TY - JOUR AU - Szabó, Enikő AU - Hornung, Ákos AU - Monostori, Éva AU - Bocskai, Márta AU - Czibula, Ágnes AU - Kovács, László TI - Altered Cell Surface N-Glycosylation of Resting and Activated T Cells in Systemic Lupus Erythematosus JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 20 PY - 2019 IS - 18 PG - 14 SN - 1661-6596 DO - 10.3390/ijms20184455 UR - https://m2.mtmt.hu/api/publication/30802849 ID - 30802849 N1 - Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Rheumatology and Immunology, Faculty of Medicine, University of Szeged, Szeged, 6725, Hungary Export Date: 29 November 2019 Correspondence Address: Czibula, Á.; Institute of Genetics, Biological Research Centre of the Hungarian Academy of SciencesHungary; email: czibula.agnes@brc.hu Chemicals/CAS: galectin 1, 258495-34-0; sialidase, 9001-67-6; sialyltransferase, 9075-81-4 Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Rheumatology and Immunology, Faculty of Medicine, University of Szeged, Szeged, 6725, Hungary Export Date: 2 December 2019 Correspondence Address: Czibula, Á.; Institute of Genetics, Biological Research Centre of the Hungarian Academy of SciencesHungary; email: czibula.agnes@brc.hu Chemicals/CAS: galectin 1, 258495-34-0; sialidase, 9001-67-6; sialyltransferase, 9075-81-4 LA - English DB - MTMT ER - TY - THES AU - Hornung, Ákos TI - Human immunosuppressive protein, galectin-1 emerges as a novel regulatory factor in autoimmune disorders PB - Szegedi Tudományegyetem (SZTE) PY - 2017 SP - 53 DO - 10.14232/phd.3442 UR - https://m2.mtmt.hu/api/publication/3268033 ID - 3268033 LA - English DB - MTMT ER - TY - JOUR AU - Hornung, Ákos AU - Monostori, Éva AU - Kovács, László TI - Systemic lupus erythematosus in the light of the regulatory effects of galectin-1 on T-cell function JF - LUPUS J2 - LUPUS VL - 26 PY - 2017 IS - 4 SP - 339 EP - 347 PG - 9 SN - 0961-2033 DO - 10.1177/0961203316686846 UR - https://m2.mtmt.hu/api/publication/3190069 ID - 3190069 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 18 September 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 29 November 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 2 December 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human AB - Galectin-1 is an endogenous immunoregulatory lectin-type protein. Its most important effects are the inhibition of the differentiation and cytokine production of Th1 and Th17 cells, and the induction of apoptosis of activated T-cells. Galectin-1 has been identified as a key molecule in antitumor immune surveillance, and data are accumulating about the pathogenic role of its deficiency, and the beneficial effects of its administration in various autoimmune disease models. Initial animal and human studies strongly suggest deficiencies in both galectin-1 production and responsiveness in systemic lupus erythematosus (SLE) T-cells. Since lupus features widespread abnormalities in T-cell activation, differentiation and viability, in this review the authors wished to highlight potential points in T-cell signalling processes that may be influenced by galectin-1. These points include GM-1 ganglioside-mediated lipid raft aggregation, early activation signalling steps involving p56Lck, the exchange of the CD3 zeta-ZAP-70 to the FcRgamma-Syk pathway, defective mitogen-activated protein kinase pathway activation, impaired regulatory T-cell function, the failure to suppress the activity of interleukin 17 (IL-17) producing T-cells, and decreased suppression of the PI3K-mTOR pathway by phosphatase and tensin homolog (PTEN). These findings place galectin-1 into the group of potential pathogenic molecules in SLE. LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Suhajdáné Urbán, Veronika AU - Szebeni, Gábor AU - Czibula, Ágnes AU - Blaskó, Andrea AU - Kriston-Pál, Éva AU - Makra, Ildikó AU - Hornung, Ákos AU - Szabó, Enikő AU - Uher, Ferenc AU - Than, Nándor Gábor AU - Monostori, Éva TI - Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells JF - CYTOTHERAPY J2 - CYTOTHERAPY VL - 18 PY - 2016 IS - 3 SP - 360 EP - 370 PG - 11 SN - 1465-3249 DO - 10.1016/j.jcyt.2015.12.004 UR - https://m2.mtmt.hu/api/publication/3024122 ID - 3024122 AB - BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) have powerful immunosuppressive activity. This function of MSCs is attributed to plethora of the expressed immunosuppressive factors, such as galectin-1 (Gal-1), a pleiotropic lectin with robust anti-inflammatory effect. Nevertheless, whether Gal-1 renders or contributes to the immunosuppressive effect of MSCs has not been clearly established. Therefore, this question was the focus of a complex study. METHODS: MSCs were isolated from bone marrows of wild-type and Gal-1 knockout mice and their in vitro anti-proliferative and apoptosis-inducing effects on activated T cells were examined. The in vivo immunosuppressive activity was tested in murine models of type I diabetes and delayed-type hypersensitivity. RESULTS: Both Gal-1-expressing and -deficient MSCs inhibited T-cell proliferation. Inhibition of T-cell proliferation by MSCs was mediated by nitric oxide but not PD-L1 or Gal-1. In contrast, MSC-derived Gal-1 triggered apoptosis in activated T cells that were directly coupled to MSCs, representing a low proportion of the T-cell population. Furthermore, absence of Gal-1 in MSCs did not affect their in vivo immunosuppressive effect. CONCLUSIONS: These results serve as evidence that Gal-1 does not play a role in the systemic immunosuppressive effect of MSCs. However, a local contribution of Gal-1 to modulation of T-cell response by direct cell-to-cell interaction cannot be excluded. Notably, this study serves a good model to understand how the specificity of a pleiotropic protein depends on the type and localization of the producing effector cell and its target. LA - English DB - MTMT ER - TY - JOUR AU - Hornung, Ákos AU - Deák, Magdolna AU - Novák, Julianna AU - E, Szabó AU - A, Czibula AU - R, Fajka-Boja AU - Kriston-Pál, Éva AU - Monostori, É AU - Kovács, László TI - Novel role for galectin-1 in T-cell apoptosis regulation and ist relevance to systemic lupus erythematosus JF - ANNALS OF THE RHEUMATIC DISEASES J2 - ANN RHEUM DIS VL - 74 PY - 2015 IS - Suppl. 1 SP - A19 EP - A19 SN - 0003-4967 DO - 10.1136/annrheumdis-2015-207259.44 UR - https://m2.mtmt.hu/api/publication/3040958 ID - 3040958 LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Fajka-Boja, Roberta AU - Kriston-Pál, Éva AU - Hornung, Ákos AU - Makra, Ildikó AU - Kudlik, Gyöngyi AU - Uher, Ferenc AU - Katona, Róbert László AU - Monostori, Éva AU - Czibula, Ágnes TI - Licensing by Inflammatory Cytokines Abolishes Heterogeneity of Immunosuppressive Function of Mesenchymal Stem Cell Population JF - STEM CELLS AND DEVELOPMENT J2 - STEM CELLS DEV VL - 24 PY - 2015 IS - 18 SP - 2171 EP - 2180 PG - 10 SN - 1547-3287 DO - 10.1089/scd.2014.0581 UR - https://m2.mtmt.hu/api/publication/2946565 ID - 2946565 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Szeged, 6726, Hungary Stem Cell Biology Unit, National Blood Service, Budapest, Hungary Cited By :38 Export Date: 10 February 2021 CODEN: SCDTA Correspondence Address: Czibula, A.; Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Hungary AB - When mesenchymal stem cells (MSCs) are used for therapy of immunological pathologies, they get into an inflammatory environment, altering the effectiveness of the treatment. To establish the impact of environmental inflammatory factors on MSCs' immunofunction in the mirror of intrinsic heterogeneity of mouse MSC population, individual MSC clones were generated and characterized. Adipogenic but not osteogenic differentiation and pro-angiogenic activity of five independent MSC cell lines were similar. Regarding osteogenic differentiation, clones MSC3 and MSC6 exhibited poorer capacity than MSC2, MSC4, and MSC5. To study the immunosuppressive heterogeneity, in vitro and in vivo experiments have been carried out using T-cell proliferation assay and delayed-type hypersensitivity (DTH) response, respectively. A remarkable difference was found between the clones in their ability to inhibit T-cell proliferation in the following order: MSC2MSC5>MSC4>MSC3>>MSC6. Nevertheless, the differences between the immunosuppressive activities of the individual clones disappeared on pretreatment of the cells with pro-inflammatory cytokines, a procedure called licensing. Stimulation of all clones with IFN- and TNF- resulted in elevation of their inhibitory capability to a similar level. Nitric oxide (NO) and prostaglandin E2 (PGE2) were identified as major mediators of immunofunction of the MSC clones. The earlier findings were also supported by in vivo results. Without licensing, MSC2 inhibited DTH response, while MSC6 did not affect DTH response. In contrast, prestimulation of MSC6 with inflammatory cytokines resulted in strong suppression by this clone as well. Here, we have showed that MSC population is functionally heterogeneous in terms of immunosuppressive function; however, this variability is largely reduced under pro-inflammatory conditions. LA - English DB - MTMT ER - TY - JOUR AU - Deák, Magdolna AU - Hornung, Ákos AU - Novák, Julianna AU - Demydenko, Dmytro AU - Szabó, Enikő AU - Czibula, Ágnes AU - Fajka-Boja, Roberta AU - Kriston-Pál, Éva AU - Monostori, Éva AU - Kovács, László TI - Novel role for galectin-1 in T-cells under physiological and pathological conditions JF - IMMUNOBIOLOGY J2 - IMMUNOBIOLOGY VL - 220 PY - 2015 IS - 4 SP - 483 EP - 489 PG - 7 SN - 0171-2985 DO - 10.1016/j.imbio.2014.10.023 UR - https://m2.mtmt.hu/api/publication/2807186 ID - 2807186 N1 - Cited By :19 Export Date: 13 July 2022 CODEN: ZIMMD AB - Secreted, extracellular galectin-1 (exGal-1) but not intracellular Gal-1 (inGal-1) has been described as a strong immunosuppressive protein due to its major activity of inducing apoptosis of activated T-cells. It has previously been reported that T-cells express Gal-1 upon activation, however its participation in T-cell functions has remained largely elusive. To determine function of Gal-1 expressed by activated T-cells we have carried out a series of experiments. We have shown that Gal-1, expressed in Gal-1-transgenic Jurkat cells or in activated T-cells, remained intracellularly indicating that Gal-1-induced T-cell death was not a result of an autocrine effect of the de novo expressed Gal-1. Rather, a particular consequence of the inGal-1 expression was that T-cells became more sensitive to exGal-1 added either as a soluble protein or bound to the surface of a Gal-1-secreting effector cell. This was also verified when the susceptibility of activated T-cells from wild type or Gal-1 knockout mice to Gal-1-induced apoptosis were compared. Murine T-cells expressing Gal-1 were more sensitive to the cytotoxicity of the exGal-1 than their Gal-1 knockout counterparts. We also conducted a study with activated T-cells from patients with systemic lupus erythematosus (SLE), a disease in which dysregulated T-cell apoptosis has been well described. SLE T-cells expressed lower amounts of Gal-1 than healthy T-cells and were less sensitive to exGal-1. These results suggested a novel role of inGal-1 in T-cells as a regulator of T-cell response to exGal-1, and its likely contribution to the mechanism in T-cell apoptosis deficiency in lupus. LA - English DB - MTMT ER - TY - JOUR AU - Hornung, Ákos AU - Deák, Magdolna AU - Novák, Julianna AU - Szabó, Enikő AU - Czibula, Ágnes AU - Fajka-Boja, Roberta AU - Monostori, Éva AU - Kovács, László TI - A galektin-1 T-sejtekre gyakorolt apoptotikus hatásának vizsgálata szisztémás lupus erythematosusban JF - MAGYAR REUMATOLÓGIA J2 - MAGYAR REUMATOL VL - 54 PY - 2013 IS - 3 SP - 157 EP - 157 PG - 1 SN - 0139-4495 UR - https://m2.mtmt.hu/api/publication/2703328 ID - 2703328 LA - Hungarian DB - MTMT ER -