We observed markedly higher expression of CB1 protein in preeclamptic placental tissue. Increased CB1 expression might cause abnormal decidualization and impair trophoblast invasion, thus being involved in the pathogenesis of preeclampsia. Nevertheless, we did not find significant differences between preeclamptic and normal placental tissue regarding CB2 and FAAH expressions. Furthermore, serum anandamide concentrations were decreased in women with preeclampsia. While the detailed pathogenesis of preeclampsia is still unclear, the endocannabinoid system could play a role in the development of the disease.}, year = {2015}, eissn = {2210-7797}, pages = {212}, orcid-numbers = {Molvarec, Attila/0000-0002-3229-3034; Fügedi, Gergely/0000-0003-3370-6766; Molnár, Miklós/0000-0001-9231-782X; Szabó, Eszter/0000-0003-4054-5032; Rigó, János/0000-0003-2762-6516} } @article{MTMT:2976357, title = {Quantitative Analytical Method for the Determination of Biotinidase Activity in Dried Blood Spot Samples.}, url = {https://m2.mtmt.hu/api/publication/2976357}, author = {Szabó, Eszter and Szatmári, Ildikó and Szőnyi, László and Takats, Z}, doi = {10.1021/acs.analchem.5b02996}, journal-iso = {ANAL CHEM}, journal = {ANALYTICAL CHEMISTRY}, volume = {87}, unique-id = {2976357}, issn = {0003-2700}, abstract = {Biotinidase activity assay is included in most newborn screening protocols, and the positive results are confirmed by quantitative enzyme activity measurements. In our study, we describe a new quantitative analytical method for the determination of biotinidase activity using the blood sample deposited onto filter paper as the assay medium, by predepositing N-biotinyl-p-aminobenzoic acid onto the standard sample collection paper. The analysis of the assay mixture requires a simple extraction step from a dried blood spot followed by the quantification of product by LC-MS. The method provides a simple and reliable enzyme assay method that enables the rapid diagnosis of biotinidase deficiency (BD). Out of the measured 36 samples, 13 were healthy with lower enzyme activities, 16 were patients with partial BD, and 7 were patients with profound BD with residual activity below 10%. Expression of enzyme activity in percentage of mean activity of negative controls allows comparison of the different techniques. The obtained results are in good agreement with activity data determined from both dried blood spots and serum samples, giving an informative diagnostic value.}, year = {2015}, eissn = {1520-6882}, pages = {10573-10578}, orcid-numbers = {Szabó, Eszter/0000-0003-4054-5032; Szatmári, Ildikó/0000-0003-1040-145X; Szőnyi, László/0000-0002-1140-1913} } @article{MTMT:2941106, title = {EFFECT OF STORAGE TEMPERATURE ON THE STABILITY OF TOTAL PARENTERAL NUTRITION ADMIXTURES PREPARED FOR INFANTS}, url = {https://m2.mtmt.hu/api/publication/2941106}, author = {Turmezeiné Horváth, Judit and Jávorszky, Eszter and Szabó, Eszter and Dredán, Judit and Kállai-Szabó, Barnabás and Zelkó, Romána}, journal-iso = {ACTA POL PHARM}, journal = {ACTA POLONIAE PHARMACEUTICA: DRUG RESEARCH}, volume = {72}, unique-id = {2941106}, issn = {0001-6837}, year = {2015}, eissn = {2353-5288}, pages = {843-849}, orcid-numbers = {Turmezeiné Horváth, Judit/0000-0002-1745-1081; Jávorszky, Eszter/0000-0002-7834-8854; Szabó, Eszter/0000-0003-4054-5032; Dredán, Judit/0000-0001-5278-8053; Kállai-Szabó, Barnabás/0000-0001-6259-4818; Zelkó, Romána/0000-0002-5419-9137} } @article{MTMT:2851443, title = {Decreased circulating anandamide levels in preeclampsia.}, url = {https://m2.mtmt.hu/api/publication/2851443}, author = {Molvarec, Attila and Fügedi, Gergely and Szabó, Eszter and Stenczer, Balázs and Walentin, S and Rigó, János}, doi = {10.1038/hr.2015.20}, journal-iso = {HYPERTENS RES}, journal = {HYPERTENSION RESEARCH}, volume = {38}, unique-id = {2851443}, issn = {0916-9636}, abstract = {The endocannabinoid system has a key role in female reproduction, including implantation, decidualization and placentation. A growing number of studies indicate that placental and peripheral blood anandamide levels correlate closely with both spontaneous miscarriage and ectopic pregnancy. Anandamide has also been implicated in blood pressure regulation. In this study, we aimed to determine circulating anandamide levels in preeclampsia for the first time in the literature. Forty-three preeclamptic patients and 71 healthy pregnant women were involved in this case-control study. Serum anandamide concentrations were determined by high-performance liquid chromatography-mass spectrometry technique. Serum total soluble fms-like tyrosine kinase-1 (sFlt-1) and biologically active placental growth factor (PlGF) levels were measured by electrochemiluminescence immunoassay. For statistical analyses, nonparametric methods were applied. Serum levels of anandamide were significantly lower in preeclamptic patients than in healthy pregnant women (0.75 (0.44-1.03) ng ml-1 vs. 1.30 (0.76-2.0) ng ml-1, P<0.001). Preeclamptic patients had significantly higher sFlt-1 levels (12 121 (7963-18 316) pg ml-1 vs. 2299 (1393-3179) pg ml-1, P<0.001) and significantly lower PlGF concentrations (71.2 (39.2-86.4) pg ml-1 vs. 256.8 (181.1-421.0) pg ml-1, P<0.001) as compared with healthy pregnant women. Serum anandamide concentrations did not correlate with serum levels of sFlt-1 and PlGF in our healthy pregnant and preeclamptic groups. In conclusion, we demonstrated for the first time in the literature that serum anandamide concentrations are decreased in women with preeclampsia. However, the cause and consequence of this observation remain to be determined.Hypertension Research advance online publication, 26 February 2015; doi:10.1038/hr.2015.20.}, year = {2015}, eissn = {1348-4214}, pages = {413-418}, orcid-numbers = {Molvarec, Attila/0000-0002-3229-3034; Fügedi, Gergely/0000-0003-3370-6766; Szabó, Eszter/0000-0003-4054-5032; Rigó, János/0000-0003-2762-6516} } @article{MTMT:2833239, title = {Endocannabinoid-mediated modulation of Gq/11 protein-coupled receptor signaling-induced vasoconstriction and hypertension}, url = {https://m2.mtmt.hu/api/publication/2833239}, author = {Szekeres, Mária and Nádasy, György László and Turu, Gábor and Soltész-Katona, Eszter and Benyó, Zoltán and Offermanns, S and Ruisanchez, Éva and Szabó, Eszter and Takats, Z and Batkai, S and Tóth, Zsuzsanna and Hunyady, László}, doi = {10.1016/j.mce.2015.01.012}, journal-iso = {MOL CELL ENDOCRINOL}, journal = {MOLECULAR AND CELLULAR ENDOCRINOLOGY}, volume = {403}, unique-id = {2833239}, issn = {0303-7207}, abstract = {Activation of G protein-coupled receptors (GPCRs) can induce vasoconstriction via calcium signal-mediated and Rho-dependent pathways. Earlier reports have shown that diacylglycerol produced during calcium signal generation can be converted to an endocannabinoid, 2-arachidonoylglycerol (2-AG). Our aim was to provide evidence that GPCR signaling-induced 2-AG production and activation of vascular type1 cannabinoid receptors (CB1R) is capable of reducing agonist-induced vasoconstriction and hypertension. Rat and mouse aortic rings were examined by myography. Vascular expression of CB1R was demonstrated with immunohistochemistry. Rat aortic vascular smooth muscle cells (VSMCs) were cultured for calcium measurements and 2-AG-determination. Inhibition or genetic loss of CB1Rs enhanced vasoconstriction induced by angiotensin II (AngII) or phenylephrine (Phe), but not by prostaglandin(PG)F2alpha. AngII-induced vasoconstriction was augmented by inhibition of diacylglycerol lipase (tetrahydrolipstatin) and was attenuated by inhibition of monoacylglycerol lipase (JZL184) suggesting a functionally relevant role for endogenously produced 2-AG. In Galphaq/11-deficient mice vasoconstriction was absent to AngII or Phe, which activate Gq/11-coupled receptors, but was maintained in response to PGF2alpha. In VSMCs, AngII-stimulated 2-AG-formation was inhibited by tetrahydrolipstatin and potentiated by JZL184. CB1R inhibition increased the sustained phase of AngII-induced calcium signal. Pharmacological or genetic loss of CB1R function augmented AngII-induced blood pressure rise in mice. These data demonstrate that vasoconstrictor effect of GPCR agonists is attenuated via Gq/11-mediated vascular endocannabinoid formation. Agonist-induced endocannabinoid-mediated CB1R activation is a significant physiological modulator of vascular tone. Thus, the selective modulation of GPCR signaling-induced endocannabinoid release has a therapeutic potential in case of increased vascular tone and hypertension.}, year = {2015}, eissn = {1872-8057}, pages = {46-56}, orcid-numbers = {Szekeres, Mária/0000-0002-6358-7270; Nádasy, György László/0000-0003-2057-2391; Turu, Gábor/0000-0002-4421-3812; Soltész-Katona, Eszter/0000-0002-9054-740X; Benyó, Zoltán/0000-0001-6015-0359; Ruisanchez, Éva/0000-0001-7779-226X; Szabó, Eszter/0000-0003-4054-5032; Tóth, Zsuzsanna/0000-0002-0628-1320; Hunyady, László/0000-0002-8438-7251} } @article{MTMT:2602494, title = {Szérumbiotinidáz-aktivitás vizsgálata glikogenózisban}, url = {https://m2.mtmt.hu/api/publication/2602494}, author = {Szőnyi, László and Szatmári, Ildikó and Szabó, Eszter}, journal-iso = {GYERMEKGYÓGYÁSZAT}, journal = {GYERMEKGYÓGYÁSZAT}, volume = {65}, unique-id = {2602494}, issn = {0017-5900}, year = {2014}, pages = {197}, orcid-numbers = {Szőnyi, László/0000-0002-1140-1913; Szatmári, Ildikó/0000-0003-1040-145X; Szabó, Eszter/0000-0003-4054-5032} } @article{MTMT:2425472, title = {Metabonomics of newborn screening dried blood spot samples: a novel approach in the screening and diagnostics of inborn errors of metabolism.}, url = {https://m2.mtmt.hu/api/publication/2425472}, author = {Denes, J and Szabó, Eszter and Robinette, SL and Szatmári, Ildikó and Szőnyi, László and Kreuder, JG and Rauterberg, EW and Takats, Z}, doi = {10.1021/ac302527m}, journal-iso = {ANAL CHEM}, journal = {ANALYTICAL CHEMISTRY}, volume = {84}, unique-id = {2425472}, issn = {0003-2700}, abstract = {A novel, single stage high resolution mass spectrometry-based method is presented for the population level screening of inborn errors of metabolism. The approach proposed here extends traditional electrospray tandem mass spectrometry screening by introducing nanospray ionization and high resolution mass spectrometry, allowing the selective detection of more than 400 individual metabolic constituents of blood including acylcarnitines, amino acids, organic acids, fatty acids, carbohydrates, bile acids, and complex lipids. Dried blood spots were extracted using a methanolic solution of isotope labeled internal standards, and filtered extracts were electrosprayed using a fully automated chip-based nanospray ion source in both positive and negative ion mode. Ions were analyzed using an Orbitrap Fourier transformation mass spectrometer at nominal mass resolution of 100,000. Individual metabolic constituents were quantified using standard isotope dilution methods. Concentration threshold (cutoff) level-based analysis allows the identification of newborns with metabolic diseases, similarly to the traditional electrospray tandem mass spectrometry (ESI-MS/MS) method; however, the detection of additional known biomarkers (e.g., organic acids) results in improved sensitivity and selectivity. The broad range of detected analytes allowed the untargeted multivariate statistical analysis of spectra and identification of additional diseased states, therapeutic artifacts, and damaged samples, besides the metabolic disease panel.}, year = {2012}, eissn = {1520-6882}, pages = {10113-10120}, orcid-numbers = {Szabó, Eszter/0000-0003-4054-5032; Szatmári, Ildikó/0000-0003-1040-145X; Szőnyi, László/0000-0002-1140-1913} }