TY - JOUR AU - Kósa, Magdolna AU - Galla, Zsolt AU - Lénárt, István AU - Baráth, Ákos AU - Grecsó, Nóra AU - Rácz, Gábor Zoltán AU - Bereczki, Csaba AU - Monostori, Péter TI - Vitamin B12 (Cobalamin): Its Fate from Ingestion to Metabolism with Particular Emphasis on Diagnostic Approaches of Acquired Neonatal/Infantile Deficiency Detected by Newborn Screening JF - METABOLITES J2 - METABOLITES VL - 12 PY - 2022 IS - 11 PG - 16 SN - 2218-1989 DO - 10.3390/metabo12111104 UR - https://m2.mtmt.hu/api/publication/33230159 ID - 33230159 AB - Acquired vitamin B12 (vB12) deficiency (vB12D) of newborns is relatively frequent as compared with the incidence of inherited diseases included in newborn screening (NBS) of different countries across the globe. Infants may present signs of vB12D before 6 months of age with anemia and/or neurologic symptoms when not diagnosed in asymptomatic state. The possibility of identifying vitamin deficient mothers after their pregnancy during the breastfeeding period could be an additional benefit of the newborn screening. Vitamin supplementation is widely available and easy to administer. However, in many laboratories, vB12D is not included in the national screening program. Optimized screening requires either second-tier testing or analysis of new urine and blood samples combined with multiple clinical and laboratory follow ups. Our scope was to review the physiologic fate of vB12 and the pathobiochemical consequences of vB12D in the human body. Particular emphasis was put on the latest approaches for diagnosis and treatment of vB12D in NBS. LA - English DB - MTMT ER - TY - CHAP AU - Monostori, Péter AU - Baráth, Ákos AU - Galla, Zsolt AU - Grecsó, Nóra AU - Bereczki, Csaba TI - Elválasztástechnika és kapcsolt módszerek a klinikai diagnosztikában T2 - METT25 a Magyar Elválasztástudományi Társaság jubileumi konferenciája PB - Magyar Elválasztástudományi Társaság CY - Pécs SN - 9786155270666 PY - 2021 SP - 36 UR - https://m2.mtmt.hu/api/publication/32923831 ID - 32923831 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Grecsó, Nóra AU - Zadori, Anita AU - Baráth, Ákos AU - Galla, Zsolt AU - Rácz, Gábor Zoltán AU - Bereczki, Csaba AU - Monostori, Péter TI - Comparison of different preparation techniques of dried blood spot quality controls in newborn screening for congenital adrenal hyperplasia JF - PLOS ONE J2 - PLOS ONE VL - 16 PY - 2021 IS - 5 PG - 11 SN - 1932-6203 DO - 10.1371/journal.pone.0252091 UR - https://m2.mtmt.hu/api/publication/32289305 ID - 32289305 N1 - Funding Agency and Grant Number: Hungarian-Romanian Cross-Border Cooperation Project (HU-RO) [0802/008 SCREENGEN]; Institutional Account Program of the University of Szeged, Szeged, Hungary [5121] Funding text: This work was supported by the Hungarian-Romanian Cross-Border Cooperation Project (HU-RO) 0802/008 SCREENGEN. Article Processing Charges were covered by the Institutional Account Program of the University of Szeged, Szeged, Hungary (Open Access Fund, Grant number: 5121). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AB - In newborn screening, samples suspected for congenital adrenal hyperplasia (CAH), a potentially lethal inborn error of steroid biosynthesis, need to be confirmed using liquid chromatography-tandem mass spectrometry. Daily quality controls (QCs) for the 2(nd)-tier CAH assay are not commercially available and are therefore generally prepared within the laboratory. For the first time, we aimed to compare five different QC preparation approaches used in routine diagnostics for CAH on the concentrations of cortisol, 21-deoxycortisol, 11-deoxycortisol, 4-androstenedione and 17-hydroxyprogesterone in dried blood spots. The techniques from Prep1 to Prep5 were tested at two analyte concentrations by spiking aliquots of a steroid-depleted blood, derived from washed erythrocyte suspension and steroid-depleted serum. The preparation processes differed in the sequence of the preparation steps and whether freeze-thaw cycles were used to facilitate blood homogeneity. The five types of dried blood spot QCs were assayed and quantitated in duplicate on five different days using a single calibration row per day. Inter-assay variations less than 15% and concentrations within +/- 15% of the nominal values were considered acceptable. Results obtained by means of the four dried blood spot QC preparation techniques (Prep1, Prep2, Prep4 and Prep5) were statistically similar and remained within the +/- 15% ranges in terms of both reproducibility and nominal values. However, concentration results for Prep3 (spiking prior to three freeze-thaw cycles) were significantly lower than the nominal values in this setting, with differences exceeding the +/- 15% range in many cases despite acceptable inter-assay variations. These findings have implications for the in-house preparation of QC samples in laboratory developed tests for CAH, including 2(nd)-tier assays in newborn screening. LA - English DB - MTMT ER - TY - JOUR AU - Galla, Zsolt AU - Rácz, Gábor Zoltán AU - Grecsó, Nóra AU - Baráth, Ákos AU - Kósa, Magdolna AU - Bereczki, Csaba AU - Monostori, Péter TI - Improved LC-MS/MS method for the determination of 42 neurologically and metabolically important molecules in urine JF - JOURNAL OF CHROMATOGRAPHY B J2 - J CHROMATOGR B VL - 1179 PY - 2021 PG - 8 SN - 1570-0232 DO - 10.1016/j.jchromb.2021.122846 UR - https://m2.mtmt.hu/api/publication/32092570 ID - 32092570 N1 - Export Date: 23 February 2022 CODEN: JCBAA Correspondence Address: Galla, Z.; Metabolic and Newborn Screening Laboratory, Korányi fasor 14-15, H-6720, Hungary; email: galla.zsolt@med.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Galla, Zsolt AU - Rajda, Cecília AU - Rácz, Gábor Zoltán AU - Grecsó, Nóra AU - Baráth, Ákos AU - Vécsei, László AU - Bereczki, Csaba AU - Monostori, Péter TI - Simultaneous determination of 30 neurologically and metabolically important molecules: A sensitive and selective way to measure tyrosine and tryptophan pathway metabolites and other biomarkers in human serum and cerebrospinal fluid JF - JOURNAL OF CHROMATOGRAPHY A J2 - J CHROMATOGR A VL - 1635 PY - 2021 PG - 13 SN - 0021-9673 DO - 10.1016/j.chroma.2020.461775 UR - https://m2.mtmt.hu/api/publication/31749099 ID - 31749099 N1 - Metabolic and Newborn Screening Laboratory, Department of Paediatrics, University of Szeged Department of Neurology, University of Szeged, Hungary Department of Neurology, MTA-SZTE Neuroscience Research Group, Interdisciplinary Excellence Centre, Faculty of Medicine, Universtiy of Szeged, Hungary Cited By :2 Export Date: 6 September 2021 CODEN: JCRAE Correspondence Address: Galla, Z.; Metabolic and Newborn Screening Laboratory, email: galla.zsolt@med.u-szeged.hu Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Biomarkers; Tryptophan; Tyrosine Funding details: 2.3.2-15-2016-00034, TUDFO/47138-1/2019-ITM Funding text 1: Anita Zádori and Dalma Szabó for the technical assistance in sample preparations. Gabriella Vörösné Keszég and Csilla Szabó for their technical assistance in biobanking. László Vécsei was supported by GINOP 2.3.2-15-2016-00034 and TUDFO/47138-1/2019-ITM. Metabolic and Newborn Screening Laboratory, Department of Paediatrics, University of Szeged Department of Neurology, University of Szeged, Hungary Department of Neurology, MTA-SZTE Neuroscience Research Group, Interdisciplinary Excellence Centre, Faculty of Medicine, Universtiy of Szeged, Hungary Cited By :2 Export Date: 7 September 2021 CODEN: JCRAE Correspondence Address: Galla, Z.; Metabolic and Newborn Screening Laboratory, email: galla.zsolt@med.u-szeged.hu Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Biomarkers; Tryptophan; Tyrosine Funding details: 2.3.2-15-2016-00034, TUDFO/47138-1/2019-ITM Funding text 1: Anita Zádori and Dalma Szabó for the technical assistance in sample preparations. Gabriella Vörösné Keszég and Csilla Szabó for their technical assistance in biobanking. László Vécsei was supported by GINOP 2.3.2-15-2016-00034 and TUDFO/47138-1/2019-ITM. LA - English DB - MTMT ER - TY - JOUR AU - Grecsó, Nóra AU - Zádori, Anita AU - Szécsi, Ilona AU - Baráth, Ákos AU - Galla, Zsolt AU - Bereczki, Csaba AU - Monostori, Péter TI - Storage stability of five steroids and in dried blood spots for newborn screening and retrospective diagnosis of congenital adrenal hyperplasia JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 5 PG - 10 SN - 1932-6203 DO - 10.1371/journal.pone.0233724 UR - https://m2.mtmt.hu/api/publication/31332527 ID - 31332527 LA - English DB - MTMT ER - TY - THES AU - Grecsó, Nóra TI - Investigation of Novel Cinchona Alkaloid-based Zwitterionic Chiral Stationary Phases in Cation- and Zwitterion-Exchange Modes PB - Szegedi Tudományegyetem (SZTE) PY - 2017 SP - 52 DO - 10.14232/phd.3973 UR - https://m2.mtmt.hu/api/publication/3266894 ID - 3266894 LA - English DB - MTMT ER - TY - JOUR AU - Orosz, Tímea AU - Grecsó, Nóra AU - Lajkó, Gyula AU - Szakonyi, Zsolt AU - Fülöp, Ferenc AU - Armstrong, DW AU - Ilisz, István AU - Péter, Antal TI - Liquid chromatographic enantioseparation of carbocyclic β-amino acids possessing limonene skeleton on macrocyclic glycopeptide-based chiral stationary phases JF - JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS J2 - J PHARMACEUT BIOMED ANAL VL - 145 PY - 2017 SP - 119 EP - 126 PG - 8 SN - 0731-7085 DO - 10.1016/j.jpba.2017.06.010 UR - https://m2.mtmt.hu/api/publication/3256483 ID - 3256483 N1 - ISSN:0731-7085 AB - Polar-ionic and reversed-phase high-performance liquid chromatographic separations of limonene-based cyclic β-amino acid enantiomers were carried out by using macrocyclic glycopeptide-based chiral selectors applying Chirobiotic T, TAG and R columns. The effects of additives, concentration of the co- and counter-ions and the temperature in polar-ionic mobile phase systems were studied. The influence of pH, MeOH content and alcohol additives were investigated in the reversed-phase mode. The difference in the change in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) was calculated from the linear van't Hoff plots derived from the ln α vs 1/T curves in the temperature range 5–40 °C. Unusual temperature behavior was observed on Chirobiotic TAG for most of the analytes: decreased retention times were accompanied with increased separation factors with increasing temperature, and separation was entropically-driven. For two of the studied analytes enthalpically-driven enantioseparations were observed. The elution sequence was determined in all cases, but no general rule could be established. © 2017 Elsevier B.V. LA - English DB - MTMT ER - TY - JOUR AU - Lajkó, Gyula AU - Grecsó, Nóra AU - Tóth, Gábor AU - Fülöp, Ferenc AU - Lindner, Wolfgang AU - Ilisz, István AU - Péter, Antal TI - Liquid and subcritical fluid chromatographic enantioseparation of Nα-Fmoc proteinogenic amino acids on Quinidine-based zwitterionic and anion-exchanger type chiral stationary phases. A comparative study. JF - CHIRALITY: THE PHARMACOLOGICAL BIOLOGICAL AND CHEMICAL CONSEQUENCES OF MOLECULAR ASYMMETRY J2 - CHIRALITY VL - 29 PY - 2017 IS - 6 SP - 225 EP - 238 PG - 14 SN - 0899-0042 DO - 10.1002/chir.22700 UR - https://m2.mtmt.hu/api/publication/3244835 ID - 3244835 N1 - N1 CAPLUS AN 2017:623398(Journal; Online Computer File) Funding Agency and Grant Number: Hungarian National Science Foundation [OTKA K 108847] Funding text: Hungarian National Science Foundation, Grant/Award Number: OTKA K 108847 AB - Stereoselective high-performance liq. chromatog. and subcrit. fluid chromatog. sepns. of 19 Nα-Fmoc proteinogenic amino acid enantiomers were carried out by using Quinidine-based zwitterionic and anion-exchanger-type chiral stationary phases Chiralpak ZWIX(-) and QD-AX. For optimization of retention and enantioselectivity, the ratio of bulk solvent components (MeOH/MeCN, H2O/MeOH, or CO2/MeOH) and the nature and concn. of the acid and base additives (counter- and co-ions) were systematically varied. The effect of column temp. on the enantiosepn. was investigated and thermodn. parameters were calcd. from the van't Hoff plots ln α vs. 1/T. The thermodn. parameters revealed that the enantiosepns. were enthalpy-driven. The elution sequence was detd. in all cases and with the exception of Fmoc-Cys(Trt)-OH, it was identical on both chiral stationary phases whereby the L-enantiomers eluted before the D-enantiomers. [on SciFinder(R)] LA - English DB - MTMT ER - TY - JOUR AU - Lajkó, Gyula AU - Grecsó, Nóra AU - Tóth, Gábor AU - Fülöp, Ferenc AU - Lindner, W AU - Péter, Antal AU - Ilisz, István TI - A comparative study of enantioseparations of Nα-Fmoc proteinogenic amino acids on Quinine-based zwitterionic and anion exchanger-type chiral stationary phases under hydro-organic liquid and subcritical fluid chromatographic conditions JF - MOLECULES J2 - MOLECULES VL - 21 PY - 2016 IS - 11 PG - 19 SN - 1420-3049 DO - 10.3390/molecules21111579 UR - https://m2.mtmt.hu/api/publication/3154880 ID - 3154880 N1 - Funding Agency and Grant Number: Hungarian National Science Foundation [OTKA K 108847] Funding text: This work was supported by Hungarian National Science Foundation grant OTKA K 108847. A.P. gratefully thanks Pilar Franco (Chiral Technologies Europe) for providing the Chiralpak columns. The authors are grateful to Waters Kft (Budapest, Hungary) for the loan of the Acquity UPC2 system. AB - The focus of this contribution is a comparative investigation of enantioseparations of 19 Nα-Fmoc proteinogenic amino acids on Quinine-based zwitterionic and anion-exchanger type chiral stationary phases employing hydro-organic and polar-ionic liquid and subcritical fluid chromatographic conditions. Effects of mobile phase composition (including additives, e.g., water, basis and acids) and nature of chiral selectors on the chromatographic performances were studied at different chromatographic modes. Thermodynamic parameters of the temperature dependent enantioseparation results were calculated in the temperature range 5-50°C applying plots of lnα versus 1/T. The differences in standard enthalpy and standard entropy for a given pair of enantiomers were calculated and served as a basis for comparisons. Elution sequence in all cases was determined, where a general rule could be observed, both in liquid and subcritical fluid chromatographic mode the D-enantiomers eluted before the L ones. In both modes, the principles of ion exchange chromatography apply. © 2016 by the authors; licensee MDPI. LA - English DB - MTMT ER -