TY - JOUR AU - Becze, Annamária AU - Vincze, Éva-Boglárka AU - Lányi, Szabolcs AU - Mara, G. TI - EVALUATION OF STRUCTURAL CARBOHYDRATE DEGRADING CAPACITY OF PGP BACTERIAL STRAINS USING DIFFERENT METHODS JF - UPB SCIENTIFIC BULLETIN, SERIES B: CHEMISTRY AND MATERIALS SCIENCE J2 - UPB SCI BULL SER B VL - 84 PY - 2022 IS - 1 SP - 3 EP - 18 PG - 16 SN - 1454-2331 UR - https://m2.mtmt.hu/api/publication/32772087 ID - 32772087 LA - English DB - MTMT ER - TY - JOUR AU - Bartos, Hunor AU - Balázs, Márta AU - Kuzman, Ildikó Hajnalka AU - Lányi, Szabolcs AU - Miklóssy, Ildikó TI - Production of High Added-Value Chemicals in Basfia succiniciproducens: Role of Medium Composition JF - SUSTAINABILITY J2 - SUSTAINABILITY-BASEL VL - 13 PY - 2021 IS - 6 PG - 12 SN - 2071-1050 DO - 10.3390/su13063513 UR - https://m2.mtmt.hu/api/publication/31936624 ID - 31936624 LA - English DB - MTMT ER - TY - PAT AU - Albert, Beáta AU - Lányi, Szabolcs AU - Máthé, István AU - Laslo, Éva AU - Orbán, Kálmán Csongor AU - Bodor, Zsolt AU - Tánczos, Szidónia AU - Fejér, Király Gergely AU - Koncz, Mihály AU - Tóro, Szabolcs AU - Funkenhauser, Bernadett AU - Dobri, Emőke TI - Lactobacillus plantarum subsp. plantarum használata haszonnövények silózási folyamataiban PY - 2019 UR - https://m2.mtmt.hu/api/publication/31913311 ID - 31913311 LA - Romanian DB - MTMT ER - TY - PAT AU - Albert, Beáta AU - Lányi, Szabolcs AU - Mara, Gyöngyvér AU - Kovács, Erika AU - Orbán, Kálmán Csongor AU - Laslo, Éva AU - Bálint, Emese AU - Bodor, Zsolt AU - Mészáros, Alexandru AU - Kádár, Attila AU - Sinkler, Réka AU - Toro, Szabolcs AU - Funkenhauser, Bernadett AU - Becze, Annamária TI - Az sp. SZX 102 Bacillus törzs használata a haszonnövények silózási folyamataiban PY - 2019 UR - https://m2.mtmt.hu/api/publication/31913310 ID - 31913310 LA - Romanian DB - MTMT ER - TY - PAT AU - Albert, Beáta AU - Bálint, Emese AU - Becze, Annamária AU - Bodor, Zsolt AU - Dobri, Emőke AU - Fejér, Király Gergely AU - Koncz, Mihály AU - Kovács, Erika AU - Lányi, Szabolcs AU - Laslo, Éva AU - Mara, Gyöngyvér AU - Máthé, István AU - Máthé, Loránd AU - Mészáros, Alexandru AU - Orbán, Kálmán Csongor AU - Tánczos, Szidónia TI - Az sp. SZE 102A Bacillus törzs használata a haszonnövények silózási folyamataiban PY - 2019 UR - https://m2.mtmt.hu/api/publication/31913285 ID - 31913285 LA - Romanian DB - MTMT ER - TY - CONF AU - Salamon, Pál Attila AU - Miklóssy, Ildikó AU - Lányi, Szabolcs AU - Orbán, Kálmán Csongor AU - Albert, Beáta ED - Majdik, Cornelia TI - Fehérje-fehérje interakció vizsgálata a sejthalál szabályozásában T2 - 25th International Conference on Chemistry = XXV. Nemzetközi Vegyészkonferencia PB - Erdélyi Magyar Műszaki Tudományos Társaság (EMT) C1 - Kolozsvár T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 T3 - Nemzetközi Vegyészkonferencia, ISSN 1843-6293 PY - 2019 SP - 105 UR - https://m2.mtmt.hu/api/publication/31356193 ID - 31356193 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Nagy, Katalin AU - Orbán, Kálmán Csongor AU - Albert, Beáta AU - Lányi, Szabolcs TI - Biosynthesis BIR3 domain of inhibitor of apoptosis proteins JF - UPB Sci. Bull., series B VL - 81 PY - 2019 IS - 4 SP - 1454 EP - 2331 PG - 878 UR - https://m2.mtmt.hu/api/publication/31239040 ID - 31239040 LA - English DB - MTMT ER - TY - JOUR AU - Bodor, Zsolt AU - Lányi, Szabolcs AU - Albert, Beáta AU - Bodor, Katalin AU - Aurelia, Cristina Nechifor AU - Miklóssy, Ildikó TI - Model driven analysis of the biosynthesis of 1,4-butanediol from renewable feedstocks in Escherichia coli JF - REVISTA DE CHIMIE J2 - REV CHIM-BUCHAREST VL - 70 PY - 2019 IS - 11 SP - 3808 EP - 3817 PG - 10 SN - 0034-7752 DO - 10.37358/rc.70.19.11.7651 UR - https://m2.mtmt.hu/api/publication/30767357 ID - 30767357 N1 - Sapientia Hungarian University of Transylvania, Faculty of Economics,, Socio-Human Sciences and Engineering, Department of Bioengineering, 1 Libertatii Sq., Miercurea Ciuc, 530104, Romania University of Pecs, Faculty of Natural Sciences, Doctoral School of Chemistry, Ifjusag 6, 7624, Pecs, Hungary Heineken Romania, 100 Harghita Str., Miercurea Ciuc, 530154, Romania Politehnica University of Bucharest, Faculty of Applied Chemistry and Material Science, Analytical Chemistry and Environmental Engineering Department, 1-7 Gheorghe Polizu Str., Bucharest, 011061, Romania Export Date: 14 February 2020 CODEN: RCBUA Correspondence Address: Bodor, Z.; Sapientia Hungarian University of Transylvania, Faculty of Economics,, Socio-Human Sciences and Engineering, Department of Bioengineering, 1 Libertatii Sq., Romania; email: bodorzsolt@uni.sapientia.ro LA - English DB - MTMT ER - TY - JOUR AU - Sinkler, Réka AU - Balázs, Márta AU - Antal, Emőke AU - Bartos, Hunor AU - Lányi, Szabolcs AU - Miklóssy, Ildikó TI - METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS JF - STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA J2 - STUD UNIV BABES-BOLYAI CHEM VL - 64 PY - 2019 IS - 2 SP - 159 EP - 174 PG - 16 SN - 1224-7154 DO - 10.24193/subbchem.2019.2.13 UR - https://m2.mtmt.hu/api/publication/30765679 ID - 30765679 N1 - Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Gheorghe Polizu str, Bucharest, RO-011061, Romania University of Pécs, Faculty of Natural Sciences, Doctoral School of Chemistry, 6 Ifjúság str, Pécs, Hu-7624, Hungary Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Miercurea Ciuc, RO-530104, Romania Export Date: 4 November 2019 Correspondence Address: Miklóssy, I.; Sapientia Hungarian University of Transylvania, Faculty of Economics, Socio-Human Science and Engineering, Department of Bioengineering, 1 Libertatii sqr, Romania; email: miklossyildiko@uni.sapientia.ro AB - 1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enzymes in a heterologous pathway leading to BDO production, which were introduced into a genome-engineered E. coli MG1655(DE3) Delta ldhA, Delta pflB strain. Knowing that the expression of recombinant proteins and gene deletions can significantly influence cellular viability, the present study was carried out to investigate the impact of the two key enzyme expression on deletion strains, helping us to analyze the physiological changes of E. coli strains and providing directions for further optimizations in order to achieve satisfying target product yields. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Katalin AU - Kovács, Zita AU - Salamon, Pál Attila AU - Orbán, Kálmán Csongor AU - Lányi, Szabolcs AU - Albert, Beáta TI - ENHANCED HETEROLOGOUS EXPRESSION IN E. COLI JF - STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA J2 - STUD UNIV BABES-BOLYAI CHEM VL - 64 PY - 2019 IS - 2 SP - 101 EP - 110 PG - 10 SN - 1224-7154 DO - 10.24193/subbchem.2019.2.09 UR - https://m2.mtmt.hu/api/publication/30765654 ID - 30765654 N1 - University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science, Str. Gh. Polizu, Nr. 1-7, Sector 1, Bucuresti, 011061, Romania University of Pécs, Faculty of Sciences, Ifjúság útja 6, Pécs, 7624, Hungary SAPIENTIA Hungarian University of Transylvania, Faculty of Economics, Socio-Human Sciences and Engineering, Department of Bioengineering, 1 Libertatii Square, Miercurea Ciuc, RO-530104, Romania Export Date: 4 November 2019 Correspondence Address: Orbán, C.-K.; SAPIENTIA Hungarian University of Transylvania, Faculty of Economics, Socio-Human Sciences and Engineering, Department of Bioengineering, 1 Libertatii Square, Romania; email: orbancsongor@uni.sapientia.ro AB - Apoptotic regulation has been implicated in many human diseases, including cancer, autoimmune disease, inflammation and neuro degradation. Mapping up critical apoptosis regulators is a strategy for the development of new therapies [1, 2].Present work highlights optimization of heterologous expression conditions for the X-linked inhibitor of apoptosis protein (XIAP). Genes of target protein containing pGEX-4T vector was transformed in chemically competent E. coli Rosetta (TM)(DE3)pLysS cells. The recombinant construct contained a glutathione S-transferase (GST) fusion partner, which assured the purification of the protein by affinity chromatography. In the next step we examined the growth dynamics of the expression culture in M9 minimal medium, meanwhile we also determined the appropriate time of induction. Following this we carried out the optimization of expression, examining the expression's effectiveness under different conditions. On the basis of these fermentation experiments the target protein expression was the most prominent at 18 degrees C with 0.2 mM IPTG induction for 12 hours. During large scale fermentation experiments, we followed the optical density (OD), dry cell weight and substrate utilization. Finally, recombinant protein expression inhancement in the presence of 3% ethanol was successfully achieved in bioreactor. In this case the target protein was expressed in inclusion bodies, therefore solubilisation and refolding is necessary. LA - English DB - MTMT ER -